Evaluation of the Effectiveness of Bioaugmentation-Assisted Phytoremediation of Soils Contaminated with Petroleum Hydrocarbons Using Echinacea purpurea
Source abstract
Abstract
Phytoremediation supported by bioaugmentation is a promising method considered for cleaning up areas polluted with petroleum hydrocarbons. In this study, phytoremediation was carried out using Echinacea purpurea as a phytoremediant on two types of soil: Soil DW—aged soil taken from an excavation pit, Soil OS—soil taken from an oil spill area. The tests for each soil were carried out in six test systems (non-inoculation, inoculation with the B1 microbial consortium, inoculation with the B2 microbial consortium, inoculation with the B1 microbial consortium with the addition of γ-PGA (γ-poly glutamic acid), inoculation with the B2 microbial consortium with the addition of γ-PGA and inoculation with the γ-PGA solution) for 6 months. The effectiveness of the remediation treatments used was assessed based on chromatographic analyses of soil and plant material (roots, shoots) and toxicological analyses using four types of toxicological tests (PhytotoxkitTM (MicroBioTests Inc., Gent, Belgium), OstracodtoxkitTM (MicroBioTests Inc., Gent, Belgium), Microtox® Solid Phase Test (Modern Water Inc., New Castle, DE, USA), MARA (NCIMB Ltd., Aberdeen, UK)). The research conducted showed that the most effective method of bioremediation of soils contaminated with petroleum hydrocarbons was phytoremediation supported by bioaugmentation with the microbial consortium B2 with γ-PGA, which allowed for reducing the concentration of total petroleum hydrocarbons (TPHs) and polycyclic aromatic hydrocarbons (PAHs) in the tested soils by 53.98% and 49.54% (Soil DW-5) and 60.47% and 37.55% (Soil OS-5), respectively. In turn, the lowest bioremediation efficiency was recorded in non-inoculated systems, for which the concentration of TPHs and PAHs at the end of the study decreased by 18.40% and 16.14% (Soil DW-1) and 21.87% and 18.20% (Soil OS-1), respectively. The results of toxicological analyses confirmed the relationship between the concentration of TPHs and PAHs in the soil and its toxicity level.
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Research context
OpenAlex topics: Microbial bioremediation and biosurfactants · Chemical Reactions and Isotopes · Carcinogens and Genotoxicity Assessment
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Oil and Gas Institute—National Research Institute, ul. Lubicz 25 A, 31-503 Krakow, Poland
Funding
Polish Ministry of Education and Science
References (91)
Show source-provided references
1. Steliga, T. (2009). Bioremediacja Odpadów Wiertniczych Zanieczyszczonych Substancjami Ropopochodnymi ze Starych Dołów Urobkowych, Instytut Nafty i Gazu.
2. Efektywna Metoda Identyfikacji Zanieczyszczeń Ropopochodnych (TPH) i Wielopierścieniowych Węglowodorów Aromatycznych (WWA) w Glebach10.18668/NG.2017.07.06 ↗
3. Badania Laboratoryjne Wpływu Dodatku γ-PGA Na Efektywność Biodegradacji Węglowodorów Ropopochodnych10.18668/NG.2022.09.04 ↗
4. Tang, K.H.D. (2023). Phytoremediation: Where Do We Go from Here?. Biocatal. Agric. Biotechnol., 50.10.1016/j.bcab.2023.102721 ↗
5. Molecular Mechanisms in Phytoremediation of Environmental Contaminants and Prospects of Engineered Transgenic Plants/Microbes10.1016/j.scitotenv.2019.135858 ↗
6. Phytoremediation of Contaminated Soils Using Ornamental Plants10.1139/er-2017-0022 ↗
7. Toksyczność gleby zanieczyszczonej olejem silnikowym poddanej samooczyszczaniu oraz fitoremediacji
8. Plant–Bacteria Partnerships for the Remediation of Hydrocarbon Contaminated Soils10.1016/j.chemosphere.2012.09.045 ↗
9. Endophytic Bacteria Associated with Hieracium piloselloides: Their Potential for Hydrocarbon-Utilizing and Plant Growth-Promotion10.1080/15287394.2015.1051200 ↗
10. Echinacea purpurea: Pharmacology, Phytochemistry and Analysis Methods10.4103/0973-7847.156353 ↗
11. Physiological Responses and Phytoremediation Ability of Eastern Coneflower (Echinacea purpurea) for Crude Oil Contaminated Soil
12. Phytoremediation as an Approach to Clean up Contaminated Soil, Including Petroleum Product Contamination10.18668/NG.2020.05.05 ↗
13. Pretorius, T.R., Charest, C., Kimpe, L.E., and Blais, J.M. (2018). The Accumulation of Metals, PAHs and Alkyl PAHs in the Roots of Echinacea purpurea. PLoS ONE, 13.10.1371/journal.pone.0208325 ↗
14. Study on the Efficiency of Phytoremediation of Soils Heavily Polluted with PAHs in Petroleum-Contaminated Sites by Microorganism10.1007/s11356-019-05828-1 ↗
15. Sun, C., Shen, X., Zhang, Y., Song, T., Xu, L., and Xiao, J. (2023). Molecular Defensive Mechanism of Echinacea purpurea (L.) Moench against PAH Contaminations. Int. J. Mol. Sci., 24.10.3390/ijms241311020 ↗
16. Defined and Natural PAH Contaminations Shift PAH-Degrading Bacterial Community in Rhizosphere of Ornamental Plant Species Echinacea purpurea L.10.1016/j.eti.2023.103189 ↗
17. Wojtowicz, K., Steliga, T., Kapusta, P., and Brzeszcz, J. (2023). Oil-Contaminated Soil Remediation with Biodegradation by Autochthonous Microorganisms and Phytoremediation by Maize (Zea mays). Molecules, 28.10.3390/molecules28166104 ↗
18. Sui, X., Wang, X., Li, Y., and Ji, H. (2021). Remediation of Petroleum-Contaminated Soils with Microbial and Microbial Combined Methods: Advances, Mechanisms, and Challenges. Sustainability, 13.10.3390/su13169267 ↗
19. Ptaszek, N., Pacwa-Płociniczak, M., Noszczyńska, M., and Płociniczak, T. (2020). Comparative Study on Multiway Enhanced Bio- and Phytoremediation of Aged Petroleum-Contaminated Soil. Agronomy, 10.10.3390/agronomy10070947 ↗
20. Plant-Assisted Remediation of Hydrocarbons in Water and Soil: Application, Mechanisms, Challenges and Opportunities10.1016/j.chemosphere.2020.125932 ↗
21. Hydrocarbon Bioremediation in a Pilot-Scale: A Combination of Bioaugmentation, Phytoremediation, and Vermiremediation10.1016/j.eti.2023.103210 ↗
22. Remediation of PAH-Contaminated Soil Using Achromobacter sp.10.1080/00908319408909078 ↗
23. Hong, Y.-H., Ye, C.-C., Zhou, Q.-Z., Wu, X.-Y., Yuan, J.-P., Peng, J., Deng, H., and Wang, J.-H. (2017). Genome Sequencing Reveals the Potential of Achromobacter sp. HZ01 for Bioremediation. Front. Microbiol., 8.10.3389/fmicb.2017.01507 ↗
24. Biodegradation Potential of Oily Sludge by Pure and Mixed Bacterial Cultures10.1016/j.biortech.2011.09.074 ↗
25. Biodegradation of Phenanthrene Using Adapted Microbial Consortium Isolated from Petrochemical Contaminated Environment10.1016/j.jhazmat.2011.01.034 ↗
26. Zheng, X., Oba, B.T., Shen, C., Rong, L., Zhang, B., Huang, L., Feng, L., Liu, J., Du, T., and Deng, Y. (2023). Effect of the Bacterial Community Assembly Process on the Microbial Remediation of Petroleum Hydrocarbon-Contaminated Soil. Front. Microbiol., 14.10.3389/fmicb.2023.1196610 ↗
27. Wang, D., Lin, J., Lin, J., Wang, W., and Li, S. (2019). Biodegradation of Petroleum Hydrocarbons by Bacillus Subtilis BL-27, a Strain with Weak Hydrophobicity. Molecules, 24.10.3390/molecules24173021 ↗
28. Islam, M.T., Rahman, M., and Pandey, P. (2022). Bacilli in Agrobiotechnology, Springer International Publishing.10.1007/978-3-030-85465-2 ↗
29. Activating Soil Microbial Community Using Bacillus and Rhamnolipid to Remediate TPH Contaminated Soil10.1016/j.chemosphere.2021.130062 ↗
30. Degradation of Petroleum Hydrocarbons (C6–C40) and Crude Oil by a Novel Dietzia Strain10.1016/j.biortech.2011.06.009 ↗
31. High Efficiency Degradation of Alkanes and Crude Oil by a Salt-Tolerant Bacterium Dietzia Species CN-310.1016/j.ibiod.2017.01.029 ↗
32. Biological Process of Alkane Degradation by Gordonia sihwaniensis10.1021/acsomega.1c01708 ↗
33. Kim, H., Dong, K., Kim, J., and Lee, S. (2019). Characteristics of Crude Oil-degrading Bacteria Gordonia iterans Isolated from Marine Coastal in Taean Sediment. MicrobiologyOpen, 8.10.1002/mbo3.754 ↗
34. Deng, Y., Mou, T., Wang, J., Su, J., Yan, Y., and Zhang, Y.-Q. (2023). Characterization of Three Rapidly Growing Novel Mycobacterium Species with Significant Polycyclic Aromatic Hydrocarbon Bioremediation Potential. Front. Microbiol., 14.10.3389/fmicb.2023.1225746 ↗
35. R-Strategist versus K-Strategist for the Application in Bioremediation of Hydrocarbon-Contaminated Soils10.1016/j.ibiod.2015.10.001 ↗
36. Biodegradation of Benzene, Toluene, Ethylbenzene, and o-Xylene by the Bacterium Mycobacterium Cosmeticum Byf-410.1016/j.chemosphere.2012.06.043 ↗
37. Fluoranthene Metabolism in Mycobacterium sp. Strain KR20: Identity of Pathway Intermediates during Degradation and Growth10.1099/00221287-147-10-2783 ↗
38. Characterization of Three Mycobacterium spp. with Potential Use in Bioremediation by Genome Sequencing and Comparative Genomics10.1093/gbe/evv111 ↗
39. A Mycobacterium Strain with Extended Capacities for Degradation of Gasoline Hydrocarbons10.1128/AEM.66.6.2392-2399.2000 ↗
40. Laboratory Scale Bioremediation of Petroleum-Contaminated Soil by Indigenous Microorganisms and Added Pseudomonas aeruginosa Strain Spet10.1016/j.biortech.2010.03.055 ↗
41. Degradation of N-Alkanes and Polycyclic Aromatic Hydrocarbons in Petroleum by a Newly Isolated Pseudomonas aeruginosa DQ810.1016/j.biortech.2010.12.064 ↗
42. Evaluation of Genetic Diversity among Pseudomonas citronellolis Strains Isolated from Oily Sludge-Contaminated Sites10.1128/AEM.69.3.1435-1441.2003 ↗
43. Biodegradation of Pyrene by a Pseudomonas aeruginosa Strain RS1 Isolated from Refinery Sludge10.1016/j.biortech.2014.05.074 ↗
44. Timmis, K.N. (2010). Handbook of Hydrocarbon and Lipid Microbiology, Springer.10.1007/978-3-540-77587-4 ↗
45. Bioremediation of Oily Sludge Polluted Soil Employing a Novel Strain of Pseudomonas aeruginosa and Phytotoxicity of Petroleum Hydrocarbons for Seed Germination10.1016/j.scitotenv.2020.139766 ↗
46. Rhodococcus: A Promising Genus of Actinomycetes for the Bioremediation of Organic and Inorganic Contaminants10.1016/j.jenvman.2022.116220 ↗
47. Bioremediation of the Petroleum Contaminated Desert Steppe Soil with Rhodococcus erythropolis KB1 and Its Effect on the Bacterial Communities of the Soils10.1080/01490451.2021.1964111 ↗
48. Van Hong Thi Pham; Chaudhary, D.K.; Jeong, S.-W.; Kim, J. Oil-Degrading Properties of a Psychrotolerant Bacterial Strain, Rhodococcus sp. Y2-2, in Liquid and Soil Media10.1007/s11274-018-2415-x ↗
49. The Effect of Bioaugmentation of Petroleum-Contaminated Soil with Rhodococcus erythropolis Strains on Removal of Petroleum from Soil10.1016/j.ecoenv.2018.11.081 ↗
50. In Situ Bioremediation of Petroleum Hydrocarbon–Contaminated Soil: Isolation and Application of a Rhodococcus Strain10.1007/s10123-022-00305-1 ↗
51. Improvement of Phytoremediation of an Aged Petroleum Hydrocarbon-Contaminated Soil by Rhodococcus erythropolis CD 106 Strain10.1080/15226514.2016.1278420 ↗
52. Mukherjee, A. (2016). New and Future Developments in Microbial Biotechnology and Bioengineering, Elsevier.
53. Transcriptomic Analysis of Polyaromatic Hydrocarbon Degradation by the Halophilic Fungus Aspergillus sydowii at Hypersaline Conditions10.1111/1462-2920.15166 ↗
54. Transmembrane Transport Mechanism of N-Hexadecane by Candida tropicalis: Kinetic Study and Proteomic Analysis10.1016/j.ecoenv.2020.111789 ↗
55. Rana, S., Handa, S., Aggarwal, Y., Puri, S., and Chatterjee, M. (2023). Role of Candida in the Bioremediation of Pollutants: A Review. Lett. Appl. Microbiol., 76.10.1093/lambio/ovad103 ↗
56. Biodegradation of Anthracene and Several PAHs by the Marine-Derived Fungus Cladosporium sp. CBMAI 123710.1016/j.marpolbul.2017.10.023 ↗
57. Assessing Some Cladosporium Species in the Biodegradation of Petroleum Hydrocarbon for Treating Oil Contamination10.1111/jam.15815 ↗
58. Mycoremediation of Hydrocarbon and Its Products Using Fusarium oxysporum10.5958/0974-360X.2019.00725.X ↗
59. Biodegradation of Anthracene and Benz[a]Anthracene by Two Fusarium Solani Strains Isolated from Mangrove Sediments10.1016/j.biortech.2010.07.049 ↗
60. Zehra, A., Dubey, M.K., Meena, M., Aamir, M., Patel, C.B., and Upadhyay, R.S. (2018). New and Future Developments in Microbial Biotechnology and Bioengineering, Elsevier.
61. Biodegradation of Petrol Using the Fungus Penicillium sp.10.17311/sciintl.2014.26.31 ↗
62. Wojtowicz, K., Steliga, T., Kapusta, P., Brzeszcz, J., and Skalski, T. (2022). Evaluation of the Effectiveness of the Biopreparation in Combination with the Polymer γ-PGA for the Biodegradation of Petroleum Contaminants in Soil. Materials, 15.10.3390/ma15020400 ↗
63. Poly-γ-Glutamic Acid Improves Water-Stable Aggregates, Nitrogen and Phosphorus Uptake Efficiency, Water-Fertilizer Productivity, and Economic Benefit in Barren Desertified Soils of Northwest China10.1016/j.agwat.2020.106551 ↗
64. Zając, E., Fabiańska, M.J., Jędrszczyk, E., and Skalski, T. (2022). Hydrocarbon Degradation and Microbial Survival Improvement in Response to γ-Polyglutamic Acid Application. Int. J. Environ. Res. Public Health, 19.10.3390/ijerph192215066 ↗
65. Steliga, T., Wojtowicz, K., Kapusta, P., and Brzeszcz, J. (2020). Assessment of Biodegradation Efficiency of Polychlorinated Biphenyls (PCBs) and Petroleum Hydrocarbons (TPH) in Soil Using Three Individual Bacterial Strains and Their Mixed Culture. Molecules, 25.10.3390/molecules25030709 ↗
66. Changes in Toxicity during in Situ Bioremediation of Weathered Drill Wastes Contaminated with Petroleum Hydrocarbons10.1016/j.biortech.2012.08.092 ↗
67. Studies on Organic and In-Organic Biostimulants in Bioremediation of Diesel-Contaminated Arable Soil10.1016/j.chemosphere.2016.07.074 ↗
68. Opracowanie Metodyki Oznaczania WWA w Próbkach Gleb z Wykorzystaniem Chromatografii Cieczowej HPLC10.18668/NG.2022.02.06 ↗
69. Zastosowanie Testów Toksykologicznych w Przemyśle Naftowym10.18668/NG.2018.09.07 ↗
70. Chapter 16 Phytoremediation of Hydrocarbon-Contaminated Soils: Principles and Applications10.1016/S0167-2991(04)80157-5 ↗
71. Effects of Low-Molecular-Weight Organic Acids on the Dissolution of Hydroxyapatite Nanoparticles10.1039/C6EN00085A ↗
72. Bacteria Associated with Oak and Ash on a TCE-Contaminated Site: Characterization of Isolates with Potential to Avoid Evapotranspiration of TCE10.1007/s11356-009-0154-0 ↗
73. Bioaugmentation with Engineered Endophytic Bacteria Improves Contaminant Fate in Phytoremediation10.1021/es901997z ↗
74. Phytoremediation: Plant–Endophyte Partnerships Take the Challenge10.1016/j.copbio.2009.02.012 ↗
75. Li, D., Hou, L., Gao, Y., Tian, Z., Fan, B., Wang, F., and Li, S. (2022). Recent Advances in Microbial Synthesis of Poly-γ-Glutamic Acid: A Review. Foods, 11.10.3390/foods11050739 ↗
76. A Review on the Genetics of Aliphatic and Aromatic Hydrocarbon Degradation10.1007/s12010-015-1881-y ↗
77. Inoculation with Bacteria in Floating Treatment Wetlands Positively Modulates the Phytoremediation of Oil Field Wastewater10.1016/j.jhazmat.2018.02.013 ↗
78. Enhanced Clean-up of Lead-Contaminated Alluvial Soil through Chrysanthemum indicum L.10.1007/s13762-013-0488-5 ↗
79. Ojuederie, O., and Babalola, O. (2017). Microbial and Plant-Assisted Bioremediation of Heavy Metal Polluted Environments: A Review. Int. J. Environ. Res. Public Health, 14.10.3390/ijerph14121504 ↗
80. Co-Planted Floating Phyto-Bed along with Microbial Fuel Cell for Enhanced Textile Effluent Treatment10.1016/j.jclepro.2018.08.336 ↗
81. Exploiting Plant–Microbe Partnerships to Improve Biomass Production and Remediation10.1016/j.tibtech.2009.07.006 ↗
82. The Inoculation Method Affects Colonization and Performance of Bacterial Inoculant Strains in the Phytoremediation of Soil Contaminated with Diesel Oil10.1080/15226514.2011.552928 ↗
83. Xu, X., Liu, W., Tian, S., Wang, W., Qi, Q., Jiang, P., Gao, X., Li, F., Li, H., and Yu, H. (2018). Petroleum Hydrocarbon-Degrading Bacteria for the Remediation of Oil Pollution Under Aerobic Conditions: A Perspective Analysis. Front. Microbiol., 9.10.3389/fmicb.2018.02885 ↗
84. Transgenic Plants in Phytoremediation: Recent Advances and New Possibilities10.1021/es051134l ↗
85. An Overview of Phytoremediation as a Potentially Promising Technology for Environmental Pollution Control10.1007/s12257-013-0193-8 ↗
86. Biotechnological Advances in Bioremediation of Heavy Metals Contaminated Ecosystems: An Overview with Special Reference to Phytoremediation10.1007/s13762-013-0299-8 ↗
87. Conjunction of Vetiveria Zizanioides L. and Oil-Degrading Bacteria as a Promising Technique for Remediation of Crude Oil-Contaminated Soils10.1016/j.jclepro.2019.119719 ↗
88. Rohrbacher, F., and St-Arnaud, M. (2016). Root Exudation: The Ecological Driver of Hydrocarbon Rhizoremediation. Agronomy, 6.10.3390/agronomy6010019 ↗
89. Accelerating Phytoextraction of Petroleum Hydrocarbon with Organic Stimulant10.1016/j.mex.2021.101509 ↗
90. Fernández Rodríguez, M.D., García Gómez, M.C., Alonso Blazquez, N., and Tarazona, J.V. (2014). Encyclopedia of Toxicology, Elsevier.
91. Bioremediation of Aged Petroleum Oil Contaminated Soil: From Laboratory Scale to Full Scale Application10.1016/j.proche.2015.03.045 ↗