Localization of Multiple Hydrogels with Multicube Platform Spatially Guides 3d Tissue Morphogenesis In Vitro

In the original version of this article, there were errors in the concentration units within the article and errors in the figure captions.These errors were made by the editorial team during the production process.In sub-section 2.4 "Localization of Growth Factor in Hydrogel Composition Guided the Location-Specific Branching Morphogenesis of a Single Tissue", the correct unit in the following sentence is as follows:On the other hand, supplementing Matrigel with 2.5 10 -9 M heparin-binding EGF-like growth factor (HBEGF), an EGFR-binding ligand with a higher affinity to Matrigel, [35,36] successfully generated NHBE branching under an EGF(-) medium over 3 days (Figure 4A, 4th column) In section 5, sub-section "3D Spheroidal Culture in Uniform Hydrogel", the correct unit in the following sentence is as follows:To compare the retention of growth factors in Matrigel, NHBE clusters were also cultured in (i) a normal GFR-Matrigel, i.e., EGF(-) Matrigel or control Matrigel; (ii) a GFR-Matrigel premixed with EGF at 2.5 10 -9 M final concentration, i.e., EGF(+) Matrigel; and (iii) a GFR-Matrigel premixed with human recombinant heparin-binding EGF-like growth factor (HBEGF; E4643, Sigma) at 2.5 10 -9 M final concentration, i.e., HBEGF(+) Matrigel.These three Matrigel conditions were cultured under EGF(-) medium submersion.In section 5, sub-section "3D Localization Experiment: Tissue Branching-Pattern Model", the correct unit in the following sentence is as follows:For HBEGF localization, HBEGF was premixed to Matrigel at the final concentration of 2.5 10 -9 M under an ice bath.In section 5, sub-section "Immunofluorescence Staining", the correct unit in the following sentence is as follows:Then, it was washed once with IF solution and stained with 600 10 -9 M DAPI in DPBS for 1 h.In section 5, sub-section "Immunofluorescence Staining", the correct unit in the following sentence is as follows:After blocking, the samples from the tissue branching-pattern model were stained with 600 10 -9 M DAPI in DPBS and 50 10 -6 M phalloidin (PhenoVue Fluor 488; CP24881, PerkinElmer) together for 1 h.