Tissue Engineering 3D Porous Scaffolds Prepared from Electrospun Recombinant Human Collagen (RHC) Polypeptides/Chitosan Nanofibers
Source abstract
Abstract
Electrospinning, the only method that can continuously produce nanofibers, has been widely used to prepare nanofibers for tissue engineering applications. However, electrospinning is not suitable for preparing clinically relevant three-dimensional (3D) nanofibrous scaffolds with hierarchical pore structures. In this study, recombinant human collagen (RHC)/chitosan nanofibers prepared by electrospinning were combined with porous scaffolds produced by freeze drying to fabricate 3D nanofibrous scaffolds. These scaffolds exhibited high porosity (over 80%) and an interconnected porous structure (ranging from sub-micrometers to 200 μm) covered with nanofibers. As confirmed by the characterization results, these scaffolds showed good swelling ability, stability, and adequate mechanical strength, making it possible to use the 3D nanofibrous scaffolds in various tissue engineering applications. In addition, after seven days of cell culturing, NIH 3T3 was infiltrated into the scaffolds while maintaining its morphology and with superior proliferation and viability. These results indicated that the 3D nanofibrous scaffolds hold great promise for tissue engineering applications.
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Research context
OpenAlex topics: Electrospun Nanofibers in Biomedical Applications · Bone Tissue Engineering Materials · Tissue Engineering and Regenerative Medicine
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China
Biomanufacturing Center, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China
Funding
National High Technology Research and Development Program of China
References (44)
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