DataVXAdvanced Materials TechnologiesPaper
journal article·2025-05Open access

A Protocol Using Compact 3D Printed Micro‐Optical Elements for Protein Identification from Low‐Intensity Amino‐Acid Raman Signals

Jannis Weinacker, Bikash Kumar Bhandari, Alba Viejo Rodriguez, Charlotte West, Francesco De Angelis, Francesco Tantussi, Nicolò Maccaferri, Nick Goldman, Martin Wegener

Advanced Materials Technologies· Volume 10· Issue 9
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Source abstract

Abstract

Abstract When performing optical high‐speed single‐molecule spectroscopy and identification, low signal intensities pose a challenge. Fortunately, for many applications, the number of possible molecules in the specimen is small or limited. For such cases, a protocol is presented that uses only a small number of very sensitive hence expensive detectors. The protocol starts with optimizing spectral regions, one per detector, so that different molecules become best distinguishable. Experimentally, the spectral regions are extracted from the continuous spectrum using a custom‐made micro‐optical element. In the ray‐optics picture, it guides all rays in a spectral region onto the entrance of an optical fiber connected to one detector. The shape of the micro‐optical element is derived by applying Snell's law to the given geometrical boundary conditions. A proof‐of‐concept measurement using a dedicated demonstrator refractive optical element in combination with a continuous white‐light source is performed. Indeed, the element selects the correct spectral regions and couples the light into the correct fibers. For the example of the identification of single amino acids in a protein, the protocol leads to a higher correct identification rate. Therefore, this protocol is useful for such protein identification experiments as performed in the EU project ProID.
Source: crossref · Quality: baseline

Research context

Materials processing

OpenAlex topics: Spectroscopy Techniques in Biomedical and Chemical Research · Advanced biosensing and bioanalysis techniques · Biosensors and Analytical Detection

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institute of Applied Physics (APH) Karlsruhe Institute of Technology (KIT) Wolfgang‐Gaede‐Str. 1 76131 Karlsruhe Germany

3DMM2O‐Cluster of Excellence Karlsruhe Institute of Technology (KIT) and Heidelberg University 76128 Karlsruhe Germany

European Molecular Biology Laboratory European Bioinformatics Institute (EMBL‐EBI), Wellcome Genome Campus Hinxton CB10 1SD Cambridge UK

Department of Physics Umeå University Linnaeus väg 24 Umeå 901 87 Sweden

Department of Physics and Materials Science University of Luxembourg 162a avenue de la Faiëncerie Luxembourg 1511 Luxembourg

Plasmon Nanotechnology Istituto Italiano di Tecnologia (IIT) Via Morego 30 Genova 16163 Italy

Institute of Nanotechnology (INT) Karlsruhe Institute of Technology (KIT) P.O. Box 3640 76021 Karlsruhe Germany

Funding

Wenner-Gren Stiftelserna · HORIZON EUROPE European Innovation Council · Knut och Alice Wallenbergs Stiftelse · Kempestiftelserna · Vetenskapsrådet · Darwin College, University of Cambridge · European Molecular Biology Laboratory · Deutsche Forschungsgemeinschaft · H2020 European Research Council

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