Streptomyces spp. Biofilmed Solid Inoculant Improves Microbial Survival and Plant-Growth Efficiency of Triticum aestivum
Source abstract
Abstract
Streptomyces species have been successfully used in diverse biotechnological processes; however, little is known about actinobacterial biofilm formation and its use as a biofilmed inoculant. The present study assessed and compared the ability of some plant growth-promoting actinobacterial strains to form biofilms on a carrier to improve microbial survival and colonize the rhizosphere and roots of Triticum aestivum, resulting in positive interactions and benefits to the plant. Forty-one actinobacterial isolates from Persea americana organic rhizosphere farms were tested on plant growth and biofilm-forming capacities, showing their potential use as bio-fertilizers in agriculture. Three Streptomyces strains were selected and tested for biofilm formation and plant growth-promoting (PGP) features. Biofilms were induced on the perlite carrier and used to inoculate seven treatments with T. aestivum in pot essays, resulting in a >200% increase in root weight and a >400% increase in total biomass. Endophytic colonization was achieved in all the treatments. Microbial survival ranged between 108 and 109 CFU/g after 12 weeks of treatment, indicating actinobacterial permanence on the carrier. Successful consortia formation was observed for mixed-strain treatments, suggesting long-term plant recolonization.
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Research context
OpenAlex topics: Plant-Microbe Interactions and Immunity · Legume Nitrogen Fixing Symbiosis · Mycorrhizal Fungi and Plant Interactions
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Facultad de Químico Farmacobiología, Universidad Michoacana de San Nicolás de Hidalgo, Tzintzuntzan 173, Col. Matamoros, Morelia 58240, Michoacán, Mexico
Laboratorio de Epidemiología Molecular y Biotecnología de Enfermedades Infecciosas, Centro Multidisciplinario de Estudios en Biotecnología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolás de Hidalgo, Km 9.5 Carretera Morelia-Zinapécuaro s/n, Posta Veterinaria. La Palma, Tarímbaro, C.P., Morelia 58893, Michoacán, Mexico
Escuela Nacional de Estudios Superiores (ENES), Unidad Morelia, UNAM. Antigua Carretera a Pátzcuaro 8701, col. Ex Hacienda San José de la Huerta, Morelia 58190, Michoacán, Mexico
Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio, Calle Plan de Ayala s/n, Santo Tomás, Miguel Hidalgo, Ciudad de México 11350, Mexico
Facultad de Ingeniería Civil, Universidad Michoacana de San Nicolás de Hidalgo, Gral. Francisco J. Mújica 172B, Felicitas del Río, Morelia 58040, Michoacán, Mexico
Funding
Coordinación de la Investigación Científica-UMSNH · PRODEP
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