Engineered Microfluidic Organoid Systems: New Paradigms for Menopause Mechanism Research and Personalized Medicine

ABSTRACT Menopausal syndrome is a group of multi‐organ and multi‐system diseases that occur in women before and after menopause. Although the emergence of organoid technology has better solved the problem of expressing cell–cell interactions in vitro, for the study of multi‐organ diseases, organoid technology may not be able to simulate the interactions between organs. Thus, by combining microfluidics with organoids, precise control of the liquid medium in the system can be achieved, allowing for real‐time quantitative simulation of material exchange and physiological mechanisms within the human body's internal environment. With the continuous advancement of the above‐mentioned technologies, it is possible to further understand the epigenetic changes of multiple organs before and after menopause in vitro, and provide new ideas for the precise implementation of hormone replacement therapy for menopausal syndrome.