Chemical Composition and Antimicrobial Activity of Essential Oils and Hydrosols from Oregano, Sage and Pennyroyal against Oral Pathogens
Source abstract
Abstract
In this study, the chemical composition of essential oils (EOs) extracted from Origanum vulgare ssp. hirtum Lamiaceae, (oregano), Salvia officinalis Lamiaceae (sage), Mentha pulegium Lamiaceae (pennyroyal), and respective hydrosols (HSs) has been investigated by Gas Chromatography–Mass Spectrometry (GC-MS). The antimicrobial activity was assessed against two oral pathogens: Gram-positive bacterium Streptococcus mutans and the fungus Candida albicans by determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal/Fungicidal concentration (MBC/MFC). Three-fold diluted solutions were dispensed into each well of a 96-well microtiter plate and, after incubation, MIC was determined by visual monitoring. The MBC/MFC was determined by transferring a small quantity of sample contained in each replicate well of the microtiter plates to appropriate culture media using a microplate replicator. The EOs of the tested herbs showed antimicrobial properties, especially the EO oil of O. vulgare, which exerted the highest antimicrobial activity. HSs of S. officinalis and M. pulegium exerted no antimicrobial activity, in contrast to oregano HS, which displayed strong antimicrobial activity. In all cases, a higher number of compounds were detected in EOs than in the corresponding HSs. The major compounds of sage EO were detected to be α-thujone (25.1%), 1,8-cineole (15.8%) and β-pinene (10.0%), while the HS was characterized by the presence of 1,8-cineole (32.6%), borneol (22.6%) and α-thujone (22.4%). Pennyroyal EO and HS consists mainly of pulegone (62.1 and 50.6%, respectively). Carvacrol was the major component present in EO (63%) and HS (97.3%) of oregano, probably contributing to the antimicrobial activity. Further research is needed in order to elucidate the antimicrobial mechanisms of specific compounds present in essential oils and hydrosols of Lamiaceae grown in Greece against oral pathogens.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Essential Oils and Antimicrobial Activity · Phytochemistry and Biological Activities · Plant chemical constituents analysis
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Laboratory of Microbial Biotechnology, Molecular Bacteriology-Virology, Department of Biochemistry & Biotechnology, School of Health Sciences, University of Thessaly, 41500 Larissa, Greece
Laboratory of Chemistry, Department of Food Science and Human Nutrition, School of Food and Nutrition Sciences, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece
References (79)
Show source-provided references
1. Diabetes mellitus and oral health10.1007/s12020-014-0496-3 ↗
2. Streptococcus mutans, caries and simulation models10.3390/nu2030290 ↗
3. The Biology of Streptococcus mutans10.1128/microbiolspec.GPP3-0051-2018 ↗
4. Evaluation of anti-enzyme properties of Origanum vulgare essential oil against oral Candida albicans10.1016/j.mycmed.2017.12.001 ↗
5. Oral candidiasis10.1016/j.clindermatol.2016.02.022 ↗
6. Role of glucosyltransferase B in interactions of Candida albicans with Streptococcus mutans and with an experimental pellicle on hydroxyapatite surfaces10.1128/AEM.05203-11 ↗
7. Essential Oils, A New Horizon in Combating Bacterial Antibiotic Resistance10.2174/1874285801408010006 ↗
8. Interaction of carvacroland voriconazole against drug-resistant Candida strains isolated from patients with candidiasis10.1016/j.mycmed.2018.11.001 ↗
9. Cieplik, F., Jakubovics, N.S., Buchalla, W., Maisch, T., Hellwig, E., and Al-Ahmad, A. (2019). Resistance toward chlorhexidine in oral bacteria-is there cause for concern?. Front. Microbiol., 10.10.3389/fmicb.2019.00587 ↗
10. The significance of essential oils and their antifungal properties in the food industry: A systematic review10.1016/j.heliyon.2023.e21386 ↗
11. Hou, T., Sana, S.S., Li, H., Xing, Y., Nanda, A., Netala, V.R., and Zhang, Z. (2022). Essential oils and its antibacterial, antifungal and anti-oxidant activity applications: A review. Food Biosci., 47.10.1016/j.fbio.2022.101716 ↗
12. Essential oils from aromatic herbs as antimicrobial agents10.1016/j.copbio.2011.08.005 ↗
13. Hydrosols: Biological activity and potential as antimicrobials for food applications10.1016/j.foodcont.2017.10.030 ↗
14. García-Salinas, S., Elizondo-Castillo, H., Arruebo, M., Mendoza, G., and Irusta, S. (2018). Evaluation of the antimicrobial activity and cytotoxicity of different components of natural origin present in essential oils. Molecules, 23.10.1101/325639 ↗
15. Chouhan, S., Sharma, K., and Guleria, S. (2017). Antimicrobial Activity of Some Essential Oils—Present Status and Future Perspectives. Medicines, 4.10.3390/medicines4030058 ↗
16. Antioxidant activity of essential oils10.1021/jf403496k ↗
17. Salvia officinalis in dentistry10.4103/2155-8213.150870 ↗
18. Hydrolates: By-products of essential oil distillation: Chemical composition, biological activity and potential uses10.5937/savteh2002054A ↗
19. Variability in essential oil content and composition of Origanum hirtum L., Origanum onites L., Coridothymus capitatus (L.) and Satureja thymbra L. populations from the Greek island Ikaria10.1016/j.indcrop.2010.10.021 ↗
20. Irrigation and nitrogen application affect Greek oregano (Origanum vulgare ssp. hirtum) dry biomass, essential oil yield and composition10.1016/j.indcrop.2020.112392 ↗
21. Algerian Mentha pulegium L. leaves essential oil: Chemical composition, antimicrobial, insecticidal and antioxidant activities10.1016/j.indcrop.2016.08.042 ↗
22. Chemical composition and anticancer activity of essential oils of Mediterranean sage (Salvia officinalis L.) grown in different environmental conditions10.1016/j.fct.2012.12.036 ↗
23. Harvesting time influences the yield and oil composition of Origanum vulgare L. ssp. vulgare and ssp. hirtum10.1016/j.indcrop.2013.04.024 ↗
24. Exploring the nexus between the composition of essential oil and the bacterial phytobiome associated with different compartments of the medicinal plants Origanum vulgare ssp. vulgare, O. vulgare ssp. hirtum, and O. heracleoticum10.1016/j.indcrop.2022.115997 ↗
25. Chemical composition of Mentha pulegium and Rosmarinus officinalis essential oils and their antileishmanial, antibacterial and antioxidant activities10.1016/j.micpath.2017.08.015 ↗
26. Chemical composition, antioxidant and antimicrobial properties of Mentha pulegium, Lavandula stoechas and Satureja calamintha Scheele essential oils and an evaluation of their bactericidal effect in combined processes10.1016/j.ifset.2013.12.016 ↗
27. Antibacterial activity of the essential oils of Salvia officinalis L. and Salvia triloba L. cultivated in South Brazil10.1016/j.foodchem.2005.09.078 ↗
28. The effect of various extraction techniques on the quality of sage (Salvia officinalis L.) essential oil, expressed by chemical composition, thermal properties and biological activity
29. Tunisian Salvia officinalis L. and Schinus molle L. essential oils: Their chemical compositions and their preservative effects against Salmonella inoculated in minced beef meat10.1016/j.ijfoodmicro.2008.04.005 ↗
30. Antibacterial activity of Turkish spice hydrosols
31. An optimal control approach to steam distillation of essential oils from aromatic plants10.1016/j.compchemeng.2018.05.009 ↗
32. Cleaner production of essential oils by steam distillation10.1016/j.jclepro.2004.02.039 ↗
33. Distillation time changes oregano essential oil yields and composition but not the antioxidant or antimicrobial activities10.21273/HORTSCI.47.6.777 ↗
34. Salvia officinalis L. essential oils: Effect of hydrodistillation time on the chemical composition, antioxidant and antimicrobial activities10.1080/14786419.2010.499513 ↗
35. Essential oil yield and composition of garden sage as a function of different steam distillation times10.21273/HORTSCI.49.6.785 ↗
36. Effect of distillation time on mentha canadensis essential oil yield and composition10.21273/HORTSCI.47.5.643 ↗
37. Comparative chemotype determination of Lamiaceae plants by means of GC–MS, FT-IR, and dispersive-Raman spectroscopic techniques and GC-FID quantification10.1016/j.indcrop.2014.08.003 ↗
38. A Retention Index Calculator Simplifi es Identifi cation of Plant Volatile Organic Compounds10.1002/pca.1137 ↗
39. Antibacterial activity of honeys produced in Mount Olympus area against nosocomial and foodborne pathogens is mainly attributed to hydrogen peroxide and proteinaceous compounds10.1080/00218839.2019.1649570 ↗
40. Didaras, N.A., Kafantaris, I., Dimitriou, T.G., Mitsagga, C., Karatasou, K., Giavasis, I., Stagos, D., Amoutzias, G.D., Hatjina, F., and Mossialos, D. (2021). Biological properties of bee bread collected from apiaries located across Greece. Antibiotics, 10.10.3390/antibiotics10050555 ↗
41. Pfaller, M.A., Haturvedi, V., Espinel-Ingroff, A., Ghannoum, M.A., Gosey, L.L., Odds, F.C., Rex, J.H., Rinaldi, M.G., Sheehan, D.J., and Walsh, T.J. (2002). Approved Standard—Second Edition Serving the World’ s Medical Science Community Through Voluntary Consensus, NCCLS.
42. Field studies of nitrogen application on Greek oregano (Origanum vulgare ssp. hirtum (Link) Ietswaart) essential oil during two cultivation seasons10.1016/j.indcrop.2013.01.021 ↗
43. A kinetic study of essential oil components distillation for the recovery of carvacrol rich fractions
44. Date of harvesting affects yields and quality of Origanum vulgare ssp. hirtum (Link) Ietswaart10.1002/jsfa.9805 ↗
45. Węglarz, Z., Kosakowska, O., Przybył, J.L., Pióro-Jabrucka, E., and Baczek, K. (2020). The Quality of Greek Oregano (O. vulgare L. subsp. hirtum (Link) Ietswaart) and Common Oregano (O. vulgare L. subsp. vulgare) Cultivated in the Temperate Climate of Central Europe. Foods, 9.10.3390/foods9111671 ↗
46. The effect of γ-irradiation on chemical composition, microbial load and sensory properties of Sicilian oregano10.1016/j.lwt.2016.05.025 ↗
47. Chemical profile and in vitro antibacterial potential of essential oils and hydrolat extracts from aerial parts of three wild species of Moroccan Thymus
48. Cultivation techniques and drying process can affect the inflorescence essential oil composition of three selections of Salvia officinalis10.1016/j.indcrop.2022.114923 ↗
49. Comparison of essential oil composition and phenolic acid content of selected Salvia species measured by GC-MS and HPLC methods10.1016/j.indcrop.2015.02.047 ↗
50. Harvesting season and plant part dependent variations in the essential oil composition of Salvia officinalis L. grown in northern India10.1016/j.hermed.2015.04.004 ↗
51. Antiviral activity of Lavandula angustifolia L. and Salvia officinalis L. essential oils against avian influenza H5N1 virus
52. Capillary gas chromatography-mass spectrometry of volatile and semi-volatile compounds of Salvia officinalis10.1016/j.chroma.2003.11.046 ↗
53. Salvia officinalis essential oil: Chemical analysis and evaluation of anti- enzymatic and antioxidant bioactivities10.1016/j.sajb.2018.07.010 ↗
54. European pennyroyal (Mentha pulegium) from Portugal: Chemical composition of essential oil and antioxidant and antimicrobial properties of extracts and essential oil10.1016/j.indcrop.2011.08.011 ↗
55. Chemical composition and antimicrobial properties of Moroccan Mentha pulegium L. essential oil10.1016/j.matpr.2022.09.318 ↗
56. Essential oil variation and antioxidant capacity of Mentha pulegium populations and their relation to ecological factors10.1016/j.bse.2020.104084 ↗
57. Carvacrol and human health: A comprehensive review10.1002/ptr.6103 ↗
58. Effects of water stress on lavender and sage biomass production, essential oil composition and biocidal properties against Tetranychus urticae (Koch)10.1016/j.scienta.2016.10.024 ↗
59. Essential oil yields and qualities of different clonal lines of Salvia lavandulifolia monitored in Spain over four years of cultivation10.1016/j.indcrop.2015.11.010 ↗
60. The composition of the essential oil and aqueous distillate of Origanum vulgare L. growing in Saudi Arabia and evaluation of their antibacterial activity10.1016/j.arabjc.2018.02.008 ↗
61. Microclimatic buffering on medicinal and aromatic plants: A review10.1016/j.indcrop.2020.113144 ↗
62. Effect of plant growth regulators and salt stress on secondary metabolite composition in Lamiaceae species10.1016/j.sajb.2021.10.030 ↗
63. De Activity Evaluation
64. Antibiotic side effects10.1016/S0025-7125(05)70309-6 ↗
65. Kyriakidis, I., Tragiannidis, A., München, S., and Andreas, H. (2017). Clinical Hepatotoxicity Associated with Antifungal Agents, Taylor and Francis.10.1080/14740338.2017.1270264 ↗
66. Pandey, A.K., Kumar, P., Singh, P., Tripathi, N.N., and Bajpai, V.K. (2017). Essential oils: Sources of antimicrobials and food preservatives. Front. Microbiol., 7.10.3389/fmicb.2016.02161 ↗
67. Selected essential oil vapours inhibit growth of Aspergillus spp. in oats with improved consumer acceptability10.1016/j.indcrop.2016.11.044 ↗
68. The antifungal activity of essential oils in combination with warm air flow against postharvest phytopathogenic fungi in apples10.1016/j.foodcont.2016.03.024 ↗
69. Cellulose acetate active films incorporated with oregano (Origanum vulgare) essential oil and organophilic montmorillonite clay control the growth of phytopathogenic fungi10.1016/j.fpsl.2016.07.001 ↗
70. Napoli, E., Giovino, A., Carrubba, A., Siong, V.H.Y., Rinoldo, C., Nina, O., and Ruberto, G. (2020). Variations of essential oil constituents in oregano (Origanum vulgare subsp. viridulum (= o. heracleoticum) over cultivation cycles. Plants, 9.10.3390/plants9091174 ↗
71. In vitro activity of essential oils extracted from plants used as spices against fluconazole-resistant and fluconazole-susceptible Candida spp.10.1139/W08-097 ↗
72. Combination of Carvacrol and Thymol: Antimicrobial Activity Against Staphylococcus aureus and Antioxidant Activity10.1089/fpd.2018.2594 ↗
73. Leyva-l, N., Guti, E.P., Vazquez-olivo, G., and Heredia, J.B. (2017). Essential Oils of Oregano: Biological Activity beyond Their Antimicrobial Properties. Molecules, 22.10.3390/molecules22060989 ↗
74. Essential oils from Origanum vulgare and Salvia officinalis exhibit antibacterial and anti-biofilm activities against Streptococcus pyogenes10.1016/j.micpath.2018.02.026 ↗
75. The antibacterial effect of sage extract (Salvia officinalis) mouthwash against Streptococcus mutans in dental plaque: A randomized clinical trial
76. Chemical Composition, Antioxidant Activities of the Essential Oil of Mentha pulegium L., South East of Algeria
77. European Medicines Agency (2016). Public Statement on the Use of Herbal Medicinal Products Containing Pulegone and Menthofuran, European Medicines Agency.
78. Politi, M., Menghini, L., Conti, B., Bedini, S., Farina, P., Cioni, P.L., Braca, A., and De Leo, M. (2020). Reconsidering Hydrosols as Main Products of Aromatic Plants Manufactory: The Lavandin (Lavandula × intermedia) Case Study in Tuscany. Molecules, 25.10.3390/molecules25092225 ↗
79. Sage tea–thyme–peppermint hydrosol oral rinse reduces chemotherapy-induced oral mucositis: A randomized controlled pilot study10.1016/j.ctim.2016.05.010 ↗