DataVXAdvanced Materials TechnologiesPaper
journal article·2016-11

Online SERS Quantification of Staphylococcus aureus and the Application to Diagnostics in Human Fluids

Carme Catala, Bernat Mir‐Simon, Xiaotong Feng, Celia Cardozo, Nicolas Pazos‐Perez, Elena Pazos, Sara Gómez‐de Pedro, Luca Guerrini, Alex Soriano, Jordi Vila, Francec Marco, Eduardo Garcia‐Rico, Ramon A. Alvarez‐Puebla

Advanced Materials Technologies· Volume 1· Issue 8
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Source abstract

Abstract

Staphylococcus aureus is a common cause of serious infections. One of the main drawbacks in its treatment is the time required for a positive diagnosis, over 24 h, as most methods are still based in bacterial culture. Herein, a microfluidic optical device for the rapid and ultrasensitive quantification of S. aureus in real human fluids is designed. In this approach, the surface‐enhanced Raman scattering (SERS)‐encoded particles, functionalized with either an antibody or an aptamer, form a dense collection of electromagnetic hot spots on the surface of S. aureus. This allows for an exponentially increase of the SERS signal when particles accumulate on the microorganism as compared to their free condition in bulk solution. Quantification is achieved by passing the sample through a microfluidic device with a collection window where a laser interrogates and classifies each of the induced bacteria–nanoparticle aggregates in real time. Further, the advantages of using aptamers versus antibodies as biorecognition elements are extensively investigated.
Source: crossref · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Biosensors and Analytical Detection · Bacterial Identification and Susceptibility Testing

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Universitat Rovira i Virgili and Centro de Tecnología Química de Cataluña Carrer de Marcel·lí Domingo s/n 43007 Tarragona Spain

Medcom Advance S.A Av. Roma 08840 Barcelona Spain

Department of Surgery UD‐Vall d'Hebron School of Medicine Universitat Autònoma de Barcelona 08035 Barcelona Spain

Department of Infectious Diseases Hospital Clínic and School of Medicine University of Barcelona 08036 Barcelona Spain

Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) University of Barcelona 08036 Barcelona Spain

Department of Clinical Microbiology Hospital Clinic and School of Medicine University of Barcelona 08036 Barcelona Spain

ISGlobal Barcelona Ctr. Int. Health Res. (CRESIB) Hospital Clínic Universitat de Barcelona 08036 Barcelona Spain

Department of Clinical Oncology Hospital Univesitario Hm Madrid‐Torrelodones 28250 Torrelodones Madrid Spain

ICREA Passeig Lluís Companys 23 08010 Barcelona Spain

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