The Role of the Microalgae–Bacteria Consortium in Biomass Formation and Its Application in Wastewater Treatment Systems: A Comprehensive Review
Source abstract
Abstract
The optimization of wastewater treatment technologies using biological processes is no longer limited to improving the removal of organic matter and nutrients, as it is possible to reduce area and energy consumption, and recover value-added by-products. In this context, the microalgae–bacteria consortium is an alternative for reducing costs, as microalgae produce the oxygen required by bacteria to oxidize organic matter through photosynthesis. Additionally, it is possible to extract different by-products such as lipids, biofertilizers, biogas, alginate-type exopolymers, and others. Furthermore, bioflocculation occurs naturally through the adhesion of microalgae to the surface of bacterial flocs, without the addition of chemical products. This review discusses the main systems that utilize the microalgae–bacteria consortium, the metabolism of the microalgae–bacteria consortium, and its performance in removing organic matter and nutrients, as well as the effect of operating conditions on the physical properties of the biomass. Among the highlighted systems are sequencing batch and single-batch reactors, high-rate ponds, and continuous flow reactors. Among the systems discussed in this work, the sequential batch reactor configurations found better biomass formation and production of extracellular polymeric substances and the continuous flow reactors showed lower installation and operating costs. From this perspective, the potential for full-scale application of each system can be evaluated once the optimum operating conditions have been defined and the limitations of each system have been understood.
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Research context
OpenAlex topics: Algal biology and biofuel production · Wastewater Treatment and Nitrogen Removal · Microbial Fuel Cells and Bioremediation
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Fiber Materials and Environmental Technologies (FibEnTech) Research Unit, GeoBioSciences, GeoTechnologies and GeoEngineering (GeoBioTec) Research Unit, Department of Civil Engineering and Architecture, University of Beira Interior, 6201-001 Covilhã, Portugal
Laboratory of Environmental Sanitation, Department of Civil and Environmental Engineering, Federal University of Pernambuco, Av. Academica Helio Ramos, s/n, Cidade Universitária, Recife 50740-530, Brazil
FibEnTech, Department of Chemistry, University of Beira Interior, 6201-001 Covilhã, Portugal
References (100+)
Show source-provided references
1. A Somatic Coliphage Threshold Approach to Improve the Management of Activated Sludge Wastewater Treatment Plant Effluents in Resource-Limited Regions10.1128/AEM.00616-20 ↗
2. A Review on Anaerobic-Aerobic Treatment of Industrial and Municipal Wastewater10.1016/j.cej.2009.06.041 ↗
3. Partial Nitrification-Anammox (PNA) Treating Sewage with Intermittent Aeration Mode: Effect of Influent C/N Ratios10.1016/j.cej.2017.10.072 ↗
4. A Review on Different Aerobic and Anaerobic Treatment Methods in Dairy Industry Wastewater
5. Sátiro, J., Cunha, A., Gomes, A.P., Simões, R., and Albuquerque, A. (2022). Optimization of Microalgae–Bacteria Consortium in the Treatment of Paper Pulp Wastewater. Appl. Sci., 12.10.3390/app12125799 ↗
6. A Comparative Evaluation of Microalgae for the Degradation of Piggery Wastewater under Photosynthetic Oxygenation10.1016/j.biortech.2010.02.010 ↗
7. Assessing the Life-Cycle Sustainability of Algae and Bacteria-Based Wastewater Treatment Systems: High-Rate Algae Pond and Sequencing Batch Reactor10.1016/j.jenvman.2020.110459 ↗
8. CO2 Addition to Increase Biomass Production and Control Microalgae Species in High Rate Algal Ponds Treating Wastewater10.1016/j.jcou.2018.10.009 ↗
9. Hamawand, I. (2023). Energy Consumption in Water/Wastewater Treatment Industry—Optimisation Potentials. Energies, 16.10.3390/en16052433 ↗
10. Cultivation of Chlorella Vulgaris JSC-6 with Swine Wastewater for Simultaneous Nutrient/COD Removal and Carbohydrate Production10.1016/j.biortech.2015.09.067 ↗
11. Removal of Emerging Micropollutants by Activated Sludge Process and Membrane Bioreactors and the Effects of Micropollutants on Membrane Fouling: A Review10.1016/j.jece.2017.04.027 ↗
12. Rahimi, S., Modin, O., and Mijakovic, I. (2020). Technologies for Biological Removal and Recovery of Nitrogen from Wastewater. Biotechnol. Adv., 43.10.1016/j.biotechadv.2020.107570 ↗
13. Co-Cultivation of Activated Sludge and Microalgae for the Simultaneous Enhancements of Nitrogen-Rich Wastewater Bioremediation and Lipid Production10.1016/j.jtice.2018.03.038 ↗
14. Effects of Bacterial Communities on Biofuel-Producing Microalgae: Stimulation, Inhibition and Harvesting10.3109/07388551.2014.961402 ↗
15. Field Performance of Bacterial Inoculants to Alleviate Water Stress Effects in Wheat (Triticum aestivum L.)10.1007/s11104-019-04115-9 ↗
16. From Removal to Recovery: An Evaluation of Nitrogen Recovery Techniques from Wastewater10.1016/j.apenergy.2020.114616 ↗
17. Performance and Microbial Community Analysis of an Algal-Activated Sludge Symbiotic System: Effect of Activated Sludge Concentration10.1016/j.jes.2018.04.010 ↗
18. Third Generation Biofuels: A Nutritional Perspective in Enhancing Microbial Lipid Production10.1016/j.rser.2018.04.066 ↗
19. Microalgae–Bacteria Aggregates: Effect of the Hydraulic Retention Time on the Municipal Wastewater Treatment, Biomass Settleability and Methane Potential10.1002/jctb.4901 ↗
20. The Role of Starvation in Biomass Harvesting and Lipid Accumulation: Co-Culture of Microalgae–Bacteria in Synthetic Wastewater
21. Enhancement of Aerobic Granulation and Nutrient Removal by an Algal–Bacterial Consortium in a Lab-Scale Photobioreactor10.1016/j.cej.2017.11.151 ↗
22. Present and Future Opportunities in the Use of Atomic Force Microscopy to Address the Physico-Chemical Properties of Aquatic Ecosystems at the Nanoscale Level
23. Changes in Nanomechanical Properties and Adhesion Dynamics of Algal Cells during Their Growth10.1016/j.bioelechem.2019.02.011 ↗
24. Strategy for the Formation of Microalgae-Bacteria Aggregates in High-Rate Algal Ponds10.1080/09593330.2021.2014577 ↗
25. Start-up Strategies to Develop Aerobic Granular Sludge and Photogranules in Sequential Batch Reactors10.1016/j.scitotenv.2022.154402 ↗
26. Microalgae-Bacteria Consortia in High-Rate Ponds for Treating Urban Wastewater: Elucidating the Key State Indicators under Dynamic Conditions10.1016/j.jenvman.2020.110244 ↗
27. Microalgae-Based Wastewater Treatment: Mechanisms, Challenges, Recent Advances, and Future Prospects10.1016/j.ese.2022.100205 ↗
28. Show, K., and Lee, D. (2017). 8—Anaerobic Treatment Versus Aerobic Treatment, Elsevier B.V.10.1016/B978-0-444-63665-2.00008-4 ↗
29. Combining High-Rate Aerobic Wastewater Treatment with Anaerobic Digestion of Waste Activated Sludge at a Pulp and Paper Mill10.2166/wst.2018.120 ↗
30. S-RGO/ZnS Nanocomposite-Mediated Photocatalytic Pretreatment of Dairy Wastewater to Enhance Aerobic Digestion10.1007/s11814-019-0319-6 ↗
31. Lang, X., Li, Q., Xu, Y., Ji, M., Yan, G., and Guo, S. (2019). Aerobic Denitrifiers with Petroleum Metabolizing Ability Isolated from Caprolactam Sewage Treatment Pool. Bioresour. Technol., 290.10.1016/j.biortech.2019.121719 ↗
32. Effects of Different Carbon Sources and C/N Ratios on the Simultaneous Anammox and Denitrification Process10.1016/j.ibiod.2017.11.002 ↗
33. Complete Nitrification by a Single Microorganism10.1038/nature16459 ↗
34. Continuous-Flow Aerobic Granulation in Plug-Flow Bioreactors Fed with Real Domestic Wastewater10.1016/j.scitotenv.2019.06.291 ↗
35. Tolerance to Organic Loading Rate by Aerobic Granular Sludge in a Cyclic Aerobic Granular Reactor10.1016/j.biortech.2015.02.029 ↗
36. Full Scale Performance of the Aerobic Granular Sludge Process for Sewage Treatment10.1016/j.watres.2015.07.011 ↗
37. Effect of Microalgae and Bacteria Inoculation on the Startup of Bioreactors for Paper Pulp Wastewater and Biofuel Production10.1016/j.jenvman.2024.121305 ↗
38. Adsorption Mechanisms and the Effect of Oxytetracycline on Activated Sludge10.1016/j.biortech.2013.10.055 ↗
39. Energy Production from Microalgae Biomass: Carbon Footprint and Energy Balance10.1016/j.jclepro.2014.07.038 ↗
40. Assessing the Carbon Footprint of a University Campus Using a Life Cycle Assessment Approach10.1016/j.jclepro.2020.122600 ↗
41. Technical Potential of Microalgal Bacterial Floc Raceway Ponds Treating Food-Industry Effluents While Producing Microalgal Bacterial Biomass: An Outdoor Pilot-Scale Study10.1016/j.biortech.2016.07.065 ↗
42. Impacts of Long-Term Exposure to Tetracycline and Sulfamethoxazole on the Sludge Granules in an Anoxic-Aerobic Wastewater Treatment System10.1016/j.scitotenv.2019.05.313 ↗
43. Effects of Carbon Source on the Formation, Stability, Bioactivity and Biodiversity of the Aerobic Granule Sludge10.1016/j.biortech.2019.01.071 ↗
44. Wang, L., Zhan, H., Wang, Q., Wu, G., and Cui, D. (2019). Enhanced Aerobic Granulation by Inoculating Dewatered Activated Sludge under Short Settling Time in a Sequencing Batch Reactor. Bioresour. Technol., 286.10.1016/j.biortech.2019.121386 ↗
45. Synergistic Cooperation between Wastewater-Born Algae and Activated Sludge for Wastewater Treatment: Influence of Algae and Sludge Inoculation Ratios10.1016/j.biortech.2011.11.113 ↗
46. Park, C., and Dolan, S. Algal-Sludge Granule for Wastewater Treatment and Bioenergy Feedstock Generation. (10,189,732 B2), U.S. Patent.
47. Microalgal-Bacterial Consortia: From Interspecies Interactions to Biotechnological Applications10.1016/j.rser.2019.109563 ↗
48. Impact of Hydraulic Retention Time on Community Assembly and Function of Photogranules for Wastewater Treatment10.1016/j.watres.2020.115506 ↗
49. Biogranules Applied in Environmental Engineering10.1016/j.ijhydene.2017.07.176 ↗
50. Up-Scaling Aquaculture Wastewater Treatment by Microalgal Bacterial Flocs: From Lab Reactors to an Outdoor Raceway Pond10.1016/j.biortech.2014.02.113 ↗
51. Stability of Algal-Bacterial Granules in Continuous-Flow Reactors to Treat Varying Strength Domestic Wastewater10.1016/j.biortech.2017.07.134 ↗
52. Using Microbial Aggregates to Entrap Aqueous Phosphorus10.1016/j.tibtech.2020.03.012 ↗
53. Natural Sunlight Induced Rapid Formation of Water-Born Algal-Bacterial Granules in an Aerobic Bacterial Granular Photo-Sequencing Batch Reactor10.1016/j.jhazmat.2018.07.051 ↗
54. Microalgal-Bacterial Aggregates: Applications and Perspectives for Wastewater Treatment10.1016/j.biotechadv.2017.07.003 ↗
55. Development of Algae-Bacteria Granular Consortia in Photo-Sequencing Batch Reactor10.1016/j.biortech.2017.02.025 ↗
56. Ahmad, J., Zhao, Z., Zhang, Z., Shimizu, K., Utsumi, M., Lei, Z., Lee, D., and Tay, J. (2019). Algal-Bacterial Aerobic Granule Based Continuous- Flow Reactor with e Effluent Recirculation Instead of Air Bubbling: Stability and Energy Consumption Analysis. Bioresour. Technol. Rep., 7.10.1016/j.biteb.2019.100215 ↗
57. Potential of Wastewater Treatment Using a Concentrated and Suspended Algal-Bacterial Consortium in a Photo Membrane Bioreactor10.1016/j.cej.2017.10.149 ↗
58. Influence of Solar Irradiance Levels on the Formation of Microalgae-Bacteria Aggregates for Municipal Wastewater Treatment10.1016/j.algal.2017.09.011 ↗
59. Researching New Ways to Reduce N2O Emission from a Granular Sludge Sequencing Batch Reactor Treating Domestic Wastewater under Subtropical Climate Conditions10.1590/0104-6632.20190361s20170566 ↗
60. Brockmann, D., Gérand, Y., Park, C., Milferstedt, K., Hélias, A., and Hamelin, J. (2021). Wastewater Treatment Using Oxygenic Photogranule-Based Process Has Lower Environmental Impact than Conventional Activated Sludge Process. Bioresour. Technol., 319.10.1016/j.biortech.2020.124204 ↗
61. Enhanced Biomass/Biofuel Production and Nutrient Removal in an Algal Bio Fi Lm Airlift Photobioreactor10.1016/j.algal.2016.11.004 ↗
62. Algal Biofilm Reactors for Integrated Wastewater Treatment and Biofuel Production: A Review10.1016/j.cej.2015.11.062 ↗
63. Cultivation of Microalgae Chlorella sp. and Scenedesmus sp. as a Potentional Biofuel Feedstock
64. Challenges and Opportunities in Application of Microalgae (Chlorophyta) for Wastewater Treatment: A Review10.1016/j.rser.2015.07.086 ↗
65. Algal Wastewater Treatment: Photoautotrophic vs. Mixotrophic Processes10.1016/j.algal.2019.101569 ↗
66. A Review on the Use of Microalgal Consortia for Wastewater Treatment10.1016/j.algal.2016.11.008 ↗
67. Freshwater Microalgae Selection for Simultaneous Wastewater Nutrient Removal and Lipid Production10.1016/j.algal.2017.02.006 ↗
68. Characterization of a Microalga Chlorella sp. Well Adapted to Highly Concentrated Municipal Wastewater for Nutrient Removal and Biodiesel Production10.1016/j.biortech.2011.01.091 ↗
69. Algal Biorefinery: A Sustainable Approach to Valorize Algal-Based Biomass towards Multiple Product Recovery10.1016/j.biortech.2019.01.104 ↗
70. Bioflocculation: An Alternative Strategy for Harvesting of Microalgae—An Overview10.1016/j.biortech.2017.02.097 ↗
71. CO2 Bio-Mitigation Using Microalgae10.1007/s00253-008-1518-y ↗
72. Microalgal Triacylglycerols as Feedstocks for Biofuel Production: Perspectives and Advances10.1111/j.1365-313X.2008.03492.x ↗
73. Real-Time ITRAQ-Based Proteome Profiling Revealed the Central Metabolism Involved in Nitrogen Starvation Induced Lipid Accumulation in Microalgae10.1038/srep45732 ↗
74. Lipid Accumulation and Growth of Chlorella Zofingiensis in Flat Plate Photobioreactors Outdoors10.1016/j.biortech.2011.08.109 ↗
75. Chen, X., Wang, J., Wang, Q., Yuan, T., Lei, Z., Zhang, Z., Shimizu, K., and Lee, D. (2021). Simultaneous Recovery of Phosphorus and Alginate-like Exopolysaccharides from Two Types of Aerobic Granular Sludge. Bioresour. Technol., 346.10.1016/j.biortech.2021.126411 ↗
76. Algae Granulation for Nutrients Uptake and Algae Harvesting during Wastewater Treatment10.1016/j.chemosphere.2018.09.107 ↗
77. Cui, B., Chen, Z., Guo, D., and Liu, Y. (2021). Investigations on the Pyrolysis of Microalgal-Bacterial Granular Sludge: Products, Kinetics, and Potential Mechanisms. Bioresour. Technol., 349.10.1016/j.biortech.2021.126328 ↗
78. Resource Recovery from an Aerobic Granular Sludge Process Treating Domestic Wastewater10.1016/j.jwpe.2020.101148 ↗
79. Effects of Light Intensity on Oxygen Distribution, Lipid Production and Biological Community of Algal-Bacterial Granules in Photo-Sequencing Batch Reactors10.1016/j.biortech.2018.10.059 ↗
80. Effect of Solids Retention Time on the Performance of Alga-Activated Sludge Association in Municipal Wastewater Treatment and Biofuel Production10.1007/s10811-020-02076-6 ↗
81. Operation Optimization of a Photo-Sequencing Batch Reactor for Wastewater Treatment: Study on Influencing Factors and Impact on Symbiotic Microbial Ecology10.1016/j.biortech.2017.12.086 ↗
82. The Oxygenic Photogranule Process for Aeration-Free Wastewater Treatment10.1021/acs.est.8b00403 ↗
83. The Interactions of Algae-Bacteria Symbiotic System and Its Effects on Nutrients Removal from Synthetic Wastewater10.1016/j.biortech.2017.09.074 ↗
84. Nitrogen Removal and Microbial Community for the Treatment of Rural Domestic Sewage with Low C/N Ratio by A/O Biofilter with Arundo Donax as Carbon Source and Filter Media10.1016/j.jwpe.2020.101509 ↗
85. Effect of TiO2nanoparticles on Aerobic Granulation of Algal-Bacterial Symbiosis System and Nutrients Removal from Synthetic Wastewater10.1016/j.biortech.2015.03.118 ↗
86. Effect of Algae Growth on Aerobic Granulation and Nutrients Removal from Synthetic Wastewater by Using Sequencing Batch Reactors10.1016/j.biortech.2014.12.024 ↗
87. Characteristics and Performance of Aerobic Algae-Bacteria Granular Consortia in a Photo-Sequencing Batch Reactor10.1016/j.jhazmat.2018.01.059 ↗
88. Mhedhbi, E., Khelifi, N., Foladori, P., and Smaali, I. (2020). Real-Time Behavior of a Microalgae-Bacteria Consortium Treating Wastewater in a Sequencing Batch Reactor in Response to Feeding Time and Agitation Mode. Water, 12.10.3390/w12071893 ↗
89. Activity Determination of an Algal-Bacterial Consortium Developed during Wastewater Treatment Based on Oxygen Evolution10.1016/j.jwpe.2020.101278 ↗
90. Development of a Photo-Baffled Reactor for Microalgae-Nitrifying Bacteria Consortia: Achieving Long-Term, Stable Partial Nitrification10.1016/j.jece.2021.106082 ↗
91. Convergent Community Structure of Algal—Bacterial Consortia and Its Effects on Advanced Wastewater Treatment and Biomass Production10.1038/s41598-021-00517-x ↗
92. Kang, D., and Kim, K. (2021). Real Wastewater Treatment Using a Moving Bed and Wastewater-Borne Algal–Bacterial Consortia with a Short Hydraulic Retention Time. Processes, 9.10.3390/pr9010116 ↗
93. Influence of Wavelength Photoperiods and N/P Ratio on Wastewater Treatment with Microalgae–Bacteria10.2166/wst.2021.257 ↗
94. Wang, J., Wei, Y., Li, Z., Chen, X., Lei, Z., Yuan, T., Shimizu, K., Zhang, Z., Kim, S., and Lee, D. (2022). Effect of Stepwise or One-Time Illumination Strategy on the Development of Algal-Bacterial Aerobic Granular Sludge in Sequencing Batch Reactor. Bioresour. Technol. Rep., 17.10.1016/j.biteb.2021.100931 ↗
95. Urban Wastewater Treatment by Microalgae, Bacteria and Microalgae–Bacteria System (Laboratory-Scale Study)10.1080/1573062X.2021.1974892 ↗
96. Characteristics of Algal-Bacterial Aerobic Granular Sludge Treating Real Wastewater: Effects of Algal Inoculation and Alginate-like Exopolymers Recovery10.1016/j.chemosphere.2023.138595 ↗
97. Disintegration and Re-Granulation of Aged Algal-Bacterial Aerobic Granular Sludge in Wastewater Treatment10.1016/j.jwpe.2023.104614 ↗
98. Perspectives on the Feasibility of Using Microalgae for Industrial Wastewater Treatment10.1016/j.biortech.2016.09.106 ↗
99. Treatment of Real Wastewater Using Co-Culture of Immobilized Chlorella Vulgaris and Suspended Activated Sludge10.1016/j.watres.2017.04.078 ↗
100. A Novel Membrane Bioreactor Inoculated with Symbiotic Sludge Bacteria and Algae: Performance and Microbial Community Analysis10.1016/j.biortech.2017.12.048 ↗