Encapsulated Probiotics: Potential Techniques and Coating Materials for Non-Dairy Food Applications
Source abstract
Abstract
The growing health awareness among consumers has increased the demand for non-dairy-based products containing probiotics. However, the incorporation of probiotics in non-dairy matrices is challenging, and probiotics tend to have a low survival rate in these matrices and subsequently perform poorly in the gastrointestinal system. Encapsulation of probiotics with a physical barrier could preserve the survivability of probiotics and subsequently improve delivery efficiency to the host. This article aimed to review the effectiveness of encapsulation techniques (coacervation, extrusion, emulsion, spray-drying, freeze-drying, fluidized bed coating, spray chilling, layer-by-layer, and co-encapsulation) and biomaterials (carbohydrate-, fat-, and protein-based) on the viability of probiotics under the harsh conditions of food processing, storage, and along the gastrointestinal passage. Recent studies on probiotic encapsulations using non-dairy food matrices, such as fruits, fruit and vegetable juices, fermented rice beverages, tea, jelly-like desserts, bakery products, sauces, and gum products, were also included in this review. Overall, co-encapsulation of probiotics with prebiotics was found to be effective in preserving the viability of probiotics in non-dairy food matrices. Encapsulation techniques could add value and widen the application of probiotics in the non-dairy food market and future perspectives in this area.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Probiotics and Fermented Foods · Microencapsulation and Drying Processes · Food composition and properties
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Faculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, Sabah, Malaysia
Food Technology Division, School of Industrial Technology, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia
Renewable Biomass Transformation Cluster, School of Industrial Technology, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia
Australasian Nanoscience and Nanotechnology Initiative, Monash University, Melbourne, VIC 3168, Australia
Funding
Universiti Malaysia Sabah, SPLB scheme
References (98)
Show source-provided references
1. Probiotics, prebiotics, and microencapsulation: A review10.1080/10408398.2014.887055 ↗
2. Non-dairy probiotic food products: An emerging group of functional foods10.1080/10408398.2018.1462760 ↗
3. FAO/WHO (2002). FAO/WHO Joint Working Group Report on Drafting Guidelines for the Evaluation of Probiotics in Food (30 April 2002 and 1 May 2002), Scientific Research Publishing.
4. Sanders, M.E., Goh, Y.J., and Klaenhammer, T.R. (2019). Probiotics and Prebiotics. Food Microbiology: Fundamentals and Frontiers, ASM Press.10.1128/9781555819972.ch32 ↗
5. Protective effect of a cross-linked starch by extrusion on the survival of Bifidobacterium breve ATCC 15700 in yogurt
6. Encapsulation, storage viability, and consumer acceptance of probiotic butter10.1016/j.lwt.2020.110536 ↗
7. Survival and stability of free and encapsulated probiotic bacteria under simulated gastrointestinal conditions and in pasteurized grape juice10.1111/jfpp.14346 ↗
8. Optimization of microencapsulated Lactobacillus rhamnosus GG from whey protein and glutinous rice starch by spray drying10.4028/www.scientific.net/KEM.859.265 ↗
9. Aspri, M., Papademas, P., and Tsaltas, D. (2020). Review on non-dairy probiotics and their use in non-dairy based products. Fermentation, 6.10.3390/fermentation6010030 ↗
10. Probiotic survival and in vitro digestion of L. salivarius spp. salivarius encapsulated by high homogenization pressures and incorporated into a fruit matrix10.1016/j.lwt.2019.05.088 ↗
11. Probiotic dried apple snacks: Development of probiotic coating and shelf-life studies
12. Bilayer edible coating with stabilized Lactobacillus plantarum 299v improved the shelf life and safety quality of fresh-cut apple slices10.1016/j.fpsl.2021.100746 ↗
13. Effect of encapsulation on the stability and survivability of Enterococcus faecium in a non-dairy probiotic beverage10.1177/1082013218813823 ↗
14. Sugarcane juice with co-encapsulated Bifidobacterium animalis subsp. lactis BLC1 and proanthocyanidin-rich cinnamon extract10.1007/s12602-019-09605-x ↗
15. Survival of encapsulated probiotics in pasteurized grape juice and evaluation of their properties during storage10.1177/1082013218801113 ↗
16. Probiotication of tomato and carrot juices for shelf-life enhancement using micro-encapsulation
17. Alginate-persian gum-prebiotics microencapsulation impacts on the survival rate of Lactococcus lactis ABRIINW-N19 in orange juice10.1016/j.lwt.2020.109190 ↗
18. Survival of microencapsulated Lactobacillus casei (prepared by vibration technology) in fruit juice during cold storage10.1016/j.ejbt.2019.10.002 ↗
19. Santos Monteiro, S., Albertina Silva Beserra, Y., Miguel Lisboa Oliveira, H., and Pasquali, M.A.d.B. (2020). Production of probiotic passion fruit (Passiflora edulis Sims f. Flavicarpa Deg.) drink using Lactobacillus reuteri and microencapsulation via spray drying. Foods, 9.10.3390/foods9030335 ↗
20. Characterization of spray dried probiotic Sohiong fruit powder with Lactobacillus plantarum10.1016/j.lwt.2019.108699 ↗
21. Survival and behavior of encapsulated probiotics (Lactobacillus plantarum) in calcium-alginate-soy protein isolate-based hydrogel beads in different processing conditions (pH and temperature) and in pasteurized mango juice10.1155/2019/9768152 ↗
22. Viability of microencapsulated Lactobacillus acidophilus by complex coacervation associated with enzymatic crosslinking under application in different fruit juices10.1016/j.foodres.2021.110190 ↗
23. Freeze dried probiotic carrot juice powder for better storage stability of probiotic
24. From by-product to functional food: The survival of L. casei shirota, L. casei immunitas and L. acidophilus johnsonii, during spray drying in orange juice using a maltodextrin/pectin mixture as carrier10.1080/14786419.2022.2032049 ↗
25. Improved viability of microencapsulated probiotics in a freeze-dried banana powder during storage and under simulated gastrointestinal tract10.1007/s12602-018-9464-1 ↗
26. Nilubol, S., and Wanchaitanawong, P. (2017, January 10). Viability of Lactobacillus plantarum TISTR 2075 in carrot tablet after fluidized bed drying. Proceedings of the The National and International Graduate Research Conference, Khon Kaen, Thailand.
27. Co-encapsulation of Dictyophora indusiata to improve Lactobacillus acidophilus survival and its effect on quality of sweet fermented rice (Khoa-Mak) sap beverage10.1007/s13197-021-05101-9 ↗
28. Encapsulation of probiotics in whey protein isolate and modified huauzontle’s starch: An approach to avoid fermentation and stabilize polyphenol compounds in a ready-to-drink probiotic green tea10.1016/j.lwt.2020.109131 ↗
29. Microencapsulation of Lactobacillus acidophilus NCFM incorporated with mannitol and its storage stability in mulberry tea
30. Probiotication of black grass jelly [Mesona chinensis (Benth.)] by encapsulated Lactobacillus plantarum Mar8 for a ready to drink (RTD) beverages
31. The use of probiotic-loaded single-and double-layered microcapsules in cake production10.1007/s12602-018-9467-y ↗
32. Fluidized bed microencapsulation of Lactobacillus sporogenes with some selected hydrocolloids for probiotic bread production
33. Survival survey of Lactobacillus acidophilus in additional probiotic bread
34. Spray chilling microencapsulation of Lactobacillus acidophilus and Bifidobacterium animalis subsp. lactis and its use in the preparation of savory probiotic cereal bars10.1007/s11947-016-1724-z ↗
35. Evaluation of survival rate and physicochemical properties of encapsulated bacteria in alginate and resistant starch in mayonnaise sauce
36. Effect of encapsulation and wall material on the probiotic survival and physicochemical properties of synbiotic chewing gum: Study with univariate and multivariate analyses10.1016/j.heliyon.2019.e02144 ↗
37. Encapsulation of Lactobacillus casei in quince seed gum-alginate beads to produce a functional synbiotic drink powder by agro-industrial by-products and freeze-drying10.1016/j.foodhyd.2021.106895 ↗
38. Trends in dairy and non-dairy probiotic products-a review10.1007/s13197-015-1795-2 ↗
39. Maciel, M.I.S., and de Souza, M.M.B. (2020). Prebiotics and Probiotics-Potential Benefits in Human Nutrition and Health. Prebiotics and Probiotics-Potential Benefits in Nutrition and Health, IntechOpen.10.5772/intechopen.89155 ↗
40. Microencapsulation: An overview for the survival of probiotic bacteria
41. Pech-Canul, A.d.l.C., Ortega, D., García-Triana, A., González-Silva, N., and Solis-Oviedo, R.L. (2020). A brief review of edible coating materials for the microencapsulation of probiotics. Coatings, 10.10.3390/coatings10030197 ↗
42. Progress in microencapsulation of probiotics: A review10.1111/1541-4337.12532 ↗
43. A review of the microencapsulation techniques for the incorporation of probiotic bacteria in functional foods10.1080/10408398.2020.1761773 ↗
44. Encapsulated probiotic cells: Relevant techniques, natural sources as encapsulating materials and food applications-a narrative review10.1016/j.foodres.2020.109682 ↗
45. A review on microencapsulation in improving probiotic stability for beverages application10.24191/sl.v14i1.7900 ↗
46. Comparison of extrusion and co-extrusion encapsulation techniques to protect Lactobacillus acidophilus LA3 in simulated gastrointestinal fluids10.1016/j.lwt.2017.11.008 ↗
47. Encapsulation of probiotic Lactobacillus acidophilus by ionic gelation with electrostatic extrusion for enhancement of survival under simulated gastric conditions and during refrigerated storage10.1111/ijfs.13308 ↗
48. On the encapsulation and viability of probiotic bacteria in edible carboxymethyl cellulose-gelatin water-in-water emulsions10.1016/j.foodhyd.2017.09.014 ↗
49. Development of a probiotic delivery system based on gelation of water-in-oil emulsions10.1016/j.lwt.2017.07.045 ↗
50. Soy protein incorporated nanoemulsion for enhanced stability of probiotic (Lactobacillus delbrueckii subsp. bulgaricus) and its characterization
51. Lactobacillus acidophilus loaded pickering double emulsion with enhanced viability and colon-adhesion efficiency10.1016/j.lwt.2019.108928 ↗
52. Enhancing the storage and gastrointestinal passage viability of probiotic powder (Lactobacillus Plantarum) through encapsulation with pickering high internal phase emulsions stabilized with WPI-EGCG covalent conjugate nanoparticles10.1016/j.foodhyd.2021.106658 ↗
53. Microencapsulation in food chemistry
54. Use of whey protein isolate and gum Arabic for the co-encapsulation of probiotic Lactobacillus plantarum and phytosterols by complex coacervation: Enhanced viability of probiotic in Iranian white cheese10.1016/j.foodhyd.2020.106496 ↗
55. Complex coacervation: Principles, mechanisms and applications in microencapsulation10.1016/j.ijbiomac.2018.10.144 ↗
56. Probiotic encapsulation in water-in-water emulsion via heteroprotein complex coacervation of type-A gelatin/sodium caseinate10.1016/j.foodhyd.2020.105790 ↗
57. Microencapsulation of probiotic Saccharomyces cerevisiae var. boulardii with different wall materials by spray drying10.1016/j.lwt.2015.03.034 ↗
58. Direct spray drying and microencapsulation of probiotic Lactobacillus reuteri from slurry fermentation with whey10.1111/jam.12293 ↗
59. Use of prebiotic sources to increase probiotic viability in pectin microparticles obtained by emulsification/internal gelation followed by freeze-drying10.1016/j.foodres.2019.108902 ↗
60. Viability of L. acidophilus microcapsules and their application to buffalo milk yoghurt10.1016/j.fbp.2012.08.009 ↗
61. Effect of encapsulant and cryoprotectant on the viability of probiotic Pediococcus acidilactici ATCC 8042 during freeze-drying and exposure to high acidity, bile salts and heat10.1016/j.lwt.2017.04.009 ↗
62. Protective approaches and mechanisms of microencapsulation to the survival of probiotic bacteria during processing, storage and gastrointestinal digestion: A review10.1080/10408398.2017.1377684 ↗
63. Single and double layered microencapsulation of probiotics by spray drying and spray chilling10.1016/j.lwt.2017.03.060 ↗
64. Encapsulation of probiotics: Insights into academic and industrial approaches10.3934/matersci.2016.1.114 ↗
65. Incorporation of Bifidobacterium sp. into powder products through a fluidized bed process for enteric targeted release10.3168/jds.2020-18516 ↗
66. Drying techniques of probiotic bacteria as an important step towards the development of novel pharmabiotics10.1016/j.ijpharm.2016.04.002 ↗
67. Călinoiu, L.-F., Ştefănescu, B.E., Pop, I.D., Muntean, L., and Vodnar, D.C. (2019). Chitosan coating applications in probiotic microencapsulation. Coatings, 9.10.3390/coatings9030194 ↗
68. Influence of multilayer microencapsulation on the viability of Lactobacillus casei using a combined double emulsion and ionic gelation approach10.1016/j.fbp.2020.08.009 ↗
69. Physiological protection of probiotic microcapsules by coatings10.1080/10408398.2017.1289148 ↗
70. Co-encapsulation of bioactive compounds from blackberry juice and probiotic bacteria in biopolymeric matrices10.1016/j.lwt.2019.04.064 ↗
71. Samedi, L., and Charles, A.L. (2019). Viability of 4 probiotic bacteria microencapsulated with arrowroot starch in the simulated gastrointestinal tract (GIT) and yoghurt. Foods, 8.10.3390/foods8050175 ↗
72. Double layer co-encapsulation of probiotics and prebiotics by electro-hydrodynamic atomization10.1016/j.lwt.2019.04.040 ↗
73. Co-extrusion encapsulation of probiotic Lactobacillus acidophilus alone or together with apple skin polyphenols: An aqueous and value-added delivery system using alginate10.1007/s11947-013-1129-1 ↗
74. Gheorghita Puscaselu, R., Lobiuc, A., Dimian, M., and Covasa, M. (2020). Alginate: From food industry to biomedical applications and management of metabolic disorders. Polymers, 12.10.3390/polym12102417 ↗
75. Martău, G.A., Mihai, M., and Vodnar, D.C. (2019). The use of chitosan, alginate, and pectin in the biomedical and food sector-biocompatibility, bioadhesiveness, and biodegradability. Polymers, 11.10.3390/polym11111837 ↗
76. Pavli, F., Tassou, C., Nychas, G.-J.E., and Chorianopoulos, N. (2018). Probiotic incorporation in edible films and coatings: Bioactive solution for functional foods. Int. J. Mol. Sci., 19.10.3390/ijms19010150 ↗
77. Perspective on constructing cellulose-hydrogel-based gut-like bioreactors for growth and delivery of multiple-strain probiotic bacteria10.1021/acs.jafc.1c00468 ↗
78. Effect of inlet air temperature and gum Arabic concentration on encapsulation of probiotics by spray drying10.1016/j.lwt.2018.10.022 ↗
79. Kwiecień, I., and Kwiecień, M. (2018). Application of polysaccharide-based hydrogels as probiotic delivery systems. Gels, 4.10.3390/gels4020047 ↗
80. Lara-Espinoza, C., Carvajal-Millán, E., Balandrán-Quintana, R., López-Franco, Y., and Rascón-Chu, A. (2018). Pectin and pectin-based composite materials: Beyond food texture. Molecules, 23.10.3390/molecules23040942 ↗
81. Pectins functionalized biomaterials; a new viable approach for biomedical applications: A review10.1016/j.ijbiomac.2017.03.029 ↗
82. Biomaterial-based encapsulated probiotics for biomedical applications: Current status and future perspectives10.1016/j.matdes.2021.110018 ↗
83. Xanthan gum derivatives: Review of synthesis, properties and diverse applications10.1039/D0RA04366D ↗
84. Microencapsulating polymers for probiotics delivery systems: Preparation, characterization, and applications10.1016/j.foodhyd.2021.106882 ↗
85. Gelatin from camel skins: Extraction and characterizations10.1016/j.foodhyd.2019.105457 ↗
86. Functionality of dairy proteins and vegetable proteins in nutritional supplement powders: A review
87. Whey proteins and its derivatives: Bioactivity, functionality, and current applications10.3390/dairy1030016 ↗
88. Preparation and properties of milk proteins-based encapsulated probiotics: A review10.1007/s13594-015-0223-8 ↗
89. Protein-based delivery systems for the nanoencapsulation of food ingredients10.1111/1541-4337.12360 ↗
90. Alginate-based and protein-based materials for probiotics encapsulation: A review10.1111/ijfs.12078 ↗
91. Nahum, V., and Domb, A.J. (2021). Recent developments in solid lipid microparticles for food ingredients delivery. Foods, 10.10.3390/foods10020400 ↗
92. Probiotic encapsulation10.5897/AJMR2013.5718 ↗
93. Dordevic, S., Dordevic, D., Sedlacek, P., Kalina, M., Tesikova, K., Antonic, B., Tremlova, B., Treml, J., Nejezchlebova, M., and Vapenka, L. (2021). Incorporation of Natural Blueberry, Red Grapes and Parsley Extract By-Products into the Production of Chitosan Edible Films. Polymers, 13.10.3390/polym13193388 ↗
94. The efficiency of polysaccharide microencapsulation in improving survival of probiotic bacteria
95. Encapsulation of Lactobacillus salivarius in single and dual biopolymer10.1016/j.jfoodeng.2020.110398 ↗
96. In vitro survival of Bifidobacterium bifidum microencapsulated in zein-coated alginate hydrogel microbeads10.1080/02652048.2019.1618403 ↗
97. Effect of catechins on the growth of oxygen-sensitive probiotic bacteria10.1016/j.foodres.2012.10.014 ↗
98. Peris, M., Rubio-Arraez, S., Castelló, M.L., and Ortolá, M.D. (2019). From the laboratory to the kitchen: New alternatives to healthier bakery products. Foods, 8.10.3390/foods8120660 ↗