A Novel Technique Using Confocal Raman Spectroscopy Coupled with PLS-DA to Identify the Types of Sugar in Three Tropical Fruits
Source abstract
Abstract
Tropical fruits such as cherimoya, soursop, and pineapple share sugars (glucose, fructose, and sucrose) in common but may differ in the content of other phytochemicals. In the present work, confocal Raman spectroscopy and partial least squares discriminant analysis (PLS-DA) were used to establish a classification model among the three fruits and to evaluate the effect of pre-processing methods on the model’s performance. The Raman spectra showed that glucose was present in the fruits in the 800–900 cm−1 band and the 1100–1200 cm−1 band. While sucrose was present in the bands of 1131.22 cm−1, 1134.44 cm−1, and 1133.37 cm−1 in the three fruits, fructose was present in the bands of 1464.22 cm−1, 1467.44 cm−1, and 1464.22 cm−1 in cherimoya, soursop, and pineapple. The accuracy of the PLS-DA model varied according to the pre-processing methods used. The Savitzky–Golay first derivative method produced a model with 98.69–100% and 100% precision on the training and prediction data, respectively.
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Research context
OpenAlex topics: Spectroscopy and Chemometric Analyses · Identification and Quantification in Food · Spectroscopy Techniques in Biomedical and Chemical Research
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Affiliations
Instituto de Investigación, Innovación y Desarrollo para el Sector Agrario y Agroindustrial (IIDAA), Facultad de Ingeniería y Ciencias Agrarias, Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas, Calle Higos Urco 342-350-356, Chachapoyas 01000, Peru
Facultad de Ingeniería Zootecnista, Agronegocios y Biotecnología, Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas, Calle Higos Urco 342-350-356, Chachapoyas 01000, Peru
Facultad de Ingeniería Civil y Ambiental, Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas, Calle Higos Urco 342-350-356, Chachapoyas 01000, Peru
Funding
Consejo Nacional de Ciencia y Tecnología e Innovación Tecnológica–Concytec of the Peruvian Government · Vicerrectorado de Investigación, Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas
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