Enzyme immobilization. Part 3
Source abstract
Abstract
Immobilization of α-amylase on bentonite modified with cetyl trimethylammonium bromide (CTMAB) below and above the critical micelle concentration (CMC) was studied and compared to α-amylase adsorbed on Na-bentonite. The highest enzyme activity for free and immobilized α-amylase was obtained in phosphate buffer 0.1 M at pH 6.2. The kinetic study of starch hydrolysis by the immobilized α-amylase was investigated and compared with the free α-amylase. A 6.2-fold decrease in V max and 4-fold increase in K m were observed for the immobilized α-amylase on Na-bentonite. A 7.1-fold decrease in V max and 4.2-fold increase in K m were observed for the α-amylase immobilized on modified bentonite with monolayer surfactant (BMS). Approximately no change in V max and K m was observed for the enzyme immobilized on bentonite with bilayer surfactant coverage (BBS) in comparison with the free α-amylase.
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Research context
OpenAlex topics: Enzyme Production and Characterization · Minerals Flotation and Separation Techniques · Enzyme Catalysis and Immobilization
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
References (33)
Show source-provided references
1. Stability of α-amylase immobilized on poly(methyl methacrylate-acrylic acid) microspheres10.1016/S0168-1656(97)00179-X ↗
2. α-Amylase inactivation during rice starch hydrolysis10.1016/j.procbio.2004.06.006 ↗
3. Improved stability of lignin proxidase by immobilization
4. Immobilization of a thermostable α-amylase onto reactive membranes: kinetics characterization and application to continuous starch hydrolysis10.1016/S0308-8146(03)00283-8 ↗
5. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding10.1016/0003-2697(76)90527-3 ↗
6. Activities, stabilities, and reaction kinetics of three free and chitosan–clay composite immobilized enzymes10.1016/j.enzmictec.2004.06.013 ↗
7. Preparation and characterization of alkaline protease immobilized on vermiculite10.1016/S0032-9592(97)00043-5 ↗
8. Immobilization of α-amylase to temperature-responsive polymers by single and multiple point attachments10.1002/(SICI)1097-4660(199708)69:4<421::AID-JCTB730>3.0.CO;2-3 ↗
9. Urea biosensors based on immobilization of urease into two oppositely charged clays (laponite and Zn–Al layered double hydroxides)10.1021/ac025627+ ↗
10. Immobilization of urease and estimation of effective diffusion coefficients of urea in HEMA and VP copolymer matrices10.1002/pi.1994.210350404 ↗
11. Stabilization of α-amylase by using anionic surfactant during the immobilization process10.1016/j.radphyschem.2004.12.006 ↗
12. Adsorption and binding of protein on “clean” (homoionic) and “dirty” (coated with Fe oxyhydroxides) montmorillonite, illite and kaolinite10.1016/0038-0717(89)90080-1 ↗
13. Enzyme immobilization Part 1. Modified bentonite as a new and efficient support for immobilization of Candida rugosa lipase10.1016/j.clay.2008.09.008 ↗
14. Enzyme immobilization: part 2 immobilization of alkaline phosphatase on Na-bentonite and modified bentonite10.1016/j.clay.2008.09.011 ↗
15. Effect of soils on the behavior of immobilized enzymes10.2136/sssaj1994.03615995005800060014x ↗
16. Water-insoluble derivatives of naringinase10.1016/0020-711X(71)90082-6 ↗
17. Enzymes immobilized on montmorillonite K 10: effect of adsorption and grafting on the surface properties and the enzyme activity10.1016/j.clay.2006.04.007 ↗
18. Microbial α-amylases: a biotechnological perspective10.1016/S0032-9592(03)00053-0 ↗
19. α-Amylase immobilized on bulk acoustic-wave sensor by UV-curing coating10.1016/S1369-703X(00)00068-1 ↗
20. Immobilization of α-amylase on Cu2+ chelated poly(ethylene glycol dimethacrylate-n-vinyl imidazole) matrix via adsorption10.1016/j.reactfunctpolym.2004.08.008 ↗
21. Hydrolysis of starches by the action of an α-amylase from Bacillus subtilis10.1016/j.procbio.2003.07.003 ↗
22. Ladd, J.N., 1985. “Soil enzymes”, in: D. Vaughan, and R.E. Malcom (Eds), Soil Organic Matter and Biological Activity: Development in Plant and Soil Science; Vol 16, Kluwer academic publishers group, martinus nijhoff Dr W. Junk publishers, Dordrecht, The Netherlands, PP. 175–221.10.1007/978-94-009-5105-1_6 ↗
23. Covalent immobilization of aminoacrylase to alginate for 2-phenylalanine production10.1002/jctb.280580109 ↗
24. Enzymes covalently bound to various polymers and the effects on the properties of these enzymes10.1002/polc.5070300163 ↗
25. Use of dinitrisalisylic acid reagent for determination of reducing sugar10.1021/ac60147a030 ↗
26. Stability of industrial enzymes
27. High-throughput hydrolysis of starch during permeation across α-amylase-immobilized porous hollow-fiber membranes10.1016/S0969-806X(01)00222-5 ↗
28. Deamination of aspartic acid by aspartase–Ca–montmorillonite complex10.1016/1381-1169(95)00262-6 ↗
29. A comparative study of immobilized amyloglucosidase in a packed bed reactor and a continuous feed stirred tank reactor10.1002/bit.260130302 ↗
30. An overview of the microbial α-amylase family10.5897/AJB2003.000-1119 ↗
31. Immobilization of α-amylase on zirconia: a heterogeneous biocatalyst for starch hydrolysis10.1016/j.catcom.2006.07.009 ↗
32. Purification and properties of an extra cellular α-amylase from Thermus sp
33. Covalent immobilization of α-amylase onto poly(2-hydroxyethyl methacrylate) and poly(styrene-2-hydroxyethyl methacrylate) microspheres and the effect of Ca2+ ions on the enzyme activity10.1016/S0308-8146(99)00184-3 ↗