Trends in Nanophotonics‐Enabled Optofluidic Biosensors

Abstract Optofluidic sensors integrate photonics with micro/nanofluidics to realize compact devices for the label‐free detection of molecules and the real‐time monitoring of dynamic surface binding events with high specificity, ultrahigh sensitivity, low detection limit, and multiplexing capability. Nanophotonic structures composed of metallic and/or dielectric building blocks excel at focusing light into ultrasmall volumes, creating enhanced electromagnetic near‐fields ideal for amplifying the molecular signal readout. Furthermore, fluidic control on small length scales enables precise tailoring of the spatial overlap between the electromagnetic hotspots and the analytes, boosting light‐matter interaction, and can be utilized to integrate advanced functionalities for the pre‐treatment of samples in real‐world‐use cases, such as purification, separation, or dilution. In this review, the authors highlight current trends in nanophotonics‐enabled optofluidic biosensors for applications in the life sciences while providing a detailed perspective on how these approaches can synergistically amplify the optical signal readout and achieve real‐time dynamic monitoring, which is crucial in biomedical assays and clinical diagnostics.