DataVXAdvanced Materials InterfacesPaper
journal article·2024-10Open access

Using Laponite to Deliver BMP‐2 for Bone Tissue Engineering – In Vitro, Chorioallantoic Membrane Assay and Murine Subcutaneous Model Validation

Karen M. Marshall, Jonathan P. Wojciechowski, Cécile Echalier, Sebastien J. P. Callens, Tao Yang, Øystein Øvrebø, Kun Zhou, Vineetha Jayawarna, Janos M. Kanczler, Molly M. Stevens, Jonathan I. Dawson, Richard O.C. Oreffo

Advanced Materials Interfaces· Volume 11· Issue 29
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Source abstract

Abstract

Abstract Fracture non‐union occurs due to various factors, leading to the development of potentially substantial bone defects. While autograft and allograft are the current gold standards for non‐union fractures, challenges related to availability and immune rejection highlight the need for improved treatments. A strategy in bone tissue engineering is to harness growth factors to induce an effect on cells to change their phenotype, behavior and initiate signaling pathways which lead to increased matrix deposition and tissue formation. Bone morphogenetic protein‐2 (BMP‐2) is a potent osteogenic growth factor however, given its rapid clearance time in vivo, there is a specific therapeutic window for efficacy while avoiding potential deleterious side‐effects. It is demonstrated that a Laponite nanoclay coating on a 3D printable and bioresorbable poly(caprolactone) trimethacrylate‐based resin enables binding of BMP‐2, decreases the rate of release, enabling reduced concentrations to be used while enhancing osteoinduction in both in vitro and in vivo models.
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Research context

NanomaterialsBiomaterialsMaterials processing

OpenAlex topics: Bone Tissue Engineering Materials · 3D Printing in Biomedical Research · biodegradable polymer synthesis and properties

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Bone and Joint Research Group Centre for Human Development Stem Cells and Regeneration Institute of Developmental Sciences University of Southampton Southampton SO16 6YD United Kingdom

Department of Materials Department of Bioengineering and Institute for Biomedical Engineering Imperial College London London SW7 2AZ United Kingdom

Department of Physiology, Anatomy and Genetics Department of Engineering Science, and Kavli Institute for Nanoscience Discovery University of Oxford Oxford OX1 3QU United Kingdom

Centre for the Cellular Microenvironment Advanced Research Centre University of Glasgow Glasgow G11 6EW United Kingdom

Funding

University of Southampton · Biotechnology and Biological Sciences Research Council · UK Regenerative Medicine Platform · National Institute for Health and Care Research

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