Polypeptide coatings on biominerals with superior antimicrobial and antifouling properties inspired by human salivary proteins
Source abstract
Abstract
Inhibiting the growth of bacteria on biomedical implants is of great clinical significance due to the associated life-threatening infections. Peptide sequence DDDEEK (SAP) and NFKRKYGHHRKA (P1) are inspired from the salivary proteins statherin and histatin, respectively. SAP enables stable immobilization on the biomineral surface, and P1 has a strong antimicrobial effect against clinically pathogenic bacteria. Herein, we report the surface-immobilized multifunctional coating based on a hybrid peptide NFKRKYGHHRKAKEEDDD (P1-SAP). This coating can form on hydroxyapatite (HA)/tricalcium phosphate (TCP) surfaces by a simple dip-coating or spraying method and remain on the surface for at least 1 month. The P1-SAP coating exhibits 3-fold effective antimicrobial properties against bacteria/biofilm and shows 2-fold antifouling functions than the bare group due to the synergistic effect of superhydrophilic surface, cationic peptides and surface hydrophobic groups. Additionally, the combination of SAP with P1 can reduce the cytotoxicity of P1 to MG63 cells because the negative charges of SAP can effectively neutralize the positive charges of P1. Moreover, in vivo studies in rats show a significant decrease in infection associated with the P1-SAP-coated HA implants, indicating the efficacy of this coating against subcutaneous infections. Our findings provide valuable guidance to the design and evaluation of coatings with antimicrobial and antifouling properties for implant surfaces.
Source: expertdata · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Antimicrobial Peptides and Activities · Silk-based biomaterials and applications · Oral microbiology and periodontitis research
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
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