journal article·2022-12-17Open access

Effect of Lyophilization Process on Nutritional Value of Meat By-Products

Ignė Juknienė, Gintarė Zaborskienė, Agnė Jankauskienė, Aistė Kabašinskienė, Gintarė Zakarienė, Saulius Bliznikas

Applied Sciences· Volume 12· Issue 24

Abstract

The meat industry generates large amounts of by-products, and their mass represents approximately one-third of the live weight of animals. Most by-products are disposed of and not used for processing, although they meet hygiene requirements and are suitable for human consumption or for the processing of food supplements. The aim of this study was to investigate the effect of lyophilization on the nutritional value and retention of functional ingredients in ovine and porcine by-products: liver, kidneys, hearts, and lungs. For this purpose, meat by-products of the third category were selected in X and Y slaughterhouses and divided into two parts: one part was freeze-dried at −80 °C for 72 h, and the other part was left raw. Fatty acid composition was determined by gas chromatography–mass spectrometry (GC-MS), and amino acid analysis was performed by AccQ Tag technology (Waters Corp., Milford, MA, USA) and HPLC. Our study shows that the lyophilization process did not significantly affect protein and fat content. The largest decrease in the amount of proteins was determined in samples of ovine kidneys, at 0.8%, while the difference in protein in ovine liver samples before and after lyophilization was 0.38%. The composition of essential amino acids did not change after lyophilization, except a decrease in Leu and Thr in porcine samples and Leu, Thr, and Met in ovine samples (p < 0.05). The lyophilization process did not significantly affect the polyunsaturated fatty acid content, including the amounts of omega 3 and omega 6 fatty acids. The optimal ratio of omega 6 and 3 fatty acids was determined in samples of lyophilized ovine livers (2.65), and the largest ratio was found in samples of lyophilized porcine hearts (16.67). The study results show that, after lyophilization, meat by-products of the third category (according to Regulation (EC) No. 1069/2009, Categorization, Article 10), especially ovine liver, can be used as a source of amino acids and polyunsaturated fatty acids for functional food processing. The process of lyophilization is also appropriate for preserving meat by-products without losing the nutritional value and beneficial components.
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Research context

OpenAlex topics: Meat and Animal Product Quality · Protein Hydrolysis and Bioactive Peptides · Food Quality and Safety Studies

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Food Safety and Quality, Veterinary Academy, Lithuanian University of Health Sciences, Mickeviciaus Str. 9, LT-44307 Kaunas, Lithuania

Institute of Animal Rearing Technologies, Faculty of Animal Sciences, Lithuanian University of Health Sciences, Mickeviciaus Str. 9, LT-44307 Kaunas, Lithuania

References (49)

Show source-provided references
1. Innovations in value-addition of edible meat by-products10.1016/j.meatsci.2012.04.004 ↗
2. Hicks, T.M., and Verbeek, C.J.R. (2016). Protein-rich by-products: Production statistics, legislative restrictions, and management options. Protein Byprod., 1–18.10.1016/B978-0-12-802391-4.00001-X ↗
3. Jensen, W.K., Devine, C., and Dikeman, M. (2004). Edible, for Human Consumption. Encyclopedia of Meat Sciences, Elsevier. [2nd ed.].
4. Management of meat by- and co-products for an improved meat processing sustainability10.1016/j.meatsci.2021.108608 ↗
5. New insights into meat by-product utilization10.1016/j.meatsci.2016.04.021 ↗
6. Bioactive peptides from meat muscle and by-products: Generation, functionality and application as functional ingredients10.1016/j.meatsci.2014.05.036 ↗
7. Mora, L., Toldrá-Reig, F., Reig, M., and Toldrá, F. (2019). Bioactive compounds from animal meat byproducts. Byproducts from Agriculture and Fisheries, John Wiley & Sons Ltd.10.1002/9781119383956.ch14 ↗
8. Toldrá, F., Mora, L., and Reig, M. (2022). Current developments in meat by-products. New Aspects of Meat Quality, Elsevier.10.1016/B978-0-323-85879-3.00027-1 ↗
9. Characterization of edible pork by-products by means of yield and nutritional composition10.5851/kosfa.2014.34.3.297 ↗
10. Alao, B.O., Falowo, A.B., Chulayo, A., and Muchenje, V. (2017). The potential of animal by-products in food systems: Production, prospects and challenges. Sustainability, 9.10.3390/su9071089 ↗
11. The Effects of Freeze-Drying Process Parameters on broiler chicken breast meat10.1016/j.lwt.2009.03.020 ↗
12. Characteristics of meat by-products: Nutritional and biological value
13. Effects of drying and grinding in production of fruit and vegetable powders: A Review10.1016/j.jfoodeng.2016.05.001 ↗
14. Drying characteristics of pumpkin (Cucurbita moschata) slices in convective and freeze dryer10.1007/s00231-017-1967-x ↗
15. Effects of lyophilization on colorimetric indices, phenolics content, and antioxidant activity of avocado (Persea americana) pulp10.1016/j.foodcont.2021.108526 ↗
16. Freeze-drying of nanoparticles: Formulation, process and storage considerations10.1016/j.addr.2006.09.017 ↗
17. Utilization of byproducts and waste materials from meat, poultry and fish processing industries: A review10.1007/s13197-011-0290-7 ↗
18. (2022, October 26). Regulation (EC) No 1069/2009 of the European Parliament and of the council and of the Council of 21 October 2009 Laying Down Health Rules as Regards Animal By-Products and Derived Products Not Intended for Human Consumption and Repealing Regulation (EC) No 1774/2002 (Animal By-Products Regulation). Available online: https://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=CELEX:32009R1069&from=EN.
19. (1978). Meat and Meat Products—Determination of Nitrogen Content (Reference Method). Standard No. ISO 937:1978. Available online: https://www.iso.org/standard/5356.html.
20. Spizzirri, U.G., and Cirillo, G. (2017). Current Analytical Techniques for Food Lipids. Food Safety: Innovative Analytical Tools for Safety Assessment, Scrivener Publishing LLC.10.1002/9781119160588 ↗
21. (1997). Meat and Meat Products—Determination of Moisture Content (Reference Method). Standard No. ISO 1442:1997. Available online: https://www.iso.org/standard/6037.html.
22. (2017). Animal and Vegetable Fats and Oils—Gas Chromatography of Fatty Acid Methyl Esters—Part 2: Preparation of Methyl Esters of Fatty Acids. Standard No. ISO 12966-2:2017. Available online: https://www.iso.org/standard/72142.html2-4.
23. (2014). Animal and Vegetable Fats and Oils—Gas Chromatography of Fatty Acid Methyl Esters—Part 1: Guidelines on Modern Gas Chromatography of Fatty Acid Methyl Esters. Standard No. ISO 12966-1:2014. Chapter 7.
24. European Commission (2009). Commission Regulation
25. (EC) No 152/2009 of 27 January 2009 Laying Down the Methods of Sampling and Analysis for the Official Control of Feed. Off. J. Eur. Union, L54, 1.
26. Waters AccQ (1993). Waters AccQ Tag Chemistry Package Instruction Manual, Millipore Corporation.
27. Maysonnave, G.S., de Oliveira Mello, R., Vaz, F.N., de Ávila, M.M., Pascoal, L.L., and Rodrigues, A.C.T. (2020). Physicochemical characterization of by-products from beef cattle slaughter and economic feasibility of commercialization. Acta Sci. Anim. Sci., 42.10.4025/actascianimsci.v42i1.46545 ↗
28. Organ mass and composition in growing dairy goat wethers fed different levels of poultry fat and protein10.1016/j.smallrumres.2011.09.046 ↗
29. Effect of rate of body weight gain of steers during the stocker phase. II. Visceral organ mass and body composition of growing-finishing beef cattle10.2527/jas.2012-5451 ↗
30. Dietary fat and obesity: A review of animal, clinical and epidemiological studies10.1016/j.physbeh.2004.08.039 ↗
31. Physicochemical property analysis of lyophilized fresh, wet-, and dry-aged beef powders: Application of dry-aged beef crust as a food additive10.1016/j.meatsci.2022.109014 ↗
32. Key composition optimization of meat processed protein source by vacuum freeze-drying technology10.1016/j.sjbs.2017.09.013 ↗
33. Wang, J., Du, B., and Yang, G.M. (2010). Flavors of Dried Bananas. Handbook of Fruit and Vegetable Flavors, John Wiley & Sons, Inc.10.1002/9780470622834.ch29 ↗
34. Chen, Y. (2008). Optimization of vacuum freeze-drying technologies of longan fruits. Chinese Soc. Agricult. Eng., 244–248.
35. Liu, D.C. (2002). Better Utilization of By-Products from the Meat Industry, Food and Fertilizer Technology Center.
36. Protein quality of stingray (Himantura gerrardi) fish flakes
37. Changes in curcuminoids and chemical components of turmeric (Curcuma longa L.) under freeze-drying and low-temperature drying methods10.1016/j.foodchem.2020.128121 ↗
38. Protein quality evaluation twenty years after the introduction of the protein digestibility corrected amino acid score method10.1017/S0007114512002309 ↗
39. Influences of hot air drying and microwave drying on nutritional and odorous properties of grass carp (Ctenopharyngodon Idellus) Fillets10.1016/j.foodchem.2008.02.058 ↗
40. Effects of microcapsule constitution on the quality of microencapsulated walnut oil10.1002/ejlt.201100039 ↗
41. Fatty acid contents of lyophilized and frozen Portunus sanguinolentus crab meat10.3923/jfrs.2015.43.48 ↗
42. Beef, chicken and lamb fatty acid analysis—A aimplified direct bimethylation procedure using freeze-dried material10.1016/j.meatsci.2012.06.013 ↗
43. Jiang, G., Ameer, K., Kim, H., Lee, E.J., Ramachandraiah, K., and Hong, G.P. (2020). Strategies for sustainable substitution of livestock meat. Foods, 9.10.3390/foods9091227 ↗
44. Ponnampalam, E.N., Sinclair, A.J., and Holman, B.W.B. (2021). The Sources, synthesis and biological actions of omega-3 and omega-6 fatty acids in red meat: An Overview. Foods, 10.10.3390/foods10061358 ↗
45. Multi-Omic Analysis in transgenic mice implicates omega-6/omega-3 fatty acid imbalance as a risk factor for chronic disease10.1038/s42003-019-0521-4 ↗
46. Overconsumption of omega-6 polyunsaturated fatty acids (PUFAs) versus deficiency of omega-3 PUFAs in modern-day diets: The disturbing factor for their “Balanced antagonistic metabolic functions” in the human body10.1155/2021/8848161 ↗
47. Effect of high ratio of N-6/n-3 PUFAs on depression:A Meta-analysis of prospective studies
48. Simopoulos, A.P. (2016). An increase in the omega-6/omega-3 fatty acid ratio increases the risk for obesity. Nutrients, 8.10.3390/nu8030128 ↗
49. The omega-6/omega-3 fatty acid ratio: Health implications10.1051/ocl.2010.0325 ↗