journal article·2022-02-17Open access

Extraction of Bioactive Compounds from Ulva lactuca

Sofia Pappou, Maria Myrto Dardavila, Maria G. Savvidou, Vasiliki Louli, Kostis Magoulas, Epaminondas Voutsas

Applied Sciences· Volume 12· Issue 4

Abstract

Macroalgae Ulva lactuca, has been employed as a natural source for the production of extracts with potent bioactivity. The biochemical characterization showed that the macroalgae biomass contains a remarkable amount of the polysaccharide Ulvan (49.9 wt%) which is a valuable chemical compound well known for its benefits in human health. Four nontoxic solvents, water, ethyl acetate, ethanol, and an ethanol/water mixture (70:30 v/v) were examined for their recovery efficiency of total carotenoid and phenolic contents. Experimental results showed that the aqueous mixture of ethanol was the most efficient solvent in the recovery of bioactive compounds with extraction yield of 10–15% dw. The effect of extraction parameters, namely time, temperature, and the ratio of biomass to solvent, on the carotenoid and phenolic compounds’ content, antioxidant activity, and extraction yield, was investigated, using the ethanol/water mixture as a solvent. The extract obtained under 60 °C, 3 h of extraction time and 1:10 biomass to solvent mass ratio showed the highest antioxidant activity. This extract maintained its antioxidant capacity almost stable for five days of storage under cool and dark conditions. Finally, specific phenolic and carotenoid compounds in the U. lactuca extracts were identified using the High-Performance Liquid Chromatography (HPLC) technique.
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Research context

OpenAlex topics: Seaweed-derived Bioactive Compounds · Phytochemical and Pharmacological Studies · Medicinal plant effects and applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Laboratory of Thermodynamics and Transport Phenomena, Zografou Campus, School of Chemical Engineering, National Technical University of Athens, 9 Iroon Polytechniou Str., 15780 Athens, Greece

Department of Marine Sciences, University of Aegean, University Hill, Lesvos Island, 81100 Mytilene, Greece

Funding

European Union · Greek national funds through the Operational Program Competitiveness, Entrepreneurship and Innovation

References (48)

Show source-provided references
1. Feasibility study of Ulva sp. (Chlorophyta) intensive cultivation in a coastal area of the Eastern Mediterranean Sea10.1002/bbb.1995 ↗
2. World seaweed utilization: A summary10.4172/2155-9546.S1.002 ↗
3. Biorefinery of the macroalgae Ulva lactuca: Extraction of proteins and carbohydrates by mild disintegration10.1007/s10811-017-1319-8 ↗
4. Dominguez, H., and Loret, E. (2019). Ulva lactuca, a source of troubles and potential riches. Mar. Drugs, 17.10.3390/md17060357 ↗
5. Biochemical components of three marine macroalgae (Padina pavonica, Ulva lactuca and Taonia atomaria) from the Levantine Sea coast of Antalya, Turkey
6. Morais, T., Cotas, J., Pacheco, D., and Pereira, L. (2021). Seaweeds compounds: An Eco sustainable source of cosmetic ingredients?. Cosmetics, 8.10.3390/cosmetics8010008 ↗
7. Main bioactive phenolic compounds in marine algae and their mechanisms of action supporting potential health benefits10.1016/j.foodchem.2020.128262 ↗
8. Commercial applications of microalgae10.1263/jbb.101.87 ↗
9. Elderberry as potential source of antioxidants. Characterization, optimization of extraction parameters and bioactive properties10.1016/j.foodchem.2020.127266 ↗
10. Process optimization for recovery of carotenoids from tomato waste10.1016/j.foodchem.2011.05.015 ↗
11. Solvent polarity effect on the composition of biologically active substances, UV spectral characteristics, and antibacterial activity of Euphrasia brevipila herb extracts10.1007/s11094-011-0542-x ↗
12. Green solvents for the extraction of high added-value compounds from agri-food waste10.1007/s12393-019-09206-y ↗
13. Effect of extraction method and solvent system on the phenolic content and antioxidant activity of selected macro-and microalgae extracts
14. He, J., Xu, Y., Chen, H., and Sun, P. (2016). Extraction, structural characterization, and potential antioxidant activity of the polysaccharides from four seaweeds. Int. J. Mol. Sci., 17.10.3390/ijms17121988 ↗
15. Characterization of ulvan extracts to assess the effect of different steps in the extraction procedure10.1016/j.carbpol.2011.12.041 ↗
16. Dietary fiber, amino acid, fatty acid and tocopherol contents of the edible seaweeds Ulva lactuca and Durvillaea antarctica10.1016/j.foodchem.2005.07.027 ↗
17. Effect of extraction conditions on the yield and purity of ulvan extracted from Ulva lactuca10.1016/j.foodhyd.2012.11.013 ↗
18. Impact of extraction procedures on the chemical, rheological and textural properties of ulvan from Ulva lactuca of Tunisia coast10.1016/j.foodhyd.2014.02.002 ↗
19. Effect of extraction procedures on structural, thermal and antioxidant properties of ulvan from Ulva lactuca collected in Monastir coast10.1016/j.ijbiomac.2017.07.141 ↗
20. Isolation, structure, and surfactant properties of polysaccharides from Ulva lactuca L. from South China Sea10.1016/j.ijbiomac.2015.05.031 ↗
21. Natural preservative ingredient from marine alga10.1111/j.1365-2621.2009.01926.x ↗
22. Nutrient and Nutraceutical Potentials of Seaweed Biomass Ulva lactuca and Kappaphycus alvarezii
23. Total phenolic compounds, radical scavenging and metal chelation of extracts from Icelandic seaweeds10.1016/j.foodchem.2009.02.041 ↗
24. A simple method for the isolation and purification of total lipids from animal tissues10.1016/S0021-9258(18)64849-5 ↗
25. Extraction of proteins from two marine macroalgae, Ulva sp. and Gracilaria sp., for food application, and evaluating digestibility, amino acid composition and antioxidant properties of the protein concentrates10.1016/j.foodhyd.2018.07.047 ↗
26. Sulfated polysaccharides from marine green algae Ulva conglobata and their anticoagulant activity10.1007/s10811-005-9008-4 ↗
27. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents10.5344/ajev.1965.16.3.144 ↗
28. Association of Official Analytical Chemists (AOAC) (1995). Official Methods of Analysis of the Association of Official Analytical Chemists International, The Association of Official Analytical Chemists. [16th ed.].
29. Use of a free radical method to evaluate antioxidant activity10.1016/S0023-6438(95)80008-5 ↗
30. HPLC-PDA-MS/MS as a strategy to characterize and quantify natural pigments from microalgae
31. Antioxidant activity of phenolics extracted from Olea europaea L. leaves10.1016/S0308-8146(99)00221-6 ↗
32. Biorefinery of the green seaweed Ulva lactuca to produce animal feed, chemicals and biofuels10.1007/s10811-016-0842-3 ↗
33. Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D.G., and Lightfoot, D.A. (2017). Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants, 6.10.3390/plants6040042 ↗
34. Isobaric enthalpies of vaporization of water, methanol, ethanol, propan-2-ol and their mixtures10.1016/0021-9614(82)90020-9 ↗
35. Effective extraction of carotenoids from brown seaweeds and vegetable leaves with edible oils10.1016/j.ifset.2020.102302 ↗
36. Process optimization for extraction of carotenoids from shrimp waste with vegetable oils10.1016/j.biortech.2004.09.018 ↗
37. Optimization of antioxidant activity and phenolic compound extraction conditions from red seaweed (Laurencia obtuse)10.1080/10498850.2013.868844 ↗
38. Effect of extraction parameters on the carotenoid recovery from tomato waste10.1111/j.1365-2621.2010.02496.x ↗
39. Comparative study of the extraction methods for recovery of carotenoids from algae: Extraction kinetics and effect of different extraction parameters10.1002/jctb.4461 ↗
40. Effects of extraction time, temperature and solvent on concentration and antioxidant activity of grape marc phenolics10.1016/j.jfoodeng.2006.10.021 ↗
41. Ummat, V., Tiwari, B., Jaiswal, A., Condon, K., Garcia-Vaquero, M., O’Doherty, J., O’Donnell, C., and Rajauria, G. (2020). Optimisation of Ultrasound Frequency, Extraction Time and Solvent for the Recovery of Polyphenols, Phlorotannins and Associated Antioxidant Activity from Brown Seaweeds. Mar. Drugs, 18.10.3390/md18050250 ↗
42. Kinetic behavior of DPPH radical scavenging activity of tomato waste extracts10.2298/JSC120410065S ↗
43. HPLC detection and antioxidant capacity determination of brown, red and green algal pigments in seaweed extracts
44. Torregrosa-Crespo, J., Montero, Z., Fuentes, J.L., García-Galbis, M.R., Garbayo, I., Vílchez, C., and Martínez-Espinosa, R.M. (2018). Exploring the Valuable Carotenoids for the Large-Scale Production by Marine Microorganisms. Mar. Drugs, 16.10.3390/md16060203 ↗
45. Monitoring the antioxidant activities by extracting the polyphenolic contents of algae collected from the Bosphorus10.1016/j.marpolbul.2019.02.059 ↗
46. Phenolic compounds and antioxidant activities of selected species of seaweeds from Danish coast10.1016/j.foodchem.2012.10.078 ↗
47. Phenolic content of brown algae (Pheophyceae) species: Extraction, identification, and quantification
48. The possible role of the seaweed Ulva fasciata on ameliorating hyperthyroidism-associated heart inflammations in a rat model10.1007/s11356-020-11036-z ↗