Tunable Dynamic Speckle Generation for Random Illumination Microscopy

ABSTRACT Speckled illumination enhances widefield fluorescence microscopy by enabling optical sectioning and super resolution. In random illumination microscopy, sequences of speckled illumination patterns are used to excite fluorescent samples and images are reconstructed based on a statistical analysis of the intensity fluctuations. Although random illumination microscopy has been shown to give excellent performance, its widespread implementation is hindered by the high cost and complexity of the generation of suitable speckled illumination patterns, which is typically achieved using digital micro‐mirror devices or spatial light modulators. Here, we present a zwitterion‐doped liquid crystal device capable of generating independent, high‐contrast speckle patterns with a tunable decorrelation time in the 0.1 s – 0.1 ms range under visible laser illumination. This liquid crystal based dynamic speckle generator is applied to widefield random illumination fluorescence microscopy of tissue and cell samples, where it enables optical sectioning with a 2 axial resolution, and a 1.5 ‐ fold improvement in lateral spatial resolution. Owing to its low cost and simplicity, this liquid crystal speckle generator offers an attractive alternative to digital micro‐mirror and spatial light modulator devices for implementing widefield random illumination microscopy.