The process of EDC-NHS cross-linking of reconstituted collagen fibres increases collagen fibrillar order and alignment
Source abstract
Abstract
We describe the production of collagen fibre bundles through a multi-strand, semi-continuous extrusion process. Cross-linking using an EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide), NHS (N-hydroxysuccinimide) combination was considered. Atomic Force Microscopy and Raman spectroscopy focused on how cross-linking affected the collagen fibrillar structure. In the cross-linked fibres, a clear fibrillar structure comparable to native collagen was observed which was not observed in the non-cross-linked fibre. The amide III doublet in the Raman spectra provided additional evidence of alignment in the cross-linked fibres. Raman spectroscopy also indicated no residual polyethylene glycol (from the fibre forming buffer) or water in any of the fibres.
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Research context
OpenAlex topics: Collagen: Extraction and Characterization · Periodontal Regeneration and Treatments · Bone Tissue Engineering Materials
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Materials Science and Metallurgy, University of Cambridge 1 , Cambridge, United Kingdom
Tigenix Ltd. 2 , Byron House, Cambridge Business Park, Cambridge, United Kingdom
Funding
Engineering and Physical Sciences Research Council (EPSRC)
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