Long‐Term Culture of Human Pluripotent Stem Cells in Xeno‐Free Condition Using Functional Polymer Films

Abstract Human pluripotent stem cells (hPSCs), encompassing human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), hold immense potential in regenerative medicine, offering new opportunities for personalized cell therapies. However, their clinical translation is hindered by the inevitable reliance on xenogeneic components in culture environments. This study addresses this challenge by engineering a fully synthetic, xeno‐free culture substrate, whose surface composition is tailored systematically for xeno‐free culture of hPSCs. A functional polymer surface, pGC2 (poly(glycidyl methacrylate‐grafting‐guanidine‐co‐carboxylic acrylate)), offers excellent cell‐adhesive properties as well as non‐cytotoxicity, enabling robust hESCs and hiPSCs growth while presenting cost‐competitiveness and scalability over Matrigel. This investigation includes comprehensive evaluations of pGC2 across diverse experimental conditions, demonstrating its wide adaptability with various pluripotent stem cell lines, culture media, and substrates. Crucially, pGC2 supports long‐term hESCs and hiPSCs expansion, up to ten passages without compromising their stemness and pluripotency. Notably, this study is the first to confirm an identical proteomic profile after ten passages of xeno‐free cultivation of hiPSCs on a polymeric substrate compared to Matrigel. The innovative substrate bridges the gap between laboratory research and clinical translation, offering a new promising avenue for advancing stem cell‐based therapies.