Source abstract
Abstract
Abstract Conjugated polymer particles are brightly fluorescing and stable materials for live cell imaging. Combination of conjugated polymers with a whispering gallery mode (WGM) resonator allows laser emission from microscale particles. Once internalized by cells, the mode pattern of the laser emission can be used for tagging and tracking, as each laser spectrum represents a bar code to identify individual cells. However, currently these particle systems are limited by their large size, which might interfere with cellular functions. Here, stimulated resonance Raman scattering (SRRS) in small conjugated polymer microparticles is presented as a new method for generating narrow emission as an alternative to WGM‐based laser emission. This opens up spectral range for multiplexing optical readout and multicolor imaging of live cells. The synthesis of monodisperse micrometer‐sized poly(fluorene‐co‐divinylbenzene) particles is discussed and their WGM and SRRS emission are characterized. Finally, how these particles and their emission can be employed in live cell imaging and tagging is showcased.
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Research context
OpenAlex topics: Spectroscopy Techniques in Biomedical and Chemical Research · Advanced Fluorescence Microscopy Techniques · Gold and Silver Nanoparticles Synthesis and Applications
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Affiliations
Institute of Organic and Macromolecular Chemistry Ulm University Albert‐Einstein‐Allee 11 89081 Ulm Germany
Institute of Molecular Virology Ulm University Medical Center 89081 Ulm Germany
SUPA School of Physics and Astronomy University of St Andrews North Haugh, St Andrews Fife KY16 9SS UK
Centre for Nanobiophotonics Department of Chemistry University of Cologne Greinstr. 4‐6 50939 Köln Germany
DWI Leibniz Institute for Interactive Materials Forckenbeckstraße 50 52074 Aachen Germany
References (30)
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