The Potential for Restoring the Activity of Oxidoreductases and Hydrolases in Soil Contaminated with Petroleum Products Using Perlite and Dolomite
Source abstract
Abstract
The research focused on assessing the response of oxidoreductases (dehydrogenases and catalase) and hydrolases (urease, acid phosphatase, alkaline phosphatase, arylsulfatase, and β-glucosidase) to diesel oil (DO) and gasoline (G) contamination of soils subjected to phytoremediation with Zea mays. The activity of enzymes constitutes one of the fundamental mechanisms for the removal of contaminants from soil, which have the potential to contaminate not only the soil but also groundwater and water reservoirs. Additionally, correlations between enzyme activity and the basic physicochemical properties of the soil were determined. The interaction of perlite and dolomite with soil enzymes and the cultivated plant was also tested. The study was carried out in a pot experiment, where soil contaminated with DO or G was artificially treated at doses of 0, 8 cm3, and 16 cm3 kg−1. Perlite and dolomite were applied for remediation at doses of 0 and 10 g kg−1 of soil. Zea mays was found to respond to the tested pollutant with a reduction in biomass. DO affected the growth of this plant more than G. DO reduced the yield of aerial parts by 86% and G by 74%. The negative effects of these pollutants on the growth and development of Zea mays were mitigated by both perlite and dolomite. DO exerted greater pressure than G on the activity of oxidoreductases and hydrolases, as well as on the physicochemical properties of the soil. DO enhanced the activity of oxidoreductases and most hydrolases, whereas G inhibited them. The implementation of dolomite intensified the activity of all enzymes, except AcP (acid phosphatase) and Glu (ß-glucosidase), in soil contaminated with DO and G, and also improved its physicochemical properties. Perlite induced less significant effects than dolomite on soil enzymes and the physicochemical properties of the soil.
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Research context
OpenAlex topics: Pesticide and Herbicide Environmental Studies · Microbial bioremediation and biosurfactants · Clay minerals and soil interactions
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Soil Science and Microbiology, Faculty of Agriculture and Forestry, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland
Funding
University of Warmia and Mazury in Olsztyn, Faculty of Agriculture and Forestry, Department of Soil Science and Microbiology · Minister of Education and Science
References (100+)
Show source-provided references
1. Sarma, H., and Joshi, S.J. (2024). Biotechnology of Emerging Microbes, Academic Press. Progress in Biochemistry and Biotechnology.
2. Oil Refinery and Water Pollution in the Context of Sustainable Development: Developing and Developed Countries10.1016/j.jclepro.2021.126987 ↗
3. Bioremediation of Petroleum Hydrocarbon Contaminated Soil: A Review on Principles, Degradation Mechanisms, and Advancements10.3389/fenvs.2024.1354422 ↗
4. Hydrocarbon-Based Parameters for the Identification of Oil Contamination in an Area with Brown Coal Mining: Emphasis on Pyrogenic Index and Alkylated PAHs10.1016/j.envadv.2024.100484 ↗
5. Comprehensive Review on Toxicity of Persistent Organic Pollutants from Petroleum Refinery Waste and Their Degradation by Microorganisms10.1016/j.chemosphere.2017.09.005 ↗
6. Mutual Effects of Crude Oil and Plants in Contaminated Soil: A Field Study10.1007/s10653-021-00973-4 ↗
7. Changes in Bacterial Populations during Bioremediation of Soil Contaminated with Petroleum Hydrocarbons10.1007/s11270-016-2789-z ↗
8. Mtewa, A.G., and Egbuna, C. (2021). Phytochemistry, the Military and Health, Elsevier.
9. Ecological Risk Assessment of Priority PAHs Pollutants in Crude Oil Contaminated Soil and Its Impacts on Soil Biological Properties10.1016/j.jhazmat.2022.129325 ↗
10. Microbe-Assisted Rhizodegradation of Hydrocarbons and Growth Enhancement of Wheat Plants in Hydrocarbons Contaminated Soil10.1007/s13762-023-05174-3 ↗
11. Wyszkowska, J., Borowik, A., Zaborowska, M., and Kucharski, J. (2023). Biochar, Halloysite, and Alginite Improve the Quality of Soil Contaminated with Petroleum Products. Agriculture, 13.10.3390/agriculture13091669 ↗
12. Borowik, A., Wyszkowska, J., Kucharski, M., and Kucharski, J. (2020). The Role of Dactylis Glomerata and Diesel Oil in the Formation of Microbiome and Soil Enzyme Activity. Sensors, 20.10.3390/s20123362 ↗
13. Assessment of Tolerance Limits of Petroleum Residues in Soil Organic Matter: Sorption of Dichlorobenzene by Soil10.1007/s10653-023-01798-z ↗
14. The Influence of Oil Pollutants on the Mechanical Properties of Clayey Sand10.1007/s41742-024-00575-9 ↗
15. Gospodarek, J., Rusin, M., Barczyk, G., and Nadgórska-Socha, A. (2021). The Effect of Petroleum-Derived Substances and Their Bioremediation on Soil Enzymatic Activity and Soil Invertebrates. Agronomy, 11.10.3390/agronomy11010080 ↗
16. Impacts of Rhizoremediation and Biostimulation on Soil Microbial Community, for Enhanced Degradation of Petroleum Hydrocarbons in Crude Oil-Contaminated Agricultural Soils10.1007/s11356-023-29033-3 ↗
17. Volatile Organic Compounds: A Threat to the Environment and Health Hazards to Living Organisms—A Review10.1016/j.jbiotec.2023.12.013 ↗
18. Radice, R.P., De Fabrizio, V., Donadoni, A., Scopa, A., and Martelli, G. (2023). Crude Oil Bioremediation: From Bacteria to Microalgae. Processes, 11.10.3390/pr11020442 ↗
19. Environmental Effects from Petroleum Product Transportation Spillage in Nigeria: A Critical Review10.1007/s11356-023-31117-z ↗
20. Tracking the Behavior of an Accidental Oil Spill and Its Impacts on the Marine Environment in the Eastern Mediterranean10.1016/j.marpolbul.2023.115887 ↗
21. Borowik, A., Wyszkowska, J., Kucharski, M., and Kucharski, J. (2019). Implications of Soil Pollution with Diesel Oil and BP Petroleum with ACTIVE Technology for Soil Health. Int. J. Environ. Res. Public Health, 16.10.3390/ijerph16142474 ↗
22. Shelyakin, P.V., Semenkov, I.N., Tutukina, M.N., Nikolaeva, D.D., Sharapova, A.V., Sarana, Y.V., Lednev, S.A., Smolenkov, A.D., Gelfand, M.S., and Krechetov, P.P. (2022). The Influence of Kerosene on Microbiomes of Diverse Soils. Life, 12.10.3390/life12020221 ↗
23. Nebeská, D., Auer Malinská, H., Erol, A., Pidlisnyuk, V., Kuráň, P., Medžová, A., Smaha, M., and Trögl, J. (2021). Stress Response of Miscanthus Plants and Soil Microbial Communities: A Case Study in Metals and Hydrocarbons Contaminated Soils. Appl. Sci., 11.10.3390/app11041866 ↗
24. Ali, M.H., Khan, M.I., Naveed, M., and Tanvir, M.A. (2023). Microbe-Assisted Rhizoremediation of Hydrocarbons and Growth Promotion of Chickpea Plants in Petroleum Hydrocarbons-Contaminated Soil. Sustainability, 15.10.3390/su15076081 ↗
25. Borowik, A., Wyszkowska, J., and Kucharski, J. (2021). Microbiological Study in Petrol-Spiked Soil. Molecules, 26.10.3390/molecules26092664 ↗
26. Dubey, S.K., Jha, P.K., Gupta, P.K., Nanda, A., and Gupta, V. (2022). Soil-Water, Agriculture, and Climate Change: Exploring Linkages, Water Science and Technology Library; Springer International Publishing.10.1007/978-3-031-12059-6 ↗
27. Bioremediation Assessment, Hematological, and Biochemical Responses of the Earthworm (Allolobophora Caliginosa) in Soil Contaminated with Crude Oil10.1007/s11356-021-13889-4 ↗
28. Minnikova, T., Kolesnikov, S., Revina, S., Ruseva, A., and Gaivoronsky, V. (2023). Enzymatic Assessment of the State of Oil-Contaminated Soils in the South of Russia after Bioremediation. Toxics, 11.10.3390/toxics11040355 ↗
29. Bio-Augmentation and Bio-Stimulation with Kenaf Core Enhanced Bacterial Enzyme Activities during Bio-Degradation of Petroleum Hydrocarbon in Polluted Soil10.1038/s41598-023-50882-y ↗
30. Zhang, J., Jin, K., Luo, Y., Du, L., Tian, R., Wang, S., Shen, Y., Zhang, J., Li, N., and Shao, W. (2023). Responses of Soil Enzyme Activity to Long-Term Nitrogen Enrichment and Water Addition in a Typical Steppe. Agronomy, 13.10.3390/agronomy13071920 ↗
31. Enzyme Activities as Soil Health Indicators in Relation to Soil Characteristics and Crop Production10.1002/agg2.20297 ↗
32. Milosavljevic, J.S., Serbula, S.M., Cokesa, D.M., Milanovic, D.B., Radojevic, A.A., Kalinovic, T.S., and Kalinovic, J.V. (2020). Soil Enzyme Activities under the Impact of Long-Term Pollution from Mining-Metallurgical Copper Production. Eur. J. Soil Biol., 101.10.1016/j.ejsobi.2020.103232 ↗
33. Soil Enzyme Activities and Enzyme Activity Indices in Long-Term Arsenic-Contaminated Soils10.1134/S106422932210012X ↗
34. Lee, S.H., Kim, M.S., Kim, J.G., and Kim, S.O. (2020). Use of Soil Enzymes as Indicators for Contaminated Soil Monitoring and Sustainable Management. Sustainability, 12.10.3390/su12198209 ↗
35. A Proposal of “Core Enzyme” Bioindicator in Long-Term Pb-Zn Ore Pollution Areas Based on Topsoil Property Analysis10.1016/j.envpol.2016.03.030 ↗
36. Long-Term Effects of Oil Contamination on Soil Quality and Metabolic Function10.1007/s10653-023-01779-2 ↗
37. Daunoras, J., Kačergius, A., and Gudiukaitė, R. (2024). Role of Soil Microbiota Enzymes in Soil Health and Activity Changes Depending on Climate Change and the Type of Soil Ecosystem. Biology, 13.10.3390/biology13020085 ↗
38. The Biological Activities of β-Glucosidase, Phosphatase and Urease as Soil Quality Indicators: A Review10.4067/S0718-95162017000300018 ↗
39. Li, J., Wu, J., Yu, J., Wang, K., Li, J., Cui, Y., Shangguan, Z., and Deng, L. (2024). Soil Enzyme Activity and Stoichiometry in Response to Precipitation Changes in Terrestrial Ecosystems. Soil Biol. Biochem., 191.10.1016/j.soilbio.2024.109321 ↗
40. Altered Activities of Extracellular Soil Enzymes by the Interacting Global Environmental Changes10.1111/gcb.16604 ↗
41. Soil Conservation Service Underpins Sustainable Development Goals
42. Hidalgo, K.J., Giachini, A.J., Schneider, M.R., Soriano, A.U., Baessa, M.P., Martins, L.F., and Oliveira, V.M. (2024). Recent Advances in Bioremediation of Biofuel Blends. Int. Biodeterior. Biodegrad., 188.10.1016/j.ibiod.2024.105750 ↗
43. An Evaluation Model for In-Situ Bioremediation Technology of Petroleum Hydrocarbon Contaminated Soil10.1016/j.envpol.2024.123299 ↗
44. The Role of Microorganisms in Petroleum Degradation: Current Development and Prospects10.1016/j.scitotenv.2022.161112 ↗
45. Effects of Biochar Properties on the Bioremediation of the Petroleum-Contaminated Soil from a Shale-Gas Field10.1007/s11356-020-09715-y ↗
46. Biochar-Based Materials as Remediation Strategy in Petroleum Hydrocarbon-Contaminated Soil and Water: Performances, Mechanisms, and Environmental Impact10.1016/j.jes.2023.04.008 ↗
47. Combined Impacts of Bioaugmentation and Vermiremediation on Crude Oil-Contaminated Soil: Mitigating Strategies for Prospective Environmental Management10.1016/j.emcon.2024.100302 ↗
48. A Systematic Review on the Effectiveness of Remediation Methods for Oil Contaminated Soils10.1016/j.envadv.2022.100319 ↗
49. Use of Natural Sorbents for Accelerated Bioremediation of Grey Forest Soil Contaminated with Crude Oil10.1016/j.scitotenv.2022.157952 ↗
50. Evolution in Mitigation Approaches for Petroleum Oil-Polluted Environment: Recent Advances and Future Directions10.1007/s11356-021-16047-y ↗
51. Biochar as a Sustainable Product for Remediation of Petroleum Contaminated Soil10.1016/j.crgsc.2021.100055 ↗
52. Wyszkowski, M., Wyszkowska, J., Kordala, N., and Zaborowska, M. (2023). Molecular Sieve, Halloysite, Sepiolite and Expanded Clay as a Tool in Reducing the Content of Trace Elements in Helianthus annuus L. on Copper-Contaminated Soil. Materials, 16.10.3390/ma16051827 ↗
53. Wyszkowska, J., Borowik, A., Zaborowska, M., and Kucharski, J. (2022). Evaluation of the Usefulness of Sorbents in the Remediation of Soil Exposed to the Pressure of Cadmium and Cobalt. Materials, 15.10.3390/ma15165738 ↗
54. Simultaneous Realization of Cadmium Bioavailability Reduction and Spinach Growth Promotion in Acidic Soils with Calcined Oyster Shells and Dolomite10.1111/sum.12979 ↗
55. Wyszkowska, J., Borowik, A., Zaborowska, M., and Kucharski, J. (2023). The Usability of Sorbents in Restoring Enzymatic Activity in Soils Polluted with Petroleum-Derived Products. Materials, 16.10.3390/ma16103738 ↗
56. Influence of Perlite/Biosolid Composition on Growth and Uptake of Cd and Mn by Radish (Raphanus Sativus L.) under Greenhouse Conditions10.1007/s13201-023-02059-1 ↗
57. Kordala, N., and Wyszkowski, M. (2024). Zeolite Properties, Methods of Synthesis, and Selected Applications. Molecules, 29.10.3390/molecules29051069 ↗
58. Boros-Lajszner, E., Wyszkowska, J., and Kucharski, J. (2020). Use of a Zeolite and Molecular Sieve to Restore Homeostasis of Soil Contaminated with Cobalt. Minerals, 10.10.3390/min10010053 ↗
59. Bandura, L., Woszuk, A., Kołodyńska, D., and Franus, W. (2017). Application of Mineral Sorbents for Removal of Petroleum Substances: A Review. Minerals, 7.10.3390/min7030037 ↗
60. Characteristics of Adsorbents Used to Remove Petroleum Contaminants from Soil and Wastewater
61. Synthesis of Zeolite-Surface-Modified Perlite and Their Heavy Metal Adsorption Capability10.1016/j.matpr.2019.05.247 ↗
62. Study of Oil Sorption by Expanded Perlite at 298.15 K10.1016/j.seppur.2006.05.004 ↗
63. Effects of calcinated dolomite on the amendment of acid soil and release kinetics of Ca-Mg
64. Elastic Properties of Green Expanded Perlite Particle Compacts10.1016/j.powtec.2017.01.045 ↗
65. Biodegradation of the Emerging Contaminant 3-Nitro-1,2,4-Triazol-5-One and Its Product 3-Amino-1,2,4-Triazol-5-One in Perlite/Soil Columns10.1016/j.chemosphere.2023.139121 ↗
66. Remediation of an Oil-Contaminated Soil by Two Native Plants Treated with Biochar and Mycorrhizae10.1016/j.jenvman.2019.109755 ↗
67. Microbes-Assisted Phytoremediation of Lead and Petroleum Hydrocarbons Contaminated Water by Water Hyacinth10.1080/15226514.2023.2245905 ↗
68. (2024, March 06). VERVA DO—Fuel Premium. Available online: https://www.orlen.pl/pl/dla-biznesu/produkty/paliwa/oleje-napedowe/verva-on.
69. (2024, March 06). Unleaded Petrol 95. Available online: https://www.orlen.pl/pl/dla-biznesu/produkty/paliwa/benzyna/benzyna-bezolowiowa-95.
70. The Potential Use of Natural Expanded Perlite as a Flame Retardant Additive for Acrylonitrile-Butadiene-Styrene Based Composites10.1002/vnl.22047 ↗
71. Global Maize Production, Consumption and Trade: Trends and R&D Implications10.1007/s12571-022-01288-7 ↗
72. (2023). OECD-FAO Agricultural Outlook 2023–2032, Organisation for Economic Co-operation and Development.
73. (2024, March 07). DS1897B. Available online: https://www.corteva.co.uk/products-and-solutions/pioneer/DS1897B.html.
74. Wyszkowska, J., Boros-Lajszner, E., and Kucharski, J. (2024). The Impact of Soil Contamination with Lead on the Biomass of Maize Intended for Energy Purposes, and the Biochemical and Physicochemical Properties of the Soil. Energies, 17.10.3390/en17051156 ↗
75. Schinner, F., Ohlinger, R., Kandler, E., and Margesin, R. (1996). Methods in Soil Biology, Springer.10.1007/978-3-642-60966-4 ↗
76. Some Variables Affecting the Measurement of “Catalase Activity” in Soil10.2136/sssaj1964.03615995002800020024x ↗
77. Alef, K., and Nannipieri, P. (1988). Methods in Applied Soil Microbiology and Biochemistry, Academic London.
78. (2023, July 10). Available online: http://Statistica.Io.
79. Zaborowska, M., Wyszkowska, J., Borowik, A., and Kucharski, J. (2021). Bisphenol A—A Dangerous Pollutant Distorting the Biological Properties of Soil. Int. J. Mol. Sci., 22.10.3390/ijms222312753 ↗
80. Wyszkowska, J., Kucharski, J., Kucharski, M., and Borowik, A. (2013). Applicability of Biochemical Indices to Quality Assessment of Soil Polluted with Heavy Metal. J. Elem., 18.
81. (2023, October 18). Microsoft Office 365. Available online: www.office.com.
82. RStudio Team (2024, February 10). RStudio: Integrated Development for R. RStudio Version 2023.06.0, PBC, Boston, MA, USA. Available online: http://Www.Rstudio.Com/.
83. R Core Team R: A Language and Environment for Statistical Computing, R Foundation for Statistical Computing. Available online: https://www.R-Project.Org/.
84. Warnes, G.R., Bolker, B., Bonebakker, L., Gentleman, R., Huber, W., Liaw, A., Lumley, T., Maechler, M., Magnusson, M., and Moeller, S. (2024, February 10). Gplots: Various R Programming Tools for Plotting Data. Gplots 3.1.3. Available online: https://rdrr.io/cran/gplots/.
85. Circos: An Information Aesthetic for Comparative Genomics10.1101/gr.092759.109 ↗
86. Ma, D., Xu, J., Zhou, J., Ren, L., Li, J., Zhang, Z., Xia, J., Xie, H., and Wu, T. (2023). Using Sweet Sorghum Varieties for the Phytoremediation of Petroleum-Contaminated Salinized Soil: A Preliminary Study Based on Pot Experiments. Toxics, 11.10.3390/toxics11030208 ↗
87. Response of Avena Sativa, Microorganisms and Enzymes to Contamination of Soil with Diesel Oil10.17221/463/2015-PSE ↗
88. The Resistance of Lolium perenne L. × hybridum, Poa pratensis, Festuca rubra, F. arundinacea, Phleum pratense and Dactylis glomerata to Soil Pollution by Diesel Oil and Petroleum10.17221/42/2019-PSE ↗
89. Coupling Biostimulation and Phytoremediation for the Restoration of Petroleum Hydrocarbon-Contaminated Soil10.1080/15226514.2022.2103511 ↗
90. A Full-Scale Bioremediation Study of Diesel Fuel-Contaminated Soil: The Effect of Plant Species and Soil Amendments10.1007/s13762-023-05304-x ↗
91. Phytoremediation of Petroleum Hydrocarbon-Contaminated Saline-Alkali Soil by Wild Ornamental Iridaceae Species10.1080/15226514.2016.1225282 ↗
92. Application of Festuca Arundinacea in Phytoremediation of Soils Contaminated with Pb, Ni, Cd and Petroleum Hydrocarbons10.1016/j.ecoenv.2020.110409 ↗
93. Role of Festuca rubra and Festuca arundinacea in Determinig the Functional and Genetic Diversity of Microorganisms and of the Enzymatic Activity in the Soil Polluted with Diesel Oil10.1007/s11356-019-05888-3 ↗
94. Rule of Rhizobia and Bacillus in Phytoremediation of Contaminated Soil with Diesel Oil
95. Boros-Lajszner, E., Wyszkowska, J., Borowik, A., and Kucharski, J. (2021). Energetic Value of Elymus elongatus L. and Zea Mays L. Grown on Soil Polluted with Ni2+, Co2+, Cd2+, and Sensitivity of Rhizospheric Bacteria to Heavy Metals. Energies, 14.10.3390/en14164903 ↗
96. Phytotoxicity of Petroleum Hydrocarbons: Sources, Impacts and Remediation Strategies10.1016/j.envres.2021.111031 ↗
97. Manakov, A., and Edigarian, A. Analysis of Soil Contamination with Oil and Petroleum Products. Proceedings of the International Scientific Siberian Transport Forum TransSiberia—2021.
98. Stepanova, A.Y., Gladkov, E.A., Osipova, E.S., Gladkova, O.V., and Tereshonok, D.V. (2022). Bioremediation of Soil from Petroleum Contamination. Processes, 10.10.3390/pr10061224 ↗
99. Microorganisms in the Elimination of Oil Pollution Consequences (Review)10.1134/S0003683819040094 ↗
100. Investigation of Physical and Chemical Characteristics on Soil Due to Crude Oil Contamination and Its Remediation10.1007/s13201-019-0970-4 ↗