Probiotic Characteristics of Ligilactobacillus salivarius AS22 Isolated from Sheep Dung and Its Application in Corn-Fox Tail Millet Silage
Source abstract
Abstract
The forage crops corn (Zea mays) and foxtail millet (Panicum italicum L.) are widely used as animal feed because of their high nutritive values. The ensiling of corn and foxtail millet is often associated with the growth of undesirable microbes, which cause severe loss of dry matter content during the storage periods. The selection of suitable Ligilactobacillus species for corn-fox tail millet silage production can improve the quality. In this study, we aimed to select potent lactic acid bacteria (LAB) from sheep dung and analyses their biological application such as probiotic features, antimicrobial activities and fermentation capability of silage. A total of nine Lactobacillus strains were inoculated in MRS medium to evaluate lactic acid concentration. The isolated strain, Ligilactobacillus salivarius AS22, produced a higher lactic acid level (40.2 ± 2.2 µg/mL) with high growth rates (2.24 ± 0.12 OD at 600 nm) compared to other strains. The silage treated with inoculant (L. salivarius AS22) decreased the pH value (p < 0.05) and enhanced lactic acid production (p < 0.05) than the control at ensiled silages. LAB inoculated silage had reduced numbers of fungal colonies than control (p < 0.05). In conclusion, the addition of L. salivarius AS22 improved the quality of whole corn and foxtail millet silages with significant probiotic potential.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Probiotics and Fermented Foods · Food composition and properties · Protein Hydrolysis and Bioactive Peptides
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Food Science and Biotechnology, College of Life Sciences, Sejong University, Seoul 05006, Korea
Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan 31000, Korea
Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
Bioprocess Engineering Division, Smykon Biotech, Nagercoil, Kanyakumari 629201, India
Department of Zoology and Biotechnology, A.V.V.M. Sri Pushpam College (Autonomous), Poondi, Thanjavur 613503, India
Funding
King Saud University
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