Cold Storage Media versus Optisol-GS in the Preservation of Corneal Quality for Keratoplasty: A Systematic Review
Source abstract
Abstract
Optisol-GS is the most widely used pharmaceutical composition to preserve corneas for transplantation. This systematic review investigated the effects of different cold corneal storage media (CCSM) compared with Optisol-GS on the quality of stored corneas. The literature was searched throughout May 2022 on six databases and grey literature. Studies including corneas (population) exposed to distinct cold storage media (exposure) and Optisol-GS (comparison) that reported qualitative and/or quantitative parameters of cornea quality (outcome) were included. Methodological quality was assessed using ToxRTool. From 4520 identified studies, fourteen were included according to the eligibility criteria, comprising 769 evaluated cornea samples comparing Optisol-GS with commercial and noncommercial media. All studies showed good methodological quality. Experimental times ranged from 1–28 days, mainly using 4 °C as the preservation temperature. Viable endothelial cell density (ECD) and endothelial cell morphology (EC) were the most assessed parameters. ECD results for Cornisol were higher than Optisol-GS in 10 days (p = 0.049) and favored Cornea ColdTM up to 4 weeks (p < 0.05), which also showed better qualitative results. While the standardization of test protocols could improve comparisons, evidence indicates that most CCSM present similar performances on cornea preservation for transplantation at seven days, while some formulations may increase preservation at extended times.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Corneal surgery and disorders · Corneal Surgery and Treatments · Glaucoma and retinal disorders
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Post-Graduation Program in Science and Biotechnology, Fluminense Federal University, Niterói 24020-140, Brazil
National Institute for Quality Control in Health, Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, Brazil
Department of Pediatric Dentistry and Orthodontics, Faculty of Dentistry, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-971, Brazil
Department of General Biology, Fluminense Federal University, Niterói 24020-140, Brazil
Brazilian Centre for Validation of Alternative Methods (BraCVAM), Oswaldo Cruz Foundation, Rio de Janeiro 21040-900, Brazil
Cell and Molecular Biology Department, Institute of Biology, Fluminense Federal University, Niteroi 24020-140, Brazil
Clinical Research Unit, Antônio Pedro Hospital, Fluminense Federal University, Niteroi 24033-900, Brazil
Funding
INOVA FIOCRUZ, Rio de Janeiro, Brazil
References (44)
Show source-provided references
1. Ten years of corneal donation to the Hospital São Paulo Eye Bank: Characteristics of cornea donors from 1996 to 2005
2. Corneal transplantation in the modern era10.4103/ijmr.IJMR_141_19 ↗
3. Global survey of corneal transplantation and eye banking10.1001/jamaophthalmol.2015.4776 ↗
4. A brief review of corneal transplantation: Techniques, indications and methods of corneal preservation
5. Optisol corneal storage medium., Optisol corneal storage medium10.1016/S0002-9394(14)71803-3 ↗
6. Mannis, M.J., and Holland, E.J. (2021). Cornea, E-Book, Elsevier Health Sciences. [5th ed.].
7. Procurement, storage and utilization trends of eye banks in India10.4103/ijo.IJO_1551_18 ↗
8. Efficacy and safety of gentamicin and streptomycin in Optisol-GS, a preservation medium for donor corneas10.1097/00003226-199501000-00009 ↗
9. Optisol containing streptomycin10.1016/S0002-9394(14)71413-8 ↗
10. Combination antibiotic supplementation of corneal storage medium10.1016/S0002-9394(14)73579-2 ↗
11. Preserving the cornea: Corneal storage media10.1097/01.icu.0000233950.63853.88 ↗
12. Comparison of structural integrity and functional status of corneal endothelium stored in Cornisol and Optisol-GS
13. A quantitative method to evaluate the donor corneal tissue quality used in a comparative study between two hypothermic preservation media10.1007/s10561-014-9424-2 ↗
14. The PRISMA 2020 statement: An updated guideline for reporting systematic reviews
15. “ToxRTool”, a new tool to assess the reliability of toxicological data10.1016/j.toxlet.2009.05.013 ↗
16. Changes in corneal endothelial apical junctional protein organization after corneal cold storage10.1097/00003226-199911000-00015 ↗
17. Effect of a newly developed corneal storage medium on corneal endothelium--morphological study by scanning electron microscopy
18. Significative lengthening of the time of cornea conservation supporting its natural tension and curvature10.31053/1853.0605.v59.n2.38487 ↗
19. In-vitro comparative study of a locally prepared corneal storage medium and Optisol
20. Development of novel corneal storage medium: First report. Examinations of rabbit cornea10.1016/S0021-5155(02)00502-6 ↗
21. Evaluation of various preservation media for storage of donor corneas10.4103/ijo.IJO_258_21 ↗
22. A Prospective, In Vitro, Randomized Study to Compare Two Media for Donor Corneal Storage10.1097/ICO.0000000000000912 ↗
23. Human cornea conservation in coconut water solution10.5935/0004-2749.20210027 ↗
24. The effect of light exposure on the efficacy and safety of amphotericin B in corneal storage media10.1001/jamaophthalmol.2016.0008 ↗
25. Optisol vs
26. Sinasol versus Optisol-GS for cold preservation of human cornea: A prospective ex vivo and clinical study10.1007/s10561-021-09930-y ↗
27. Comparing quantitative and qualitative indices of the donated corneas maintained in Optisol-GS with those kept in Eusol-C10.1007/s10561-014-9466-5 ↗
28. Efficacy and safety of antifungal additives in Optisol-GS corneal storage medium10.1001/jamaophthalmol.2014.397 ↗
29. The safety and efficacy of linezolid and daptomycin as an additive in optisol-GS against methicillin-resistant Staphylococcus aureus10.1097/ICO.0b013e318226c6b3 ↗
30. A new storage medium containing amphotericin B versus Optisol-GS for preservation of human donor corneas
31. In vitro comparison of Chen medium and Optisol-GS medium for human corneal storage10.1097/00003226-200011000-00005 ↗
32. Performance of new hypothermic corneal storage media with an antimycotic tablet in comparison to traditional hypothermic media during simulated eye bank processing10.1097/ICO.0000000000002333 ↗
33. [Development of corneal storage medium—Second report. Examination of human cornea]
34. Comparison of Donor Cornea Storage in Optisol-GS and the Central Eye Bank of Iran (CEBI) Medium
35. EBAA Medical Standards
36. Dubashynskaya, N., Poshina, D., Raik, S., Urtti, A., and Skorik, Y.A. (2019). Polysaccharides in ocular drug delivery. Pharmaceutics, 12.10.3390/pharmaceutics12010022 ↗
37. European Eye Bank Association (EEBA) (2020). Technical Guidelines for Ocular Tissue, European Eye Bank Association (EEBA).
38. Corneal temperature reversal after storage in Chen medium compared with Optisol GS10.1097/00003226-200107000-00012 ↗
39. A randomized, double-masked clinical trial of Optisol-GS vs
40. Comparison of Chen Medium and Optisol-GS for human corneal preservation at 4 °C: Results of transplantation10.1097/00003226-200110000-00003 ↗
41. Efficacy and safety of voriconazole as an additive in Optisol GS: A preservation medium for corneal donor tissue10.1097/ICO.0b013e31802d82e8 ↗
42. Improvement of human corneal endothelium in culture after prolonged hypothermic storage10.1177/1120672103013009-1002 ↗
43. Glutamine and fatty acid oxidation are the main sources of energy for Kupffer and endothelial cells
44. Erickson, K.E. (2022, June 28). MLIS, American Academy of Ophtalmology. Imported EusolC to Fill the Gap Created by Optisol Shortage. Available online: https://www.aao.org/headline/imported-eusolc-to-fill-gap-created-by-optisol-sho.