journal article·2023-03

Glucose‐Responsive Enzymatic Cascade Microreactors in Gas‐Shearing Microfluidics Microcapsules

Qingli Qu, Weixia Cheng, Xiaoli Zhang, Hossein Ravanbakhsh, Guosheng Tang, Aying Zhou, Dong Pei, Ranhua Xiong, Chaobo Huang

Advanced Materials Technologies· Volume 8· Issue 5
Publisher page

Abstract

Abstract Enzymatic cascade reactions are essential for metabolic pathway and biological signal transduction in living cells. More and more attention has been paid to artificial microreactors, which mimic these efficient, methodical, and selective biological cascade catalytic systems in cells. Natural hydrogels such as alginate and chitosan are thought of as suitable materials for matching the chemical, physical, and mechanical properties with extracellular matrix. Inspired by the structures of eukaryotic cells, alginate/chitosan multicompartment microcapsules for enzymatic cascade reaction are developed using an oil‐free gas‐shearing method. Multiple enzymes as biocatalysts are encapsulated in the particles to resemble complex physiological reactions. Biocatalysts, including glucose oxidase and peroxidase in alginate Janus core as a model, sense the external hyperglycemic environment, resulting in insulin release following chitosan shell protonation. The microcapsules show an excellent recyclability, room‐temperature long‐term and anti‐proteolysis stability. The microreactor approach leads to the development of a multi‐enzymatic cascade reaction system and provides an ideal carbohydrate platform to further understand the biomimetic metabolic function of organelles and organs.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Innovative Microfluidic and Catalytic Techniques Innovation · Enzyme Catalysis and Immobilization · Hemoglobin structure and function

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Joint Laboratory of Advanced Biomedical Materials (NFU‐UGent) Jiangsu Co‐Innovation Center of Efficient Processing and Utilization of Forest Resources College of Chemical Engineering Nanjing Forestry University (NFU) Nanjing 210037 P. R. China

CAS Key Laboratory of Chemistry of Northwestern Plant Resources Key Laboratory for Natural Medicine of Gansu Province Lanzhou Institute of Chemical Physics Chinese Academy of Sciences Lanzhou 730000 P. R. China

Qingdao Center of Resource Chemistry & New Materials Qingdao 266100 P. R. China

Children's Hospital of Nanjing Medical University Nanjing 210008 P. R. China

Department of Biomedical Engineering The University of Akron Akron OH 44325 USA

NMPA & Guangdong Key Laboratory of Molecular Target & Clinical Pharmacology State Key Laboratory of Respiratory Disease School of Pharmaceutical Sciences The Fifth Affiliated Hospital Guangzhou Medical University Guangzhou 511436 P. R. China

Funding

National Natural Science Foundation of China · Nanjing Forestry University

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