journal article·2022-11-17Open access

The Effect of LEDs on Biomass and Phycobiliproteins Production in Thermotolerant Oscillatoria sp.

Jefferson E. Contreras-Ropero, Valentina S. Lidueñez-Ballesteros, Angie D. Rodríguez-Bohórquez, Janet B. García-Martínez, Néstor A. Urbina-Suarez, Germán L. López-Barrera, Andrés F. Barajas-Solano, Samantha J. Bryan, Antonio Zuorro

Applied Sciences· Volume 12· Issue 22

Abstract

This study evaluates the role of different LED lights (white, blue/red), intensity (µmol m−2 s−1), and photoperiod in the production of biomass and phycocyanin-C, allophycocyanin and phycoerythrin (C-PC, APC, and PE respectively) from a novel thermotolerant strain of Oscillatoria sp. Results show that a mixture of white with blue/red LEDs can effectively double the biomass concentration up to 1.3 g/L, while the concentration of the selected phycobiliproteins increased proportionally to biomass. Results also indicate that high light intensities (>120 µmol m−2 s−1) can diminish the final concentration of C-PC, APC, and PE, significantly reducing the overall biomass produced. Finally, the photoperiod analysis showed that longer light exposure times (18:6 h) improved both biomass and phycobiliproteins concentration. These results demonstrate that the application of LEDs to produce a novel strain of Oscillatoria sp can double the biomass concentration, and the photoperiod regulation can eventually enhance the final concentration of specific phycobiliproteins such as APC and PE.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Algal biology and biofuel production · Light effects on plants · Photosynthetic Processes and Mechanisms

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Environmental Sciences, Universidad Francisco de Paula Santander, Av. Gran Colombia No. 12E-96, Cucuta 540003, Colombia

Department of Chemical and Environmental Engineering, University of Nottingham, Nottingham NG7 2RD, UK

Department of Chemical Engineering, Materials and Environment, Sapienza University, Via Eudossiana 18, 00184 Roma, Italy

Funding

Newton-Caldas Fund Institutional Links · Sapienza

References (75)

Show source-provided references
1. Enhancement of Phycobiliprotein Accumulation in Thermotolerant Oscillatoria Sp. through Media Optimization10.1021/acsomega.0c04665 ↗
2. Enhanced lipid extraction from unbroken microalgal cells using enzymes
3. Effect of Carbon-Nitrogen Ratio for the Biomass Production, Hydrocarbons and Lipids on Botryoccus braunii UIS 003
4. Evaluation of a Two-Phase Extraction System of Carbohydrates and Proteins from Chlorella vulgaris Utex 1803
5. Kinetic Modeling of Azo Dye Adsorption on Non-Living Cells of Nannochloropsis oceanica10.1016/j.jece.2017.07.078 ↗
6. Use of Cell Wall Degrading Enzymes to Improve the Recovery of Lipids from Chlorella sorokiniana10.1016/j.cej.2018.11.023 ↗
7. C-Phycocyanin Extraction from Two Freshwater Cyanobacteria by Freeze Thaw and Pulsed Electric Field Techniques to Improve Extraction Efficiency and Purity10.1016/j.algal.2020.101789 ↗
8. Effect of Air Temperature on Drying Kinetics and Quality Characteristics of Osmo-Treated Jumbo Squid (Dosidicus gigas)10.1016/j.lwt.2010.06.012 ↗
9. Stabilising Phycocyanin by Anionic Micelles10.1016/j.foodchem.2017.07.007 ↗
10. Antioxidant Potential of Nature’s “Something Blue”: Something New in the Marriage of Biological Activity and Extraction Methods Applied to C-Phycocyanin10.1016/j.tifs.2020.10.043 ↗
11. The Response of Phycobiliproteins to Light Qualities in Anabaena circinalis
12. Phycocyanin from Spirulina: A Review of Extraction Methods and Stability10.1016/j.foodres.2021.110314 ↗
13. Comparative Study of the Impact of Conventional and Unconventional Drying Processes on Phycobiliproteins from Arthrospira platensis10.1016/j.algal.2020.102165 ↗
14. Ultrasound-Assisted Enzymatic Extraction of Natural Food Colorant C-Phycocyanin from Dry Biomass of Arthrospira platensis10.1016/j.lwt.2019.108802 ↗
15. Design Tool and Guidelines for Outdoor Photobioreactors10.1016/j.ces.2013.11.014 ↗
16. Effect of Light and Temperature on the Cyanobacterium Arthronema africanum—A Prospective Phycobiliprotein-Producing Strain10.1007/s10811-007-9167-6 ↗
17. Accumulation of Phycocyanin in Heterotrophic and Mixotrophic Cultures of the Acidophilic Red Alga Galdieria sulphuraria10.1016/j.enzmictec.2005.05.010 ↗
18. Potential New Genera of Cyanobacterial Strains Isolated from Thermal Springs of Western Sichuan, China10.1016/j.algal.2018.01.008 ↗
19. Green Extraction of Value-Added Compounds Form Microalgae: A Short Review on Natural Deep Eutectic Solvents (NaDES) and Related Pre-Treatments10.1016/j.jece.2021.105989 ↗
20. Zuorro, A., García-Martínez, J.B., and Barajas-Solano, A.F. (2021). The Application of Catalytic Processes on the Production of Algae-Based Biofuels: A Review. Catalysts, 11.10.20944/preprints202008.0469.v1 ↗
21. Towards the Production of Microalgae Biofuels: The Effect of the Culture Medium on Lipid Deposition10.5114/bta.2019.87578 ↗
22. Quintero-Dallos, V., García-Martínez, J.B., Contreras-Ropero, J.E., Barajas-Solano, A.F., Barajas-Ferrerira, C., Lavecchia, R., and Zuorro, A. (2019). Vinasse as a Sustainable Medium for the Production of Chlorella vulgaris UTEX 1803. Water, 11.10.20944/preprints201906.0244.v1 ↗
23. Spirulina Cultivated under Different Light Emitting Diodes: Enhanced Cell Growth and Phycocyanin Production10.1016/j.biortech.2018.01.122 ↗
24. Light Factors and Nitrogen Availability to Enhance Biomass and C-Phycocyanin Productivity of Thermosynechococcus Sp. CL-110.1016/j.bej.2020.107899 ↗
25. Effect of Light Colour and Photoperiod on Biomass Growth and Phycocyanin Production by Synechococcus PCC 671510.1016/j.biortech.2020.123700 ↗
26. Combining Light Strategies with Recycled Medium to Enhance the Economic Feasibility of Phycocyanin Production with Spirulina platensis10.1016/j.biortech.2017.09.165 ↗
27. Repeated Fed-Batch Cultivation of Thermosynechococcus elongatus BP-1 in Flat-Panel Airlift Photobioreactors with Static Mixers for Improved Light Utilization: Influence of Nitrate, Carbon Supply and Photobioreactor Design10.1016/j.algal.2016.03.040 ↗
28. Stability and Antioxidant Activity of Food-Grade Phycocyanin Isolated from Spirulina Platensis10.1080/10942912.2015.1038564 ↗
29. Effects of Different Light Source and Media on Growth and Production of Phycobiliprotein from Freshwater Cyanobacteria10.1016/j.biortech.2017.10.052 ↗
30. Contrasting Effects of Monochromatic LED Lighting on Growth, Pigments and Photosynthesis in the Commercially Important Cyanobacterium Arthrospira maxima10.1016/j.biortech.2019.121846 ↗
31. Blue Light Reduces Photosynthetic Efficiency of Cyanobacteria through an Imbalance between Photosystems I and II10.1007/s11120-018-0561-5 ↗
32. Cultivation of Microalgae on Artificial Light Comes at a Cost10.1016/j.algal.2013.09.004 ↗
33. Light Emitting Diodes (LEDs) Applied to Microalgal Production10.1016/j.tibtech.2014.06.001 ↗
34. Effects of Various LED Light Wavelengths and Intensities on Microalgae-Based Simultaneous Biogas Upgrading and Digestate Nutrient Reduction Process10.1016/j.biortech.2013.03.051 ↗
35. Enhancing Growth and Lipid Production of Marine Microalgae for Biodiesel Production via the Use of Different LED Wavelengths10.1016/j.biortech.2014.03.113 ↗
36. Assunção, J., Pagels, F., Tavares, T., Malcata, F.X., and Guedes, A.C. (2022). Light Modulation for Bioactive Pigment Production in Synechocystis Salina. Bioengineering, 9.10.3390/bioengineering9070331 ↗
37. Okamoto, A., Imamura, M., Tani, K., and Matsumoto, T. (2021). The Effect of Using Light Emitting Diodes and Fluorescent Lamps as Different Light Sources in Growth Inhibition Tests of Green Alga, Diatom, and Cyanobacteria. PLoS ONE, 16.10.1371/journal.pone.0247426 ↗
38. Mehariya, S., Goswami, R.K., Verma, P., Lavecchia, R., and Zuorro, A. (2021). Integrated Approach for Wastewater Treatment and Biofuel Production in Microalgae Biorefineries. Energies, 14.10.3390/en14082282 ↗
39. Pigment Productions by Spirulina Platensis as a Renewable Resource
40. The Production of High Phycocyanin by Applications of Red Light-Emitting Diodes (LEDs) In Vitro Algae Growth on Spirulina platensis10.21272/jnep.13(3).03034 ↗
41. The Effect of Temperature and Enzyme Concentration in the Transesterification Process of Synthetic Microalgae Oil
42. Performance of the Mixed LED Light Quality on the Growth and Energy Efficiency of Arthrospira platensis10.1007/s00253-018-8923-7 ↗
43. Effects of Light Quality and Temperature on the Photosynthesis and Pigment Content of a Subtidal Edible Red Alga Meristotheca papulosa (Solieriaceae, Gigartinales) from Japan10.1007/s10811-020-02045-z ↗
44. Effect Of Thermal Pre-Treatment On Fermentable Sugar Production Of Chlorella vulgaris
45. White and Red LEDs as Two-Phase Batch for Cyanobacterial Pigments Production10.1016/j.biortech.2020.123105 ↗
46. Urbina-Suarez, N.A., Ayala-González, D.D., Rivera-Amaya, J.D., Barajas-Solano, A.F., and Machuca-Martínez, F. (2022). Evaluation of the Light/Dark Cycle and Concentration of Tannery Wastewater in the Production of Biomass and Metabolites of Industrial Interest from Microalgae and Cyanobacteria. Water, 14.10.3390/w14030346 ↗
47. Automation of Pilot-Scale Open Raceway Pond: A Case Study of CO2-Fed PH Control on Spirulina Biomass, Protein and Phycocyanin Production10.1016/j.jcou.2019.07.006 ↗
48. The Production of Biomass and Phycobiliprotein Pigments in Filamentous Cyanobacteria: The Impact of Light and Carbon Sources10.1134/S000368381705009X ↗
49. A Novel Two-Phase Bioprocess for the Production of Arthrospira (Spirulina) maxima LJGR1 at Pilot Plant Scale during Different Seasons and for Phycocyanin Induction under Controlled Conditions10.1016/j.biortech.2019.122548 ↗
50. Milia, M., Corrias, F., Addis, P., Zitelli, G.C., Cicchi, B., Torzillo, G., Andreotti, V., and Angioni, A. (2022). Influence of Different Light Sources on the Biochemical Composition of Arthrospira Spp. Grown in Model Systems. Foods, 11.10.3390/foods11030399 ↗
51. Effect of Various Light Spectra on Amino Acids and Pigment Production of Arthrospira platensis Using Flat-Plate Photobioreactor10.1080/10826068.2021.1941102 ↗
52. Szwarc, D., and Zieliński, M. (2018). Effect of Lighting on the Intensification of Phycocyanin Production in a Culture of Arthrospira platensis. Proceedings, 2.10.3390/proceedings2201305 ↗
53. Internally Illuminated Photobioreactor Using a Novel Type of Light-Emitting Diode (LED) Bar for Cultivation of Arthrospira platensis10.1007/s12257-016-0428-6 ↗
54. Enhanced Phycocyanin Production from Spirulina platensis Using Light Emitting Diode10.1007/s40034-016-0090-8 ↗
55. Fed-Batch Strategy for Enhancing Cell Growth and C-Phycocyanin Production of Arthrospira (Spirulina) platensis under Phototrophic Cultivation10.1016/j.biortech.2014.12.073 ↗
56. Study of Phycocyanin Production from Spirulina platensis Under Different Light Spectra10.1590/S1516-89132011000400005 ↗
57. Effect of Various Colors of Light-Emitting Diodes (LEDs) on the Biomass Composition of Arthrospira platensis Cultivated in Semi-Continuous Mode10.1007/s12010-014-0727-3 ↗
58. Modeling on Chlorophyll a and Phycocyanin Production by Spirulina platensis under Various Light-Emitting Diodes10.1016/j.bej.2010.09.004 ↗
59. The Production of High Purity Phycocyanin by Spirulina platensis Using Light-Emitting Diodes Based Two-Stage Cultivation10.1007/s12010-015-1879-5 ↗
60. Efficacy of Red Light for Enhanced Cell Disruption and Fluorescence Intensity of Phycocyanin10.1007/s00449-020-02430-5 ↗
61. Influence of Light Intensity and Photoperiod on Energy Efficiency of Biomass and Pigment Production of Spirulina (Arthrospira platensis)
62. Silkina, A., Kultschar, B., and Llewellyn, C.A. (2019). Far-Red Light Acclimation for Improved Mass Cultivation of Cyanobacteria. Metabolites, 9.10.3390/metabo9080170 ↗
63. Growth and Pigment Content of Gracilaria tikvahiae McLachlan under Fluorescent and LED Lighting10.1016/j.aquaculture.2014.10.037 ↗
64. Optimization of Porphyridium purpureum Culture Growth Using Two Variables Experimental Design: Light and Sodium Bicarbonate
65. Barajas-Solano, A.F. (2022). Optimization of Phycobiliprotein Solubilization from a Thermotolerant Oscillatoria Sp.. Processes, 10.10.3390/pr10050836 ↗
66. Andersen, R.A. (2005). Appendix A—Recipes for Freshwater and Seawater Media. Algal Culturing Techniques, Elsevier Academic Press.10.1016/B978-012088426-1/50027-5 ↗
67. Complementary Chromatic Adaptation in a Filamentous Blue-Green Alga10.1083/jcb.58.2.419 ↗
68. Method to Obtain C-Phycocyanin of High Purity10.1016/j.chroma.2006.05.073 ↗
69. Extraction and Purification of C-Phycocyanin from Spirulina platensis in Conventional and Integrated Aqueous Two-Phase Systems10.1590/S0103-50532010000500022 ↗
70. Borowitzka, M.A., and Moheimani, N.R. (2013). Standard Methods for Measuring Growth of Algae and Their Composition BT—Algae for Biofuels and Energy, Springer Netherlands.10.1007/978-94-007-5479-9 ↗
71. Phycocyanin Production in Seawater Culture of Arthrospira maxima10.7773/cm.v26i4.619 ↗
72. Outdoor Scale-up of Leptolyngbya Sp.: Effect of Light Intensity and Inoculum Volume on Photoinhibition and -Oxidation10.1002/bit.27750 ↗
73. Jung, C.H.G., Waldeck, P., Sykora, S., Braune, S., Petrick, I., Küpper, J.-H., and Jung, F. (2022). Influence of Different Light-Emitting Diode Colors on Growth and Phycobiliprotein Generation of Arthrospira platensis. Life, 12.10.3390/life12060895 ↗
74. Hotos, G.N., and Antoniadis, T.I. (2022). The Effect of Colored and White Light on Growth and Phycobiliproteins, Chlorophyll and Carotenoids Content of the Marine Cyanobacteria Phormidium Sp. and Cyanothece Sp. in Batch Cultures. Life, 12.10.20944/preprints202201.0437.v1 ↗
75. Protein, Phycocyanin, and Polysaccharide Production by Arthrospira platensis Grown with LED Light in Annular Photobioreactors10.1007/s10811-022-02707-0 ↗