journal article·2022-07-18Open access

The Susceptibility to Biodegradation of Some Consolidants Used in the Restoration of Mural Paintings

Ioana Gomoiu, Roxana Cojoc, Robert Ruginescu, Simona Neagu, Mădălin Enache, Maria Dumbrăvician, Ioana Olteanu, Roxana Rădvan, Luminița Ghervase

Applied Sciences· Volume 12· Issue 14

Abstract

This study evaluated both the possible fungal metabolites involved in the degradation of the commercial consolidant known as Paraloid® B72 and the national artisanal consolidant named transparent dispersion of casein and the deteriorative potential of melanised fungi. Fungi were found to have the capacity to produce organic acids, proteases and esterases when they grow on consolidants, which can be used as nutrients. Mycelia produced by melanised fungi affected the appearance, as well as the integrity, of consolidants applied on painted experimental models and fragments of frescoes. In treatment trials with biocides (Biotin R®, Biotin T® and Preventol® RI 80), the morphology of the consolidants, as well as the vitality of the fungi, were assessed 30 days after the inoculation with melanic fungi. Direct observation, optical microscopy, colourimetry and microbiological analysis highlighted the degradation of the consolidants by fungi and their acquired resistance after biocidal treatments. Biotin R® applied by brushing on the surface of the consolidants proved to be the most effective treatment, followed by Biotin T®. Considering the overall results for both Paraloid® B72 and transparent dispersion of casein, use of Biotin R® applied by brushing is recommended for preventive conservation.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Building materials and conservation · Conservation Techniques and Studies · Cultural Heritage Materials Analysis

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Microbiology, Institute of Biology Bucharest of the Romanian Academy, 296, Splaiul Independentei, P.O. Box 56-53, 060031 Bucharest, Romania

Department of Conservation and Restoration, Faculty of Art History, Bucharest National University of Arts, 19, General Constantin Budișteanu, 010773 Bucharest, Romania

Centre of Excellence for Restoration by Optoelectronic Techniques, National Institute of Research and Development for Optoelectronics INOE 2000, 077125 Magurele, Romania

Funding

Executive Agency for Higher Education, Research, Development and Innovation Funding (UEFISCDI) · Romanian Academy

References (79)

Show source-provided references
1. Joseph, E. (2021). Microbial Growth and Its Effects on Inorganic Heritage Materials. Microorganisms in the Deterioration and Preservation of Cultural Heritage, Springer International Publishing.
2. Dark-Pigmented Biodeteriogenic Fungi in Etruscan Hypogeal Tombs: New Data on Their Culture-Dependent Diversity, Favouring Conditions, and Resistance to Biocidal Treatments10.1016/j.funbio.2021.03.003 ↗
3. Fungal Peritonitis Associated with Curvularia Geniculata and Pithomyces Species in a Patient with Vulvar Cancer Who Was Successfully Treated with Oral Voriconazole10.1038/ja.2013.108 ↗
4. Molecular Characterization of Airborne Fungi in Caves of the Mogao Grottoes, Dunhuang, China10.1016/j.ibiod.2011.04.006 ↗
5. Diversity and Seasonal Dynamics of Airborne Bacteria in the Mogao Grottoes, Dunhuang, China10.1007/s10453-011-9208-0 ↗
6. Zucconi, L., Canini, F., Isola, D., and Caneva, G. (2022). Fungi Affecting Wall Paintings of Historical Value: A Worldwide Meta-Analysis of Their Detected Diversity. Appl. Sci., 12.10.3390/app12062988 ↗
7. Dyda, M., Laudy, A., Decewicz, P., Romaniuk, K., Ciezkowska, M., Szajewska, A., Solecka, D., Dziewit, L., Drewniak, L., and Skłodowska, A. (2021). Diversity of Biodeteriorative Bacterial and Fungal Consortia in Winter and Summer on Historical Sandstone of the Northern Pergola, Museum of King John III’s Palace at Wilanow, Poland. Appl. Sci., 11.10.3390/app11020620 ↗
8. Pinna, D. (2021). Coping with Biological Grown on Stone Heritage Objects: Methods, Products, Applications, and Perspectives, Apple Academic Press.
9. Giannini, C., and Tapete, D. (2009). Materiali e Procedimenti Esecutivi Della Pittura Murale, Il Prato.
10. Fungal-Induced Deterioration of Mural Paintings: In Situ and Mock-Model Microscopy Analyses10.1017/S1431927616000544 ↗
11. Microbial Degradation of Paintings10.1128/AEM.65.3.879-885.1999 ↗
12. Biodecay of Cultural Heritage as a Space/Time-Related Ecological Situation—An Evaluation of a Series of Studies10.1016/S0964-8305(00)00107-4 ↗
13. Biodegradacion de Obras de Arte. Hongos Implicados En La Degradacion de Los Frescos Del Monasterio de La Rabida (Huelva)
14. Fungal Diversity and Its Contribution to the Biodeterioration of Mural Paintings in Two 1700-Year-Old Tombs of China10.1016/j.ibiod.2020.104972 ↗
15. Microbial Ability to Colonize Mural Painting and Its Substrate10.12693/APhysPolA.134.383 ↗
16. Ruginescu, R., Enache, M., Popescu, O., Gomoiu, I., Cojoc, R., Batrinescu-Moteau, C., Maria, G., Dumbravician, M., and Neagu, S. (2022). Characterization of Some Salt-Tolerant Bacterial Hydrolases with Potential Utility in Cultural Heritage Bio-Cleaning. Microorganisms, 10.10.3390/microorganisms10030644 ↗
17. Material Characterization and Biodegradation Assessment of Mural Paintings: Renaissance Frescoes from Santo Aleixo Church, Southern Portugal10.1080/15583058.2012.751466 ↗
18. Study of the Euendolithic Activity of Blackmeristematic Fungi Isolated from a Marble Statue in the Quirinale Palace’s Gardens InRome, Italy10.1007/s10347-019-0564-5 ↗
19. Biological Colonization on Stone Monuments: A New Low Impact Cleaning Method10.1016/j.culher.2017.09.004 ↗
20. Description of Aeminiaceae Fam. Nov., Aeminium Gen. Nov. and Aeminium ludgeri sp. Nov. (Capnodiales), Isolated from a Biodeteriorated Art-Piece in the Old Cathedral of Coimbra, Portugal10.3897/mycokeys.45.31799 ↗
21. In Vitro Biodegradation Potential of Airborne Aspergilli and Penicillia10.1007/s00114-019-1603-3 ↗
22. Changes in Heterotrophic Microbial Communities Induced by Biocidal Treatments in the Monastery of San Martiño Pinario (Santiago de Compostela, NW Spain)10.1016/j.ibiod.2020.105130 ↗
23. Melding the Old with the New: Trends in Methods Used to Identify, Monitor, and Control Microorganisms on Cultural Heritage Materials10.1007/s00248-016-0770-4 ↗
24. Tertiary Bioreceptivity of Schists from Prehistoric Rock Art Sites in the Côa Valley (Portugal) and Siega Verde (Spain) Archaeological Parks: Effects of Cleaning Treatments10.1016/j.ibiod.2019.05.011 ↗
25. Medium-Term Field Evaluation of Several Widely Used Cleaning-Restoration Techniques Applied to Algal Biofilm Formed on a Granite-Built Historical Monument10.1016/j.ibiod.2019.104870 ↗
26. Learning from the Past, Intervening in the Present: The Role of Conservation Science in the Challenging Restoration of the Wall Painting Marriage at Cana by Luca Longhi (Ravenna, Italy)10.1186/s40494-020-0354-y ↗
27. Controlling Biodeterioration of Cultural Heritage Objects with Biocides: A Review10.1016/j.ibiod.2019.104721 ↗
28. White Fungal Covering on the Wall Paintings of the Saint-Savin-Sur-Gartempe Abbey Church Crypt: A Case Study10.1016/j.ibiod.2017.04.007 ↗
29. Di Turo, F., and Medeghini, L. (2021). How Green Possibilities Can Help in a Future Sustainable Conservation of Cultural Heritage in Europe. Sustainability, 13.10.3390/su13073609 ↗
30. Raman and ATR FT-IR Investigations of Innovative Silica Nanocontainers Loaded with a Biocide for Stone Conservation Treatments10.1016/j.microc.2020.104766 ↗
31. Formulation of Mesoporous Silica Nanoparticles for Controlled Release of Antimicrobials for Stone Preventive Conservation10.3389/fchem.2020.00699 ↗
32. Green Biocides to Control Biodeterioration in Materials Science and the Example of Preserving World Heritage Monuments10.1016/j.cogsc.2020.100359 ↗
33. Green Extraction of Natural Products. Origins, Current Status, and Future Challenges10.1016/j.trac.2019.05.037 ↗
34. Lo Schiavo, S., De Leo, F., and Urzì, C. (2020). Present and Future Perspectives for Biocides and Antifouling Products for Stone-Built Cultural Heritage: Ionic Liquids as a Challenging Alternative. Appl. Sci., 10.10.3390/app10186568 ↗
35. Negri, A., Nervo, M., Di Marcello, S., and Castelli, D. (2021). Consolidation and Adhesion of Pictorial Layers on a Stone Substrate. The Study Case of the Virgin with the Child from Palazzo Madama, in Turin. Coatings, 11.10.3390/coatings11060624 ↗
36. Artesani, A., Di Turo, F., Zucchelli, M., and Traviglia, A. (2020). Recent Advances in Protective Coatings for Cultural Heritage–An Overview. Coatings, 10.10.3390/coatings10030217 ↗
37. Nanomaterials in Art Conservation10.1038/nnano.2015.38 ↗
38. Literature Review: The Use of Paraloid B-72 as a Surface Consolidant for Stained Glass10.1179/019713603806112813 ↗
39. Comparative and Experimental Studies for Evaluation of Paraloid B-72 in Traditional and Nano Forms for Joining of Pottery Samples10.4028/www.scientific.net/JNanoR.61.61 ↗
40. Effect of Solvent on PARALOID® B72 and B44 Acrylic Resins Used as Adhesives in Conservation10.1186/s40494-019-0283-9 ↗
41. Modification of EAPC-XYL by Pseudomonas Lipases Bacteria to Remove Acrylic from the Mural Oil Paintings10.21608/shedet.006.10 ↗
42. Synthetic Consolidants Attacked by Melanin-Producing Fungi: Case Study of the Biodeterioration of Milan (Italy) Cathedral Marble Treated with Acrylics10.1128/AEM.02220-06 ↗
43. Istudor, I., and Ciobanu, G. (1982). Dispersii de Cazeinat de Calciu Folosite În Conservarea Picturilor Murale Iîn Frescă Și Tempera., Muzeul Naţional de Istorie.
44. Assessment of Indoor Air Environment of a Nigerian Museum Library and Its Biodeteriorated Books Using Culture-Dependent and –Independent Techniques10.1016/j.ibiod.2018.03.003 ↗
45. Extremotolerant Rock Inhabiting Black Fungi from Italian Monumental Sites10.1007/s13225-015-0342-9 ↗
46. Gomoiu, I., Chatzitheodoridis, E., Vadrucci, S., and Walther, I. (2013). The Effect of Spaceflight on Growth of Ulocladium chartarum Colonies on the International Space Station. PLoS ONE, 8.10.1371/journal.pone.0062130 ↗
47. Ruginescu, R., Gomoiu, I., Popescu, O., Cojoc, R., Neagu, S., Lucaci, I., Batrinescu-Moteau, C., and Enache, M. (2020). Bioprospecting for Novel Halophilic and Halotolerant Sources of Hydrolytic Enzymes in Brackish, Saline and Hypersaline Lakes of Romania. Microorganisms, 8.10.3390/microorganisms8121903 ↗
48. The Quality of Air at Archives and the Biodeterioration of Photographs10.1016/j.ibiod.2009.12.005 ↗
49. Unković, N., Dimkić, I., Stupar, M., Stanković, S., Vukojević, J., and Ljaljević Grbić, M. (2018). Biodegradative Potential of Fungal Isolates from Sacral Ambient: In Vitro Study as Risk Assessment Implication for the Conservation of Wall Paintings. PLoS ONE, 13.10.1371/journal.pone.0190922 ↗
50. Diversity and Bioprospecting of Extremely Halophilic Archaea Isolated from Algerian Arid and Semi-Arid Wetland Ecosystems for Halophilic-Active Hydrolytic Enzymes10.1016/j.micres.2017.12.011 ↗
51. Bio-Removal of Black Crust from Marble Surface: Comparison with Traditional Methodologies and Application on a Sculpture from the Florence’s English Cemetery10.1016/j.proche.2013.03.017 ↗
52. Removal of Acrylic Coatings from Works of Art by Means of Nanofluids: Understanding the Mechanism at the Nanoscale10.1039/c0nr00255k ↗
53. Evaluation of Polyvinyl Alcohol–Borax/Agarose (PVA–B/AG) Blend Hydrogels for Removal of Deteriorated Consolidants from Ancient Egyptian Wall Paintings10.1186/s40494-019-0264-z ↗
54. (1977). CIE Recommendations on Uniform Color Spaces, Color-Difference Equations, and Metric Color Terms. Color Res. Appl., 2, 5–6.10.1002/j.1520-6378.1977.tb00102.x ↗
55. Production of Gluconic Acid from Glucose by Aspergillus niger: Growth and Non-Growth Conditions10.1016/S0032-9592(03)00270-X ↗
56. A Study of Organic Acid Production in Contrasts between Two Phosphate Solubilizing Fungi: Penicillium oxalicum and Aspergillus niger10.1038/srep25313 ↗
57. Humidity Governs the Wall-Inhabiting Fungal Community Composition in a 1600-Year Tomb of Emperor Yang10.1038/s41598-020-65478-z ↗
58. Biodeteriogenic and Toxigenic Agents on 17th Century Mural Paintings and Façade of the Old Church of the Holy Ascension (Veliki Krčimir, Serbia)10.1177/1420326X15587178 ↗
59. Mycological and Ultrastructural Studies to Evaluate Biodeterioration of Mural Paintings. Detection of Fungi and Mites in Frescos of the Monastery of St Damian in Assisi10.1016/0964-8305(94)90066-3 ↗
60. A Comparative Study of the Fungal Flora Present in Medieval Wall Paintings in the Chapel of the Castle Herberstein and in the Parish Church of St Georgen in Styria, Austria10.1016/S0964-8305(97)00062-0 ↗
61. Microbiological Survey for Analysis of the Brown Spots on the Walls of the Tomb of King Tutankhamun10.1016/j.ibiod.2013.01.014 ↗
62. Caneva, G., Isola, D., Lee, H.J., and Chung, Y.J. (2020). Biological Risk for Hypogea: Shared Data from Etruscan Tombs in Italy and Ancient Tombs of the Baekje Dynasty in Republic of Korea. Appl. Sci., 10.10.3390/app10176104 ↗
63. Invasion of the French Paleolithic Painted Cave of Lascaux by Members of the Fusarium solani Species Complex10.1080/15572536.2007.11832546 ↗
64. Entomogenous Fungi and the Conservation of the Cultural Heritage: A Review10.1016/j.ibiod.2008.05.002 ↗
65. Contributions to the Study of the Biodeterioration of the Work of Art and of Historic Monuments. II. Species of Fungi Involved in the Deterioration of Mural Paintings from the Monasteries of Moldavia
66. Micromycetes Taking Part in Deterioration of Old Russian Wall Paintings
67. Heterotrophic Microorganisms in Air and Biofilm Samples from Roman Catacombs, with Special Emphasis on Actinobacteria and Fungi10.1016/j.ibiod.2003.12.003 ↗
68. The Community Distribution of Bacteria and Fungi on Ancient Wall Paintings of the Mogao Grottoes10.1038/srep07752 ↗
69. Analysis and Comparison of the Microflora Isolated from Fresco Surface and from Surrounding Air Environment through Molecular and Biodegradative Assays10.1007/s11274-012-1004-7 ↗
70. Contamination of Mural Paintings by Indoor Airborne Fungal Spores10.1007/BF02066254 ↗
71. Deterioration of Medieval Painting in the Chapel of the Holy Nail, Siena (Italy) Partially Treated with Paraloid B7210.1016/j.ibiod.2009.03.004 ↗
72. In Vitro Analyses of Fungi and Dolomitic Limestone Interactions: Bioreceptivity and Biodeterioration Assessment10.1016/j.ibiod.2020.105107 ↗
73. Occurrence of Aspergillus allahabadii on Sandstone at Bayon Temple, Angkor Thom, Cambodia10.1016/j.ibiod.2012.06.022 ↗
74. Biodeterioration of Acrylic Polymers Paraloid B-72 and B-44: Report on Field Trials
75. Extracellular Enzymatic Profile of Fungal Deteriogens of Historical Palace of Ujjain
76. Fungal Biodeterioration in Historic Buildings of Havana (Cuba)10.1080/00173134.2011.643920 ↗
77. A Preliminary Note on the Use of Enzymes in Conservation: The Removal of Aged Acrylic Resin Coatings with Lipase10.1179/sic.1999.44.4.278 ↗
78. Investigation of Fungal Deterioration of Synthetic Paint Binders Using Vibrational Spectroscopic Techniques10.1002/mabi.200400134 ↗
79. Differences of Microbial Community on the Wall Paintings Preserved in Situ and Ex Situ of the Tiantishan Grottoes, China10.1016/j.ibiod.2018.02.013 ↗