journal article·2022-04-27Open access

Microalgal Proteins and Bioactives for Food, Feed, and Other Applications

Simona Lucakova, Irena Branyikova, Maria Hayes

Applied Sciences· Volume 12· Issue 9

Abstract

Microalgae are a known source of proteins, prebiotics, lipids, small molecules, anti-oxidants and bioactives with health benefits that can be harnessed for the development of functional foods, feeds, cosmeceuticals and pharmaceuticals. This review collates information on the supply, processing costs, target markets and value of microalgae, as well as microalgal proteins, lipids, vitamins and minerals. It discusses the potential impact that microalgae could have on global food and feed supply and highlights gaps that exist with regards to the use of microalgal proteins and ingredients as foods and supplements.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Algal biology and biofuel production

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Biotechnology, University of Chemistry and Technology, Technicka 5, 166 28 Prague, Czech Republic

Institute of Chemical Process Fundamentals of the Czech Academy of Sciences, Rozvojova 135/1, 165 02 Prague, Czech Republic

Food BioSciences Department, Teagasc Food Research Centre, Ashtown, 15 Dublin, Ireland

Funding

EU Interreg, North West Europe (NWE) · Erasmus

References (100+)

Show source-provided references
1. United Nations (2019). World Population Prospects 2019: Highlights, United Nations.
2. WHO (2021, December 20). Malnutrition. Available online: https://www.who.int/news-room/fact-sheets/detail/malnutrition.
3. Microalgae as feed ingredients for livestock production and meat quality: A review10.1016/j.livsci.2017.09.020 ↗
4. Genetic diversity and evolution in eukaryotic phytoplankton: Revelations from population genetic studies
5. Darzins, A., Pienkos, P., and Edye, L. (2010). Current Status and Potential for Algal Biofuels Production, National Renewable Energy Laboratory. A Report to IEA Bioenergy Task 39.
6. Microalgal biofactories: A promising approach towards sustainable omega-3 fatty acid production10.1186/1475-2859-11-96 ↗
7. Biofuels from microalgae10.1021/bp070371k ↗
8. Eltanahy, E., and Torky, A. (2021). Chapter 1 Microalgae as Cell Factories: Food and Feed-grade High-value Metabolites. Microalgal Biotechnology: Recent Advances, Market Potential, and Sustainability, The Royal Society of Chemistry.10.1039/9781839162473-00001 ↗
9. Tecuitlatl; A Glimpse of Aztec Food Technology10.1038/211341a0 ↗
10. Spirulina, the edible microorganism10.1128/mr.47.4.551-578.1983 ↗
11. Etymology of the Genus Name Nostoc (Cyanobacteria)
12. Chinese studies on the edible blue-green alga, Nostoc flagelliforme: A review10.1023/A:1008014424247 ↗
13. Physiological properties and genetic analysis related to exopolysaccharide (EPS) production in the fresh-water unicellular cyanobacterium Aphanothece sacrum (Suizenji Nori)10.2323/jgam.2018.04.004 ↗
14. Burlew, J.S. (1953). Algal Culture from Laboratory to Pilot Plant, Carnegie Institution of Washington.
15. Appropriate biotechnological systems in the arid environment
16. Enzing, C.M., Ploeg, M., Barbosa, M.J., and Sijtsma, L. (2014). Microalgae-Based Products for the Food and Feed Sector: An Outlook for Europe, Joint Research Centre.
17. Current Status of the Algae Production Industry in Europe: An Emerging Sector of the Blue Bioeconomy
18. Exploring the metabolic and physiological diversity of native microalgal strains (Chlorophyta) isolated from tropical freshwater reservoirs10.1016/j.algal.2017.10.021 ↗
19. Effective role of medium supplementation in microalgal lipid accumulation10.1002/bit.26548 ↗
20. Microalgae proteins: Production, separation, isolation, quantification, and application in food and feed10.1080/10408398.2020.1768046 ↗
21. Kratzer, R., and Murkovic, M. (2021). Food Ingredients and Nutraceuticals from Microalgae: Main Product Classes and Biotechnological Production. Foods, 10.10.3390/foods10071626 ↗
22. Trends in Microalgae Incorporation into Innovative Food Products with Potential Health Benefits10.3389/fnut.2018.00058 ↗
23. Habib, M.A.B. (2008). Review on Culture, Production and Use of Spirulina as Food for Humans and Feeds for Domestic Animals and Fish, FAO.
24. FAO Expert Consultation (2013). Dietary protein quality evaluation in human nutrition. FAO Food Nutr. Pap., 92, 1–66.
25. Wang, Y., Tibbetts, S.M., and McGinn, P.J. (2021). Microalgae as Sources of High-Quality Protein for Human Food and Protein Supplements. Foods, 10.10.3390/foods10123002 ↗
26. Apparent digestibility coefficients (ADCs) of intact-cell marine microalgae meal (Pavlova sp. 459) for juvenile Atlantic salmon (Salmo salar L.)10.1016/j.aquaculture.2021.737236 ↗
27. Protein and amino acid digestibility of (15)N Spirulina in rats10.1007/s00394-020-02368-0 ↗
28. Perforation patterns in the peptidoglycan wall of filamentous cyanobacteria10.1046/j.1529-8817.2000.99040.x ↗
29. Wang, Y., Tibbetts, S.M., Berrue, F., McGinn, P.J., MacQuarrie, S.P., Puttaswamy, A., Patelakis, S., Schmidt, D., Melanson, R., and MacKenzie, S.E. (2020). A Rat Study to Evaluate the Protein Quality of Three Green Microalgal Species and the Impact of Mechanical Cell Wall Disruption. Foods, 9.10.3390/foods9111531 ↗
30. Classification of Chlorella strains by cell wall sugar composition10.1016/0031-9422(88)83025-5 ↗
31. Evaluation of Chlorella (Chlorophyta) as Source of Fermentable Sugars via Cell Wall Enzymatic Hydrolysis10.4061/2011/405603 ↗
32. Levine, I.A., and Fleurence, J. (2018). Chapter 9—Microalgae in Medicine and Human Health: A Historical Perspective. Microalgae in Health and Disease Prevention, Academic Press.
33. Ultrastructural and chemical changes in the cell wall of Haematococcus pluvialis (Volvocales, Chlorophyta) during aplanospore formation10.1017/S0967026202003669 ↗
34. The amino-acid and sugar composition of 16 species of microalgae used in mariculture10.1016/0022-0981(91)90007-J ↗
35. Determination of biomass dry weight of marine microalgae10.1023/A:1007914806640 ↗
36. Effects of different photoperiod and trophic conditions on biomass, protein and lipid production by the marine alga Nannochloropsis gaditana at optimal concentration of desalination concentrate10.1016/j.biortech.2016.11.004 ↗
37. Ultrastructure and composition of the Nannochloropsis gaditana cell wall10.1128/EC.00183-14 ↗
38. Identification of lipid and saccharide constituents of whole microalgal cells by 13C solid-state NMR10.1016/j.bbamem.2014.07.017 ↗
39. Digestibility of Schizochytrium sp. whole cell biomass by Atlantic salmon (Salmo salar)10.1016/j.aquaculture.2020.736156 ↗
40. Cell wall composition and synthesis via Golgi-directed scale formation in the marine eucaryote, Schizochytrium aggregatum, with a note on Thraustochytrium sp.10.1007/BF00414512 ↗
41. Enzymatic and acid hydrolysis of Tetraselmis suecica for polysaccharide characterization10.1016/j.biortech.2014.09.048 ↗
42. Franca-Oliveira, G., Fornari, T., and Hernández-Ledesma, B. (2021). A Review on the Extraction and Processing of Natural Source-Derived Proteins through Eco-Innovative Approaches. Processes, 9.10.3390/pr9091626 ↗
43. Roy, U.K., Nielsen, B.V., and Milledge, J.J. (2021). Antioxidant Production in Dunaliella. Appl. Sci., 11.10.3390/app11093959 ↗
44. Echave, J., Fraga-Corral, M., Garcia-Perez, P., Popović-Djordjević, J., Avdović, E.H., Radulović, M., Xiao, J., Prieto, M.A., and Simal-Gandara, J. (2021). Seaweed Protein Hydrolysates and Bioactive Peptides: Extraction, Purification, and Applications. Mar. Drugs, 19.10.3390/md19090500 ↗
45. Sathya, R., MubarakAli, D., MohamedSaalis, J., and Kim, J.-W. (2021). A Systemic Review on Microalgal Peptides: Bioprocess and Sustainable Applications. Sustainability, 13.10.3390/su13063262 ↗
46. The physiological importance of the casein phosphopeptide calcium salts. II. Peroral calcium dosage of infants
47. Purification of novel anti-inflammatory peptides from enzymatic hydrolysate of the edible microalgal Spirulina maxima10.1016/j.jff.2013.05.001 ↗
48. Antioxidant Activity of Purified Active Peptide Derived from Spirulina platensis Enzymatic Hydrolysates10.21776/ub.rjls.2017.004.02.5 ↗
49. Separation and nanoencapsulation of antitumor polypeptide from Spirulina platensis10.1002/btpr.1769 ↗
50. Identification of antihypertensive peptides from peptic digest of two microalgae, Chlorella vulgaris and Spirulina platensis10.1007/s10126-001-0012-7 ↗
51. Protective effect of a novel antioxidative peptide purified from a marine Chlorella ellipsoidea protein against free radical-induced oxidative stress10.1016/j.fct.2012.04.022 ↗
52. Separation, antitumor activities, and encapsulation of polypeptide from Chlorella pyrenoidosa10.1002/btpr.1725 ↗
53. Li, Y., Aiello, G., Fassi, E.M.A., Boschin, G., Bartolomei, M., Bollati, C., Roda, G., Arnoldi, A., Grazioso, G., and Lammi, C. (2021). Investigation of Chlorella pyrenoidosa Protein as a Source of Novel Angiotensin I-Converting Enzyme (ACE) and Dipeptidyl Peptidase-IV (DPP-IV) Inhibitory Peptides. Nutrients, 13.10.3390/nu13051624 ↗
54. Chlorella 11-peptide inhibits the production of macrophage-induced adhesion molecules and reduces endothelin-1 expression and endothelial permeability10.3390/md11103861 ↗
55. Tejano, L.A., Peralta, J.P., Yap, E.E.S., Panjaitan, F.C.A., and Chang, Y.W. (2019). Prediction of Bioactive Peptides from Chlorella sorokiniana Proteins Using Proteomic Techniques in Combination with Bioinformatics Analyses. Int. J. Mol. Sci., 20.10.3390/ijms20071786 ↗
56. Antioxidant properties of a new antioxidative peptide from algae protein waste hydrolysate in different oxidation systems10.1016/j.biortech.2009.02.014 ↗
57. Antioxidant Peptide Purified from Enzymatic Hydrolysates of Isochrysis Zhanjiangensis and Its Protective Effect against Ethanol Induced Oxidative Stress of HepG2 Cells10.1007/s12257-018-0391-5 ↗
58. Angiotensin I-Converting Enzyme (ACE) Inhibitory Peptide Isolated from Biodiesel Byproducts of Marine Microalgae, Nannochloropsis oculata10.1166/jbmb.2013.1264 ↗
59. Purification and identification of novel angiotensin-I converting enzyme (ACE) inhibitory peptide from cultured marine microalgae (Nannochloropsis oculata) protein hydrolytes10.1007/s10811-013-9994-6 ↗
60. Protective effects of protein hydrolysate from marine microalgae Navicula incerta on ethanol-induced toxicity in HepG2/CYP2E1 cells10.1016/j.foodchem.2011.10.031 ↗
61. Characterization of growth and protein contents from microalgae Navicula incerta with the investigation of antioxidant activity of enzymatic hydrolysates10.1007/s10068-011-0025-6 ↗
62. Peptidomic strategy for purification and identification of potential ACE-inhibitory and antioxidant peptides in Tetradesmus obliquus microalgae10.1007/s00216-018-0925-x ↗
63. Microalgae: A potential sustainable commercial source of sterols10.1016/j.algal.2019.101772 ↗
64. Lee, J.M., Lee, H., Kang, S., and Park, W.J. (2016). Fatty Acid Desaturases, Polyunsaturated Fatty Acid Regulation, and Biotechnological Advances. Nutrients, 8.10.3390/nu8010023 ↗
65. Microalgae as rich source of polyunsaturated fatty acids10.1016/j.bcab.2019.01.017 ↗
66. Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor10.1002/bit.22033 ↗
67. n-3 PUFA productivity in chemostat cultures of microalgae
68. LED power efficiency of biomass, fatty acid, and carotenoid production in Nannochloropsis microalgae10.1016/j.biortech.2017.12.096 ↗
69. Increase of the yields of eicosapentaenoic and docosahexaenoic acids by the microalga Pavlova lutheri following random mutagenesis10.1002/bit.10451 ↗
70. Patel, A., Matsakas, L., Hrůzová, K., Rova, U., and Christakopoulos, P. (2019). Biosynthesis of Nutraceutical Fatty Acids by the Oleaginous Marine Microalgae Phaeodactylum tricornutum Utilizing Hydrolysates from Organosolv-Pretreated Birch and Spruce Biomass. Mar. Drugs, 17.10.3390/md17020119 ↗
71. Optimisation of docosahexaenoic acid production in batch cultivations by Crypthecodinium cohnii10.1016/S0168-1656(99)00071-1 ↗
72. Optimization of docosahexaenoic acid production by Schizochytrium limacinum SR2110.1007/s002530051139 ↗
73. Fatty acids profile of Spirulina platensis grown under different temperatures and nitrogen concentrations
74. Enhancing total fatty acids and arachidonic acid production by the red microalgae Porphyridium purpureum10.1186/s40643-016-0110-z ↗
75. Ramos-Romero, S., Torrella, J.R., Pagès, T., Viscor, G., and Torres, J.L. (2021). Edible Microalgae and Their Bioactive Compounds in the Prevention and Treatment of Metabolic Alterations. Nutrients, 13.10.3390/nu13020563 ↗
76. Andreeva, A., Budenkova, E., Babich, O., Sukhikh, S., Dolganyuk, V., Michaud, P., and Ivanova, S. (2021). Influence of Carbohydrate Additives on the Growth Rate of Microalgae Biomass with an Increased Carbohydrate Content. Mar. Drugs, 19.10.3390/md19070381 ↗
77. Chlorella for protein and biofuels: From strain selection to outdoor cultivation in a Green Wall Panel photobioreactor10.1186/1754-6834-7-84 ↗
78. Isolation and structural characterization of sulfated polysaccharide from Spirulina platensis and its bioactive potential: In vitro antioxidant, antibacterial activity and Zebrafish growth and reproductive performance10.1016/j.ijbiomac.2019.09.024 ↗
79. Microalgae polysaccharides bio-stimulating effect on tomato plants: Growth and metabolic distribution10.1016/j.btre.2020.e00426 ↗
80. Lactic acid bacteria growth promoters from Spirulina platensis10.1016/S0168-1605(98)00151-2 ↗
81. An overview: Biomolecules from microalgae for animal feed and aquaculture
82. Physico-chemical characteristics of the sulfated polysaccharides of the red microalgae Dixoniella grisea and Porphyridium aerugineum10.1016/j.ijbiomac.2019.09.205 ↗
83. Jacob-Lopes, E., Maroneze, M.M., Queiroz, M.I., and Zepka, L.Q. (2020). Chapter 18—Pigments from microalgae. Handbook of Microalgae-Based Processes and Products, Academic Press.10.1007/978-3-030-50971-2 ↗
84. Phototrophic pigment production with microalgae: Biological constraints and opportunities10.1111/jpy.12173 ↗
85. Production of astaxanthin from Haematococcus10.1016/0960-8524(91)90161-C ↗
86. Sathasivam, R., and Ki, J.-S. (2018). A Review of the Biological Activities of Microalgal Carotenoids and Their Potential Use in Healthcare and Cosmetic Industries. Mar. Drugs, 16.10.3390/md16010026 ↗
87. Microalgae Nannochloropsis oceanica as a future new natural source of vitamin D(3)10.1016/j.foodchem.2020.126627 ↗
88. Vitamin E (α-tocopherol) production by the marine microalgae Dunaliella tertiolecta and Tetraselmis suecica in batch cultivation10.1016/S1389-0344(03)00040-6 ↗
89. Characterization and bioavailability of vitamin B12-compounds from edible algae10.3177/jnsv.48.325 ↗
90. Characterization and Quantitation of Vitamin B12 Compounds in Various Chlorella Supplements10.1021/acs.jafc.6b03550 ↗
91. Chlorella and spirulina microalgae as sources of functional foods, nutraceuticals, and food supplements; an overview10.15406/mojfpt.2018.06.00144 ↗
92. Accumulation of antioxidant vitamins in Dunaliella salina
93. Microalgae as a source of vitamin K110.1016/j.algal.2018.10.008 ↗
94. Becker, W. (2003). Microalgae in Human and Animal Nutrition. Handbook of Microalgal Culture, John Wiley & Sons.10.1002/9780470995280.ch18 ↗
95. Growth and body conformation responses of genetically divergent Australian sheep to Spirulina (Arthrospira platensis) supplementation
96. Effect of various forms of dietary Chlorella supplementation on growth performance, immune characteristics, and intestinal microflora population of broiler chickens10.3382/japr.2012-00622 ↗
97. Yang, S.-T. (2007). Chapter 11—Production of High-Value Products by Marine Microalgae Thraustochytrids. Bioprocessing for Value-Added Products from Renewable Resources, Elsevier.
98. Effects of Haematococcus pluvialis supplementation on antioxidant system and metabolism in rainbow trout (Oncorhynchus mykiss)10.1007/s10695-011-9519-7 ↗
99. Microalga Isochrysis galbana in feed for Trachinotus ovatus: Effect on growth performance and fatty acid composition of fish fillet and liver10.1007/s10499-018-0282-y ↗
100. The effect of Nannochloropsis oceanica feed inclusion on rabbit muscle proteome10.1016/j.jprot.2020.103783 ↗