journal article·2024-01-18Open access

Blackcurrant Pomace as a Rich Source of Anthocyanins: Ultrasound-Assisted Extraction under Different Parameters

Bartosz Kruszewski, Emanuele Boselli

Applied Sciences· Volume 14· Issue 2

Abstract

The industry is currently trying to manage the waste generated during juice pressing. Berry pomace is an especially rich source of many bioactive compounds. Blackcurrant pomace is particularly valuable because of the large amount of extractable anthocyanins remaining in the fruit skin. The aim of the study was to evaluate the effect of ultrasound-assisted extraction (UAE) parameters on the content of anthocyanins and color parameters of blackcurrant pomace extracts. The pomace used for the study was very rich in anthocyanins—an average content of 853.2 mg/100 g fresh weight. The effect of temperature (25, 35, 45 °C), time (15, 30, 60 min) and material/solvent ratio (1:20 or 1:7) on extraction yield was analyzed. The extracts were obtained using two solvents: water–ethanol acidified with HCl and water acidified with citric acid. Either solvent type, as well as the other parameters of the extraction process, have an impact on the level of anthocyanins in the extracts. The lowest range of extraction yield (63–68%) was obtained for a 1:7 ratio with water–citric acid solvent. The highest range of extraction yield (74–93%) was obtained for a 1:7 ratio with water–ethanol solvent. The most efficient method was extraction in a water–ethanol solvent (50/50 v/v) acidified with HCl, at a 1:7 material/solvent ratio, at 35 °C, for 15 min, providing 93% process efficiency. A strong relationship (r > 0.991) was also found between anthocyanin content and color saturation (C*) or hue (h°) in the obtained extracts. Based on the E1% coloring strength results, all the extracts obtained can be considered strong colorants, especially those obtained with the water–citric acid solvent. Ultrasonic extraction can be successfully applied to extract pigments from blackcurrant pomace and obtain, for example, food coloring.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Phytochemicals and Antioxidant Activities · Antioxidant Activity and Oxidative Stress · Microbial Metabolites in Food Biotechnology

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Food Technology and Assessment, Institute of Food Sciences, Warsaw University of Life Sciences—SGGW, 02-776 Warsaw, Poland

Faculty of Science and Technology, Free University of Bozen-Bolzano, Piazza Università 1, 39100 Bolzano, Italy

References (37)

Show source-provided references
1. Jurevičiūte, I., Keršiene, M., Bašinskiene, L., Leskauskaite, D., and Jasutiene, I. (2022). Characterization of Berry Pomace Powders as Dietary Fiber-Rich. Foods, 11.10.3390/foods11050716 ↗
2. Berry pomace—A review of processing and chemical analysis of its polyphenols10.1111/ijfs.13112 ↗
3. Tomato seed oil for edible use: Cold break, hot break, and harvest year effects10.1111/jfpp.13309 ↗
4. Multivariate analysis of structural and functional properties of fibres from apple pomace using different extraction methods10.1186/s43014-022-00119-8 ↗
5. Kidoń, M., Marciszak, E., Uğur, Ş., Kuligowski, M., and Radziejewska-Kubzdela, E. (2023). Chokeberry Pomace Utilization for Improving Selected Quality Parameters of Green Tea Leaves or Hibiscus Flower Infusions. Appl. Sci., 13.10.3390/app13148186 ↗
6. Exploiting blackcurrant juice press residue in extruded snacks10.1016/j.lwt.2014.02.005 ↗
7. Conversion of apple pomace waste to ethanol at industrial relevant conditions10.1007/s00253-016-7665-7 ↗
8. Ampese, L.C., Sganzerla, W.G., Di Domenico Ziero, H., Costa, J.M., Martins, G., and Forster-Carneiro, T. (2022). Valorization of apple pomace for biogas production: A leading anaerobic biorefinery approach for a circular bioeconomy. Biomass Convers. Biorefin.10.1007/s13399-022-03534-6 ↗
9. Bio-synthesis of citric acid from single and co-culture-based fermentation technology using agro-wastes
10. Extraction and antibacterial activity of Pulicaria gnaphalodes as a natural colorant: Characterization and application on wool fibers
11. Blackcurrants (Ribes nigrum): A Review on Chemistry, Processing, and Health Benefits10.1111/1750-3841.14781 ↗
12. High variability in flavonoid contents and composition between different North-European currant (Ribes spp.) varieties10.1016/j.foodchem.2016.02.056 ↗
13. Biological activities, therapeutic potential, and pharmacological aspects of blackcurrants (Ribes nigrum L): A comprehensive review10.1002/fsn3.3592 ↗
14. Acetic acid buffer as extraction medium for free and bound phenolics from dried blackcurrant (Ribes nigrum L.) skins10.1111/1750-3841.15466 ↗
15. Ultrasound assisted extraction (UAE) of bioactive compounds from fruit and vegetable processing by-products: A review10.1016/j.ultsonch.2020.105325 ↗
16. A comparison study between ultrasound–assisted and enzyme–assisted extraction of anthocyanins from blackcurrant (Ribes nigrum L.)10.1016/j.fochx.2021.100192 ↗
17. Effects of ecological factors on the antioxidant potential and total phenol content of Scrophularia striata Boiss10.1038/s41598-019-52605-8 ↗
18. Moradi, H., Ghavam, M., and Tavili, A. (2020). Study of antioxidant activity and some herbal compounds of Dracocephalum kotschyi Boiss. in different ages of growth. Biotechnol. Rep., 25.10.1016/j.btre.2019.e00408 ↗
19. Ultrasound-Assisted Extraction of Natural Pigments From Food Processing By-Products: A Review10.3389/fnut.2022.891462 ↗
20. Effect of acidified water on phenolic profile and antioxidant activity of dried blackcurrant (Ribes nigrum L.) pomace extracts10.1016/j.lwt.2021.112733 ↗
21. Microwave-Assisted Extraction and Ultrasound-Assisted Extraction of Bioactive Compounds from Grape Pomace
22. Ultrasound extraction of phenolics and anthocyanins from jabuticaba peel10.1016/j.indcrop.2015.02.059 ↗
23. Enzyme-assisted extraction of bioactive non-extractable polyphenols from sweet cherry (Prunus avium L.) pomace10.1016/j.foodchem.2020.128086 ↗
24. Biorefining of blackcurrant pomace into high value functional ingredients using supercritical CO2, pressurized liquid and enzyme assisted extractions10.1016/j.supflu.2017.01.003 ↗
25. Optimization of Pulsed Electric Field Treatment for the Extraction of Bioactive Compounds from Blackcurrant10.1007/s11947-019-02283-1 ↗
26. (2023). Official Methods of Analysis of AOAC International, AOAC International. [22nd ed.]. Official Method 920.152 Protein in Fruit Products Kjeldahl Method.
27. Kruszewski, B., Zawada, K., and Karpiński, P. (2021). Impact of High-Pressure Homogenization Parameters on Physicochemical Characteristics, Bioactive Compounds Content, and Antioxidant Capacity of Blackcurrant Juice. Molecules, 26.10.3390/molecules26061802 ↗
28. Anthocyanic pigment determination in red fruit juices, concentrated juices and syrups using liquid chromatography10.1016/S0003-2670(98)00756-9 ↗
29. Composition and physicochemical properties of dried berry pomace10.1002/jsfa.9302 ↗
30. Composition of industrial seedless black currant pomace10.1007/s00217-008-0968-x ↗
31. Ali Redha, A., Anusha Siddiqui, S., Zare, R., Spadaccini, D., Guazzotti, S., Feng, X., Bahmid, N.A., Wu, Y.S., Ozeer, F.Z., and Aluko, R.E. (2022). Blackcurrants: A Nutrient-Rich Source for the Development of Functional Foods for Improved Athletic Performance. Food Rev. Int.10.1080/87559129.2022.2162076 ↗
32. Šimerdová, B., Bobríková, M., Lhotská, I., Kaplan, J., Křenová, A., and Šatínský, D. (2021). Evaluation of anthocyanin profiles in various blackcurrant cultivars over a three-year period using a fast hplc-dad method. Foods, 10.10.3390/foods10081745 ↗
33. Ultrasound assisted citric acid mediated pectin extraction from industrial waste of Musa balbisiana10.1016/j.ultsonch.2016.09.019 ↗
34. Development and validation of ultrasound-assisted solid-liquid extraction of phenolic compounds from waste spent coffee grounds10.1016/j.ultsonch.2016.05.005 ↗
35. Assessment of the color modulation and stability of naturally copigmented anthocyanin-grape colorants with different levels of purification10.1016/j.foodres.2018.01.057 ↗
36. Chemistry and biochemistry of dietary polyphenols10.3390/nu2121231 ↗
37. Anthocyanins and derivatives are more than flavylium cations10.1016/j.tet.2014.09.051 ↗