DataVXApplied SciencesPaper
journal article·2019-02-25Open access

Breast Cancer Diagnosis by Surface-Enhanced Raman Scattering (SERS) of Urine

Vlad Moisoiu, Andreea Socaciu, Andrei Stefancu, Stefania D. Iancu, Imre Boros, Cristian D. Alecsa, Claudiu Rachieriu, Angelica R. Chiorean, Daniela Eniu, Nicolae Leopold, Carmen Socaciu, Dan T. Eniu

Applied Sciences· Volume 9· Issue 4
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Source abstract

Abstract

Background: There is an ongoing research for breast cancer diagnostic tools that are cheaper, more accurate and more convenient than mammography. Methods: In this study, we employed surface-enhanced Raman scattering (SERS) for analysing urine from n = 53 breast cancer patients and n = 22 controls, with the aim of discriminating between the two groups using multivariate data analysis techniques such as principal component analysis—linear discriminant analysis (PCA-LDA). The SERS spectra were acquired using silver nanoparticles synthesized by reduction with hydroxylamine hydrochloride, which were additionally activated with Ca2+ 10−4 M. Results: The addition of Ca(NO3)2 10−4 M promoted the specific adsorption to the metal surface of the anionic purine metabolites such as uric acid, xanthine and hypoxanthine. Moreover, the SERS spectra of urine were acquired without any filtering or processing step for removing protein traces and other contaminants. Using PCA-LDA, the SERS spectra of urine from breast cancer patients were classified with a sensitivity of 81%, a specificity of 95% and an overall accuracy of 88%. Conclusion: The results of this preliminary study contribute to the translation of SERS in the clinical setting and highlight the potential of SERS as a novel screening strategy for breast cancer.
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Research context

Nanomaterials

OpenAlex topics: Spectroscopy Techniques in Biomedical and Chemical Research · Identification and Quantification in Food · Biosensors and Analytical Detection

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Faculty of Physics, Babeș-Bolyai University, 400084 Cluj-Napoca, Romania

Faculty of Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400349 Cluj-Napoca, Romania

MEDISYN Clinic, 400474 Cluj-Napoca, Romania

MEDFUTURE Research Center for Advanced Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400000 Cluj-Napoca, Romania

IMOGEN Medical Research Institute, County Clinical Emergency Hospital, 400012 Cluj-Napoca, Romania

Faculty of Mathematics and Computer Science, Babeș-Bolyai University, 400084 Cluj-Napoca, Romania

Tiberiu Popoviciu Institute of Numerical Analysis, 400320 Cluj-Napoca, Romania

Department of Radiology, Iuliu Hatieganu University of Medicine and Pharmacy, 400349 Cluj-Napoca, Romania

Department of Biophysics, Iuliu Hatieganu University of Medicine and Pharmacy, 400349 Cluj-Napoca, Romania

Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania

BIODIATECH Research Centre for Applied Biotechnology, SC Proplanta, 400478 Cluj-Napoca, Romania

Department of Surgical and Gynecological Oncology, Ion Chiricuta Oncologic Institute, 400015 Cluj-Napoca, Romania

Funding

European Regional Development Fund

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Show source-provided references
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