HPLC Analysis of Polyphenolic Compounds in Lysimachia nummularia L. and Comparative Determination of Antioxidant Capacity
Source abstract
Abstract
Lysimachia nummularia L. is a perennial herbaceous plant rich in bioactive compounds, which can be utilized for medicinal purposes. The present work aims to analyze the phenolic compounds from different parts of the Lysimachia nummularia L. plant using the HPLC technique: Lysimachiae radix (the root part), Lysimachiae herba (the aerial part), Lysimachiae flores (flowers). In order to determine the phenolic compounds, extraction from the three categories of vegetable products was performed with ethanol 70% (v/v) using three extraction methods: (i) Soxhlet extraction, (ii) maceration and (iii) ultrasonic-assisted extraction. The content of polyphenols was determined by the Folin–Ciocalteu method, and the antioxidant activity was evaluated by the DPPH, ABTS, FRAP and CUPRAC methods. The antioxidant activity was correlated with the content of phenolic compounds in the analyzed extracts. The following phenolic compounds were separated, identified and quantified: 3-O-methylgallic, gallic, ferulic, caffeic, chlorogenic, p-coumaric acids and trans-resveratrol. According to the experimental data, the highest content of total polyphenols was observed in the hydroethanolic extract from Lysimachiae flores (22.10 ± 1.48 mg gallic acid/g), which also presented remarkable antioxidant activity.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Phytochemicals and Antioxidant Activities · Antioxidant Activity and Oxidative Stress · Natural Antidiabetic Agents Studies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Analysis and Quality Control of Drugs, Faculty of Pharmacy, “Ovidius” University of Constanta, 900470 Constanta, Romania
Department of Chemistry and Quality Control of Drugs, Faculty of Pharmacy, “Ovidius” University of Constanta, 900470 Constanta, Romania
Department of Mathematics and Informatics, Faculty of Pharmacy, “Ovidius” University of Constanta, 900470 Constanta, Romania
“Ilie Murgulescu” Institute of Physical Chemistry, 060021 Bucharest, Romania
Department of Pharmacognosy, Faculty of Pharmacy, “Ovidius” University of Constanta, 900470 Constanta, Romania
Department of Clinical Laboratory and Food Safety, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, 020956 Bucharest, Romania
Department of Organic Chemistry, Faculty of Pharmacy, “Ovidius” University of Constanta, 900470 Constanta, Romania
Departament of Dental Medicine, Faculty of Dental Medicine, Ovidius University of Constanta, 900684 Constanta, Romania
Department of Oral Rehabilitation, Faculty of Dental Medicine, Ovidius University of Constanta, 900684 Constanta, Romania
Department of Microbiology and Immunology, Faculty of Dental Medicine, Ovidius University of Constanta, 900684 Constanta, Romania
References (49)
Show source-provided references
1. Oxidative stress in periodontitis: A critical link to cardiovascular disease
2. Periodontal Disease-Induced Atherosclerosis and Oxidative Stress10.3390/antiox4030577 ↗
3. A new species of Lysimachia (Myrsinaceae) from Dabieshan Mountain, China10.1007/s00606-014-0986-z ↗
4. Lysimachia Nummularia (Primulaceae) naturalised in New South Wales, Australia10.7751/telopea10779 ↗
5. Polyphenol composition and antioxidant capacity of three Lysimachia species
6. Wu, N.H., Ke, Z.Q., Wu, S., Yang, X.S., Chen, Q.J., Huang, S.T., and Liu, C. (2018). Evaluation of the antioxidant and endothelial protective effects of Lysimachia christinae Hance (Jin Qian Cao) extract fractions. BMC Complement Altern Med., 18.10.1186/s12906-018-2157-1 ↗
7. Evaluation of Lysimachia christinae Hance extracts as anticholecystitis and cholagogic agents in animals10.1016/j.jep.2011.04.029 ↗
8. Lysimachia christinae Hance regresses preestablished cholesterol gallstone in mice10.1016/j.jep.2015.03.031 ↗
9. Comparative phytochemical screening of phenoloids in Lysimachia species
10. An ethanol extract of Lysimachia mauritiana exhibits inhibitory activity agains hepatitis E virus genotype 3 replication10.1007/s12275-017-7477-1 ↗
11. Effect of Lysimachia ramosa (Primulaceae) on helminth parasites: Motility, mortality and scanning electron microscopic observations on surface topography10.1016/j.vetpar.2009.12.024 ↗
12. Antioxidant activities of extracts and fractions from Lysimachia foenum-graecum Hance10.1016/j.biortech.2008.07.021 ↗
13. Qualitative and Quantitative LC Profile of Embelin and Rapanone in Selected Lysimachia Species10.1365/s10337-007-0511-4 ↗
14. Evaluation of antibacterial, antitumor, antioxidant activities and phenolic constituents of field-grown and in vitro-grown Lysimachia vulgaris L.10.21010/ajtcam.v14i2.19 ↗
15. Neuroprotective Activity of Methanolic Extract of Lysimachia christinae against Glutamate Toxicity in HT22 Cell and Its Protective Mechanisms10.1155/2020/5352034 ↗
16. Quantification of Saponins in Different Plant Parts of Lysimachia L. Species by validated HPTLC-Densitometric Method10.1556/JPC.26.2013.3.8 ↗
17. Flavonoids of Hawaiian endemic Lysimachia in honour of professor GH Neil Towers 75th birthday10.1016/S0031-9422(97)00841-8 ↗
18. Comparative polyphenolic content and antioxidant activities of two Romanian Lysimachia species
19. Cholinesterase and Tyrosinase Inhibitory Potential and Antioxidant Capacity of Lysimachia verticillaris L. and Isolation of the Major Compounds10.4274/tjps.galenos.2019.71598 ↗
20. Morosan, E., Mititelu, M., Drăgănescu, D., Lupuliasa, D., Ozon, E.A., Karampelas, O., Gîrd, C.E., Aramă, C., Hovanet, M.V., and Musuc, A.M. (2021). Investigation into the Antioxidant Activity of Standardized Plant Extracts with Pharmaceutical Potential. Appl. Sci., 11.10.3390/app11188685 ↗
21. Morosan, E., Secăreanu, A.A., Musuc, A.M., Mititelu, M., Ioniță, A.C., Ozon, E.A., Dărăban, A.M., and Karampelas, O. (2022). Advances on the Antioxidant Activity of a Phytocomplex Product Containing Berry Extracts from Romanian Spontaneous Flora. Processes, 10.10.3390/pr10040646 ↗
22. Sawicki, T., Starowicz, M., Kłębukowska, L., and Hanus, P. (2022). The Profile of Polyphenolic Compounds, Contents of Total Phenolics and Flavonoids, and Antioxidant and Antimicrobial Properties of Bee Products. Molecules, 27.10.3390/molecules27041301 ↗
23. Evaluation of the chemical composition of Ajuga chamaepitys (L.) schreb. From the spontaneous flora of Romania10.31925/farmacia.2021.3.8 ↗
24. Mititelu, M., Udeanu, D.I., Nedelescu, M., Neacsu, S.M., Nicoara, A.C., Oprea, E., and Ghica, M. (2022). Quality Control of Different Types of Honey and Propolis Collected from Romanian Accredited Bee-Keepers and Consumer’s Risk Assessment. Crystals, 12.10.3390/cryst12010087 ↗
25. Mititelu, M., Moroșan, E., Nicoară, A.C., Secăreanu, A.A., Musuc, A.M., Atkinson, I., Cusu, J.P., Nițulescu, G.M., Ozon, E.A., and Sarbu, I. (2022). Development of immediate release tablets containing calcium lactate synthetized from Black Sea mussel shells. Mar. Drugs, 20.10.3390/md20010045 ↗
26. Analysis of heavy metals and organic pollutants from some Danube river fishes
27. Mititelu, M., Stanciu, G., Drăgănescu, D., Ioniță, A.C., Neacșu, S.M., Dinu, M., Stefanvan Staden, R.-I., and Moroșan, E. (2022). Mussel Shells, a Valuable Calcium Resource for the Pharmaceutical Industry. Mar. Drugs, 20.10.3390/md20010025 ↗
28. Oxidative Stress in Oral Diseases: Understanding Its Relation with Other Systemic Diseases10.3389/fphys.2017.00693 ↗
29. Maximizing total phenolics, total flavonoids contents and antioxidant activity of Moringa oleifera leaf extract by the appropriate extraction method10.1016/j.indcrop.2012.09.021 ↗
30. Determination of total phenolic compounds in plant extracts via Folin-Ciocalteu’s method adapted to the usage of digital images. Food Science and Technology
31. Blainski, A., Lopes, G.C., and De Mello, J.C.P. (2013). Application and Analysis of the Folin Ciocalteu Method for the Determination of the Total Phenolic Content from Limonium brasiliense L.. Molecules, 18.10.3390/molecules18066852 ↗
32. Polyphenolic profile and biological activities of the leaves and aerial parts of echinocystis lobata (michx.) torr. et a. gray (cucurbitaceae)
33. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic
34. Methodological aspects about in vitro evaluation of antioxidant properties10.1016/j.aca.2008.02.047 ↗
35. The ferric reducing ability of plasma (FRAP) as a measure of “Antioxidant power”: The FRAP assay10.1006/abio.1996.0292 ↗
36. High-Performance Liquid Chromatography–Diode Array Detection (HPLC-DAD) Method for the Determination of Phenolic Compounds of Water Chesnut (Trapa natans L.)10.1080/00032719.2022.2048304 ↗
37. Evaluation of Phenolic Compounds in Tilia rubra Subsp. caucasica by HPLCUV and HPLC-UV-MS/MS10.1080/10942912.2011.631252 ↗
38. Determination of polyphenolic compounds of Lysimachia nummularia L.10.32008/GEOLINKS2021/B1/V3/08 ↗
39. Total antioxidant capacity of the Korean diet10.4162/nrp.2014.8.2.183 ↗
40. The relationship between antioxidant activity, first electrochemical oxidation potential, and spin population of flavonoid radicals
41. Antioxidant Activity/Capacity Measurement. 1. Classification, Physicochemical Principles, Mechanisms, and Electron Transfer (ET)-Based Assays10.1021/acs.jafc.5b04739 ↗
42. Superoxide radical- and peroxynitrite-scavenging activity of anthocyanins; structure-activity relationship and their synergism10.1080/10715760600815322 ↗
43. Csepregi, K., Neugart, S., Schreiner, M., and Hideg, E. (2016). Comparative Evaluation of Total Antioxidant Capacities of Plant Polyphenols. Molecules, 21.10.3390/molecules21020208 ↗
44. Antiproliferative, antiandrogenic and cytotoxic effects of novel caffeic acid derivatives in LNCaP human androgen-dependent prostate cancer cells10.1016/j.bmc.2013.08.057 ↗
45. The effects of caffeic, coumaric and ferulic acids on proliferation, superoxide production, adhesion and migration of human tumor cells in vitro10.1016/j.ejphar.2015.09.044 ↗
46. Stage-dependent modulation of limb regeneration by caffeic acid phenethyl ester (CAPE)--immunocytochemical evidence of a CAPE-evoked delay in mesenchyme formation and limb regeneration10.1002/jez.a.20064 ↗
47. Hydrolyzable tannins from hydroalcoholic extract from Poincianella pluviosa stem bark and its wound-healing properties: Phytochemical investigations and influence on in vitro cell physiology of human keratinocytes and dermal fibroblasts10.1016/j.fitote.2014.10.007 ↗
48. Anti-obesity effects of Lysimachia foenum-graecum characterized by decreased adipogenesis and regulated lipid metabolism10.3858/emm.2011.43.4.025 ↗
49. Mongioì, L.M., La Vignera, S., Cannarella, R., Cimino, L., Compagnone, M., Condorelli, R.A., and Calogero, A.E. (2021). The Role of Resveratrol Administration in Human Obesity. Int. J. Mol. Sci., 22.10.3390/ijms22094362 ↗