Puerarin Improves Dexamethasone-Impaired Wound Healing In Vitro and In Vivo by Enhancing Keratinocyte Proliferation and Migration
Source abstract
Abstract
The delayed and impaired wound healing caused by dexamethasone (DEX) is commonly reported. Puerarin, the major isoflavone found in Pueraria montana var. lobata (Willd.) Sanjappa & Pradeep promoted the wound healing process in diabetic rats. However, the effects and underlying mechanisms of puerarin on DEX-impaired wound healing have not been investigated. This study examined the potential uses of puerarin in upregulating keratinocyte proliferation and migration in dexamethasone (DEX)-suppressed wound healing model. The effects of puerarin on wound healing in vivo were investigated by taking full-thickness 5 mm punch biopsies from the dorsal skin of BALB/c mice and then treating them topically with 0.1% DEX. For the in vitro study, DEX-treated HaCaT cells were used to examine the effects of puerarin on DEX-induced keratinocyte proliferation and migration and the mechanisms of its action. Puerarin, when applied topically, accelerated the wound closure rate, increased the density of the capillaries, and upregulated the level of collagen fibers and TGF-β in the wound sites compared to the DEX-treated mice. Puerarin promoted the proliferation and migration of keratinocytes by activating the ERK and Akt signaling pathways in DEX-treated HaCaT cells. In conclusion, puerarin could be effective in reversing delayed and disrupted wound healing associated with DEX treatments.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Wound Healing and Treatments · Bioactive Compounds in Plants · Pressure Ulcer Prevention and Management
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Physiology, College of Korean Medicine, Dongguk University, Gyeongju 38066, Korea
Department of Anatomy, College of Korean Medicine, Semyung University, Jecheon 27136, Korea
Funding
Dongguk University
References (43)
Show source-provided references
1. Dexamethasone but not tacrolimus suppresses TNF-alpha-induced thymic stromal lymphopoietin expression in lesional keratinocytes of atopic dermatitis model10.1016/j.jdermsci.2015.06.016 ↗
2. Adverse effects of topical glucocorticosteroids10.1016/j.jaad.2005.01.010 ↗
3. The Wound Healing Process: An Overview of the Cellular and Molecular Mechanisms10.1177/147323000903700531 ↗
4. Cellular and molecular facets of keratinocyte reepithelization during wound healing10.1016/j.yexcr.2004.10.033 ↗
5. Glucocorticoids Inhibit Wound Healing: Novel Mechanism of Action10.1016/j.jid.2017.01.024 ↗
6. Pueraria montana var. lobata root extract inhibits photoaging on skin through Nrf2 pathway10.4014/jmb.1812.12019 ↗
7. A Promising View of Kudzu Plant, Pueraria montana var. lobata (Willd.) Sanjappa & Pradeep: Flavonoid Phytochemical Compounds, Taxonomic Data, Traditional Uses and Potential Biological Activities for Future Cosmetic Application10.3390/cosmetics7010012 ↗
8. Wound healing properties of flavonoids: A systematic review highlighting the mechanisms of action10.1016/j.phymed.2021.153636 ↗
9. Polydopamine/puerarin nanoparticle-incorporated hybrid hydrogels for enhanced wound healing10.1039/C9BM00991D ↗
10. Potential Skin Regeneration Activity and Chemical Composition of Absolute from Pueraria thunbergiana Flower
11. Isolation of radiolabeled isoflavones from kudzu (Pueraria lobata) root cultures10.1021/jf801413z ↗
12. Jung, H.W., Kang, A.N., Kang, S.Y., Park, Y.-K., and Song, M.Y. (2017). The root extract of Pueraria lobata and its main compound, puerarin, prevent obesity by increasing the energy metabolism in skeletal muscle. Nutrients, 9.10.3390/nu9010033 ↗
13. The suppressive effect of puerarin on atopic dermatitis-like skin lesions through regulation of inflammatory mediators in vitro and in vivo10.1016/j.bbrc.2018.03.018 ↗
14. Puerarin induces angiogenesis in myocardium of rat with myocardial infarction10.1248/bpb.29.945 ↗
15. Anti-inflammatory and antioxidant activities of constituents isolated from Pueraria lobata roots10.1007/s12272-012-0508-x ↗
16. The effect of oral puerarin administration on wound healing in diabetic rat model10.5455/annalsmedres.2018.05.074 ↗
17. Somboonwong, J., Kankaisre, M., Tantisira, B., and Tantisira, M.H. (2012). Wound healing activities of different extracts of Centella asiatica in incision and burn wound models: An experimental animal study. BMC Complement. Altern. Med., 12.10.1186/1472-6882-12-103 ↗
18. In vitro and In vivo wound healing studies of methanolic fraction of Centella asiatica extract10.1016/j.sajb.2016.10.022 ↗
19. Ruksiriwanich, W., Khantham, C., Sringarm, K., Sommano, S., and Jantrawut, P. (2020). Depigmented Centella asiatica Extraction by Pretreated with Supercritical Carbon Dioxide Fluid for Wound Healing Application. Processes, 8.10.3390/pr8030277 ↗
20. Effect of Centella asiatica L (Umbelliferae) on normal and dexamethasone-suppressed wound healing in Wistar Albino rats10.1177/1534734606291313 ↗
21. Skin wound healing model-excisional wounding and assessment of lesion area10.21769/BioProtoc.1661 ↗
22. The use of modified Masson’s trichrome staining in collagen evaluation in wound healing study
23. Gagamyeonryunggobon-dan (Jiājiǎnyánlínggùběn-dān) induces hair regrowth effect from activating hair follicle
24. Loss of CAR promotes migration and proliferation of HaCaT cells and accelerates wound healing in rats via Src-p38 MAPK pathway10.1038/srep19735 ↗
25. Asao, T., and Asaduzzaman, M. (2018). Role of Flavonoids as Wound Healing Agent. Phytochemicals-Source of Antioxidants and Role in Disease Prevention, IntechOpen.10.5772/intechopen.72985 ↗
26. Isoflavonoids as wound healing agents from Ononidis Radix10.1016/j.jep.2017.09.029 ↗
27. Centella asiatica in dermatology: An overview10.1002/ptr.5110 ↗
28. Induction of keratinocyte migration by ECa 233 is mediated through FAK/Akt, ERK, and p38 MAPK signaling10.1002/ptr.6075 ↗
29. Davison-Kotler, E., Marshall, W.S., and Garcia-Gareta, E. (2019). Sources of Collagen for Biomaterials in Skin Wound Healing. Bioengineering, 6.10.3390/bioengineering6030056 ↗
30. Chicharro-Alcantara, D., Rubio-Zaragoza, M., Damia-Gimenez, E., Carrillo-Poveda, J.M., Cuervo-Serrato, B., Pelaez-Gorrea, P., and Sopena-Juncosa, J.J. (2018). Platelet Rich Plasma: New Insights for Cutaneous Wound Healing Management. J. Funct. Biomater., 9.10.3390/jfb9010010 ↗
31. The role of the TGF-beta family in wound healing, burns and scarring: A review
32. Vascular Endothelial Growth Factor and Angiogenesis in the Regulation of Cutaneous Wound Repair10.1089/wound.2013.0517 ↗
33. Effects of steroids and retinoids on wound healing10.1001/archsurg.135.11.1265 ↗
34. Morgan, R., Keen, J., Halligan, D., O’Callaghan, A., Andrew, R., Livingstone, D., Abernethie, A., Maltese, G., Walker, B., and Hadoke, P. (2018). Species-specific regulation of angiogenesis by glucocorticoids reveals contrasting effects on inflammatory and angiogenic pathways. PLoS ONE, 13.10.1371/journal.pone.0192746 ↗
35. Role of keratinocytes in healing of chronic wounds
36. Neuman, T.S., and Thom, S.R. (2008). Oxygen and the basic mechanisms of wound healing. Physiology and Medicine of Hyperbaric Oxygen Therapy, Saunders.
37. Puerarin inhibits TRPM3/miR-204 to promote MC3T3-E1 cells proliferation, differentiation and mineralization10.1002/ptr.6034 ↗
38. Puerarin suppresses cell growth and migration in HPV-positive cervical cancer cells by inhibiting the PI3K/mTOR signaling pathway
39. Effects of puerarin on proliferation, apoptosis, migration and invasion of hormone-independent prostate cancer cells
40. AKT/PKB signaling: Navigating the network10.1016/j.cell.2017.04.001 ↗
41. Roles of the Raf/MEK/ERK pathway in cell growth, malignant transformation and drug resistance10.1016/j.bbamcr.2006.10.001 ↗
42. Estrogen accelerates cutaneous wound healing by promoting proliferation of epidermal keratinocytes via Erk/Akt signaling pathway10.1159/000443048 ↗
43. Icariin promotes wound healing by enhancing the migration and proliferation of keratinocytes via the AKT and ERK signaling pathway