journal article·2024-07-18Open access

Development and Characterization of Hybrid Burgers Made from Pork and Multi-Ingredient Plant Mixtures and Protected with Lactic Acid Bacteria

Krzysztof Dasiewicz, Iwona Szymanska, Dominika Opat, Elzbieta Hac-Szymanczuk

Applied Sciences· Volume 14· Issue 14

Abstract

Hybrid (meat–plant) products can be a sustainable response to the increased interest in flexitarianism. Their development requires designing appropriate composition and functional properties and ensuring microbiological safety. This study aimed to determine the influence of using various multi-ingredient plant mixtures and pork in different proportions and two recipe variants on the characteristics of baked burgers. Additionally, the impact of lactic acid bacteria (LAB) application on the microbiological quality of raw hybrid/plant burgers was assessed. All products were analyzed in terms of basic chemical composition (NIR spectroscopy) and physicochemical and textural properties (instrumental methods). The raw plant and hybrid burgers met the microbiological requirements. The baked burgers did not significantly differ in terms of their chemical composition, except for the fiber found in plant/hybrid samples. Moreover, the reformulation of burgers had no effect on their cooking yield or water activity. As the content of plant parts increased, the baked burgers were darker (lower L*) and had a more tender texture (lower shear force and penetration force). Overall, the multi-ingredient plant mixtures showed great potential in the development of high-quality hybrid burgers. However, the texture formed can, importantly, determine final consumer acceptance. Therefore, research should be continued, especially in terms of comprehensive textural and sensory analyses.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Agriculture Sustainability and Environmental Impact · Meat and Animal Product Quality · Food Waste Reduction and Sustainability

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Food Technology and Assessment, Institute of Food Science, Warsaw University of Life Sciences—SGGW, 159C Nowoursynowska Street, 02-776 Warsaw, Poland

Department of Food Biotechnology and Microbiology, Institute of Food Sciences, Warsaw University of Life Sciences—SGGW, 159C Nowoursynowska Street, 02-776 Warsaw, Poland

Funding

Food and Nutrition Centre—modernisation of the WULS campus to create a Food and Nutrition Research and Development Centre (CŻiŻ) · European Union from the European Regional Development Fund under the Regional Operational Programme of the Mazowieckie Voivodeship for 2014–2020 · Warsaw University of Life Sciences─SGGW

References (83)

Show source-provided references
1. Meat consumption, health, and the environment10.1126/science.aam5324 ↗
2. Drivers and Barriers Toward Reducing Meat Consumption10.1016/j.appet.2020.104636 ↗
3. Effect of information on consumers’ sensory evaluation of beef, plant-based and hybrid beef burgers10.1016/j.foodqual.2021.104417 ↗
4. Buffering capacity of wet texturized plant proteins in comparison to pork meat10.1016/j.foodres.2021.110803 ↗
5. The best of both worlds? challenges and opportunities in the development of hybrid meat products from the last 3 years10.1016/j.lwt.2022.114235 ↗
6. Grasso, S., and Jaworska, S. (2020). Part meat and part plant: Are hybrid meat products fad or future?. Foods, 9.10.3390/foods9121888 ↗
7. Consumer perception and behaviour regarding sustainable protein consumption: A systematic review10.1016/j.tifs.2016.12.006 ↗
8. Food Futures: Flexitarianism and Sustainability
9. Convenience food products: Are they in line with current consumer trends?
10. Bruner, G.C., Hensel, P.J., and James, K.E. (2010). Marketing Scales Handbook: A Compilation of Multi-Item Measures for Consumer Behavior & Advertising Research, Gower Publishing, Ltd.
11. Reframing convenience food10.1016/j.appet.2015.11.032 ↗
12. Safety and quality of meat products through the use of plant-based ingredients
13. Consumer perception and sensory characteristics of hybrid meat products
14. Meat products with plant-based ingredients: Nutritional and safety aspects
15. improvement of the sensory characteristics and shelf life of hybrid sausages by incorporating plant-based ingredients
16. Emerging bacterial pathogens in meat and poultry: An overview10.1007/s11947-009-0189-8 ↗
17. Gastrointestinal microbial ecology and the safety of our food supply as related to Salmonella
18. Probiotics and prebiotics in animal feeding for safe food production10.1016/j.ijfoodmicro.2010.02.031 ↗
19. Probiotic Lactic Acid Bacteria: A review
20. Recent advances in the use of Lactic Acid Bacteria in meat and meat products
21. Novel meat analogues: Assessing the sensory and physicochemical properties of 3D-printed plant-based meat
22. Composition and physicochemical properties of hybrid meat-plant food products
23. Structure, Texture, and Sensory Properties of Plant-Meat Hybrids Produced by High-Moisture Extrusion10.1016/j.lwt.2022.114345 ↗
24. (2022). Microbiology of the Food Chain. Horizontal Method for the Enumeration of Microorganisms. Part 1: Colony Count at 30 Degrees C by the Pour Plate Technique. Standard No. PN-EN ISO Standard 4833-1:2013-12.
25. (2013). Microbiology of Food and Animal Feeding Stuffs. Horizontal Method for the Enumeration of Mesophilic Lactic Acid Bacteria—Colony Count at 30 Degrees C by the Pour Plate Technique. Standard No. PN-ISO 15214:2002.
26. (2017). Microbiology of Food and Animal Feeding Stuffs. Preparation of Test Samples, Initial Suspension and Decimal Dilutions for Microbiological Examination. Part 2: Specific Rules for the Preparation of Meat and Meat Products. Standard No. PN-EN ISO Standard 6887-2:2017.
27. (2010). Meat and Meat Products—Determination of Fat, Protein and Water Content—Near Infrared Transmission Spectrometry (NIT) Method Using Calibration on Artificial Neural Networks (ANN). Standard No. PN-A-82109:2010.
28. Impact of cooking method on properties of beef and plant-based burgers: Appearance, texture, thermal properties, and shrinkage
29. (2013). Meat and Meat Products—Measurement of pH—Reference Method. Standard No. ISO 2917:2001.
30. Color Difference ∆E—A Survey
31. Sangeeta comparative antioxidant capacity of plant leaves and herbs with their antioxidative potential in meat system under accelerated oxidation conditions10.1007/s11694-020-00571-5 ↗
32. The influence of type of thermal treatment on changes in quality of pates from desinewed poultry meat during storage
33. The effect of selected fiber preparations the quality of pork burgers
34. Tabachnick, B.G., and Fidell, L.S. (2007). Experimental Designs Using ANOVA, Duxbury Press. [1st ed.].
35. Rabiej, M. (2012). Statistics with Statistica Software, Helion Publisher House.
36. Kongo, J.M. (2013). The Role of lactic acid bacteria in safety and flavour development of meat and meat products. Lactic Acid Bacteria—R & D for Food, Health and Livestock Purposes, IntechOpen. Available online: https://www.intechopen.com/chapters/42316.
37. Application of nontraditional meat starter cultures in production of Hungarian salami10.1016/S0168-1605(02)00002-8 ↗
38. Characteristics of microorganisms enter into composition of starter cultures and their application in meat processing
39. Effect of the competitive growth of Lactobacillus sakei MN on the growth kinetics of Listeria monocytogenes Scott A in model meat gravy10.1016/j.foodcont.2015.11.025 ↗
40. Fischer, S.W., and Titgemeyer, F. (2023). Protective Cultures in Food Products: From Science to Market. Foods, 12.10.3390/foods12071541 ↗
41. International Commission on Microbiological Specifications for Foods (ICMSF) (2007). Microorganisms in Foods 8. Use of Data for Assessing Process Control and Product Acceptance, Springer.
42. Meat Nutritional Composition and Nutritive Role in the Human Diet10.1016/j.meatsci.2012.09.018 ↗
43. The usage of vegetables as a source of sweetness in the production of cakes with limited addition of sucrose®
44. The genetic diversity and nutritional quality of proso millet (Panicum miliaceum) and its philippine ecotype, the ancient grain “kabog millet”: A review
45. Meat and plant-based meat analogs: Nutritional profile and in vitro digestion comparison10.1016/j.foodhyd.2023.108886 ↗
46. Kołodziejczak, K., Onopiuk, A., Szpicer, A., and Poltorak, A. (2022). Meat Analogues in the Perspective of Recent Scientific Research: A Review. Foods, 11.10.3390/foods11010105 ↗
47. An investigation of the formulation and nutritional composition of modern meat analogue products10.1016/j.fshw.2019.11.006 ↗
48. (2024, June 05). Regulation (EC) No 1924/2006 of the European Parliament and of the Council of 20 December 2006 on Nutrition and Health Claims Made on Foods. Available online: https://eur-lex.europa.eu/legal-content/en/ALL/?uri=CELEX%3A32006R1924.
49. Novel trends in development of dietary fiber rich meat products—A critical review10.1007/s13197-013-1010-2 ↗
50. Reducing sodium chloride content in meat burgers by adding potassium chloride and onion10.1016/j.profoo.2015.09.047 ↗
51. Are novel plant-based meat alternatives the healthier choice?10.1016/j.foodres.2024.114184 ↗
52. (2023). WHO Global Report on Sodium Intake Reduction, World Health Organization.
53. Detailed Characterization of Plant-Based Burgers10.1038/s41598-021-81684-9 ↗
54. Combined heat transfer and kinetic models to predict cooking loss during heat treatment of beef meat10.1016/j.meatsci.2013.04.061 ↗
55. Establishing temperature and time factors for the post pasteurization of gourmet meat products
56. Barbosa-Cánovas, G.V., Fontana, A.J., Schmidt, S.J., and Labuza, T.P. (2020). Effects of water activity (aw) on microbial stability as a hurdle in food preservation. Water Activity in Foods: Fundamentals and Applications, Wiley. [2nd ed.].10.1002/9781118765982 ↗
57. Quality and sensory attributes of burger formulated from fresh beef cuts (longissmus dorsi) infused with citric acid
58. Botella-Martínez, C., Muñoz-Tebar, N., Lucas-González, R., Pérez-Álvarez, J.A., Fernández-López, J., and Viuda-Martos, M. (2023). Assessment of chemical, physico-chemical and sensory properties of low-sodium beef burgers formulated with flours from different mushroom types. Foods, 12.10.3390/foods12193591 ↗
59. Food Authority NSW Goverment (2024, June 10). Plant Based Alternative Products Survey, Available online: https://www.foodauthority.nsw.gov.au/sites/default/files/2021-03/FI371%202101_Plant%20based%20alternative%20survey%20final.pdf.
60. Microbiological status of vegan ground meat products from German retail10.1007/s00003-023-01461-w ↗
61. Effect of Duration and Drying Temperature on Characteristics of Dried Tomato (Lycopersicon esculentum L.) Cochoro Variety10.1515/aucft-2017-0005 ↗
62. Ardila, P., Honrado, A., Marquina, P., Beltrán, J.A., and Calanche, J.B. (2023). Innovative plant-based burger enriched with Tenebrio molitor meal: Characterization and shelf-life. Foods, 12.10.20944/preprints202308.0295.v1 ↗
63. Tóth, A.J., Dunay, A., Battay, M., Illés, C.B., Bittsánszky, A., and Süth, M. (2021). Microbial spoilage of plant-based meat analogues. Appl. Sci., 11.10.3390/app11188309 ↗
64. Souza, L.V., Martins, E., Moreira, I.M.F.B., and De Carvalho, A.F. (2022). Strategies for the development of bioprotective cultures in food preservation. Int. J. Microbiol., 2022.10.1155/2022/6264170 ↗
65. CIELAB color paths during meat shelf life10.1016/j.meatsci.2019.107889 ↗
66. Color measurement of food products using CIE L* a* b* and RGB color space
67. Modelling the Transport Phenomena and Texture Changes of Chicken Breast Meat during the Roasting in a Convective Oven10.1016/j.jfoodeng.2018.05.021 ↗
68. Influence of heat-induced changes in meat proteins on the quality characteristics of the finished product10.1088/1755-1315/848/1/012050 ↗
69. Physical, chemical, and sensory characteristics of chicken sausage with analog meat substitution10.21776/ub.industria.2021.010.01.8 ↗
70. Comparison of the Quality of Selected Meat Products and Their Plant-Based Analogs10.31883/pjfns/162504 ↗
71. Effect of Changes in the Structure of Myoglobin on the Color of Meat Products
72. Carrot (Daucus carota L.) peroxidase inactivation, phenolic content and physical changes kinetics due to blanching10.1016/j.jfoodeng.2009.12.005 ↗
73. Effects of Different Cooking Treatments on the Sensory Qualities and Pigmented Phytochemicals of Carrots10.1016/j.foodchem.2022.135015 ↗
74. Cooking quality, color, and texture profile analysis of a quinoa and lentil pasta10.1590/1413-7054202448015623 ↗
75. Eskin, N.A.M., and Shahidi, F. (2013). Browning Reactions in Foods. Biochemistry of Foods, Elsevier. [3rd ed.].10.1016/B978-0-08-091809-9.00006-6 ↗
76. Jeantet, R., Croguennec, T., Schuck, P., and Brulé, G. (2016). Non-Enzymatic Browning. Handbook of Food Science and Technology 1: Food Alteration and Food Quality, Wiley. [1st ed.].10.1002/9781119268659 ↗
77. Nonenzymatic Browning Reactions: Overview
78. The analysis of correlation between texture parameters of chosen meat products and their chemical components depending on applied measuring probe
79. Structuring processes for meat analogues10.1016/j.tifs.2018.08.011 ↗
80. Texture methods for evaluating meat and meat analogue structures: A review10.1016/j.foodcont.2021.108103 ↗
81. Partial and Total Replacement of Meat by Plant-Based Proteins in Chicken Sausage: Evaluation of Mechanical, Physico-Chemical and Sensory Characteristics10.1007/s13197-019-03754-1 ↗
82. Qualitative improvement of low meat beef burger using Aloe vera10.1016/j.meatsci.2014.09.002 ↗
83. Effect of Binding Agents on Quality Characteristics of Mushroom Based Sausage Analogue10.1111/jfpp.13134 ↗