journal article·2021-11-11Open access

Methods of Analyzing Microsized Plastics in the Environment

Hyunjeong Woo, Kangmin Seo, Yonghyun Choi, Jiwon Kim, Masayoshi Tanaka, Keunheon Lee, Jonghoon Choi

Applied Sciences· Volume 11· Issue 22

Abstract

Microplastics are found in various environments with the increasing use of plastics worldwide. Several methods have been developed for the sampling, extraction, purification, identification, and quantification of microplastics in complex environmental matrices. This study intends to summarize recent research trends on the subject. Large microplastic particles can be sorted manually and identified through chemical analysis; however, sample preparation for small microplastic analysis is usually more difficult. Microplastics are identified by evaluating the physical and chemical properties of plastic particles separated through extraction and washing steps from a mixture of inorganic and organic particles. This identification has a high risk of producing false-positive and false-negative results in the analysis of small microplastics. Currently, a combination of physical (e.g., microscopy), chemical (e.g., spectroscopy), and thermal analyses is widely used. We aim to summarize the best strategies for microplastic analysis by comparing the strengths and limitations of each identification method.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Microplastics and Plastic Pollution · Recycling and Waste Management Techniques

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

School of Integrative Engineering, Chung-Ang University, Seoul 06974, Korea

Department of Chemical Science and Engineering, Tokyo Institute of Technology, Tokyo 152-8552, Japan

Humas, Daejeon 34224, Korea

Funding

Chung-Ang University

References (86)

Show source-provided references
1. Synthetic Polymer Contamination in Bottled Water10.3389/fchem.2018.00407 ↗
2. Plasticizer Degradation by Marine Bacterial Isolates: A Proteogenomic and Metabolomic Characterization10.1021/acs.est.9b05228 ↗
3. An airborne remote sensing case study of synthetic hydrocarbon detection using short wave infrared absorption features identified from marine-harvested macro- and microplastics10.1016/j.rse.2017.11.023 ↗
4. From nanoplastic to microplastic: A bibliometric analysis on the presence of plastic particles in the environment10.1016/j.marpolbul.2020.111926 ↗
5. Legislation to limit the environmental plastic and microplastic pollution and their influence on human exposure10.1016/j.envpol.2021.117708 ↗
6. Valorization of disposable COVID-19 mask through the thermo-chemical process10.1016/j.cej.2020.126658 ↗
7. Estimating marine plastic pollution from COVID-19 face masks in coastal regions10.1016/j.marpolbul.2021.112419 ↗
8. The influence of weirs on microplastic fate in the riverine environment (case study: Jeneberang River, Makassar City, Indonesia)10.1088/1755-1315/763/1/012054 ↗
9. Effect of microplastic size on the adsorption behavior and mechanism of triclosan on polyvinyl chloride10.1016/j.envpol.2019.113104 ↗
10. Microplastics in different tissues of fish and prawn from the Musa Estuary, Persian Gulf10.1016/j.chemosphere.2018.04.076 ↗
11. Microplastic abundance, distribution and composition in the mid-west Pacific Ocean10.1016/j.envpol.2020.114125 ↗
12. Quantitative and qualitative determination of microplastics in oyster, seawater and sediment from the coastal areas in Zhuhai, China10.1016/j.marpolbul.2021.112000 ↗
13. Microplastic in bottled natural mineral water - literature review and considerations on exposure and risk assessment10.1080/19440049.2018.1543957 ↗
14. Weis, J.S. (2020). Aquatic Microplastic Research—A Critique and Suggestions for the Future. Water, 12.10.3390/w12051475 ↗
15. Albano, M., Panarello, G., Di Paola, D., Capparucci, F., Crupi, R., Gugliandolo, E., Spanò, N., Capillo, G., and Savoca, S. (2021). The Influence of Polystyrene Microspheres Abundance on Development and Feeding Behavior of Artemia salina (Linnaeus, 1758). Appl. Sci., 11.10.3390/app11083352 ↗
16. Plastics occurrence in juveniles of Engraulis encrasicolus and Sardina pilchardus in the Southern Tyrrhenian Sea10.1016/j.scitotenv.2020.137457 ↗
17. Role of benzophenone-3 additive in chronic toxicity of polyethylene microplastic fragments to Daphnia magna10.1016/j.scitotenv.2021.149638 ↗
18. Turning microplastics into nanoplastics through digestive fragmentation by Antarctic krill10.1038/s41467-018-03465-9 ↗
19. Effects of particle size and solution chemistry on Triclosan sorption on polystyrene microplastic10.1016/j.chemosphere.2019.05.116 ↗
20. Microplastic analysis-are we measuring the same? Results on the first global comparative study for microplastic analysis in a water sample10.1007/s00216-019-02311-1 ↗
21. Marine litter within the European Marine Strategy Framework Directive10.1093/icesjms/fst122 ↗
22. Microplastic particles in the Persian/Arabian Gulf - A review on sampling and identification10.1016/j.marpolbul.2020.111100 ↗
23. Promising techniques and open challenges for microplastic identification and quantification in environmental matrices10.1007/s00216-019-01763-9 ↗
24. Validated spreadsheet for the identification of PE, PET, PP and PS microplastics by micro-ATR-FTIR spectra with known uncertainty10.1016/j.talanta.2021.122624 ↗
25. Microplastic Detection in Soil Amended With Municipal Solid Waste Composts as Revealed by Transmission Electronic Microscopy and Pyrolysis/GC/MS10.3389/fsufs.2018.00081 ↗
26. Probing the aging processes and mechanisms of microplastic under simulated multiple actions generated by discharge plasma10.1016/j.jhazmat.2020.122956 ↗
27. Kniggendorf, A.K., Wetzel, C., and Roth, B. (2019). Microplastics Detection in Streaming Tap Water with Raman Spectroscopy. Sensors, 19.10.3390/s19081839 ↗
28. Study of Analysis Method on Microplastic Identification in Bottled Drinking Water10.1002/masy.201900195 ↗
29. Separation and identification of microplastics in marine organisms by TGA-FTIR-GC/MS: A case study of mussels from coastal China10.1016/j.envpol.2020.115946 ↗
30. Lost, but Found with Nile Red: A Novel Method for Detecting and Quantifying Small Microplastics (1 mm to 20 mum) in Environmental Samples10.1021/acs.est.7b04512 ↗
31. Garaba, S., and Dierssen, H. (2017). Spectral reference library of 11 types of virgin plastic pellets common in marine plastic debris. Data Set, Available online: https://ecosis.org/package/spectral-reference-library-of-11-types-of-virgin-plastic-pellets-common-in-marine-plastic-debris.
32. Nondestructive Extraction and Identification of Microplastics from Freshwater Sport Fish Stomachs10.1021/acs.est.9b05072 ↗
33. When Good Intentions Go Bad-False Positive Microplastic Detection Caused by Disposable Gloves10.1021/acs.est.0c03742 ↗
34. The Hidden Microplastics: New Insights and Figures from the Thorough Separation and Characterization of Microplastics and of Their Degradation Byproducts in Coastal Sediments10.1021/acs.est.8b01487 ↗
35. The effect of urban point source contamination on microplastic levels in water and organisms in a cold-water stream10.1002/lol2.10138 ↗
36. Microplastic prevalence in the beaches of Puducherry, India and its correlation with fishing and tourism/recreational activities10.1016/j.marpolbul.2019.07.066 ↗
37. Microplastics in the environment: Challenges in analytical chemistry—A review10.1016/j.aca.2018.02.043 ↗
38. Automated thermal extraction-desorption gas chromatography mass spectrometry: A multifunctional tool for comprehensive characterization of polymers and their degradation products10.1016/j.chroma.2019.01.033 ↗
39. Microplastic content of Kutum fish, Rutilus frisii kutum in the southern Caspian Sea10.1016/j.scitotenv.2020.141542 ↗
40. Bergmann, M., Gutow, L., and Klages, M. (2015). Methodology Used for the Detection and Identification of Microplastics—A Critical Appraisal. Marine Anthropogenic Litter, Springer International Publishing.10.1007/978-3-319-16510-3 ↗
41. Microplastic pollution in deposited urban dust, Tehran metropolis, Iran10.1007/s11356-017-9674-1 ↗
42. Microplastic Abundance and Composition in Western Lake Superior As Determined via Microscopy, Pyr-GC/MS, and FTIR10.1021/acs.est.7b05829 ↗
43. Identification methods in microplastic analysis: A review10.1039/C6AY02558G ↗
44. Effect of microplastics and microplastic-metal combinations on growth and chlorophyll a concentration of Chlorella vulgaris10.1016/j.scitotenv.2020.140479 ↗
45. Microplastic surface properties affect bacterial colonization in freshwater10.1002/jobm.201800174 ↗
46. Feasibility study on co-pyrolyzation of microplastic extraction in conventional sewage sludge for the cementitious application10.1016/j.matpr.2021.05.439 ↗
47. Preparation and analysis of standards containing microfilaments/microplastic with fibre shape10.1016/j.chemosphere.2020.129410 ↗
48. Prevalence and physicochemical characteristics of microplastics in the sediment and water of Hashilan Wetland, a national heritage in NW Iran10.1016/j.eti.2021.101782 ↗
49. Experimental development of a new protocol for extraction and characterization of microplastics in fish tissues: First observations in commercial species from Adriatic Sea10.1016/j.marenvres.2015.06.014 ↗
50. New sampling method to improve the reliability of FTIR analysis for Self-Compacting Concrete10.1016/j.conbuildmat.2018.03.216 ↗
51. Validation of ATR FT-IR to identify polymers of plastic marine debris, including those ingested by marine organisms10.1016/j.marpolbul.2017.12.061 ↗
52. Veerasingam, S., Ranjani, M., Venkatachalapathy, R., Bagaev, A., Mukhanov, V., Litvinyuk, D., Mugilarasan, M., Gurumoorthi, K., Guganathan, L., and Aboobacker, V.M. (2020). Contributions of Fourier transform infrared spectroscopy in microplastic pollution research: A review. Crit. Rev. Environ. Sci. Technol., 1–63.10.1080/10643389.2020.1807450 ↗
53. Characterizing microplastic size and morphology of photodegraded polymers placed in simulated moving water conditions10.1039/C9EM00475K ↗
54. A novel method for purification, quantitative analysis and characterization of microplastic fibers using Micro-FTIR10.1016/j.chemosphere.2019.124564 ↗
55. Identification of microplastic in effluents of waste water treatment plants using focal plane array-based micro-Fourier-transform infrared imaging10.1016/j.watres.2016.11.015 ↗
56. Vianello, A. (2020). A Journey into Microplastic Analysis Using FTIR Spectroscopy. [Ph.D. Thesis, Aalborg University].
57. Olesen, K., van Alst, N., Simon, M., Vianello, A., Liu, F., and Vollertsen, J. (2017). Analysis of Microplastics using FTIR Imaging Application Note. Agil. Appl. Note Environ., 5991-8271EN. Available online: https://www.researchgate.net/publication/332083136_Analysis_of_Microplastics_using_FTIR_Imaging_Application_Note.
58. Quantification of microplastic mass and removal rates at wastewater treatment plants applying Focal Plane Array (FPA)-based Fourier Transform Infrared (FT-IR) imaging10.1016/j.watres.2018.05.019 ↗
59. Reference database design for the automated analysis of microplastic samples based on Fourier transform infrared (FTIR) spectroscopy10.1007/s00216-018-1156-x ↗
60. In Situ Migration Analysis and Diffusion Coefficient Determination of Bio-Based Plasticizer From NBR Using FTIR-ATR and Estimation of Migrated Plasticizer Contents by TGA Analysis10.1002/masy.201800158 ↗
61. Implementation of an open source algorithm for particle recognition and morphological characterisation for microplastic analysis by means of Raman microspectroscopy10.1039/C9AY01245A ↗
62. Thermokinetic and TG/DSC-FTIR study of pea waste biomass pyrolysis10.1016/j.applthermaleng.2018.03.050 ↗
63. Identification and quantitation of semi-crystalline microplastics using image analysis and differential scanning calorimetry10.1007/s11356-018-1846-0 ↗
64. Fast and easy quantification of semi-crystalline microplastics in exemplary environmental matrices by differential scanning calorimetry (DSC)10.1016/j.cej.2021.129941 ↗
65. Comparison of the thermal degradation behaviors and kinetics of palm oil waste under nitrogen and air atmosphere in TGA-FTIR with a complementary use of model-free and model-fitting approaches10.1016/j.jaap.2018.04.002 ↗
66. Determination of microplastic polyethylene (PE) and polypropylene (PP) in environmental samples using thermal analysis (TGA-DSC)10.1016/j.scitotenv.2016.06.017 ↗
67. Microplastics in wastewater treatment plants: Detection, occurrence and removal10.1016/j.watres.2018.12.050 ↗
68. Fast identification of microplastics in complex environmental samples by a thermal degradation method10.1016/j.chemosphere.2017.02.010 ↗
69. Wastewater treatment plants as a source of plastics in the environment: A review of occurrence, methods for identification, quantification and fate10.1039/C9EW00428A ↗
70. Environmental fate and impacts of microplastics in soil ecosystems: Progress and perspective10.1016/j.scitotenv.2019.134841 ↗
71. Photocatalytic aging process of Nano-TiO2 coated polypropylene microplastics: Combining atomic force microscopy and infrared spectroscopy (AFM-IR) for nanoscale chemical characterization10.1016/j.jhazmat.2020.124159 ↗
72. Nanoscale infrared, thermal and mechanical properties of aged microplastics revealed by an atomic force microscopy coupled with infrared spectroscopy (AFM-IR) technique10.1016/j.scitotenv.2020.140944 ↗
73. Methods for sampling and detection of microplastics in water and sediment: A critical review10.1016/j.trac.2018.10.029 ↗
74. Nile Red lifetime reveals microplastic identity10.1039/D0EM00348D ↗
75. Microplastic identification and quantification from organic rich sediments: A validated laboratory protocol10.1016/j.envpol.2020.114298 ↗
76. Al-Azzawi, M.S.M., Kefer, S., Weißer, J., Reichel, J., Schwaller, C., Glas, K., Knoop, O., and Drewes, J.E. (2020). Validation of Sample Preparation Methods for Microplastic Analysis in Wastewater Matrices—Reproducibility and Standardization. Water, 12.10.3390/w12092445 ↗
77. Effects of chemical pretreatments on microplastic extraction in sewage sludge and their physicochemical characteristics10.1016/j.watres.2019.115379 ↗
78. Identification and quantification of microplastics in table sea salts using micro-NIR imaging methods10.1039/C8AY00125A ↗
79. High-throughput NIR spectroscopic (NIRS) detection of microplastics in soil10.1007/s11356-018-2180-2 ↗
80. Predicting soil microplastic concentration using vis-NIR spectroscopy10.1016/j.scitotenv.2018.09.101 ↗
81. Effects of advanced oxidation processes on leachates and properties of microplastics10.1016/j.jhazmat.2021.125342 ↗
82. SThM-based local thermomechanical analysis: Measurement intercomparison and uncertainty analysis10.1016/j.ijthermalsci.2020.106502 ↗
83. Detection of artificial cellulose microfibers in Boops boops from the northern coasts of Sicily (Central Mediterranean)10.1016/j.scitotenv.2019.07.148 ↗
84. Microfibres from apparel and home textiles: Prospects for including microplastics in environmental sustainability assessment10.1016/j.scitotenv.2018.10.166 ↗
85. Synthetic microfibers in the marine environment: A review on their occurrence in seawater and sediments10.1016/j.marpolbul.2017.11.070 ↗
86. Marine environment microfiber contamination: Global patterns and the diversity of microparticle origins10.1016/j.envpol.2018.02.062 ↗