Use of Fourier-Transform Infrared Spectroscopy for DNA Identification on Recycled PET Composite Substrate
Source abstract
Abstract
Fourier-transform infrared (FTIR) spectroscopy has been extensively used in plastic pollution research, since it has the advantages of great simplicity, rapidity, and low cost, being widely employed in the fingerprint identification of molecular composition and structure. The present study evaluates attenuated total reflection (ATR)–FTIR spectroscopy as a sensitive and effective assay for the identification of deoxyribonucleic acid (DNA) isolated from experimental animals. Various composite materials based on recycled polyethylene terephthalate (PET) as the main component, along with high-density polyethylene (HDPE), polypropylene (PP), and aluminum nanopowder obtained using an injection-molding machine, were used as substrate contaminants. The contamination was performed using quantified nucleic acid solution added in droplets to the clean, decontaminated samples, which were then dried and kept in a protective environment until the analysis. ATR–FTIR (with an FTIR spectrometer equipped with an ATR accessory) spectroscopy was used to analyze the bare composite materials’ substrates and the DNA-contaminated samples. To the best of our knowledge, the evaluation of PET packaging contamination with DNA species by FTIR has not been reported previously. This study demonstrated that FTIR spectroscopy could provide a rapid, sensitive, and reliable approach for screening of biochemical contaminants on composite materials based on recycled PET.
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Research context
OpenAlex topics: Microplastics and Plastic Pollution · Recycling and Waste Management Techniques · Effects and risks of endocrine disrupting chemicals
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Advanced Research and Development Center for Experimental Medicine (CEMEX), Grigore T. Popa University of Medicine and Pharmacy of Iasi, 9-13 M. Kogalniceanu Street, 700454 Iasi, Romania
Department of Internal Medicine, Grigore T. Popa University of Medicine and Pharmacy of Iasi, 16 University Street, 700115 Iasi, Romania
Internal Medicine Clinic, St. Spiridon County Clinical Emergency Hospital, 1 Independentei Bd., 700111 Iasi, Romania
Electrical Engineering Faculty, Gheorghe Asachi Technical University of Iasi, 67 Prof. Dimitrie Mangeron Bd., 700050 Iasi, Romania
ALL GREEN SRL, 8 G. Cosbuc Street, 700470 Iasi, Romania
Funding
Ministry of Research, Innovation and Digitization, Romania · Grigore T. Popa University of Medicine and Pharmacy from Iasi, Romania
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