Optimization of Microalgae–Bacteria Consortium in the Treatment of Paper Pulp Wastewater
Source abstract
Abstract
The microalgae–bacteria consortium is a promising and sustainable alternative for industrial wastewater treatment, since it may allow good removal of organic matter and nutrients, as well as the possibility of producing products with added value from the algae biomass. This research investigated the best bacterial and microalgae inoculation ratio for system start-up and evaluation of removing organic matter (as chemical oxygen demand (COD)), ammoniacal nitrogen (NH4+–N), nitrite nitrogen (NO2−–N), nitrate nitrogen (NO3−–N), phosphate phosphorus (PO43−–P) and biomass formation parameters in six photobioreactors with a total volume of 1000 mL. Reactors were operated for 14 days with the following ratios of pulp mill biomass aerobic (BA) and Scenedesmus sp. microalgae (MA): 0:1 (PBR1), 1:0 (PBR2), 1:1 (PBR3), 3:1 (PBR4), 5:1 (PBR5), and 1:3 (PBR6). Results show that COD removal was observed in just two days of operation in PBR4, PBR5, and PBR6, whereas for the other reactors (with a lower rate of initial inoculation) it took five days. The PBR5 and PBR6 performed better in terms of NH4+–N removal, with 86.81% and 77.11%, respectively, which can be attributed to assimilation by microalgae and nitrification by bacteria. PBR6, with the highest concentration of microalgae, had the higher PO43−–P removal (86%), showing the advantage of algae in consortium with bacteria for phosphorus uptake. PBR4 and PBR5, with the highest BA, led to a better biomass production and sedimentability on the second day of operation, with flocculation efficiencies values over 90%. Regarding the formation of extracellular polymeric substances (EPS), protein production was substantially higher in PBR4 and PBR5, with more BA, with average concentrations of 49.90 mg/L and 49.05 mg/L, respectively. The presence of cyanobacteria and Chlorophyceae was identified in all reactors except PBR1 (only MA), which may indicate a good formation and structuring of the microalgae–bacteria consortium. Scanning electron microscopy (SEM) analysis revealed that filamentous microalgae were employed as a foundation for the fixation of bacteria and other algae colonies.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Algal biology and biofuel production · Wastewater Treatment and Nitrogen Removal · Microbial Fuel Cells and Bioremediation
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
FibEnTech, GeoBioTec, Department of Civil Engineering and Architecture, University of Beira Interior, 6201-001 Covilhã, Portugal
FibEnTech, Departament of Chemistry, University of Beira Interior, 6201-001 Covilhã, Portugal
FibEnTech and Optical Center, University of Beira Interior, 6201-001 Covilhã, Portugal
Funding
Santander Totta Bank
References (68)
Show source-provided references
1. A review on anaerobic-aerobic treatment of industrial and municipal wastewater10.1016/j.cej.2009.06.041 ↗
2. Life cycle assessment of wastewater treatment in developing countries: A review10.1016/j.watres.2019.01.010 ↗
3. A review on different aerobic and anaerobic treatment methods in dairy industry wastewater
4. Eilertsen, H.C., Eriksen, G.K., Bergum, J.-S., Strømholt, J., Elvevoll, E., Eilertsen, K.-E., Heimstad, E.S., Giæver, I.H., Israelsen, L., and Svenning, J.B. (2022). Mass Cultivation of Microalgae: I. Experiences with Vertical Column Airlift Photobioreactors, Diatoms and CO2 Sequestration. Appl. Sci., 12.10.3390/app12063082 ↗
5. A comparative evaluation of microalgae for the degradation of piggery wastewater under photosynthetic oxygenation10.1016/j.biortech.2010.02.010 ↗
6. Bioflocculation: An alternative strategy for harvesting of microalgae—An overview10.1016/j.biortech.2017.02.097 ↗
7. Integrating nutrient removal and solid management restricts the feasibility of algal biofuel generation via wastewater treatment10.1016/j.algal.2016.11.019 ↗
8. Algal biorefinery: A sustainable approach to valorize algal-based biomass towards multiple product recovery10.1016/j.biortech.2019.01.104 ↗
9. Viswanaathan, S., Perumal, P.K., and Sundaram, S. (2022). Integrated Approach for Carbon Sequestration and Wastewater Treatment Using Algal-Bacterial Consortia: Opportunities and Challenges. Sustainability, 14.10.3390/su14031075 ↗
10. Improved microalgal productivity and nutrient removal through operating wastewater high rate algal ponds in series10.1016/j.algal.2020.101850 ↗
11. Bioresource Technology Treatment of industrial wastewaters by microalgal bacterial flocs in sequencing batch reactors10.1016/j.biortech.2014.03.057 ↗
12. Aerobic denitrifiers with petroleum metabolizing ability isolated from caprolactam sewage treatment pool10.1016/j.biortech.2019.121719 ↗
13. Show, K.-Y., and Lee, D.-J. (2017). Anaerobic Treatment. Current Developments in Biotechnology and Bioengineering. Biological Treatment of Industrial Effluents, Elsevier. Chapter 8.
14. Microalgal biomass and lipid production in mixed municipal, dairy, pulp and paper wastewater together with added flue gases10.1016/j.biortech.2014.06.061 ↗
15. Porto, B., Gonçalves, A.L., Esteves, A.F., De Souza, S.M.A.G.U., De Souza, A.A.U., Vilar, V.J.P., and Pires, J.C.M. (2020). Microalgal growth in paper industry effluent: Coupling biomass production with nutrients removal. Appl. Sci., 10.10.3390/app10093009 ↗
16. Silva, M., Gonçalves, A., Vilar, V., and Pires, J. (2021). Experimental and techno-economic study on the use of microalgae for paper industry effluents remediation. Sustainability, 13.10.3390/su13031314 ↗
17. Removal of nutrients and organic pollution load from pulp and paper mill effluent by microalgae in outdoor open pond10.1016/j.biortech.2016.04.060 ↗
18. Linking microbial diversity and population dynamics to the removal efficiency of pharmaceutically active compounds (PhACs) in an anaerobic/anoxic/aerobic (A2O) system10.1016/j.chemosphere.2019.06.017 ↗
19. Biodegradation of carbamazepine using freshwater microalgae Chlamydomonas mexicana and Scenedesmus obliquus and the determination of its metabolic fate10.1016/j.biortech.2016.01.038 ↗
20. Screening of two freshwater green microalgae in pulp and paper mill wastewater effluents in Nova Scotia, Canada10.2166/wst.2021.001 ↗
21. Regulatory function of organic carbon supplementation on biodiesel production during growth and nutrient stress phases of mixotrophic microalgae cultivation10.1016/j.biortech.2014.02.102 ↗
22. Microalgae lipid and biomass for biofuel production: A comprehensive review on lipid enhancement strategies and their effects on fatty acid composition10.1016/j.rser.2018.07.050 ↗
23. Heterotrophic microalgae cultivation to synergize biodiesel production with waste remediation: Progress and perspectives10.1016/j.biortech.2014.10.056 ↗
24. Wastewater treatment in the pulp-and-paper industry: A review of treatment processes and the associated greenhouse gas emission10.1016/j.jenvman.2015.05.010 ↗
25. Cost-effective removal of COD in the pre-treatment of wastewater from the paper industry10.2166/wst.2019.328 ↗
26. Co-cultivation of activated sludge and microalgae for the simultaneous enhancements of nitrogen-rich wastewater bioremediation and lipid production10.1016/j.jtice.2018.03.038 ↗
27. Synergistic cooperation between wastewater-born algae and activated sludge for wastewater treatment: Influence of algae and sludge inoculation ratios10.1016/j.biortech.2011.11.113 ↗
28. APHA, AWWA, and WPCF (2017). Standard Methods for the Examination of Water and Wastewater, Water Environment Federation. [23rd ed.].
29. New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural phytoplankton10.1016/S0015-3796(17)30778-3 ↗
30. A revised method for pigment extraction from marine nannoplanktonic algal cultures
31. Influence of solar irradiance levels on the formation of microalgae-bacteria aggregates for municipal wastewater treatment10.1016/j.algal.2017.09.011 ↗
32. Colorimetric Method for Determination of Sugars and Related Substances10.1021/ac60111a017 ↗
33. Protein measurement with the folin phenol reagent10.1016/S0021-9258(19)52451-6 ↗
34. A Rapid Method Of Total Lipid Extraction And Purification
35. Methanol fixation of plant tissue for Scanning Electron Microscopy improves preservation of tissue morphology and dimensions10.1186/1746-4811-9-36 ↗
36. Temperature effect on microalgae: A crucial factor for outdoor production10.1007/s11157-013-9310-6 ↗
37. Long term diurnal variations in contaminant removal in high rate ponds treating urban wastewater10.1016/j.biortech.2005.07.019 ↗
38. Autoflocculation of microalgae, via magnesium hydroxide precipitation, in a high rate algal pond treating municipal wastewater in the South Australian Riverland10.1016/j.algal.2021.102418 ↗
39. Renewable and sustainable bioenergies production from palm oil mill effluent (POME): Win-win strategies toward better environmental protection10.1016/j.biotechadv.2010.10.001 ↗
40. CO2 addition to increase biomass production and control microalgae species in high rate algal ponds treating wastewater10.1016/j.jcou.2018.10.009 ↗
41. Effect of aeration rate on performance and stability of algal-bacterial symbiosis system to treat domestic wastewater in sequencing batch reactors10.1016/j.biortech.2016.09.123 ↗
42. Flow cytometry for rapid characterisation of microbial community dynamics in waste stabilisation ponds10.1016/j.watres.2019.115243 ↗
43. Nitrous oxide emission during wastewater treatment10.1016/j.watres.2009.03.001 ↗
44. Technologies for biological removal and recovery of nitrogen from wastewater10.1016/j.biotechadv.2020.107570 ↗
45. Algae granulation for nutrients uptake and algae harvesting during wastewater treatment10.1016/j.chemosphere.2018.09.107 ↗
46. Microalgal-bacterial aggregates for wastewater treatment: A mini-review
47. Influence of bed media characteristics on ammonia and nitrate removal in shallow horizontal subsurface flow constructed wetlands10.1016/j.biortech.2009.07.016 ↗
48. Zhang, Q., Xu, X., Zhou, X., and Chen, C. (2022). Recent Advances in Autotrophic Biological Nitrogen Removal. Water, 14.10.3390/w14071101 ↗
49. Enhanced aerobic granulation by inoculating dewatered activated sludge under short settling time in a sequencing batch reactor10.1016/j.biortech.2019.121386 ↗
50. Advances in enhanced biological phosphorus removal: From micro to macro scale10.1016/j.watres.2007.02.030 ↗
51. Stability of algal-bacterial granules in continuous-flow reactors to treat varying strength domestic wastewater10.1016/j.biortech.2017.07.134 ↗
52. Characteristics and performance of aerobic algae-bacteria granular consortia in a photo-sequencing batch reactor10.1016/j.jhazmat.2018.01.059 ↗
53. Lin, C., Cao, P., Xu, X., and Ye, B. (2019). Algal-bacterial symbiosis system treating high-load printing and dyeing wastewater in continuous-flow reactors under natural light. Water, 11.10.3390/w11030469 ↗
54. Comparison of Scenedesmus acuminatus and Chlorella vulgaris cultivation in liquid digestates from anaerobic digestion of pulp and paper industry and municipal wastewater treatment sludge10.1007/s10811-017-1175-6 ↗
55. Response of algal-bacterial granular system to low carbon wastewater: Focus on granular stability, nutrients removal and accumulation10.1016/j.biortech.2018.07.114 ↗
56. Development of algae-bacteria granular consortia in photo-sequencing batch reactor10.1016/j.biortech.2017.02.025 ↗
57. Researching new ways to reduce N2O emission from a granular sludge sequencing batch reactor treating domestic wastewater under subtropical climate conditions10.1590/0104-6632.20190361s20170566 ↗
58. Using Microbial Aggregates to Entrap Aqueous Phosphorus10.1016/j.tibtech.2020.03.012 ↗
59. Biogranules applied in environmental engineering10.1016/j.ijhydene.2017.07.176 ↗
60. Quantification of chlorophyll a, chlorophyll b and pheopigments a in lake sediments through deconvolution of bulk UV–VIS absorption spectra10.1007/s10933-020-00135-z ↗
61. Microalgae-bacteria aggregates: Effect of the hydraulic retention time on the municipal wastewater treatment, biomass settleability and methane potential10.1002/jctb.4901 ↗
62. Microalgal-bacterial aggregates: Applications and perspectives for wastewater treatment10.1016/j.biotechadv.2017.07.003 ↗
63. Activity determination of an algal-bacterial consortium developed during wastewater treatment based on oxygen evolution10.1016/j.jwpe.2020.101278 ↗
64. Potential yields and properties of oil from the hydrothermal liquefaction of microalgae with different biochemical content10.1016/j.biortech.2010.06.028 ↗
65. A general kinetic model for the hydrothermal liquefaction of microalgae10.1016/j.biortech.2014.04.013 ↗
66. Salian, K., and Strezov, V. (2017). Biofuels From Microalgae. Encyclopedia of Sustainable Technologies, Elsevier. Volume 3.10.1016/B978-0-12-409548-9.10114-9 ↗
67. Cultivation, photobioreactor design and harvesting of microalgae for biodiesel production: A critical review10.1016/j.biortech.2010.06.159 ↗
68. Valchev, D., and Ribarova, I. (2022). A Review on the Reliability and the Readiness Level of Microalgae-Based Nutrient Recovery Technologies for Secondary Treated Effluent in Municipal Wastewater Treatment Plants. Processes, 10.10.3390/pr10020399 ↗