Total Phenolic, Anthocyanins HPLC-DAD-MS Determination and Antioxidant Capacity in Black Grape Skins and Blackberries: A Comparative Study
Source abstract
Abstract
Anthocyanins are flavonoids with an antioxidant effect. They are the pigments that give rich colours to berries, red onions, pomegranates, and grapes. In addition to acting as antioxidants and fighting free radicals, anthocyanins may offer anti-inflammatory, anti-viral, and anti-cancer benefits. Among various types of fruits, blackberries and grapes are distinguished by their rich content in polyphenols, including anthocyanins. The purpose of this study was the identification and quantification of the anthocyanins in black grape skins and blackberries, but also the determination of the total phenolic content and total antioxidant capacity. The grape skins and blackberry extracts were prepared by an ultrasound-assisted acidified ethanol and methanol extraction method, with the 80% methanol solution being the most effective. Alcoholic extracts of blackberries and grape skins were analysed by the HPLC-DAD-MS method. There were five glycosylated anthocyanin compounds in blackberries, eight glycosylated anthocyanins compounds, and seven fragments of anthocyanin derivatives in grape skins identified. It was concluded that the anthocyanin profile of blackberries and grapes revealed mainly anthocyanin monoglycosides and acetylglycosides. Cyanidin-3-glucoside was the main component (86.49%) in blackberries, while, in the grape skins, the main component was delphinidin-3-O-glucoside (about 40.64%). Principal component analysis (PCA) was carried out on the basis of the 13 identified compounds in order to separate the extracts and describe the anthocyanins characteristics of different groups, the findings being in agreement with the experimental results. Compared to methanol extracts, ethanol extracts showed higher antioxidant activity, being related to the total phenolic content for the blackberries. Overall, the obtained results indicated that the blackberries and grapes skins possessed a high antioxidant content, similar to other berries, highlighting their potential use as fresh functional foods or fruit-derived products.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Phytochemicals and Antioxidant Activities · Fermentation and Sensory Analysis · Plant Gene Expression Analysis
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
National Research and Development Institute for Cryogenic and Isotopic Technologies—ICSI Ramnicu Valcea, 4th Uzinei Street, 240050 Ramnicu Valcea, Romania
Funding
Romanian Ministry of Research, Innovation and Digitalization
References (43)
Show source-provided references
1. Polyphenols as Antitumor Agents Targeting Key Players in Cancer-Driving Signaling Pathways10.3389/fphar.2021.710304 ↗
2. Polyphenols in cancer prevention: New insights (Review)10.3892/ijfn.2020.9 ↗
3. Polyphenols: Immunomodulatory and Therapeutic Implication in Colorectal Cancer10.3389/fimmu.2019.00729 ↗
4. The Role of Polyphenols in Human Health and Food Systems: A Mini-Review10.3389/fnut.2018.00087 ↗
5. Yahfoufi, N., Alsadi, N., Jambi, M., and Matar, C. (2018). The Immunomodulatory and Anti-Inflammatory Role of Polyphenols. Nutrients, 10.10.3390/nu10111618 ↗
6. Oreopoulou, A., Tsimogiannis, D., and Oreopoulou, V. (2019). Extraction of Polyphenols From Aromatic and Medicinal Plants: An Overview of the Methods and the Effect of Extraction Parameters. Polyphen. Plants, 243–259.10.1016/B978-0-12-813768-0.00025-6 ↗
7. Techniques and modeling of polyphenol extraction from food: A review10.1007/s10311-021-01217-8 ↗
8. Efficiency of Polyphenol Extraction from Artificial Honey Using C 18 Cartridges and Amberlite® XAD-2 Resin: A Comparative Study
9. Kammerer, D. (2016). Anthocyanins. Handbook on Natural Pigments in Food and Beverages: Industrial Applications for Improving Food Color, Woodhead Publishing.10.1016/B978-0-08-100371-8.00003-8 ↗
10. Anthocyanidins and anthocyanins: Colored pigments as food, pharmaceutical ingredients, and the potential health benefits10.1080/16546628.2017.1361779 ↗
11. De Pascual-Teresa, S., Sánchez-Ballesta, M.T., Garcia-Viguera, C., and Pascual-Teresa, S. (2013). Anthocyanins. Natural Products: Phytochemistry, Botany and Metabolism of Alkaloids, Phenolics and Terpenes, Springer.10.1007/978-3-642-22144-6_59 ↗
12. Paun, N., Niculescu, V., Tamaian, R., and Miricioiu, M. (July, January 29). Functionalized Mesoporous Silica Nanoparticles as Novel Systems for Natural Anthocyanins Stability Enhancement. Proceedings of the International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, Albena, Bulgary.
13. High concentrations of aromatic acylated anthocyanins found in cauline hairs in Plectranthus ciliates10.1016/j.phytochem.2016.04.007 ↗
14. Flamini, R., Mattivi, F., De Rosso, M., Arapitsas, P., and Bavaresco, L. (2013). Advanced Knowledge of Three Important Classes of Grape Phenolics: Anthocyanins, Stilbenes and Flavonols. Int. J. Mol. Sci., 14.10.3390/ijms141019651 ↗
15. Jordao, A.M., and Cosme, F. (2018). The Evolution of Polyphenols from Grapes to Wines. Grapes and Wines-Advances in Production, Processing, Analysis and Valorization, IntechOpen.10.5772/68059 ↗
16. Anthocyanins and their gut metabolites attenuate monocyte adhesion and transendothelial migration through nutrigenomic mechanisms regulating endothelial cell permeability10.1016/j.freeradbiomed.2018.06.027 ↗
17. Anthocyanins of Fruits and Vegetables-Their Occurrence, Analysis and Role in Human Nutrition10.2478/v10032-008-0001-8 ↗
18. Mattioli, R., Francioso, A., Mosca, L., and Silva, P. (2020). Anthocyanins: A Comprehensive Review of Their Chemical Properties and Health Effects on Cardiovascular and Neurodegenerative Diseases. Molecules, 25.10.3390/molecules25173809 ↗
19. Bioactive and antioxidant characteristics of blackberry cultivars from East Anatolia10.3906/tar-1511-78 ↗
20. Chemical and antioxidant properties of cultivated and wild fragaria and rubus berries10.1111/j.1745-4557.2010.00360.x ↗
21. Classification of red wines using suitable markers coupled with multivariate statistic analysis10.1016/j.foodchem.2015.07.112 ↗
22. Characterization and Classification of Romanian Wines by Origin A chemometric approach based on some metals and phenolic composition10.37358/RC.19.11.7643 ↗
23. Editorial: Bioactive Compounds Biosynthesis and Metabolism in Fruit and Vegetables10.3389/fpls.2020.00129 ↗
24. Study of the impact of vine cultivation technology on the Feteasca Neagra wine phenolic composition and antioxidant properties
25. Donno, D., Mellano, M.G., De Biaggi, M., Riondato, I., Rakotoniaina, E.N., and Beccaro, G.L. (2018). New Findings in Prunus padus L. Fruits as a Source of Natural Compounds: Characterization of Metabolite Profiles and Preliminary Evaluation of Antioxidant Activity. Molecules, 23.10.3390/molecules23040725 ↗
26. Przybylska-Balcerek, A., Szablewski, T., Szwajkowska-Michałek, L., Świerk, D., Cegielska-Radziejewska, R., Krejpcio, Z., Suchowilska, E., Tomczyk, Ł., and Stuper-Szablewska, K. (2021). Sambucus Nigra Extracts–Natural Antioxidants and Antimicrobial Compounds. Molecules, 26.10.3390/molecules26102910 ↗
27. Hidalgo, G.-I., and Almajano, M.P. (2017). Red Fruits: Extraction of Antioxidants, Phenolic Content, and Radical Scavenging Determination: A Review. Antioxidants, 6.10.3390/antiox6010007 ↗
28. Liquid chromatography-mass spectrometry General principles and instrumentation10.1016/0021-9673(94)01198-N ↗
29. The Coupling of Gas and Liquid Chromatography with Mass Spectrometry10.1002/(SICI)1096-9888(199903)34:3<157::AID-JMS804>3.0.CO;2-4 ↗
30. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica10.1016/j.jfda.2013.11.001 ↗
31. Lamuela-Raventós, R.M. (2017). Folin-Ciocalteu method for the measurement of total phenolic content and antioxidant capacity. Meas. Antioxid. Act. Capacit., 107–117.10.1002/9781119135388.ch6 ↗
32. Giusti, M.M., and Wrolstad, R.E. (2001). Characterization and measurement of anthocyanins by UV-Visible spectroscopy. Curr. Prot. Food Anal. Chem., F1.2.1–F1.2.13.10.1002/0471142913.faf0102s00 ↗
33. Antioxidant activity applying an improved ABTS radical cation decolorization assay10.1016/S0891-5849(98)00315-3 ↗
34. Solvent extraction of anthocyanins from wine pomace10.1111/j.1365-2621.1980.tb07534.x ↗
35. An effective method for extracting anthocyanins from blueberry based on freeze-ultrasonic thawing technology10.1016/j.ultsonch.2020.105192 ↗
36. Optimization of ultrasound-assisted extraction of anthocyanins in red raspberries and identification of anthocyanins in extract using high-performance liquid chromatography–mass spectrometry10.1016/j.ultsonch.2006.12.011 ↗
37. The predictive value of the antioxidant capacity of structurally related flavonoids using the Trolox equivalent antioxidant capacity (TEAC) assay10.1016/S0308-8146(00)00092-3 ↗
38. Antioxidant Activities of Selected Berries and Their Free, Esterified, and Insoluble-Bound Phenolic Acid Contents10.3746/pnf.2018.23.1.35 ↗
39. Enhancement of antioxidant activity, total phenolic and flavonoid content of black soybeans by solid state fermentation with Bacillus subtilis BCRC 1471510.1016/j.fm.2009.11.002 ↗
40. Enhancement of the antioxidant capacity of chickpeas by solid state fermentation with Cordyceps militaris SN-1810.1016/j.jff.2014.06.008 ↗
41. Anthocyanins HPLC-DAD and MS Characterization, Total Phenolics, and Antioxidant Activity of Some Berries Extracts10.1080/00032719.2011.582550 ↗
42. Polyphenols, Antioxidant Activity and Anti-angiogenic Potential of Red and White Grapes
43. Contribution of Anthocyanin Composition to Total Antioxidant Capacity of Berries10.1007/s11130-015-0514-5 ↗