Immobilization of Rhus vernicifera laccase on sepiolite; effect of chitosan and copper modification on laccase adsorption and activity
Source abstract
Abstract
Abstract The enzyme laccase, a multi-copper oxidase found in many plants and fungi, can be used in water treatment processes for the removal of pollutants. Commercially available laccase, derived from Rhus vernicifera, was adsorbed on sepiolite, sepiolite modified with chitosan, sepiolite plus Cu(II), and sepiolite modified with both chitosan and Cu(II) to investigate enzymatic activity. Adsorption of laccase on unmodified sepiolite increased its activity by 250 ± 40% compared to the non-adsorbed enzyme, whereas for sepiolite-Cu–chitosan and sepiolite–chitosan the activity was enhanced by up to 700% and 500%, respectively. The stronger enhancement for the Cu-containing adsorbent suggests that exchangeable Cu has an effect on the adsorbed laccase. Desorption of the adsorbed laccase was
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Research context
OpenAlex topics: Enzyme-mediated dye degradation · Nanoparticles: synthesis and applications · Carbon and Quantum Dots Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (27)
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1. Fungal laccases – occurrence and properties10.1111/j.1574-4976.2005.00010.x ↗
2. Conjugation of laccase from the white rot fungus Trametes versicolor to chitosan and its utilization for the elimination of triclosan10.1016/j.biortech.2010.09.080 ↗
3. Immobilization of fungal laccase onto a nonionic surfactant-modified clay material: application to PAH degradation
4. Microfibrous chitosan - sepiolite nanocomposites10.1021/cm0523642 ↗
5. Isothermal and non-isothermal bioreactors in the detoxification of waste waters polluted by aromatic compounds by means of immobilised laccase from Rhus vernicifera10.1016/j.molcatb.2003.11.008 ↗
6. Recent developments and applications of immobilized laccase10.1016/j.biotechadv.2012.02.013 ↗
7. Laccases: a never-ending story10.1007/s00018-009-0169-1 ↗
8. Silica-sepiolite nanoarchitectures10.1166/jnn.2013.7429 ↗
9. Laccase activity and laccase inhibitors Laccase activity tests and laccase inhibitors10.1016/S0168-1656(00)00208-X ↗
10. Immobilization of laccase on a novel ZnO/SiO 2 nano-composited support for dye decolorization10.1088/1757-899X/87/1/012033 ↗
11. Studies on acetone powder and purified Rhus Laccase immobilized on zirconium chloride for oxidation of phenols10.1155/2012/375309 ↗
12. Immobilization of laccase by alginate-chitosan microcapsules and its use in dye decolorization10.1007/s11274-006-9205-6 ↗
13. Mangiafico · 2016
14. Gene structure and molecular analysis of the laccase-like multicopper oxidase (LMCO) gene family in Arabidopsis thaliana10.1007/s00425-004-1472-6 ↗
15. Fungal laccase in the textile industry
16. The use of clay-polymer nanocomposites in wastewater pretreatment10.1100/2012/498503 ↗
17. Use of CV- and TPP-montmorillonite for the removal of priority pollutants from water10.1016/j.clay.2006.04.016 ↗
18. Clarification of olive mill and winery wastewater by means of clay-polymer nanocomposites10.1016/j.scitotenv.2012.10.031 ↗
19. A continuous-flow device for photocatalytic degradation and full mineralization of priority pollutants in water10.1080/19443994.2015.1077749 ↗
20. Adsorption of monovalent organic cations on sepiolite; experimental results and model calculations10.1346/CCMN.1998.0460313 ↗
21. Structural fluorine in sepiolite10.1346/CCMN.1990.0380109 ↗
22. An electrochemical-spectrophotometrical study of the oxidized forms of the mediator 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) produced by immobilized laccase10.1016/j.jelechem.2005.01.025 ↗
23. Fungal laccases and their applications in bioremediation10.1155/2014/163242 ↗
24. Activity of laccase immobilized on TiO2-Montmorillonite complexes10.3390/ijms140612520 ↗
25. Hierarchically structured bioactive foams based on polyvinyl alcohol–sepiolite nanocomposites10.1039/c3tb00580a ↗
26. A study of a series of recombinant fungal laccases and bilirubin oxidase that exhibit significant differences in redox potential, substrate specificity, and stability10.1016/0167-4838(95)00210-3 ↗
27. Immobilization and characterization of laccase from Chinese Rhus vernicifera on modified chitosan10.1016/j.procbio.2006.01.018 ↗