journal article·2017-05-02Open access

Automated Diatom Classification (Part B): A Deep Learning Approach

Anibal Pedraza, Gloria Bueno, Oscar Deniz, Gabriel Cristóbal, Saúl Blanco, María Borrego-Ramos

Applied Sciences· Volume 7· Issue 5

Abstract

Diatoms, a kind of algae microorganisms with several species, are quite useful for water quality determination, one of the hottest topics in applied biology nowadays. At the same time, deep learning and convolutional neural networks (CNN) are becoming an extensively used technique for image classification in a variety of problems. This paper approaches diatom classification with this technique, in order to demonstrate whether it is suitable for solving the classification problem. An extensive dataset was specifically collected (80 types, 100 samples/type) for this study. The dataset covers different illumination conditions and it was computationally augmented to more than 160,000 samples. After that, CNNs were applied over datasets pre-processed with different image processing techniques. An overall accuracy of 99% is obtained for the 80-class problem and different kinds of images (brightfield, normalized). Results were compared to previous presented classification techniques with different number of samples. As far as the authors know, this is the first time that CNNs are applied to diatom classification.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Identification and Quantification in Food · Diatoms and Algae Research · Digital Imaging for Blood Diseases

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

VISILAB Research Group, University of Castilla La Mancha, Av. Camilo José Cela s/n, Ciudad Real 13071, Spain

Institute of Optics, Spanish National Research Council (CSIC), Serrano 121, Madrid 8006, Spain

The Institute of the Environment, University of Leon, León E-24071, Spain

References (14)

Show source-provided references
1. The European Parliament and the Council of the European Union (2000). Directive 2000/60/EC. Establishing a Framework for Community Action in the Field of Water Policy, Official Journal of the European Community.
2. Are biotic indices sensitive to river toxicants? A comparison of metrics based on diatoms and macro-invertebrates10.1016/j.chemosphere.2010.01.059 ↗
3. Smol, J., and Stoermer, E. (2010). The Diatoms: Applications for the Environmental and Earth Sciences, Cambridge University Press.10.1017/CBO9780511763175 ↗
4. Bueno, G., Deniz, O., Pedraza, A., Salido, J., Cristobal, G., and Saul, B. (2017). Automated Diatom Classification (Part A): Handcrafted feature approaches. Appl. Sci., in press.10.3390/app7080753 ↗
5. Hierarchical classification of diatom images using ensembles of predictive clustering trees10.1016/j.ecoinf.2011.09.001 ↗
6. Krizhevsky, A., Sutskever, I., and Hinton, G. (2012). Imagenet classification with deep convolutional neural networks. Advances in Neural Information Processing Systems, Neural Information Processing Systems Foundation, Inc.
7. Szegedy, C., Liu, W., Jia, Y., Sermanet, P., Reed, S., Anguelov, D., Erhan, D., Vanhoucke, V., and Rabinovich, A. (2015, January 7–12). Going deeper with convolutions. Proceedings of the IEEE Conference on Computer Vision and Pattern Recognition, Boston, MA, USA.10.1109/CVPR.2015.7298594 ↗
8. Du Buf, H., and Bayer, M. (2002). Automatic Diatom Identification, World Scientific Publishing Co.10.1142/4907 ↗
9. Legendre shape descriptors and shape group determination of specimens in the Cymbella cistula species complex10.2216/i0031-8884-42-1-90.1 ↗
10. High-throughput time-stretch imaging flow cytometry for multi-class classification of phytoplankton10.1364/OE.24.028170 ↗
11. Gonzalez, R., and Woods, R. (2008). Digital Image Processing, Pearson Prentice Hall.
12. Adaptive subgradient methods for online learning and stochastic optimization
13. Nesterov, Y. (2007). Gradient Methods for Minimizing Composite Objective Function, University College London. Technical Report.
14. An introduction to ROC analysis10.1016/j.patrec.2005.10.010 ↗