Oligoglycine and fluoropolymer functionalized enzyme-responsive gene delivery surface for rapid in situ endothelialization of vascular grafts
Source abstract
Abstract
The treatment success of small-caliber artificial vascular grafts is seriously limited by high thrombogenesis and low endothelialization. Gene transfection can effectively enhance the proliferation and migration of endothelial cells (ECs) and accelerating the formation of endothelium layer. How to efficiently and temporarily immobilize the gene complexes on the material surface, how to intelligently release them and effectively transfect ECs are still the challenges for in situ endothelialization. Herein, a matrix-metalloproteinase (MMP)-responsive gene delivery system is constructed for in situ smart release of genes from the graft surface. The fluorinated cationic polymer (F-ssPEI) is served as high-efficient and low-cytotoxic gene vector. The gene complexes are temporarily immobilized via a MMP-cleavable peptide (MCP) linker which contains a MMP-susceptible sequence as the principal part and oligoglycine sequence (Gm) with different units (m = 0, 4 and 8) as the flexible spacer arm. These gene delivery surfaces achieve the temporary immobilization, smart release and high internalization of gene complexes. The MCP(G8) group shows the highest transfection efficacy and the optimal HUVEC proliferation/migration/function. The enhanced endothelialization and contractile smooth muscle cell regeneration of grafts are achieved in vivo. The MMP-responsive gene delivery system provides an effective platform to promote regeneration of vascular graft.
Source: expertdata · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: RNA Interference and Gene Delivery · Electrospun Nanofibers in Biomedical Applications · Advanced biosensing and bioanalysis techniques
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (70)
Show source-provided references
1. Surface modification and endothelialization of biomaterials as potential scaffolds for vascular tissue engineering applications10.1039/C4CS00483C ↗
2. Surface engineering of cardiovascular devices for improved hemocompatibility and rapid endothelialization10.1002/adhm.202000920 ↗
3. Heart disease and stroke statistics—2019 update: a report from the American heart association10.1161/CIR.0000000000000659 ↗
4. Medical device-induced thrombosis: what causes it and how can we prevent it?10.1111/jth.12961 ↗
5. Surface modification of implanted cardiovascular metal stents: from antithrombosis and antirestenosis to endothelialization10.1002/jbm.a.34714 ↗
6. Endothelialization of cardiovascular devices10.1016/j.actbio.2019.08.042 ↗
7. CREDVW-linked polymeric micelles as a targeting gene transfer vector for selective transfection and proliferation of endothelial cells10.1021/acsami.5b02399 ↗
8. Nanoparticles complexed with gene vectors to promote proliferation of human vascular endothelial cells10.1002/adhm.201400817 ↗
9. Core/shell gene carriers with different lengths of PLGA chains to transfect endothelial cells10.1021/acs.langmuir.7b02934 ↗
10. Novel zinc finger transcription factor ZFP580 promotes differentiation of bone marrow-derived endothelial progenitor cells into endothelial cells via eNOS/NO pathway10.1016/j.yjmcc.2015.08.004 ↗
11. ZNF580 mediates eNOS expression and endothelial cell migration/proliferation via the TGF-beta1/ALK5/Smad2 pathway10.1007/s11010-014-2061-z ↗
12. From single to a dual-gene delivery nanosystem: coordinated expression matters for boosting the neovascularization in vivo10.1039/C9BM02000D ↗
13. CAGW peptide- and PEG-modified gene carrier for selective gene delivery and promotion of angiogenesis in HUVECs in vivo10.1021/acsami.6b14769 ↗
14. Multifunctional peptide conjugated amphiphilic cationic copolymer for enhancing ECs targeting, penetrating and nuclear accumulation10.1007/s11705-020-1919-8 ↗
15. Redox stimulus disulfide conjugated polyethyleneimine as a shuttle for gene transfer10.1007/s10856-020-06457-8 ↗
16. A fluorinated dendrimer achieves excellent gene transfection efficacy at extremely low nitrogen to phosphorus ratios10.1038/ncomms4053 ↗
17. Evaluation of the hydrophobicity of perfluoroalkyl chains in amphiphilic compounds that are incorporated into cell membrane10.1016/j.jfluchem.2011.01.004 ↗
18. Self-assembled fluorodendrimers combine the features of lipid and polymeric vectors in gene delivery10.1002/anie.201501461 ↗
19. High DNA-binding affinity and gene-transfection efficacy of bioreducible cationic nanomicelles with a fluorinated core10.1002/ange.201508695 ↗
20. Surface-engineered dendrimers in gene delivery10.1021/cr500542t ↗
21. The effect of fluorination on the transfection efficacy of surface-engineered dendrimers10.1016/j.biomaterials.2014.04.065 ↗
22. PEI fluorination reduces toxicity and promotes liver-targeted siRNA delivery10.1007/s13346-020-00790-9 ↗
23. Low molecular weight PEI-based fluorinated polymers for efficient gene delivery10.1016/j.ejmech.2018.11.041 ↗
24. ROS-responsive fluorinated polyethyleneimine vector to co-deliver shMTHFD2 and shGPX4 plasmids induces ferroptosis and apoptosis for cancer therapy10.1016/j.actbio.2021.11.042 ↗
25. Surface-mediated stimuli-responsive gene delivery based on breath figure film combined with matrix metalloproteinase-sensitive hydrogel10.1021/acsbiomaterials.9b01353 ↗
26. Enzyme-responsive nanoparticles for targeted accumulation and prolonged retention in heart tissue after myocardial infarction10.1002/adma.201502003 ↗
27. Bioresponsive materials10.1038/natrevmats.2016.75 ↗
28. MMP9-sensitive polymers mediate environmentally-responsive bivalirudin release and thrombin inhibition10.1039/C4BM00259H ↗
29. Gellan gum hydrogels with enzyme-sensitive biodegradation and endothelial cell biorecognition sites10.1002/adhm.201700686 ↗
30. Matrix-metalloproteinase-responsive gene delivery surface for enhanced in situ endothelialization10.1021/acsami.0c11971 ↗
31. Endothelial cell-mediated gene delivery for in situ accelerated endothelialization of a vascular graft10.1021/acsami.1c01869 ↗
32. A peptide-network weaved nanoplatform with tumor microenvironment responsiveness and deep tissue penetration capability for cancer therapy10.1002/adma.201501502 ↗
33. Immobilization of heparin on the surface of polypropylene non-woven fabric for improvement of the hydrophilicity and blood compatibility10.1163/156856211X621088 ↗
34. Hydrophilic PCU scaffolds prepared by grafting PEGMA and immobilizing gelatin to enhance cell adhesion and proliferation10.1016/j.msec.2015.02.015 ↗
35. Surface modification with PEG and hirudin for protein resistance and thrombin neutralization in blood contact10.1016/j.colsurfb.2010.07.024 ↗
36. Construction of hemocompatible and histocompatible surface by grafting antithrombotic peptide ACH11 and hydrophilic PEG10.1021/acsbiomaterials.9b00431 ↗
37. Multifunctional gene carriers with enhanced specific penetration and nucleus accumulation to promote neovascularization of HUVECs in vivo10.1021/acsami.7b11615 ↗
38. Identification and characterization of receptor-specific peptides for siRNA delivery10.1021/nn301975s ↗
39. The spacer arm length in cell-penetrating peptides influences chitosan/siRNA nanoparticle delivery for pulmonary inflammation treatment10.1039/C5NR06903C ↗
40. Investigation of the influence of spacer arm on the structural evolution of affinity ligands supported on agarose10.1021/jp0622278 ↗
41. Effect of spacer arm length on protein retention on a strong cation exchange adsorbent10.1021/ac049462b ↗
42. Type IV collagenases (MMP-2 and MMP-9) and their substrates–intracellular proteins, hormones, cytokines, chemokines and their receptors
43. Matrix metalloproteinases (MMPs), the main extracellular matrix (ECM) enzymes in collagen degradation, as a target for anticancer drugs10.3109/14756366.2016.1161620 ↗
44. An improved hydrophilicity via electrospinning for enhanced cell attachment and proliferation10.1002/jbm.b.30484 ↗
45. Engineering the cell–material interface for controlling stem cell adhesion, migration, and differentiation10.1016/j.biomaterials.2011.02.004 ↗
46. Modulation of cell adhesion, proliferation and differentiation on materials designed for body implants10.1016/j.biotechadv.2011.06.004 ↗
47. Surface engineering of cardiovascular stent with endothelial cell selectivity for in vivo re-endothelialisation10.1016/j.biomaterials.2012.12.036 ↗
48. Two-tier hydrogel degradation to boost endothelial cell morphogenesis10.1016/j.biomaterials.2011.08.078 ↗
49. Design of polymeric gene carriers for effective intracellular delivery10.1016/j.tibtech.2018.02.006 ↗
50. Multi-targeting peptides for gene carriers with high transfection efficiency10.1039/C7TB02012K ↗
51. Bioreducible Zinc(II)-coordinative polyethylenimine with low molecular weight for robust gene delivery of primary and stem cells10.1021/jacs.6b13337 ↗
52. Highly branched poly(β-amino ester) delivery of minicircle DNA for transfection of neurodegenerative disease related cells10.1038/s41467-019-11190-0 ↗
53. Biodegradable highly branched poly(β-amino ester)s for targeted cancer cell gene transfection10.1021/acsbiomaterials.6b00503 ↗
54. Reduction-responsive nucleic acid delivery systems to prevent in-stent restenosis in rabbits10.1021/acsami.9b08544 ↗
55. Preparation of an Arg-Glu-Asp-Val peptide density gradient on hyaluronic acid-coated poly(ε-caprolactone) film and its influence on the selective adhesion and directional migration of endothelial cells10.1021/acsami.6b09375 ↗
56. Protective role of endothelial nitric oxide synthase10.1002/path.1250 ↗
57. Dysfunction of endothelial nitric oxide synthase and atherosclerosis10.1161/01.ATV.0000125114.88079.96 ↗
58. Direct evidence for the importance of endothelium-derived nitric oxide in vascular remodeling10.1172/JCI1699 ↗
59. Nitric oxide-generating vasodilators and 8-bromo-cyclic guanosine monophosphate inhibit mitogenesis and proliferation of cultured rat vascular smooth muscle cells10.1172/JCI114081 ↗
60. An injectable conductive hydrogel encapsulating plasmid DNA-eNOs and ADSCs for treating myocardial infarction10.1016/j.biomaterials.2018.01.021 ↗
61. Platelet membrane biomimetic magnetic nanocarriers for targeted delivery and in situ generation of nitric oxide in early ischemic stroke10.1021/acsnano.9b08587 ↗
62. Cascaded bio-responsive delivery of eNOS gene and ZNF580 gene to collaboratively treat hindlimb ischemia via pro-angiogenesis and anti-inflammation10.1039/D0BM01573C ↗
63. Nitric oxide activates PI3-K and MAPK signalling pathways in human and rat vascular smooth muscle cells: influence of insulin resistance and oxidative stress10.1016/j.atherosclerosis.2011.01.019 ↗
64. Shear stress induces synthetic-to-contractile phenotypic modulation in smooth muscle cells via peroxisome proliferator-activated receptor alpha/delta activations by prostacyclin released by sheared endothelial cells10.1161/CIRCRESAHA.109.193656 ↗
65. Regulation of vascular smooth muscle cell phenotype in three-dimensional coculture system by Jagged1-selective Notch3 signaling10.1089/ten.tea.2013.0268 ↗
66. Mussel-inspired dopamine-CuII coatings for sustained in situ generation of nitric oxide for prevention of stent thrombosis and restenosis10.1016/j.biomaterials.2018.12.020 ↗
67. The effect of hypoxia-mimicking responses on improving the regeneration of artificial vascular grafts10.1016/j.biomaterials.2021.120746 ↗
68. Electrospun vascular scaffold for cellularized small diameter blood vessels: a preclinical large animal study10.1016/j.actbio.2017.06.027 ↗
69. Local delivery of dual microRNAs in trilayered electrospun grafts for vascular regeneration10.1021/acsami.9b19452 ↗
70. Development and evaluation of in vivo tissue engineered blood vessels in a porcine model10.1016/j.biomaterials.2015.10.023 ↗