Antibacterial and Antifungal Activities of Proteins Extracted from Seven Different Snails
Source abstract
Abstract
Snails have been used both as a food and as a treatment for a variety of medicinal conditions. In this study, seven different snail proteins were evaluated for their antimicrobial activity. Fresh water and land snails of seven different live species were collected and identified. Crude proteins were extracted from seven different snails. The extracted proteins were estimated using Bradford’s method and snail proteins were displayed using a sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The seven different snail proteins were evaluated for their antimicrobial activity against various pathogenic bacterial and fungal cultures by agar well diffusion method and MIC (Minimum Inhibitory Concentration). One of the most active, crude proteins was from land snail Cryptozona bistrialis and its protein was capable of completely inhibiting the development of pathogenic bacterial and fungal cultures. This study shows that the land snail C. bistrialis proteins could be used as an antibiotic in biomedical research.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Insect Utilization and Effects · Protein Hydrolysis and Bioactive Peptides · Aquaculture Nutrition and Growth
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Institute of Marine Biotechnology, Pukyong National University, Busan 48513, Korea
Department of Chemistry, Pukyong National University, Busan 48513, Korea
Funding
Korea Institute of Marine Science & Technology Promotion
References (24)
Show source-provided references
1. Purification and partial characterization of human neutrophil elastase inhibitors from the marine snail Cenchritis muricatus (Mollusca)10.1016/j.cbpa.2006.01.022 ↗
2. Antibiotic-Resistant Bugs in the 21st Century—A Clinical Super-Challenge10.1056/NEJMp0804651 ↗
3. Marine natural products10.1039/b805113p ↗
4. Antimicrobial activity in the tissue extracts of five species of cowries cypraea spp. (Mollusca: Gastropoda) and an ascidian didemnum psammathodes (Tunicata: Didemnidae)
5. Purification and characterization of an antibacterial and antineoplastic protein secretion of a sea hare, Aplysia juliana10.1016/0041-0101(89)90058-5 ↗
6. The isolation and structure of a remarkable marine animal antineoplastic constituent: dolastatin 1010.1021/ja00256a070 ↗
7. Study on cephalopod’s ink for anti-retroviral activity
8. Myticin, a novel cysteine-rich antimicrobial peptide isolated from haemocytes and plasma of the mussel Mytilus galloprovincialis10.1046/j.1432-1327.1999.00654.x ↗
9. Tachyplesin, a class of antimicrobial peptide from the hemocytes of the horseshoe crab (Tachypleus tridentatus). Isolation and chemical structure10.1016/S0021-9258(18)37448-9 ↗
10. Antimicrobial peptides of multicellular organisms10.1038/415389a ↗
11. PenBase, the shrimp antimicrobial peptide penaeidin database: sequence-based classification and recommended nomenclature10.1016/j.dci.2005.04.003 ↗
12. Putative antiviral activity in hemolymph from adult Pacific oysters, Crassostrea gigas {UR} {-://WOS:000229819900008}10.1016/j.antiviral.2005.03.003 ↗
13. NMR structure of mussel mytilin, and antiviral-antibacterial activities of derived synthetic peptides10.1016/j.dci.2007.05.006 ↗
14. Kuppusamy, A., and Ulagesan, S. (2016). Antimicrobial activity of protein hydrolysate from marine molluscs babylonia spirata (Linnaeus, 1758). J. Appl. Pharm. Sci., 6.10.7324/JAPS.2016.60711 ↗
15. Simultaneous determination of allantoin and glycolic acid in snail mucus and cosmetic creams with high performance liquid chromatography and ultraviolet detection10.1016/j.chroma.2013.10.086 ↗
16. Screening of Antimicrobials from Caribbean Sea Animals and Isolation of Bactericidal Proteins from the Littoral Mollusk Cenchritis muricatus10.1007/s00284-012-0096-5 ↗
17. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding10.1016/0003-2697(76)90527-3 ↗
18. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T410.1038/227680a0 ↗
19. Antibiotic susceptibility testing by a standardized single disk method10.1093/ajcp/45.4_ts.493 ↗
20. Discovery and characterization of two isoforms of moronecidin, a novel antimicrobial peptide from hybrid striped bass10.1074/jbc.M109173200 ↗
21. Screening of Antibacterial Activities of Marine Gastropod Hemifusus Pugilinus10.19026/crjbs.5.5472 ↗
22. Antimicrobial activity of peptides from the hemolymph of Helix lucorum snails
23. Assessment of Antimicrobial Activity and Healing Potential of Mucous Secretion of Achatina fulica10.4067/S0717-95022012000200001 ↗
24. Antipathogenic activity of freshwater Gastropod Pila virens (Lamarck, 1822) from Lower Grand Anaicut Reservoir, Tamilnadu