Effects of palygorskite on steroid-transformation in Mycobacterium neoaurum
Source abstract
Abstract
The low availability of hydrophobic substrates limited the bioproduction of steroid medications. As palygorskite (Pal) has excellent adsorption and dispersion capacity, Pal and modified Pal were incubated with Mycobacterium neoaurum TCCC 11979 ( MNR ) to investigate their effects on phytosterols (PS) biotransformation. The production of the steroid medicine intermediate androst-4-ene-3, 17-dione (AD) was remarkably improved with Pal or modified Pal as a carrier in PS biotransformation. Entropy and gas chromatography analyses confirmed that Pal samples increased the dispersion and adsorption of PS. Meanwhile, the cell permeability of MNR by nucleic acid improved. Among Pal samples, KH550 modified Pal showed superior properties, which results in the good dispersion of substrate and effectively improves the cell permeability without affecting cell growth (confirmed by TEM). Therefore, Pal, particularly KH550 modified Pal, can be used as a carrier in AD production by MNR. The data obtained will expand the application of Pal in the pharmaceutical industry and improve our understanding of the mechanism underlying the effect of Pal on the bioconversion of hydrophobic compounds.
Source: expertdata · Quality: candidate unverified · Source record ↗
Research context
OpenAlex topics: Steroid Chemistry and Biochemistry · Pharmacogenetics and Drug Metabolism
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (42)
Show source-provided references
1. Effect of Tween 80 on 9α-steroid hydroxylating activity and ultrastructural characteristics of Rhodococcus sp. cells10.1007/s11274-009-0263-4 ↗
2. Sorption of p-nitrophenol by anion-cation modified palygorskite10.1016/j.jhazmat.2009.02.105 ↗
3. Powder-diffraction patterns and structural models for palygorskite
4. Improvement of Escherichia coli growth by kaolinite10.1016/j.clay.2009.01.010 ↗
5. A comparative study about superamphiphobicity and stability of superamphiphobic coatings based on palygorskite10.1016/j.clay.2018.07.034 ↗
6. Adsorption of Pb (II) on palygorskite from aqueous solution: effects of pH, ionic strength and temperature10.1016/j.clay.2009.04.009 ↗
7. Acid treatment of south Tunisian palygorskite: removal of Cd (II) from aqueous and phosphoric acid solutions10.1016/j.desal.2009.01.043 ↗
8. Properties and applications of palygorskite-sepiolite clays10.1180/claymin.1996.031.4.01 ↗
9. Sorption of red dyes on palygorskite: Synthesis and stability of red/purple Mayan nanocomposites10.1016/j.micromeso.2010.12.004 ↗
10. The Entropy of an image
11. Solvent-tolerant bacteria for biotransformations in two-phase fermentation systems10.1007/s00253-006-0833-4 ↗
12. Influence of differently modified palygorskites in the immobilization of a lipase10.1016/j.molcatb.2007.12.025 ↗
13. The relationship between membrane damage, release of protein and loss of viability in Escherichia coli exposed to high hydrostatic pressure10.1016/j.ijfoodmicro.2009.11.020 ↗
14. Acid activated clays: materials in continuous demand10.1016/j.clay.2016.05.001 ↗
15. Pd modified kaolinite nanocomposite as a hydrogenation catalyst10.1039/C5RA25387J ↗
16. Assembling strategy to synthesize palladium modified kaolin nanocomposites with different morphologies10.1038/srep13763 ↗
17. Effect of dry grinding on the microstructure of palygorskite and adsorption efficiency for methylene blue10.1016/j.powtec.2012.03.049 ↗
18. Organo-functionalization: an effective method in enhancing the separation and antifouling performance of thin-film nanocomposite membranes by improving the uniform dispersion of palygorskite nanoparticles10.3390/membranes11110889 ↗
19. Green synthesis of DyBa2Fe3O7.988/DyFeO3 nanocomposites using almond extract with dual eco-friendly applications: Photocatalytic and antibacterial activities10.1016/j.ijhydene.2022.02.175 ↗
20. Enhanced biotransformation of sitosterol to androstenedione by Mycobacterium sp. using cell wall permeabilizing antibiotics10.1007/s10295-008-0419-5 ↗
21. Evaluation of deep eutectic solvents as co-solvent for steroids 1-en-dehydrogenation biotransformation by Arthrobacter simplex10.1002/jctb.4691 ↗
22. Effect of montmorillonite and humate on growth and metabolic activity of some actinomycetes10.1016/0038-0717(76)90042-0 ↗
23. Molecular cloning, expression in Streptomyces lividans, and analysis of a gene cluster from Arthrobacter simplex encoding 3-ketosteroid-Δ1-dehydrogenase, 3-ketosteroid-Δ5-isomerase and a hypothetical regulatory protein10.1111/j.1365-2958.1995.tb02359.x ↗
24. Modification of the textural properties of palygorskite through microwave assisted acid treatment. Influence of the octahedral sheet composition
25. Microcalorimetric investigation on the metabolic activity of bacillus thuringiensis as influenced by kaolinite, montmorillonite and goethite10.1016/j.clay.2007.01.015 ↗
26. Structural, electrokinetic and surface properties of activated palygorskite for environmental application10.1016/j.clay.2016.07.012 ↗
27. Effects of hydroxypropyl-β-cyclodextrin on cell growth, activity, and integrity of steroid-transforming Arthrobacter simplex and Mycobacterium sp10.1007/s00253-011-3214-6 ↗
28. Hydroxypropyl-β-cyclodextrin-mediated alterations in cell permeability, lipid and protein profiles of steroid-transforming Arthrobacter simplex10.1007/s00253-014-6089-5 ↗
29. Effect of attapulgite on cell activity of steroid-transforming Arthrobacter simplex
30. Effects of two kinds of imidazolium-based ionic liquids on the characteristics of steroid-transformation Arthrobacter simplex10.1186/s12934-016-0518-3 ↗
31. A new technique for promoting cyclic utilization of cyclodextrins in biotransformation10.1007/s10295-016-1856-1 ↗
32. Influence of clay minerals on microorganisms. I. Montmorillonite and kaolinite on bacteria10.1139/m66-078 ↗
33. Cofactor engineering to regulate NAD+/NADH ratio with its application to phytosterols biotransformation10.1186/s12934-017-0796-4 ↗
34. Microbial physiology of side-chain degradation of sterols
35. Investigation on the effect of poly (butylmethacrylate)/ attapulgite nanocomposites for oil absorption10.2175/106143016X14609975747928 ↗
36. Freeze-drying: a versatile method to overcome re-aggregation and improve dispersion stability of palygorskite for sustained release of ofloxacin10.1016/j.clay.2013.11.017 ↗
37. Construction of automated high-throughput screening method for finding efficient 3-ketosteroid 1, 2-dehydrogenating strains10.1007/s00253-022-12190-9 ↗
38. Genetic differences in ksdD influence on the ADD/AD ratio of Mycobacterium neoaurum10.1007/s10295-014-1577-2 ↗
39. Adsorption of 17 beta-estradiol by a novel attapulgite/biochar nanocomposite: characteristics and influencing factors10.1016/j.psep.2018.10.022 ↗
40. Control sonochemical parameter to prepare pure Zn0.35Fe2.65O4 nanostructures and study their photocatalytic activity10.1016/j.ultsonch.2019.104619 ↗
41. Highly effective removal of Methylene Blue using functionalized attapulgite via hydrothermal process10.1016/j.jes.2014.12.014 ↗
42. Removal of gaseous carbon bisulfide using dielectric barrier discharge plasmas combined with TiO2 coated attapulgite catalyst10.1016/j.cej.2013.03.107 ↗