journal article·2026-08

Bridging biology and technology: In vitro 3D liver models for drug metabolism and pharmacokinetic studies

Haoyue Xu, Chengheng Dai, Shiyi Sun, Zhiyu Liu, Zhiwei Hu, Changmin Shao, Ziming Wang

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OpenAlex topics: 3D Printing in Biomedical Research · Pharmacogenetics and Drug Metabolism · Liver physiology and pathology

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References (100+)

Show source-provided references
1. In vitro models replicating the human intestinal epithelium for absorption and metabolism studies: A systematic review10.1016/j.jconrel.2021.05.028 ↗
2. Recent advances in the translation of drug metabolism and pharmacokinetics science for drug discovery and development10.1016/j.apsb.2022.03.009 ↗
3. Altered peripheral factors affecting the absorption, distribution, metabolism, and excretion of oral medicines in Alzheimer’s disease10.1016/j.addr.2022.114282 ↗
4. Unveiling gut microbiota's role: bidirectional regulation of drug transport for improved safety10.1002/med.22077 ↗
5. Advanced application of stimuli-responsive drug delivery system for inflammatory arthritis treatment
6. Design and measurement of drug tissue concentration asymmetry and tissue exposure-effect (Tissue PK-PD) evaluation10.1021/acs.jmedchem.2c00502 ↗
7. Bioengineered silk protein-based 3D in vitro models for tissue engineering and drug development: from silk matrix properties to biomedical applications10.1002/adhm.202401458 ↗
8. FDA Modernization Act 2.0: transitioning beyond animal models with human cells, organoids, and AI/ML-based approaches10.1172/JCI175824 ↗
9. Limitations of animal studies for predicting toxicity in clinical trials10.1016/j.jacbts.2020.03.010 ↗
10. Current advances in 3D dynamic cell culture systems10.3390/gels8120829 ↗
11. Emerging trends in the methodology of environmental toxicology: 3D cell culture and its applications10.1016/j.scitotenv.2022.159501 ↗
12. Three-dimensional bio-printing of primary human hepatocellular carcinoma for personalized medicine
13. A review on new frontiers in drug-drug interaction predictions and safety evaluations with in vitro cellular models10.3390/pharmaceutics17060747 ↗
14. Expansion of human primary hepatocytes in vitro through their amplification as liver progenitors in a 3D organoid system10.1038/s41598-018-26584-1 ↗
15. 3D in vitro model of differentiated HepG2 cell spheroids with improved liver-like properties for repeated dose high-throughput toxicity studies
16. Characterization of primary human hepatocyte spheroids as a model system for drug-induced liver injury, liver function and disease10.1038/srep25187 ↗
17. Multi-cell type human liver microtissues for hepatotoxicity testing10.1007/s00204-012-0968-2 ↗
18. Utility of spherical human liver microtissues for prediction of clinical drug-induced liver injury10.1007/s00204-017-2002-1 ↗
19. Comparison of hepatic 2D sandwich cultures and 3D spheroids for long-term toxicity applications: a multicenter study10.1093/toxsci/kfx289 ↗
20. Generation of human adult hepatocyte organoids with metabolic functions10.1038/s41586-025-08861-y ↗
21. Integration of in vitro data from three dimensionally cultured HepaRG cells and physiologically based pharmacokinetic modeling for assessment of acetaminophen hepatotoxicity10.1016/j.yrtph.2020.104661 ↗
22. Microscale collagen and fibroblast interactions enhance primary human hepatocyte functions in three-dimensional models10.3727/105221620X15868728381608 ↗
23. Self-assembled matrigel-free iPSC-derived liver organoids demonstrate wide-ranging highly differentiated liver functions10.1093/stmcls/sxac090 ↗
24. Precision-cut liver slices: a versatile tool to advance liver research10.1007/s12072-018-9913-7 ↗
25. 3D liver model for assessing immune-mediated hepatotoxicity from biopharmaceutical risks10.1016/j.cej.2025.168419 ↗
26. Advanced liver-on-a-chip model for evaluating drug metabolism and hepatotoxicity10.3390/bios14090435 ↗
27. A 3D spheroid model of quadruple cell co-culture with improved liver functions for hepatotoxicity prediction10.1016/j.tox.2024.153829 ↗
28. Impact of culture medium on cellular interactions in in vitro co-culture systems10.3389/fbioe.2020.00911 ↗
29. Comparative analysis of 3D-culture techniques for multicellular colorectal tumour spheroids and development of a novel SW48 3D-model10.1038/s41598-025-13588-x ↗
30. Drugs and the liver10.1016/j.mpaic.2020.07.001 ↗
31. In vitro three-dimensional (3D) cell culture tools for spheroid and organoid models10.1016/j.slasd.2023.03.006 ↗
32. From the cover: three-dimensional (3D) HepaRG spheroid model with physiologically relevant xenobiotic metabolism competence and hepatocyte functionality for liver toxicity screening10.1093/toxsci/kfx122 ↗
33. Recent advances of integrated microfluidic suspension cell culture system10.1049/enb2.12015 ↗
34. Microfluidic based single cell or droplet manipulation: methods and applications10.1016/j.talanta.2023.124776 ↗
35. Current research trends in the application of in vitro three-dimensional models of liver cells10.3390/pharmaceutics15010054 ↗
36. A comprehensive review of advances in hepatocyte microencapsulation: Selecting materials and preserving cell viability
37. In situ self-assembling liver spheroids with synthetic nanoscaffolds for preclinical drug screening application10.1021/acsami.3c17384 ↗
38. Gaining new biological and therapeutic applications into the liver with 3D in vitro liver models10.1007/s13770-020-00245-9 ↗
39. Magnetic-based human tissue 3D cell culture: a systematic review10.3390/ijms232012681 ↗
40. Mechanical properties of cell sheets and spheroids: the link between single cells and complex tissues10.1007/s12551-021-00821-w ↗
41. Magnetic nanoparticles behavior in biological solutions; the impact of clustering tendency on sedimentation velocity and cell uptake10.3390/ma13071644 ↗
42. Applications and sensory utilizations of magnetic levitation in 3D cell culture for tissue Engineering10.1007/s11033-023-08585-0 ↗
43. Three-dimensional (3D) liver cell models-a tool for bridging the gap between animal studies and clinical trials when screening liver accumulation and toxicity of nanobiomaterials10.1007/s13346-022-01147-0 ↗
44. Advances in the application of extracellular vesicles derived from three-dimensional culture of stem cells10.1186/s12951-024-02455-y ↗
45. 3D culture of fibroblasts and neuronal cells on microfabricated free-floating carriers10.1016/j.colsurfb.2023.113350 ↗
46. Perfusion of 3D encapsulated hepatocytes-a synergistic effect enhancing long-term functionality in bioreactors10.1002/bit.22920 ↗
47. Three-dimensional liver culture systems to maintain primary hepatic properties for toxicological analysis in vitro10.3390/ijms221910214 ↗
48. 3D cell culture systems: tumor application, advantages, and disadvantages10.3390/ijms222212200 ↗
49. Biomimetic scaffolds-a novel approach to three dimensional cell culture techniques for potential implementation in tissue engineering10.3390/nano14060531 ↗
50. Applications of biocompatible scaffold materials in stem cell-based cartilage tissue engineering10.3389/fbioe.2021.603444 ↗
51. 3D culture of HepaRG cells in GelMa and its application to bioprinting of a multicellular hepatic model10.1016/j.biomaterials.2020.120611 ↗
52. A review of preparation methods of porous skin tissue engineering scaffolds
53. Review on porous scaffolds generation process: a tissue engineering approach10.1021/acsabm.2c00740 ↗
54. The effect of pore size and layout on mechanical and biological properties of 3D-printed bone scaffolds with gradient porosity10.1002/pc.27174 ↗
55. Long-term liver-specific functions of hepatocytes in electrospun chitosan nanofiber scaffolds coated with fibronectin10.1002/jbm.a.36072 ↗
56. Fabrication and evaluation of modified poly (ethylene terephthalate) microfibrous scaffolds for hepatocyte growth and functionality maintenance10.1016/j.msec.2019.110523 ↗
57. Advancements in the use of hydrogels for regenerative medicine: properties and biomedical applications10.1155/2022/3606765 ↗
58. A review on the design and hydration properties of natural polymer-based hydrogels10.3390/ma14051095 ↗
59. Advances in biodegradable and injectable hydrogels for biomedical applications10.1016/j.jconrel.2020.12.008 ↗
60. Hydrogels: properties and applications in biomedicine10.3390/molecules27092902 ↗
61. Stiffening hydrogels for investigating the dynamics of hepatic stellate cell mechanotransduction during myofibroblast activation10.1038/srep21387 ↗
62. High-throughput production of liver parenchymal microtissues and enrichment of organ-specific functions in gelatin methacrylamide microenvironment10.1002/bit.28010 ↗
63. Nanofibrillar cellulose hydrogel promotes three-dimensional liver cell culture10.1016/j.jconrel.2012.06.039 ↗
64. Upgrading hepatic differentiation and functions on 3D printed silk-decellularized liver hybrid scaffolds10.1021/acsbiomaterials.1c00671 ↗
65. Biofabrication of hepatic constructs by 3D bioprinting of a cell-laden thermogel: an effective tool to assess drug-induced hepatotoxic response
66. Photocrosslinkable liver extracellular matrix hydrogels for the generation of 3D liver microenvironment models10.1038/s41598-021-94990-z ↗
67. Viability, function and morphological integrity of precision-cut liver slices during prolonged incubation: effects of culture medium10.1016/j.tiv.2015.10.008 ↗
68. Liver slice culture as a model for lipid metabolism in fish10.7717/peerj.7732 ↗
69. Generation of a high‐precision whole liver panorama and cross‐scale 3D pathological analysis for hepatic fibrosis
70. Microfluidic programmable strategies for channels and flow10.1039/D4LC00423J ↗
71. Microfluidic 3D hepatic cultures integrated with a droplet-based bioanalysis unit10.1016/j.bios.2023.115896 ↗
72. Microfluidic fabrication of core-shell microcapsules carrying human pluripotent stem cell spheroids
73. Development and future of droplet microfluidics10.1039/D3LC00729D ↗
74. DNA-templated assembly of droplet-derived PEG microtissues10.1039/c1lc20318e ↗
75. Recent methods of droplet microfluidics and their applications in spheroids and organoids10.1039/D2LC00493C ↗
76. Digital microfluidics for drug metabolism research in droplet-scale
77. A high-throughput microfluidic platform for functional hiPSC-derived liver organoids with bile duct-and lobule-like structures10.1039/D5LC00154D ↗
78. Cell culture in microfluidic droplets10.1021/acs.chemrev.1c00666 ↗
79. Droplet microfluidics for advanced single-cell analysis10.1002/smmd.70002 ↗
80. Hydrogel-based strategies for liver tissue engineering
81. Human ESC-derived expandable hepatic organoids enable therapeutic liver repopulation and pathophysiological modeling of alcoholic liver injury10.1038/s41422-019-0242-8 ↗
82. Modeling hepatic steatosis with human adult stem cell-derived liver organoids
83. Liver organoids: formation strategies and biomedical applications10.1007/s13770-021-00357-w ↗
84. Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation10.1038/nprot.2016.097 ↗
85. Generation of functional liver organoids on combining hepatocytes and cholangiocytes with hepatobiliary connections ex vivo10.1038/s41467-021-23575-1 ↗
86. Developing organs-on-chips for biomedical applications10.1002/SMMD.20240009 ↗
87. organs-on-chips: into the next decade10.1038/s41573-020-0079-3 ↗
88. Advanced liver-on-chip model mimicking hepatic lobule with continuous microvascular network via high-definition laser patterning
89. Modeling of lung-liver interaction during infection in a human microfluidic organ-on-a-chip10.1038/s41598-025-22682-z ↗
90. Organs-on-a-chip: a fast track for engineered human tissues in drug development10.1016/j.stem.2018.02.011 ↗
91. In vitro grafting of hepatic spheroids and organoids on a microfluidic vascular bed10.1007/s10456-022-09842-9 ↗
92. A perfusion incubator liver chip for 3D cell culture with application on chronic hepatotoxicity testing10.1038/s41598-017-13848-5 ↗
93. Patient-specific organoid and organ-on-a-chip: 3D cell-culture meets 3D printing and numerical simulation
94. From organoids to organoids-on-a-chip: current applications and challenges in biomedical research10.1097/CM9.0000000000003535 ↗
95. Hepatic sinusoids versus central veins: structures, markers, angiocrines, and roles in liver regeneration and homeostasis10.1002/ar.24560 ↗
96. Role of liver sinusoidal endothelial cells in liver diseases10.1038/s41575-020-00411-3 ↗
97. Mimicking liver sinusoidal structures and functions using a 3D-configured microfluidic chip10.1039/C6LC01374K ↗
98. Liver-on-a-chip models of fatty liver disease10.1002/hep.31106 ↗
99. Multi-organ-on-a-chip for pharmacokinetics and toxicokinetic study of drugs10.1080/17425255.2021.1908996 ↗
100. Acetaminophen absorption and metabolism in an intestine/liver microphysiological system10.1016/j.cbi.2018.11.010 ↗