A Novel Bio-Fertilizer Produced by Prickly Ash Seeds with Biochar Addition Induces Soil Suppressiveness against Black Shank Disease on Tobacco
Source abstract
Abstract
A novel bio-fertilizer, produced from prickly ash seeds (PAS), Bacillus subtilis and biochar, was evaluated for its disease-preventing potential on tobacco black shank caused by Phytophthora nicotianae. The results showed that biochar promoted the growth of Tpb55 in PAS and increased the pH of the organic fertilizer. The final concentration of B. subtilis could reach 1.7 × 1010 cfu g−1 in the biological organic fertilizer (PBB) under the optimal medium under conditions of solid-state fermentation. PBB exhibited a strong fumigation effect on P. nicotianae, including inhibiting mycelium growth, reducing the disease severity and decreasing the pathogen population in rhizospheric soil. PBB treatment also could significantly increase the pH of acidified soil and improve soil nutrition content such as available K, alkali hydrolysable N and organic carbon. High-throughput pyrosequencing of 16S and 18S rRNA genes revealed that 4% PBB addition in soil had significant effects on the diversity and richness of fungi but not on that of bacteria. The microbial community structure was also shifted after PBB treatment. Some potentially beneficial microbes such as Bacillus, Mucor, Cunninghamella, Chitinophaga and Phenylobacterium were enriched, while potential pathogen Fusarium was significantly decreased. In conclusion, the agricultural waste PAS combined with biochar can replace soybean as a source for the production of biocontrol B. subtilis Tpb55, and the novel bio-fertilizer could effectively control tobacco black shank by pathogen inhibition, soil nutrient improvement and shifting the rhizomicrobial community.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Plant Pathogens and Resistance · Plant-Microbe Interactions and Immunity · Plant Pathogens and Fungal Diseases
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China
Sinochem Agriculture Holdings, Beijing 100031, China
Biological Organic Fertilizer Engineering Technology Center of China Tobacco, Zunyi Branch of Guizhou Tobacco Company, Zunyi 563100, China
College of Life, Yangtze University, Jingzhou 434200, China
Funding
China National Tobacco Corporation · Science and Technology Project of Guizhou Tobacco Corporation
References (32)
Show source-provided references
1. A Species-Specific Polymerase Chain Reaction Assay for Rapid Detection of Phytophthora nicotianae in Irrigation Water10.1094/PHYTO.2003.93.7.822 ↗
2. Phytophthora nicotianae diseases worldwide: New knowledge of a long-recognised pathogen
3. Race distribution and distribution of sensitivities to mefenoxam among isolates of Phytophthora parasitica var. nicotianae in Guizhou province of China10.1016/j.cropro.2013.05.020 ↗
4. Wang, Y., Liu, M., Han, X., Zheng, Y., Chao, J., and Zhang, C.-S. (2020). Prickly Ash Seed Kernel: A New Bio-Fumigation Material Against Tobacco Black Shank. Agronomy, 10.10.3390/agronomy10060770 ↗
5. Control of Southern Root-knot Nematodes on Tomato and Regulation of Soil Bacterial Community by Biofumigation with Zanthoxylum bungeanum Seed10.1016/j.hpj.2020.12.005 ↗
6. The role of biochar in organic waste composting and soil improvement: A review10.1016/j.wasman.2019.12.003 ↗
7. Yang, Y., Awasthi, M.K., Bao, H., Bie, J., Lei, S., and Lv, J. (2020). Exploring the microbial mechanisms of organic matter transformation during pig manure composting amended with bean dregs and biochar. Bioresour. Technol., 313.10.1016/j.biortech.2020.123647 ↗
8. Mosharrof, M., Uddin, K., Sulaiman, M., Mia, S., Shamsuzzaman, S., and Haque, A. (2021). Combined Application of Biochar and Lime Increases Maize Yield and Accelerates Carbon Loss from an Acidic Soil. Agronomy, 11.10.3390/agronomy11071313 ↗
9. Immobilization and deactivation of pathogenic enzymes and toxic metabolites by biochar: A possible mechanism involved in soilborne disease suppression10.1016/j.soilbio.2018.03.001 ↗
10. Bio-fertilizer application induces soil suppressiveness against Fusarium wilt disease by reshaping the soil microbiome10.1016/j.soilbio.2017.07.016 ↗
11. Bacillus amyloliquefaciens Strain W19 can Promote Growth and Yield and Suppress Fusarium Wilt in Banana under Greenhouse and Field Conditions10.1016/S1002-0160(15)60083-2 ↗
12. Bao, S. (2000). Soil Agrochemical Analysis, China Agriculture Press Co., Ltd.
13. Integrated control of tobacco black shank by combined use of riboflavin and Bacillus subtilis strain Tpb5510.1007/s10526-017-9849-1 ↗
14. Tobacco bacterial wilt can be biologically controlled by the application of antagonistic strains in combination with organic fertilizer10.1007/s00374-012-0740-z ↗
15. Application of bio-organic fertilizer can control Fusarium wilt of cucumber plants by regulating microbial community of rhizosphere soil10.1007/s00374-012-0675-4 ↗
16. The rhizosphere microbial community response to a bio-organic fertilizer: Finding the mechanisms behind the suppression of watermelon Fusarium wilt disease10.1007/s11738-017-2581-8 ↗
17. Recent developments in biochar utilization as an additive in organic solid waste composting: A review10.1016/j.biortech.2017.07.090 ↗
18. Trichoderma harzianum strain SQR-T37 and its bio-organic fertilizer could control Rhizoctonia solani damping-off disease in cucumber seedlings mainly by the mycoparasitism10.1007/s00253-011-3259-6 ↗
19. Control of cotton Verticillium wilt and fungal diversity of rhizosphere soils by bio-organic fertilizer10.1007/s00374-011-0617-6 ↗
20. Gao, C., El-Sawah, A.M., Ali, D.F.I., Hamoud, Y.A., Shaghaleh, H., and Sheteiwy, M.S. (2020). The Integration of Bio and Organic Fertilizers Improve Plant Growth, Grain Yield, Quality and Metabolism of Hybrid Maize (Zea mays L.). Agronomy, 10.10.3390/agronomy10030319 ↗
21. Hussain, A., Zahir, Z.A., Ditta, A., Tahir, M.U., Ahmad, M., Mumtaz, M.Z., Hayat, K., and Hussain, S. (2020). Production and implication of bio-activated organic fertilizer enriched with zinc-solubilizing bacteria to boost up maize (Zea mays L.) Production and Biofortification under Two Cropping Seasons. Agronomy, 10.10.3390/agronomy10010039 ↗
22. Chen, Y., Xu, Y., Zhou, T., Akkaya, M.S., Wang, L., Li, S., and Li, X. (2020). Biocontrol of Fusarium wilt disease in strawberries using bioorganic fertilizer fortified with Bacillus licheniformis X-1 and Bacillus methylotrophicus Z-1. 3 Biotech, 10.10.1007/s13205-020-2060-6 ↗
23. Hafez, M., Popov, A.I., and Rashad, M. (2021). Integrated use of bio-organic fertilizers for enhancing soil fertility–plant nutrition, germination status and initial growth of corn (Zea mays L.). Environ. Technol. Innov., 21.10.1016/j.eti.2020.101329 ↗
24. Molecular communication in the rhizosphere10.1007/s11104-008-9839-2 ↗
25. Fungal pathogen accumulation at the expense of plant-beneficial fungi as a consequence of consecutive peanut monoculturing10.1016/j.soilbio.2014.01.019 ↗
26. Zheng, X., Zhu, Y., Wang, Z., Zhang, H., Chen, M., Chen, Y., Wang, J., and Liu, B. (2020). Effects of a novel bio-organic fertilizer on the composition of rhizobacterial communities and bacterial wilt outbreak in a continuously mono-cropped tomato field. Appl. Soil Ecol., 156.10.1016/j.apsoil.2020.103717 ↗
27. Shen, Z., Wang, D., Ruan, Y., Xue, C., Zhang, J., Li, R., and Shen, Q. (2014). Deep 16S rRNA Pyrosequencing Reveals a Bacterial Community Associated with Banana Fusarium Wilt Disease Suppression Induced by Bio-Organic Fertilizer Application. PLoS ONE, 9.10.1371/journal.pone.0098420 ↗
28. First record and preliminary evaluation ofMucor hiemalisas biocontrol agent on inflorescence brown rot incidence of date palm10.1080/03235408.2012.749695 ↗
29. Mixed culture fermentation between Rhizopus nigricans and Trichoderma pseudokoningii to control cucumber Fusarium wilt10.1016/j.cropro.2019.104857 ↗
30. A biofertilizer with diazotrophic bacteria and a filamentous fungus increases Pinus pinaster tolerance to the pinewood nematode (Bursaphelenchus xylophilus)10.1016/j.biocontrol.2019.01.013 ↗
31. Biocontrol potential of chitinases produced by newly isolated Chitinophaga sp. S16710.1007/s11274-020-02864-9 ↗
32. Ma, Y., Rajkumar, M., Oliveira, R.S., Zhang, C., and Freitas, H. (2019). Potential of plant beneficial bacteria and arbuscular mycorrhizal fungi in phytoremediation of metal-contaminated saline soils. J. Hazard. Mater., 379.10.1016/j.jhazmat.2019.120813 ↗