DataVXAdvanced Materials TechnologiesPaper
journal article·2023-06Open access

Aptamer‐Capped Nanoporous Anodic Alumina for SARS‐CoV‐2 Spike Protein Detection

Isabel Caballos, María Nieves Aranda, Alba López‐Palacios, Luis Pla, Sara Santiago‐Felipe, Andy Hernández‐Montoto, María Ángeles Tormo‐Mas, Javier Pemán, María Dolores Gómez‐Ruiz, Eva Calabuig, Beatriz Sánchez‐Sendra, Clara Francés‐Gómez, Ron Geller, Elena Aznar, Ramón Martínez‐Máñez

Advanced Materials Technologies· Volume 8· Issue 11
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Source abstract

Abstract

Abstract The COVID‐19 pandemic, which began in 2019, has highlighted the importance of testing and tracking infected individuals as a means of mitigating the spread of the virus. In this context, the development of sensitive and rapid methods for the detection of SARS‐CoV‐2, the virus responsible for COVID‐19, is crucial. Herein, a biosensor based on oligonucleotide‐gated nanomaterials for the specific detection of SARS‐CoV‐2 spike protein is presented. The sensing system consists of a nanoporous anodic alumina disk loaded with the fluorescent indicator rhodamine B and capped with a DNA aptamer that selectively binds the SARS‐CoV‐2 spike protein. The system is initially evaluated using pseudotype virus systems based on vesicular stomatitis virus carrying different SARS‐CoV‐2 S‐proteins on their surface. When the pseudotype virus is present, the cap of the solid is selectively removed, triggering the release of the dye from the pore voids to the medium. The nanodevice demonstrated its ability to detect pseudotype virus concentrations as low as 7.5·103 PFU mL. In addition, the nanodevice is tested on nasopharyngeal samples from individuals suspected of having COVID‐19.
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Research context

Nanomaterials

OpenAlex topics: SARS-CoV-2 detection and testing · Advanced biosensing and bioanalysis techniques · Biosensors and Analytical Detection

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico Universitat Politècnica de València Universitat de València Camino de Vera s/n 46022 Valencia Spain

Unidad Mixta de Investigación en Nanomedicina y Sensores. Universitat Politècnica de València Instituto de Investigación Sanitaria La Fe (IISLAFE) Avenida Fernando Abril Martorell Valencia 106, 46026 Spain

CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER‐BBN) Instituto de Salud Carlos III Valencia Spain

Grupo Infección Grave Hospital Universitari i Politècnic La Fe Instituto de Investigación Sanitaria La Fe (IISLAFE) Avenida Fernando Abril Martorell Valencia 106, 46026 Spain

Servicio de Microbiología Grupo Infección Grave Hospital Politècnic i Universitari La Fe. Instituto de Investigación Sanitaria La Fe (IISLAFE) Avenida Fernando Abril Martorell Valencia 106, 46026 Spain

Unidad de Enfermedades Infecciosas Hospital Politècnic i Universitari La Fe. Instituto de Investigación Sanitaria La Fe (IISLAFE) Avenida Fernando Abril Martorell Valencia 106, 46026 Spain

I ^{2}SysBio Universidad de Valencia‐CSIC. Calle del Catedrático Agustín Escardino 9 Paterna 46980 Spain

Funding

Generalitat Valenciana · European Commission · Banco Santander

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