Convenient in situ synthesis of injectable lysine-contained peptide functionalized hydrogels for spinal cord regeneration
Source abstract
Abstract
Injectable hydrogels provide a promising strategy to fill the irregular cystic cavity in spinal cord injury. However, a simple and convenient functionalization approach of injectable hydrogels is still a huge challenge. Herein, we report a facile in situ synthetic strategy of injectable lysine-contained peptide functionalized hydrogels for the treatment of spinal cord injury. The ibuprofen-KYIGSRK conjugated hydrogels are in situ synthesized via in situ Michael addition without catalysts and additional reaction-steps. Ibuprofen-KYIGSRK conjugated soft hydrogels promote the adhesion and neurite extension of DRG neurons by upregulating of Sall3, Pou3f3, Lhx5, Shank1 and Sall1. The long-term implanted ibuprofen-KYIGSRK hydrogel is injected into the spinal cord injured site for promoting motor functional recovery. Increased neurites and decreased glial scars are detected with effective functional improvements in footprint. This injectable hydrogel can not only fill the irregular cavities for inhibiting the glial scar formation, but also significantly suppress inflammatory responses and promote nerve regeneration. This study supplies a convenient in situ synthetic route of injectable peptide functionalized hydrogels for the treatment of spinal cord injury or other biomedical applications.
Source: expertdata · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Nerve injury and regeneration · Spinal Cord Injury Research · Nerve Injury and Rehabilitation
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (48)
Show source-provided references
1. Biomimetic 3D-printed scaffolds for spinal cord injury repair10.1038/s41591-018-0296-z ↗
2. Recent trends in the development of peptide and protein-based hydrogel therapeutics for the healing of CNS injury10.1039/D0SM00885K ↗
3. Courtine · Spinal cord repair: advances in biology and technology, Nat. Med · 2019 · 25 · 898
4. Traumatic and nontraumatic spinal cord injury: pathological insights from neuroimaging10.1038/s41582-019-0270-5 ↗
5. The leading edge: Emerging neuroprotective and neuroregenerative cell-based therapies for spinal cord injury10.1002/sctm.19-0135 ↗
6. Huntemer-Silveira · Strategies for Oligodendrocyte and Myelin Repair in Traumatic CNS Injury, Front. Cell. Neurosci. · 2021 · 14
7. Glucocorticoids promote apoptosis of proinflammatory monocytes by inhibiting ERK activity10.1038/s41419-018-0332-4 ↗
8. Bioactive Elastic Scaffolds Loaded with Neural Stem Cells Promote Rapid Spinal Cord Regeneration10.1021/acsbiomaterials.0c01057 ↗
9. An elastic gel consisting of natural polyphenol and pluronic for simultaneous dura sealing and treatment of spinal cord injury10.1016/j.jconrel.2020.04.042 ↗
10. Pluripotent Stem Cell-Based Cell Therapy- Promise and Challenges10.1016/j.stem.2020.09.014 ↗
11. Injectable biomaterials for translational medicine10.1016/j.mattod.2019.04.020 ↗
12. Advanced biomaterials for repairing the nervous system: what can hydrogels do for the brain?10.1016/j.mattod.2014.05.011 ↗
13. The effect of a nanofiber-hydrogel composite on neural tissue repair and regeneration in the contused spinal cord10.1016/j.biomaterials.2020.119978 ↗
14. Small molecules combined with collagen hydrogel direct neurogenesis and migration of neural stem cells after spinal cord injury10.1016/j.biomaterials.2020.120479 ↗
15. Vascularization of self-assembled peptide scaffolds for spinal cord injury repair10.1016/j.actbio.2019.12.033 ↗
16. Moving beyond the glial scar for spinal cord repair10.1038/s41467-019-11707-7 ↗
17. Two-Dimensional-Germanium Phosphide-Reinforced Conductive and Biodegradable Hydrogel Scaffolds Enhance Spinal Cord Injury Repair10.1002/adfm.202104440 ↗
18. Highly Permeable DNA Supramolecular Hydrogel Promotes Neurogenesis and Functional Recovery after Completely Transected Spinal Cord Injury10.1002/adma.202102428 ↗
19. Tunable Growth Factor Delivery from Injectable Hydrogels for Tissue Engineering10.1021/ja210638x ↗
20. Reactive oxygen species (ROS): utilizing injectable antioxidative hydrogels and ROS-producing therapies to manage the double-edged sword10.1039/D1TB00728A ↗
21. Injectable Hypoxia-Induced Conductive Hydrogel to Promote Diabetic Wound Healing10.1021/acsami.0c13197 ↗
22. Inhaled Nanoformulated mRNA Polyplexes for Protein Production in Lung Epithelium
23. Brain-Targeted Dual Site-Selective Functionalized Poly(beta-Amino Esters) Delivery Platform for Nerve Regeneration10.1021/acs.nanolett.1c00175 ↗
24. Synthesis and Evaluation of Cytocompatible Alkyne-Containing Poly(beta-amino ester)-Based Hydrogels Functionalized via Click Reaction10.1021/acsmacrolett.0c00545 ↗
25. Hydrogel vectors based on peptide and peptide-like substances: For treating bacterial infections and promoting wound healing
26. A 3D-printable, glucose -sensitive and thermoresponsive hydrogel as sacrificial materials for constructs with vascular-like channels
27. Trigger-Responsive Poly(beta-amino ester) Hydrogels10.1021/mz500277j ↗
28. One-Step Injectable and Bioreducible Poly(beta-Amino Ester) Hydrogels as Controlled Drug Delivery Platforms10.1021/acsapm.9b00287 ↗
29. Injectable hyperbranched poly(beta-amino ester) hydrogels with on-demand degradation profiles to match wound healing processes10.1039/C7SC03913A ↗
30. Effect of Dehydrothermal Treatment on the Mechanical Properties and Biocompatibility of Plaster of Paris-CaCO3 Hydrogel Loaded With Cinnamaldehyde for Biomedical Purposes
31. Biocompatible smart cellulose nanofibres for sustained drug release via pH and temperature dual-responsive mechanism10.1016/j.carbpol.2020.116876 ↗
32. Assessing the combination of magnetic field stimulation, iron oxide nanoparticles, and aligned electrospun fibers for promoting neurite outgrowth from dorsal root ganglia in vitro10.1016/j.actbio.2021.06.049 ↗
33. Loss of the Sall3 gene leads to palate deficiency, abnormalities in cranial nerves, and perinatal lethality10.1128/MCB.24.16.7102-7112.2004 ↗
34. Sall1 regulates mitral cell development and olfactory nerve extension in the developing olfactory bulb10.1093/cercor/bhm191 ↗
35. De Novo Variants Disturbing the Transactivation Capacity of POU3F3 Cause a Characteristic Neurodevelopmental Disorder10.1016/j.ajhg.2019.06.007 ↗
36. Control of hippocampal morphogenesis and neuronal differentiation by the LIM homeobox gene Lhx510.1126/science.284.5417.1155 ↗
37. SHANK1 Deletions in Males with Autism Spectrum Disorder10.1016/j.ajhg.2012.03.017 ↗
38. Counteracting roles of MHCI and CD8(+) T cells in the peripheral and central nervous system of ALS SOD1(G93A) mice10.1186/s13024-018-0271-7 ↗
39. 3D Printed Neural Regeneration Devices10.1002/adfm.201906237 ↗
40. A multi-modal delivery strategy for spinal cord regeneration using a composite hydrogel presenting biophysical and biochemical cues synergistically10.1016/j.biomaterials.2021.120971 ↗
41. Cytokine mechanisms of central sensitization: Distinct and overlapping role of interleukin-1 beta, interleukin-6, and tumor necrosis factor-beta in regulating synaptic and neuronal activity in the superficial spinal cord10.1523/JNEUROSCI.3338-07.2008 ↗
42. Construction of injectable silk fibroin/polydopamine hydrogel for treatment of spinal cord injury10.1016/j.cej.2020.125795 ↗
43. Neutrophil Decoys with Anti-Inflammatory and Anti-Oxidative Properties Reduce Secondary Spinal Cord Injury and Improve Neurological Functional Recovery10.1002/adfm.202102912 ↗
44. Selective Modulation of A1 Astrocytes by Drug-Loaded Nano-Structured Gel in Spinal Cord Injury10.1021/acsnano.9b05579 ↗
45. Supramolecular Self-Assembly-Facilitated Aggregation of Tumor-Specific Transmembrane Receptors for Signaling Activation and Converting Immunologically Cold to Hot Tumors
46. Conotoxin loaded dextran microgel particles alleviate effects of spinal cord injury by inhibiting neuronal excitotoxicity
47. Cell-Free Extracts from Human Fat Tissue with a Hyaluronan-Based Hydrogel Attenuate Inflammation in a Spinal Cord Injury Model through M2 Microglia/Microphage Polarization
48. Diacerein Loaded Poly (Styrene Sulfonate) and Carbon Nanotubes Injectable Hydrogel: An Effective Therapy for Spinal Cord Injury Regeneration