Comparative Studies on the Antioxidant, Antifungal, and Wound Healing Activities of Solenostemma arghel Ethyl Acetate and Methanolic Extracts
Source abstract
Abstract
Various herbal compounds are used for medical purposes due to their safety, as there are no or minimal side effects. This study was performed to assess the wound healing and antioxidant activities of ethyl acetate (EtOAc) and methanolic extract (MeoH) of Solenostemma arghel (S. arghel). Their antifungal activities were also evaluated against isolated swabs of equine wounds. They underwent GC-MS analysis for the characterization of both extracts. For wound healing evaluation, forty-five male albino rats were divided into three groups; the control group was treated with normal saline, and the other two groups were treated with S. arghel EtOAc and MeoH extract gels, respectively. The wounds were examined clinicopathologically and immunohistochemistry on the 3rd, 7th, and 14th days post-wounding. GC-Ms analysis of S. arghel recorded fifty-one volatile organic compounds (VOCs) within EtOAc extraction and thirty VOCs in MeoH extract. VOCs represented in EtOAc extract showed higher antioxidant activity and better and faster wound healing than VOCs of MeOH extract. The treated groups showed improved wound healing clinically and pathologically in comparison with the control group as they decreased the wound surface area (WSA) and percent (WSA%) and increased the wound contraction percent (WC%), epithelization, fibroblast proliferation with neovascularization, and reduced the inflammatory reaction. Moreover, the treated groups showed higher expression of vascular endothelial growth factor (VEGF) compared with the control. The EtOAc extract showed higher antifungal activity against Penicillium funiculosum, P. jensenii, M. cinctum, and Candida albicans, which were isolated from infected clinical equine wounds, than MeOH extract. The treated groups showed improved wound healing clinically and pathologically in comparison with the control group as they decreased the wound surface area (WSA) and percent (WSA%) and increased the wound contraction percent (WC%), epithelization, fibroblast proliferation with neovascularization, and reduced the inflammatory reaction. Moreover, the treated groups showed higher expression of vascular endothelial growth factor (VEGF) compared with the control. Additionally, the two extract gels showed promising healing of equine wounds. In conclusion, the study recommended the use of S. arghel EtOAc extract as it was proven to promote wound healing compared with MeoH extract.
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Research context
OpenAlex topics: Insect Pest Control Strategies · Essential Oils and Antimicrobial Activity · Beetle Biology and Toxicology Studies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Botany Department, Faculty of Science, Aswan University, Aswan 81528, Egypt
Unit of Environmental Studies and Development, Aswan University, Aswan 81528, Egypt
Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt
Department of Clinical Sciences, College of Medicine, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia
Department of Pharmacology, Faculty of Veterinary Medicine, Menoufia University, Shebin Elkoum 32514, Egypt
Department of Anatomy and Embryology, Faculty of Medicine, Benha University, Benha 13518, Egypt
Department of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Aswan University, Aswan 81528, Egypt
References (75)
Show source-provided references
1. Evaluation of the Wound Healing Activity of an Ethanolic Extract of Ceylon Cinnamon in Mice10.17221/4972-VETMED ↗
2. Aerobic, Anaerobic and Fungal Burn Wound Infections10.1016/S0195-6701(97)90148-1 ↗
3. The Microbiology of Acute and Chronic Wounds
4. Bacterial Isolates from Infected Wounds and Their Antibiotic Susceptibility Pattern: Some Remarks about Wound Infection10.1111/iwj.12049 ↗
5. A Proactive Approach to Wound Infection10.7748/ns2001.04.15.30.50.c3012 ↗
6. Redefining the Chronic-Wound Microbiome: Fungal Communities Are Prevalent, Dynamic, and Associated with Delayed Healing10.1128/mBio.01058-16 ↗
7. Survey of Fungi and Yeast in Polymicrobial Infections in Chronic Wounds10.12968/jowc.2011.20.1.40 ↗
8. Fungal Wound Infections: Mini Review
9. In Vivo Modeling of Biofilm-Infected Wounds: A Review10.1016/j.jss.2012.06.048 ↗
10. Normal Microscopic Anatomy of Equine Body and Limb Skin: A Morphological and Immunohistochemical Study10.1016/j.aanat.2018.03.010 ↗
11. Role of Collagen Cross-Linking on Equine Wound Contraction and Healing10.1007/s00580-008-0789-0 ↗
12. Aberrant Wound Healing in the Horse: Naturally Occurring Conditions Reminiscent of Those Observed in Man10.1111/wrr.12018 ↗
13. An Equine Wound Model to Study Effects of Bacterial Aggregates on Wound Healing10.1089/wound.2018.0901 ↗
14. Microbiology of Equine Wounds and Evidence of Bacterial Biofilms10.1016/j.vetmic.2011.01.003 ↗
15. The Most Common Herbal Remedies in Dongola Province, Northern Sudan
16. Monoterpene and Pregnane Glucosides from Solenostemma Argel10.1016/S0031-9422(99)00447-1 ↗
17. Pregnene Derivatives from Solenostemma Argel Leaves10.1016/S0031-9422(01)00121-2 ↗
18. Chemical Composition, Minerals, Protein Fractionation, and Anti-Nutrition Factors in Leaf of Hargel Plant (Solenostemma argel)
19. Influence of Argel (Solenostemma argel Del. Hayne) Soil Applications on Flowering and Yield of Date Palm (Phoenix dactylifera L.)10.5251/abjna.2011.2.3.538.542 ↗
20. New Pregnane and Phenolic Glycosides from Solenostemma Argel10.1016/j.fitote.2016.08.002 ↗
21. Endophytic Fungi from the Medicinal Herb Euphorbia Geniculata as a Potential Source for Bioactive Metabolites10.1007/s00203-019-01740-x ↗
22. Antibacterial and Antifungal Study of Some Essential Oils and Some of Their Constituents
23. Methods for in Vitro Evaluating Antimicrobial Activity: A Review10.1016/j.jpha.2015.11.005 ↗
24. Inhibition of Storage Fungi of Blackgram (Vigna mungo L.) by Some Essential Oils10.1002/(SICI)1099-1026(199901/02)14:1<1::AID-FFJ735>3.0.CO;2-R ↗
25. Wound-Healing Properties of Green (Using Lawsonia inermis Leaf Extract) and Chemically Synthesized Zno Nanoparticles in Albino Rats10.1007/s11356-021-17670-5 ↗
26. Healing Potential of Cream Containing Extract of Sphaeranthus Indicus on Dermal Wounds in Guinea Pigs10.1016/j.jep.2006.02.022 ↗
27. Healing Potential of Spirulina Platensis for Skin Wounds by Modulating BFGF, VEGF, TGF-SS1 and α-SMA Genes Expression Targeting Angiogenesis and Scar Tissue Formation in the Rat Model10.1016/j.biopha.2021.111349 ↗
28. Tissue Engineering in Wound Repair: The Three “R” s—Repair, Replace, Regenerate10.1111/j.1532-950X.2009.00585.x ↗
29. Antimicrobial and Antioxidant Screening of Curcumin and Pyrocatechol in the Prevention of Biodiesel Degradation: Oxidative Stability10.1080/17597269.2016.1168023 ↗
30. Mechanisms of Pathogenic Candida Species to Evade the Host Complement Attack10.3389/fcimb.2020.00094 ↗
31. Antifungal Activity of Ficus sycomorus L. Extracts against Dermatophytes and Other Associated Fungi Isolated from Camels Ringworm Lesions10.62400/jbs.v1i3.25 ↗
32. 17-Octadecynoic Acid Improves Contractile Response to Angiotensin II by Releasing Vasocontrictor Prostaglandins10.1016/j.prostaglandins.2011.07.008 ↗
33. A Review of Bioactivity Guided Medicinal Uses and Therapeutic Potentials of Noxious Weed (Alternanthera sessilis)
34. Determination of Bioactive Compounds of Methanolic Extract of Vitis Vinifera Using GC-MS
35. GC-MS Analysis of Phytochemical Constituents in Methanol Extract of Wood Bark from Durio Zibethinus Murr
36. Identification of N-Hexane Fraction Constituents of Archidium Ohioense (Schimp. Ex Mull) Extract Using GC-MS Technique
37. 2,4-Di-Tert-Butyl Phenol as the Antifungal, Antioxidant Bioactive Purified from a Newly Isolated Lactococcus sp.10.1016/j.ijfoodmicro.2015.06.025 ↗
38. Design and Elevation of Sex-attractant Traps for Pea Moth, Cydia Nigricana (Steph.) and the Effect of Plume Shape on Catches10.1111/j.1365-2311.1976.tb01221.x ↗
39. Inhibitory Effect of 1-tetradecanol on Helicobacter Pylori-induced Production of Interleukin-8 and Vascular Endothelial Growth Factor in Gastric Epithelial Cells10.3892/mmr.2017.7793 ↗
40. GC-MS Based Metabolite Profiling, Antioxidant and Antimicrobial Properties of Different Solvent Extracts of Malaysian Plectranthus Amboinicus Leaves10.1155/2017/1517683 ↗
41. Response to Heavy Metals in Organisms—I. Excretion and Accumulation of Physiological and Non Physiological Metals in Euglena Gracilis10.1016/0306-4492(80)90006-4 ↗
42. Investigation of the Biological Activities and Characterization of Bioactive Constituents of Ophiorrhiza Rugosa Var. Prostrata (D. Don) & Mondal Leaves through in Vivo, in Vitro, and in Silico Approaches10.3390/molecules24071367 ↗
43. Phytochemical Analysis and Bioactivity Prediction of Compounds in Methanolic Extracts of Curculigo Orchioides Gaertn
44. Chemical Constituents of Methanol Leaf Extract of Aspilia Africana CD Adams by GC MS
45. The Gc Ms Analysis of Ethyl Acetate Extract of One Herbal Plant, ‘Jatrophacurcus’
46. Copper as a Cofactor and Regulator of Copper, Zinc Superoxide Dismutase10.1093/jn/122.suppl_3.636 ↗
47. GC-MS Analysis of Phytocomponents in Pleiospermium Alatum (Wall. Ex Wight & Arn.) Swingle, (Rutaceae)
48. Analysis of Bioactive Chemical Compounds of Euphorbia Lathyrus Using Gas Chromatography-Mass Spectrometry and Fourier-Transform Infrared Spectroscopy
49. Identification of bioactive compounds in flower of Tabernaemontana divaricata (L.) using gas chromatography–mass spectrometry analysis10.22159/ajpcr.2019.v12i9.34559 ↗
50. Phthalate Derivatives from Marine Penicillium Decumbens and Its Synergetic Effect against Sepsis Bacteria10.33263/BRIAC94.070076 ↗
51. Anti-Nociceptive Activity of Ethnomedicinally Important Analgesic Plant Isodon Rugosus Wall. Ex Benth: Mechanistic Study and Identifications of Bioactive Compounds10.3389/fphar.2016.00200 ↗
52. Analgesic Activity of Peperomia Pellucida Aerial Parts in Mice10.1016/S0367-326X(00)00249-5 ↗
53. GC MS Determination of Bioactive Components of Peperomia pellucida (L.) Kunth
54. Arrigoni-Blank, M.d.F., Oliveira, R.L.B., Mendes, S.S., de Albuquerque Silva, P., Antoniolli, Â.R., Vilar, J.C., de Holanda Cavalcanti, S.C., and Blank, A.F. (2002). Seed Germination, Phenology, and Antiedematogenic Activity of Peperomia pellucida (L.) HBK. BMC Pharmacol., 2.10.1186/1471-2210-2-12 ↗
55. Potential Impacts of Pinus Halepensis Miller Trees as a Source of Phytochemical Compounds: Antibacterial Activity of the Cones Essential Oil and n-Butanol Extract10.1007/s10457-018-0324-5 ↗
56. Otieno, A.J. (2016). Antimicrobial Activity and Phytochemical Profiles of Warburgia Ugandensis Sprague (Canellaceae) Extracts from Different Populations across the Kenyan Rift Valley. [Ph.D. Thesis, Kenyatta University].
57. Kim, D.H., Park, M.H., Choi, Y.J., Chung, K.W., Park, C.H., Jang, E.J., An, H.J., Yu, B.P., and Chung, H.Y. (2013). Molecular Study of Dietary Heptadecane for the Anti-Inflammatory Modulation of NF-KB in the Aged Kidney. PLoS ONE, 8.10.1371/journal.pone.0059316 ↗
58. Wogonin, a Flavonoid Isolated from Scutellaria Baicalensis, Has Anti-Viral Activities against Influenza Infection via Modulation of AMPK Pathways10.4149/av_2018_109 ↗
59. Antiradical and Antioxidant Activity of Flavones from Scutellariae Baicalensis Radix10.1080/14786419.2014.983920 ↗
60. The Anticonvulsant and Neuroprotective Effects of Baicalin on Pilocarpine-Induced Epileptic Model in Rats10.1007/s11064-012-0771-8 ↗
61. Wogonoside Displays Anti-Inflammatory Effects through Modulating Inflammatory Mediator Expression Using RAW264. 7 Cells10.1016/j.jep.2013.04.025 ↗
62. Scutellaria Baicalensis Georgi Extract Protects against Alcohol-induced Acute Liver Injury in Mice and Affects the Mechanism of ER Stress10.3892/mmr.2016.4941 ↗
63. Essential Oil Composition of Two Scutellaria Species from Iran
64. Possible Anti-Tumor-Promoting Activity of Components in Japanese Soybean Fermented Food, Natto: Effect on Gap Junctional Intercellular Communication10.1093/carcin/16.3.471 ↗
65. Bioassay-Guided Isolation and Identification of Cytotoxic Compounds from Gymnosperma Glutinosum Leaves10.3390/molecules170911229 ↗
66. Phytopharmacological Review on Acanthospermum Hispidum
67. Antiinflammatory Effect of Oldenlandia Diffusa and Its Constituent, Hentriacontane, through Suppression of Caspase-1 Activation in Mouse Peritoneal Macrophages10.1002/ptr.3443 ↗
68. Anti-Inflammatory Potential of Hentriacontane in LPS Stimulated RAW 264.7 Cells and Mice Model10.1016/j.biopha.2017.05.063 ↗
69. GC-MS Analysis of Phytoconstituents in Alcohol Extract of Epiphyllum Oxypetalum Leaves
70. Preliminary Phytochemical Investigation, Antimicrobial Activity and GC-MS Analysis of Leaf Extract of Capparis Zeylanica Linn
71. Dibutyl Phthalate, the Bioactive Compound Produced by Streptomyces Albidoflavus 321.210.1016/j.micres.2005.06.007 ↗
72. Gastroprotective Effect of Mucilage Fraction from Solenostemma Argel via Cytoprotection and Attenuation of Oxidative Stress, Inflammation and Apoptosis10.34172/jhp.2021.26 ↗
73. Neutral Components in the Leaves and Seeds of Syzygium Cumini
74. Effect of Bandaging on Second Intention Healing of Wounds of the Distal Limb in Horses10.1111/j.1751-0813.2009.00428.x ↗
75. The Antimicrobial Activity of Honey against Common Equine Wound Bacterial Isolates10.1016/j.tvjl.2013.07.003 ↗