Scanning Electron Microscopy Techniques in the Analysis of Gunshot Residues: A Literature Review
Source abstract
Abstract
The analysis of gunshot residues (GSRs) is a critical component of criminal investigations, linking suspects to firearms or shooting incidents. Among the various analytical techniques employed, scanning electron microscopy (SEM) has emerged as a valuable tool due to its ability to provide high-resolution imaging and detailed elemental composition analysis of GSR particles. Recent technological advancements have significantly enhanced the effectiveness of SEM in GSR analysis, incorporating improved detectors and software that facilitate the more accurate detection and characterization of GSR particles. To ensure the reliability of SEM-based GSR analysis, it is essential to adhere to established methodologies for sample collection and preparation, as well as to implement best practices in data interpretation within the forensic context. Through a narrative review, this paper aims to explore the application of SEM techniques for GSR analysis, elucidate the methodological approaches that underpin effective forensic investigations, and highlight the advantages and limitations of SEM, thereby addressing the ongoing challenges and opportunities in the field.
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Research context
OpenAlex topics: Integrated Circuits and Semiconductor Failure Analysis · Forensic Fingerprint Detection Methods · Ion-surface interactions and analysis
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Affiliations
Institute of Legal Medicine, Department of Medical and Surgical Sciences, University “Magna Graecia” of Catanzaro, Viale Europa, Loc. Germaneto, 88100 Catanzaro, Italy
Instituto Nacional de Medicina Legal e Ciencias Forenses, Jardim Carrilho Videira, 4050-167 Porto, Portugal
School of Medicine and Biomedical Sciences, University of Porto, 4169-007 Porto, Portugal
References (63)
Show source-provided references
1. Goldstein, J., Newbury, D.E., Joy, D.C., Lyman, C.E., Echlin, P., Lifshin, E., Sawyer, L., and Michael, J.R. (2017). Scanning Electron Microscopy and X-Ray Microanalysis, Springer.
2. Kröner, A., and Hirsch, T. (2020). Current Trends in the Optical Characterization of Two-Dimensional Carbon Nanomaterials. Front. Chem., 7.10.3389/fchem.2019.00927 ↗
3. Reimer, L., and Kohl, H. (2013). Transmission Electron Microscopy: Physics of Image Formation, Springer.
4. Williams, D.B., and Carter, C.B. (2009). Transmission Electron Microscopy: A Textbook for Materials Science, Springer.10.1007/978-0-387-76501-3 ↗
5. Brinza, C.A., Salceanu, M., Melian, A., Taraboanta, I., Cimpoesu, R., Giuroiu, C.L., Sandu, I., and Andrian, S. (2025). Evaluation by Scanning Electron Microscopy and Energy Dispersive X-Ray Spectroscopy of the Effects of Root Canal Instrumentation on the Radicular Dentine. Microsc. Res. Tech.10.1002/jemt.24763 ↗
6. Scanning Electron Microscopy Coupled with Energy Dispersive Spectroscopy Applied to the Analysis of Fibers and Particles in Tissues from Colon Adenocarcinomas
7. Din, M.S.U., Naveed, M., Aziz, T., Makhdoom, S.I., Waseem, M., Khan, A.A., Al-Harbi, M., and Alasmari, A.F. (2025). Comprehensive Characterization and Bioactivity Assessment of Titanium Dioxide Nanoparticles Synthesized Using Chrysanthemum indicum Leaf Extract. Chem. Biodivers.10.1002/cbdv.202500244 ↗
8. Zhao, J., Yang, H., and Sui, Y. (2025). Computer Vision-Based Automatic Evaluation Method of Y2O3 Steel Coating Performance with SEM Image. Sci. Rep., 15.10.1038/s41598-024-85061-0 ↗
9. Combined Use of Scanning Electron Microscopy-Energy-Dispersive X-ray Spectroscopy and Fourier Transform Infrared Imaging Coupled with Principal Component Analysis in the Study of Ancient Egyptian Papyri10.1021/acsomega.9b03145 ↗
10. Kang, J.K., Yoon, S.J., Park, H., Lee, S.J., Baek, J., Jeon, I.Y., and Gwak, S.J. (2024). Characterization of Antimicrobial Properties of Copper-Doped Graphitic Nanoplatelets. Int. J. Mol. Sci., 25.10.3390/ijms252212414 ↗
11. The Role of High-Resolution Transmission Electron Microscopy and Aberration-Corrected Scanning Transmission Electron Microscopy in Unraveling the Structure-Property Relationships of Pt-Based Fuel Cells Electrocatalysts
12. Winterroth, F., Wang, J., Wink, O., Carelsen, B., Dahl, J., and Thakor, A.S. (2025). A Theoretical Approach in Applying High-Frequency Acoustic and Elasticity Microscopy to Assess Cells and Tissues. Annu. Rev. Biomed., 27.10.1146/annurev-bioeng-112823-103134 ↗
13. Unveiling Lodderomyces elongisporus as an Emerging Yeast Pathogen: A Holistic Approach to Microbiological Diagnostic Strategies10.1007/s11046-024-00901-x ↗
14. Four-Dimensional Printing Hierarchy Scaffolds with Highly Biocompatible Smart Polymers for Tissue Engineering Applications10.1089/ten.tec.2015.0542 ↗
15. Quantitative Analysis of Keratin Filament Networks in Scanning Electron Microscopy Images of Cancer Cells10.1111/j.1365-2818.2005.01505.x ↗
16. Pilot Application of SEM/EDX Analysis on Suspected Cigarette Burns in a Forensic Autopsy Case of Child Abuse10.1097/PAF.0000000000000922 ↗
17. Police Vehicle Contamination by Inorganic Gunshot Residue (iGSR) in Zagreb County Police Administration (Croatia): Analysis of Characteristic and Indicative Particles Across Different Vehicle Parts and Contributory Risk Factors10.1111/1556-4029.15628 ↗
18. Microscopical Characterization of Known Postmortem Root Bands Using Light and Scanning Electron Microscopy10.1016/j.forsciint.2016.07.009 ↗
19. Colorimetric Barium Detection of Gunshot Residues on Cadaveric Human Skin: A Pilot Application for Forensic Purposes10.1177/19253621241240162 ↗
20. Use of Luminescent Gunshot Residues Markers in Forensic Context-Part II10.1016/j.forsciint.2017.09.022 ↗
21. Identification of Gunshot Residue: A Critical Review10.1016/S0379-0738(00)00428-X ↗
22. Particle Analysis for the Detection of Gunshot Residue. I: Scanning Electron Microscopy/Energy Dispersive X-Ray Characterization of Hand Deposits from Firing10.1520/JFS10848J ↗
23. ENFSI Proficiency Test Program on Identification of GSR by SEM/EDX10.1520/JFS2002396 ↗
24. Salucci, S., Traversari, M., Valentini, L., Versari, I., Ventura, L., Giampalma, E., Righi, E., Petrella, E., Gobbi, P., and Pasquinelli, G. (2025). The Role of 3D Virtual Anatomy and Scanning Environmental Electron Microscopy in Understanding Morphology and Pathology of Ancient Bodies. Tomography, 11.10.3390/tomography11010005 ↗
25. Identifying Sample Provenance from SEM/EDS Automated Particle Analysis via Few-Shot Learning Coupled with Similarity Graph Clustering
26. A Study on the Correlation of the Physico-Chemical Properties of Gunshot Residue (GSR) Particles with Distance Using a 9×19 mm Indian Ammunition10.1007/s12024-024-00826-3 ↗
27. Serol, M., Ahmad, S.M., Quintas, A., and Família, C. (2023). Chemical Analysis of Gunpowder and Gunshot Residues. Molecules, 28.10.3390/molecules28145550 ↗
28. Automated Segmentation of Cell Organelles in Volume Electron Microscopy Using Deep Learning10.1002/jemt.24548 ↗
29. Automated and Autonomous Experiments in Electron and Scanning Probe Microscopy10.1021/acsnano.1c02104 ↗
30. Kapun, G., Majorovits, E., Šturm, S., Marinšek, M., and Skalar, T. (2024). The Microstructural Reconstruction of Variously Sintered Ni-SDC Cermets Using Focused Ion Beam Scanning Electron Microscopy Nanotomography. Materials, 17.10.2139/ssrn.4788946 ↗
31. Automated Large Volume Sample Preparation for vEM10.1016/bs.mcb.2023.01.009 ↗
32. From Nano to Micrometer Size Particles—A Characterization of Airborne Cement Particles During Construction Activities10.1016/j.jhazmat.2020.122838 ↗
33. Zappa, D. (2019). The Influence of Nb on the Synthesis of WO3 Nanowires and the Effects on Hydrogen Sensing Performance. Sensors, 19.10.3390/s19102332 ↗
34. Compact Vacuum Transfer Devices for Highly Air-Sensitive Materials in Scanning Electron Microscopy10.1016/j.micron.2024.103720 ↗
35. A New Compact Potentiometric Electrode for pH Monitoring Built Upon a Glass Substrate with a Ce-Doped SnO2 Layer10.1039/D4AY01161A ↗
36. Beyond the shot: Exploring secondary transfer of gunshot residue on common surfaces and the impact of hand cleaning methods10.1016/j.forsciint.2024.112135 ↗
37. Organic and inorganic gunshot residues on the hands, forearms, face, and nostrils of shooters 30 min after a discharge10.1016/j.scijus.2024.08.002 ↗
38. Ear as an alternative sampling site for GSR analysis following shotgun discharge
39. Real-time detection of GSR particles from crime scene: A comparative study of SEM/EDX and portable LIBS system10.1016/j.forsciint.2018.09.021 ↗
40. Combined collection and analysis of inorganic and organic gunshot residues10.1111/1556-4029.14314 ↗
41. Classification Improvements in Automated Gunshot Residue (GSR) Scans10.1111/1556-4029.13711 ↗
42. Conduction of a round-robin test on a real sample for the identification of gunshot residues by SEM/EDX10.1016/j.forsciint.2020.110183 ↗
43. The Application and Development of Electron Microscopy for Three-Dimensional Reconstruction in Life Science: A Review10.1007/s00441-024-03878-7 ↗
44. Observation of Biological and Emulsion Samples by Newly Developed Three-Dimensional Impedance Scanning Electron Microscopy10.1016/j.csbj.2024.11.023 ↗
45. Realizing Smart Scanning Transmission Electron Microscopy Using High Performance Computing10.1063/5.0225401 ↗
46. Recent Advances in the Characterization of Hair of Mummies from the Chilean Andean Coast10.1016/j.forsciint.2015.01.005 ↗
47. Brożek-Mucha, Z. (2014). Scanning electron microscopy and X-ray microanalysis for chemical and morphological characterisation of the inorganic component of gunshot residue: Selected problems. Biomed. Res. Int., 2014.10.1155/2014/428038 ↗
48. Interpol review of gunshot residue 2016–201910.1016/j.fsisyn.2020.01.011 ↗
49. Investigating gunshot wounds in charred bone with XRF spectroscopy: A technical note10.1007/s00414-024-03274-4 ↗
50. False-negative probability in the SEM/EDS automated discovery of iGSR particles: A Bayesian approach10.1111/1556-4029.15323 ↗
51. A study of dispersion of gunshot residue from a frequently used Serbian ammunition cal. 7.65 mm to support selected aspects of casework in North Macedonia10.1016/j.scijus.2023.04.004 ↗
52. Ansari, N., Dasgupta, J., Umre, S., and Rajput, P. (2024). Harnessing Electron Microscope for Trace Evidence Analysis, IntechOpen.10.5772/intechopen.1004112 ↗
53. Should inorganic or organic gunshot residues be analysed first?10.1016/j.forsciint.2023.111600 ↗
54. A likelihood-ratio framework for evaluating results of forensic gunshot-residue analysis10.1016/j.forsciint.2022.111339 ↗
55. An application example of the likelihood ratio approach to the evaluation of organic gunshot residues using a fictional scenario and recently published data10.1016/j.forsciint.2022.111267 ↗
56. Proposed practices for validating the performance of instruments used for automated inorganic gunshot residue analysis10.1016/j.forc.2020.100252 ↗
57. A study of the effect of working parameters and validation of SEM/EDS method for determination of elemental composition of commonly encountered GSR samples in shooting events in Kosovo10.1111/1556-4029.14803 ↗
58. Estimation of the firing distance through micro-CT analysis of gunshot wounds10.1007/s00414-010-0533-6 ↗
59. Gunshot residue testing in suicides: Part I: Analysis by scanning electron microscopy with energy-dispersive X-ray10.1097/PAF.0b013e31806195e1 ↗
60. Pagano, F., Vincenti, F., Montesano, C., Fanti, F., Gregori, A., Curini, R., and Sergi, M. (2022). Oral fluid as a new investigative matrix for the determination of organic gunshot residue exposure. J. Chromatogr. B Anal. Technol. Biomed. Life Sci., 1210.10.1016/j.jchromb.2022.123477 ↗
61. Analysis of interactions between proteins and small-molecule drugs by a biosensor based on a graphene field-effect transistor
62. Liu, G., Wei, J., Li, X., Tian, M., Wang, Z., Shen, C., Sun, W., Li, C., Li, X., and Lv, E. (2022). Near-Infrared-Responded High Sensitivity Nanoprobe for Steady and Visualized Detection of Albumin in Hepatic Organoids and Mouse Liver. Adv. Sci., 9.10.1002/advs.202202505 ↗
63. Drug evaluation platform based on non-destructive and real-time in situ organoid fate state monitoring by graphene field-effect transistor10.1016/j.cej.2024.155355 ↗