Evaluation of Keratin/Bacterial Cellulose Based Scaffolds as Potential Burned Wound Dressing
Source abstract
Abstract
The study presents the preparation and characterization of new scaffolds based on bacterial cellulose and keratin hydrogel which were seeded with adipose stem cells. The bacterial cellulose was obtained by developing an Acetobacter xylinum culture and was visualized using SEM (scanning electron microscopy) and elementally determined through EDAX (dispersive X-ray analysis) tests. Keratin species (β–keratose and γ-keratose) was extracted by hydrolytic degradation from non-dyed human hair. SEM, EDAX and conductometric titration tests were performed for physical–chemical and morphological evaluation. Cytocompatibility tests performed in vitro confirmed the material non-toxic effect on cells. The scaffolds, with and without stem cells, were grafted on the burned wounds on the rabbit’s dorsal region and the grafts were monitored for 21 days after the application on the wounds. The clinical monitoring of the grafts and the histopathological examination demonstrated the regenerative potential of the bacterial cellulose–keratin scaffolds, under the test conditions.
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Research context
OpenAlex topics: Dyeing and Modifying Textile Fibers · Wound Healing and Treatments · Skin Protection and Aging
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Chemical Engineering, Faculty of Industrial Design and Affairs Management, “Gheorghe Asachi” Technical University Iasi, Mangeron Street, No/29, 700050 Iași, Romania
Department of Biomedical Sciences, Faculty of Medical Bioengineering, “Grigore T. Popa” University of Medicine and Pharmacy, 11-16, M. Kogalniceanu Street, No. 9-13, 700454 Iasi, Romania
Faculty of Agriculture, “Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, M. Sadoveanu Alley, No. 3, 700490 Iasi, Romania
Faculty of Veterinary Medicine, “Ion Ionescu de la Brad” University of Agricultural Sciences and Veterinary Medicine, M. Sadoveanu Alley, No. 8, 700489 Iasi, Romania
Department of Mechanical Engineering, Faculty of Mechanics, ”Gheorghe Asachi” Technical University Iasi, Mangeron Street, No. 29, 700050 Iași, Romania
Department of Environmental Engineering and Management, Cristofor Simionescu Faculty of Chemical Engineering and Environmental Protection, “Gheorghe Asachi” Technical University of Iasi, 700050 Iasi, Romania
Department of Plastic Surgery, “Grigore T. Popa”, University of Medicine and Pharmacy Iasi, Universitatii Street, No. 16, 700115 Iași, Romania
Department of General Surgery, “Grigore T. Popa”, University of Medicine and Pharmacy Iasi, Universitatii Street, No. 16, 700115 Iași, Romania
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