Application of Enzyme-Assisted Extraction for the Recovery of Natural Bioactive Compounds for Nutraceutical and Pharmaceutical Applications
Source abstract
Abstract
Enzyme-assisted extraction (EAE) involves the use of hydrolytic enzymes for the degradation of the cell wall or other cell components. This supports the diffusion of the solvent into the plant or fungal material, leading to easier elution of its metabolites. This technique has been gaining increasing attention, as it is considered an eco-friendly and cost-effective improvement on classical or modern extraction methods. Its promising application in improving the recovery of different classes of bioactive metabolites (e.g., polyphenols, carotenoids, polysaccharides, proteins, components of essential oil, and terpenes) has been reported by many scientific papers. This review summarises information on the theoretical aspects of EAE (e.g., the components of the cell walls and the types of enzymes used) and the most recent discoveries in the effective involvement of enzyme-assisted extraction of natural products (plants, mushrooms, and animals) for nutraceutical and pharmaceutical applications.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Microbial Metabolites in Food Biotechnology · Polysaccharides and Plant Cell Walls · Enzyme Catalysis and Immobilization
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Pharmaceutical Botany, Medical University of Lublin, 1 Chodźki Street, 20-093 Lublin, Poland
References (90)
Show source-provided references
1. The Role and Place of Medicinal Plants in the Strategies for Disease Prevention
2. Cosmetic ethnobotany practiced by tribal women of Kashmir Himalayas
3. Ethnobotanical survey of cosmetic plants used in Marquesas Islands (French Polynesia)10.1186/s13002-016-0128-5 ↗
4. Recent trends in extraction of plant bioactives using green technologies: A review10.1016/j.foodchem.2021.129431 ↗
5. Techniques for extraction and isolation of natural products: A comprehensive review10.1186/s13020-018-0177-x ↗
6. Optimization of ultrasonic-assisted enzymatic extraction of polysaccharides from thick-shell mussel (Mytilus coruscus) and their antioxidant activities10.1016/j.ijbiomac.2019.08.136 ↗
7. Environmentally Friendly Methods for Flavonoid Extraction from Plant Material: Impact of Their Operating Conditions on Yield and Antioxidant Properties
8. Carotenoid Metabolism in Plants: The Role of Plastids10.1016/j.molp.2017.09.010 ↗
9. The vacuolar transportome of plant specialized metabolites
10. Action of multi-enzyme complex on protein extraction to obtain a protein concentrate from okara10.1007/s13197-018-3067-4 ↗
11. Krakowska-Sieprawska, A., Rafińska, K., Walczak-Skierska, J., Kiełbasa, A., and Buszewski, B. (2021). Promising Green Technology in Obtaining Functional Plant Preparations: Combined Enzyme-Assisted Supercritical Fluid Extraction of Flavonoids Isolation from Medicago Sativa Leaves. Materials, 14.10.3390/ma14112724 ↗
12. Use of an enzyme-assisted method to improve protein extraction from olive leaves10.1016/j.foodchem.2014.07.116 ↗
13. Enzyme-aided release of bioactive compounds from coriander (Coriandrum sativum L.) seeds and their residue by-products and evaluation of their antioxidant activity10.1007/s13197-018-3229-4 ↗
14. Enzyme-Assisted Extraction of Triterpenoid Saponins from Pseuderanthemum palatiferum (Nees) Radlk. Dry Leaf Powder and Bioactivities Examination of Extracts10.1002/slct.201900778 ↗
15. Improving the extraction efficiency and stability of β-carotene from carrot by enzyme-assisted green nanoemulsification10.1016/j.ifset.2021.102836 ↗
16. Enzyme-assisted extraction of bioactive compounds from ginger (Zingiber officinale Roscoe)10.1016/j.foodchem.2013.01.099 ↗
17. Recovery of Biomolecules from Agroindustry by Solid-Liquid Enzyme-Assisted Extraction: A Review10.1007/s12161-021-01974-w ↗
18. The plant cell wall: Biosynthesis, construction, and functions10.1111/jipb.13055 ↗
19. Plant cell walls10.1016/j.cub.2017.05.025 ↗
20. The plant cell wall: A complex and dynamic structure as revealed by the responses of genes under stress conditions10.3389/fpls.2016.00984 ↗
21. Plant cell wall composition and enzymatic deconstruction10.3934/bioeng.2018.1.63 ↗
22. The influence of lignin content and structure on hemicellulose alkaline extraction for non-wood and hardwood lignocellulosic biomass10.1007/s10570-019-02261-y ↗
23. The Fungal Cell Wall: Structure, Biosynthesis, and Function10.1128/microbiolspec.FUNK-0035-2016 ↗
24. Free, S.J. (2013). Fungal Cell Wall Organization and Biosynthesis. Advances in Genetics, Elsevier.10.1016/B978-0-12-407677-8.00002-6 ↗
25. Ibe, C., and Munro, C.A. (2021). Fungal cell wall proteins and signaling pathways form a cytoprotective network to combat stresses. J. Fungi, 7.10.3390/jof7090739 ↗
26. Enzymatically assisted extraction of antioxidant and anti-mutagenic compounds from radish (Raphanus sativus)10.1016/j.eti.2021.101620 ↗
27. Sequential extraction of Hericium erinaceus using green solvents10.1016/j.lwt.2015.06.008 ↗
28. Celulazy-właściwości, otrzymywanie i zastosowanie (Cellulases-properties, application and production)
29. Enhanced production of cellobiase by marine bacterium Cellulophaga lytica LBH-14 from rice bran under optimized conditions involved in dissolved oxygen10.1007/s12257-014-0486-6 ↗
30. Cellulases, Hemicellulases, and Pectinases: Applications in the Food and Beverage Industry10.1007/s11947-021-02678-z ↗
31. Characterization and influence of a multi-enzymatic biopreparation for biogas yield enhancement10.15376/biores.12.3.6187-6206 ↗
32. Production and characterization of cellulase by Bacillus pumilus EB3
33. Characterization of the Cellulase Complex of Penicillium echinulatum10.1080/10242420400024532 ↗
34. Purification and characterization of a protease-resistant cellulase from Aspergillus niger10.1016/0922-338X(95)94078-6 ↗
35. Isolation, purification and characterization of cellulase produced by Aspergillus niger cultured on Arachis hypogaea shells10.1016/j.heliyon.2020.e05668 ↗
36. Ejaz, U., Sohail, M., and Ghanemi, A. (2021). Cellulases: From Bioactivity to a Variety of Industrial Applications. Biomimetics, 6.10.3390/biomimetics6030044 ↗
37. Pectin and Pectinases: Production, Characterization and Industrial Application of Microbial Pectinolytic Enzymes10.2174/1874070700903010009 ↗
38. Production and characterization of thermostable xylanase and pectinase from Streptomyces sp. QG-11-310.1038/sj.jim.7000010 ↗
39. Production and characterization of pectinase enzyme from Penicillium chrysogenum10.17485/ijst/2010/v3i4.10 ↗
40. Production, purification and characterization of an exo-polygalacturonase from Penicillium janthinellum sw0910.1590/0001-3765201620150051 ↗
41. Biochemical characterization of three distinct polygalacturonases from Neosartorya fischeri P110.1016/j.foodchem.2015.05.022 ↗
42. Hemicelulazy-właściwości, otrzymywanie i zastosowanie
43. A novel family of hemicellulolytic alpha-glucuronidase10.1016/j.febslet.2009.03.057 ↗
44. Characterization of an acetyl xylan esterase from the marine bacterium Ochrovirga pacifica and its synergism with xylanase on beechwood xylan10.1186/s12934-019-1169-y ↗
45. Thomas, L., Joseph, A., Singhania, R.R., Patel, A.K., and Pandey, A. (2016). Industrial Enzymes: Xylanases. Current Developments in Biotechnology and Bioengineering: Production, Isolation and Purification of Industrial Products, Elsevier.10.1016/B978-0-444-63662-1.00006-3 ↗
46. Xylanases from fungi: Properties and industrial applications10.1007/s00253-005-1904-7 ↗
47. Bacterial and Fungal Proteolytic Enzymes: Production, Catalysis and Potential Applications10.1007/s12010-017-2427-2 ↗
48. Kieliszek, M., Pobiega, K., Piwowarek, K., and Kot, A.M. (2021). Characteristics of the proteolytic enzymes produced by lactic acid bacteria. Molecules, 26.10.3390/molecules26071858 ↗
49. Production of plant proteases in vivo and in vitro—A review10.1016/j.biotechadv.2011.08.017 ↗
50. Optimization of enzyme assisted extraction of polysaccharides from Tricholoma matsutake by response surface methodology10.1016/j.carbpol.2011.06.053 ↗
51. Surfactant-assisted enzymatic extraction of piperine from Piper nigrum L10.1080/10942912.2019.1707221 ↗
52. Microwave-assisted enzymatic extraction of plant protein with antioxidant compounds from the food waste sesame bran: Comparative optimization study and identification of metabolomics using LC/Q-TOF/MS
53. Enzyme-assisted extraction of astaxanthin from Haematococcus pluvialis and its stability and antioxidant activity10.1007/s10068-019-00608-6 ↗
54. Advantageous enzyme selective extraction process of essential spirulina oil10.1016/j.cattod.2019.02.066 ↗
55. Astaxanthin recovery from Atlantic shrimp (Pandalus borealis) processing materials10.1016/j.biteb.2020.100535 ↗
56. Enzymatic extraction and characterization of polysaccharide from Tuber aestivum10.1016/j.bcdf.2017.02.001 ↗
57. Characterization and Optimization of Process Parameters for Enzyme Assisted Extraction of Kendu (Diospyros Melanoxylon Roxb.) Fruit Juice10.1080/15538362.2021.1873220 ↗
58. Multi-objective optimization of enzyme-assisted juice extraction from custard apple: An integrated approach using RSM and ANN coupled with sensory acceptance10.1111/jfpp.15256 ↗
59. Aqueous enzymatic extraction and demulsification of camellia seed oil (Camellia oleifera Abel.) and the oil’s physicochemical properties10.1002/ejlt.201400582 ↗
60. Enzyme-assisted microwave hydro-distillation essential oil from Fructus forsythia, chemical constituents, and its antimicrobial and antioxidant activities10.1016/j.foodchem.2012.02.114 ↗
61. Optimization of ultrasound-assisted enzymatic extraction of walnut kernel oil using response surface methodology10.1111/jfpe.12696 ↗
62. Enzyme-assisted extraction of glycyrrhizic acid from licorice roots using heat reflux and ultrasound methods10.1016/j.bcab.2021.101953 ↗
63. Optimisation of the enzyme-assisted extraction of polyphenols from saffron (Crocus sativus L.) tepals
64. Pontillo, A.R.N., Papakosta-Tsigkri, L., Lymperopoulou, T., Mamma, D., Kekos, D., and Detsi, A. (2021). Conventional and Enzyme-Assisted Extraction of Rosemary Leaves (Rosmarinus officinalis L.): Toward a Greener Approach to High Added-Value Extracts. Appl. Sci., 11.10.3390/app11083724 ↗
65. Enzyme-assisted extraction of carotenoids and phenolic compounds from sunflower wastes using green solvents10.1007/s13205-020-02393-0 ↗
66. Enzyme-assisted three-phase partitioning: An efficient alternative for oil extraction from sesame (Sesamum indicum L.)10.3989/gya.1060182 ↗
67. Enzyme-Assisted Extraction of Bioactive Compounds from Raspberry (Rubus idaeus L.) Pomace10.1111/1750-3841.14625 ↗
68. Enzymatic extraction optimization, anti-HBV and antioxidant activities of polysaccharides from Viscum coloratum (Kom.) Nakai10.1016/j.ijbiomac.2019.04.173 ↗
69. Optimization of ultrasound-assisted enzymatic extraction and antioxidant activity of polysaccharide from radix Morindae officinalis by response surface methodology10.4103/pm.pm_444_19 ↗
70. Optimisation of novel method for the extraction of steviosides from Stevia rebaudiana leaves10.1016/j.foodchem.2011.11.063 ↗
71. Optimization of enzyme assisted extraction of lycopene from industrial tomato waste10.1016/j.seppur.2019.03.006 ↗
72. Lombardelli, C., Benucci, I., Mazzocchi, C., and Esti, M. (2021). A novel process for the recovery of betalains from unsold red beets by low-temperature enzyme-assisted extraction. Foods, 10.10.3390/foods10020236 ↗
73. Physical and Chemical Influences of Different Extraction Techniques for Essential Oil Recovery from Citrus sinensis Peels10.1080/0972060X.2021.1925596 ↗
74. Optimization of microwave-assisted enzymatic extraction of polysaccharides from the fruit of Schisandra chinensis Baill10.1016/j.ijbiomac.2015.01.048 ↗
75. Inhibition of cellulases by phenols10.1016/j.enzmictec.2009.11.001 ↗
76. Different Inhibitors of the Extracellular Pectinolytic Enzymes of Fusarium oxysporum and Rhizoctonia solani
77. Changes in Essential Oil during Enzyme-Assisted Ensiling of Lemongrass (Cymbopogon citratus Stapf.) and Lemon Eucalyptus (Eucalyptus citriodora Hook)10.1021/jf001324+ ↗
78. Comparison of enzyme-assisted and ultrasound-assisted extraction of vitamin C and phenolic compounds from acerola (Malpighia emarginata DC.) fruit10.1111/j.1365-2621.2012.02960.x ↗
79. Recovery of high-value bioactive phytochemicals from agro-waste of mango (Mangifera indica L.) using enzyme-assisted ultrasound pretreated extraction
80. Evaluation of Cellulolytic Enzyme-Assisted Microwave Extraction of Punica granatum Peel Phenolics and Antioxidant Activity10.1007/s11130-020-00859-3 ↗
81. Zhang, M., Ma, W., Wang, C., Yang, X., Lou, Y., Xia, X., and Xu, H. (2021). Optimization of Enzyme-Assisted Extraction and Purification of Flavonoids from Pinus koraiensis Nut-Coated Film and Antioxidant Activity Evaluation. Molecules, 26.10.3390/molecules26071950 ↗
82. Application of enzyme-assisted extraction of baicalin from Scutellaria baicalensis Georgi10.1080/10826068.2020.1808791 ↗
83. Cocktail enzyme-assisted alkaline extraction and identification of jujube peel pigments10.1016/j.foodchem.2021.129747 ↗
84. The usage of enzyme in ultrasound-assisted enzymatic extraction method and its effect on yield extract from Keji Beling (Strobilanthes crispus.) leaves10.1051/e3sconf/20186703002 ↗
85. Antioxidant pectin enriched fractions obtained from discarded carrots (Daucus carota L.) by ultrasound-enzyme assisted extraction10.1016/j.foodchem.2019.03.078 ↗
86. Extraction, Characterization, Antioxidant, and Immunostimulatory Activities of Polysaccharides from Hedyotis corymbosa10.1155/2018/8617070 ↗
87. Response surface optimization of ultrasound-assisted enzymatic extraction polysaccharides from Lycium barbarum10.1016/j.carbpol.2014.03.040 ↗
88. Optimization of the microwave-assisted enzymatic extraction of Rosa roxburghii Tratt. polysaccharides using response surface methodology and its antioxidant and α-d-glucosidase inhibitory activity10.1016/j.ijbiomac.2018.02.003 ↗
89. Kinetic model and optimization for enzyme-assisted hydrodistillation of d-limonene-rich essential oil from orange peel10.1002/ffj.3598 ↗
90. Application of surface response methodology for an enzyme-assisted extraction of carotenoids10.15414/jmbfs.2019.9.2.195-203 ↗